• KSBB Journal
• /
• v.6 no.1
• /
• pp.1-7
• /
• 1991

To produce economically important indole alkaloids by cell cultures, we selected protoplastsderived clones (protoclones) of vinca (Catharanthus roseus) for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension-cultured cells. The highest plating efficiency (1%) was obtained when protoplasts were plated at a density of 1$\times$105 protoplasts/ml in a culture medium solidified with 0.4% Seaplaque agarose. The growth rates of 40 protoclones subcultured on a solid medium varied over a wide range. Protoclone VPC-6, which had the highest growth rate, was observed to produce relatively high yields of catharanthine and ajmalicine when cultured in a liquid medium. Although the original cell line did not produce catharanthine at a detectable level by HPLC, protoclone VPC-10 produced it at a level of 5.9$\mu\textrm{g}$/g fresh weight of cells for 10 days of culture. Under the same conditions, protoclone VPC-15 produced ajmalicine at a level of 133.6$\mu\textrm{g}$/g, of which productivity was improved about ,3 times than that of the original cell line. The results indicate that differences in the growth rate and indole alkaloid yield among the protoclones reflect the somaclonal variation in suspnsion-cultured cells.

• Journal of the Korean Society for Precision Engineering
• /
• v.27 no.10
• /
• pp.84-92
• /
• 2010

In this study a numerical analysis has been conducted for the flow characteristics and pumping performance of a piezoelectric-based micropump with electromagnetic resistance exerted on electrically conducting fluid. Here, electromagnetic resistance is alternately applied at the inlet and outlet with alternately applied magnetic fields in association with the reciprocal membrane motion of the piezoelectric-based micropump. A model of Prescribed Deformation is used for the description of the membrane motion. The internal flow characteristics and pumping performance are investigated with the variation of magnetic flux density, tube size, displacement of membrane and the frequency of the membrane. It turns out that the current micropump has a wide range of pumping flow rate compared with diffuser-nozzle based micropumps.

• Water Engineering Research
• /
• v.6 no.2
• /
• pp.73-89
• /
• 2005

Two kinds of high resolution GCMs with the same spatial resolutions but with different schemes run by domestic and foreign agencies are used to clarify the usefulness and sensitivity of GCM for water resources applications for Korea. One is AMIP-II (Atmospheric Model Intercomparison Project-II) type GCM simulation results done by ECMWF (European Centre for Medium-Range Weather Forecasts) and the other one is AMIP-I type GCM simulation results done by METRI (Korean Meteorological Research Institute). Observed mean areal precipitation, temperature, and discharge values on 7 major river basins were used for target variables. Monte Carlo simulation was used to establish the significance of the estimator values. Sensitivity analyses were done in accordance with the proposed ways. Through the various tests, discrimination condition is sensitive for the distribution of the data. Window size is sensitive for the data variation and the area of the basins. Discrimination abilities of each nodal value affects on the correct association. In addition to theses sensitivity analyses results, we also noticed some characteristics of each GCM. For Korean water resources, monthly and small window setting analyses are recommended using GCMs.

• Journal of Animal Environmental Science
• /
• v.21 no.2
• /
• pp.55-64
• /
• 2015

This survey was conducted to give the basic information and temperature and humidity variation by cooling systems in dairy farms in Korea. A total of 36 farms were surveyed in Gyeonggi province with the general information of farms such as milk production, and milk quality, types of cooling systems, and its operation method of dairy farms. All of surveyed farms have cooling fan and some have 1 more cooling systems such as sprinkler, foggy systems, ventilation duct, and shower system. Although the indoor THI of cow house was decreased by either ventilation duct or foggy system during hot season, the range belongs to mild heat stress scale for dairy cows.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.42 no.4
• /
• pp.59-66
• /
• 2000

This study was carried out to investigated the water requirement of twisted sweet peppers which are cultivated in a green house. The meteorological conditions during the experiment period was close to that of normal year except the temperature and relative humidity. The growth status was improved with the increased saturation ratio. The range of the variation of daily water requirement were very large. The peak consumption occurred in the early August. And the higher saturation ratio resulted in higher water requirement. The total water requirement were about 57.180g/d/plant for pot with 100% (P100) of saturation , about 38.700g/d/plant for pot with 80%(P80) of saturation , about 23,720g/d/plant for pot with 60%(P60) of saturation, and about 53, 390g/d/plant for field cultivation in the green house, respectively. The water requirement was correlated with average ambient temperature and growing status, while no significant correlation were found between water requirement and minimum relative humidity or intensity of solar radiation. And the higher correlation was shown as the saturation ratio was increased. The transpiration coefficients of twisted sweet pepper were 378.0g/g for field cultivation in the green house, 363.3g/g for P100, 338.7g for P80 which was the smallest among pot cultivation , and 472.1g/g for P60 , respectively.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.16 no.2
• /
• pp.142-149
• /
• 2004

Recently, the government introduced the thermal storage system for reducing the electric power load. Especially, the ice slurry type has gained lots of interest due to its good heat transfer and flowing characteristics. This study was peformed to understand the effects of transporting ice slurry through elbows of various angle. Propylene glycol water solution was used and about 2 mm ice particles were circulated. The experiments were carried out under various conditions, such as concentration and velocity of water solution ranging between 0∼20 wt％, 1.5∼2.5 m/s, respectively. And elbows with 4 different angles of 30$^{\circ}$, 45$^{\circ}$, 90$^{\circ}$, 180$^{\circ}$. The differential pressure and IPF (ice packing factor) between the pipe entry and exit were measured. The tendency of pressure loss and outlet IPF in elbow is that the pressure loss was reduced as concentration and flow velocity of water solution is increased, and low value appeared at 10 wt％ and 2.5 m/s. The variation of outlet IPF was compared with the inlet IPF in the range of $\pm$20％.

• Ocean and Polar Research
• /
• v.23 no.1
• /
• pp.23-34
• /
• 2001

Geochemical and Sr-Nd isotopic compositions and K-Ar ages are analyzed in volcanic rocks from Weno Island, Caroline Islands. Seven Weno lava samples of alkali basalt and basaltic trachyandesite are aphyric or sparsely phyric comprising olivine, plagioclase, and clinopyroxene phenocrysts. Whole-rock geochemical variation of Weno lavas reflects main fractional crystallization of olivine and Cr-spinel phenocrysts. Newly determined K-Ar ages of Weno lavas range from 6.7 to 11.3 Ma (late Miocene), indicating their formation during primary volcanic stage of Chuuk Islands. Trace element compositions of Weno lavas are very similar to those of typical ocean island basalts (OIBs), suggesting their formation during intra-plate mantle plume activity. The plume composition is isotopically very similar to that of Hawaiian hot spot. However, the age span of Chuuk volcanism is longer than that of the other individual volcanoes in the Pacific.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.8 no.1
• /
• pp.120-139
• /
• 1996

Experiment was performed to study the heat transfer characteristics in 27 kinds of 15 : 1 scale models of multi-louverred fin heat exchangers with a wide range of variables(R $e_{Lp}$ =100~1, 800, $L_p$/F$p$=0.3~0.9, $\theta$=20$^{\circ}$~40$^{\circ}$). Thermofoil heaters were used to heat the louver fins and the local average Nusselt number for each louver in the louver array was obtained at constant wall temperature conditions. Correlations are developed to predict the heat transfer characteristics and drag coefficients. Generally, the heat transfer characteristics in the multi-louvered fins is shown to be similar to those of the laminar heat transfer on a flat plate. As the Reynolds number, the louver pitch to fin pitch ratio$L_p$/F$p$and the louver angle($\theta$) increase respectively, the average Nusselt number increases, but the variation of average Nusselt number as a function of the louver angle is smaller than that as a function of the louver pitch to fin pitch ratio. In case of$L_p$/F$p$ <0.5, the average Nusselt number of the 3rd louver is especially lower than the others, it is expected that it is due to the flow structure such as a recirculation flow and a flow separation.

• Journal of Korean Port Research
• /
• v.15 no.1
• /
• pp.57-74
• /
• 2001

In the past, the predictions of beach processes and harbor sedimentation were mainly relied on the hydraulic model tests and empirical methods. In recent years, however, as computers have come into wide use, more accurate models have gradually been developed and thus replaced those conventional methods. For prediction of topographical change near the coastal area, we need informations of wave and current conditions in the numerical model which should be calculated in advance. Numerical model introduced in this study combines wave refraction-diffraction, breaking, bottom friction, lateral mixing, and critical shear stress and three sub-models for simulating waves, currents, and bottom change were briefly discussed. Simulations of beach processes and harbor sedimentation were also described at the coast neighboring Bangpo Harbor, Anmyundo, Chungnam, where the area has suffered accumulation of drifting sand in a small fishing harbor with a wide tidal range. We also made model test for the new layout of the harbor and planned south breakwater for preventing intrusion of sand. Although the model study gave reasonable description of beach processes and harbor sedimentation mechanism, it is necessary to collect lots of field observation data, including waves, tides and bottom materials, etc. for better prediction.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.14 no.9
• /
• pp.773-778
• /
• 2001

In this dissertation the results of the combined experimental and theoretical studies designed to understand and predict the spatial growth and transport coefficients for electrons in SF$_{6}$ and SF$_{6}$-Ar mixtures have described. The ionization and attachment coefficients in pure SF$_{6}$ and SF$_{6}$-Ar mixtures have been calculated over the range of 10$_{6}$ molecule and for Ar atom proposed by other authors. The transport coefficients for electrons in (0.2%)SF$_{6}$-Ar and (0.5%)SF$_{6}$-Ar mixtures were measured by time-of-flight method, and the electron energy distribution function and the parameters of the velocity and the diffusion were determined by the variation of the collision cross-sections with energy. The results obtained in this work will provide valuable information on the fundamental haviors of electrons in weakly ionized gases and the role of electron attachment in the choice of better gases and unitary gas dielectrics or electro negative components in dielectric gas mixtures. gas mixtures.