• 한국동물학회지
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• 제31권1호
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• pp.1-10
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• 1988

자매종 R.nigromaculate(참개구리)와 R. plancyi(금개구리)간의 생식적 격리기작을 구명하기 위하여 전라남도 광주시에서 발견된 자연집중 1개체를 포함한 두 종의 형태, 유전적 변이 및 번식시기 등을 조사한 분석한 결과는 다음과 같다. 두 종은 형태,유전,mating call 및 번식시기 등에 뚜렷한 차이가 있었고, 약 350만년 전에 남방계인 R. plancyi에서 R.nigromaculate가 분화되었을 것으로 추정되었다. 잡종 1개체는 전기영동 결과 일대잡종으로 확인되었으며 포란한 정상 난소를 갖고 있는 점으로 미루어 보아 생식이 간으하다고 여기지며, 따라서 postmating isolating mechanism은 불완전하게 생성되었다고 추측된다.

• The Korean Journal of Ecology
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• 제21권3호
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• pp.269-276
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• 1998

The study of the Rana plancyi, was done during the calling period at a pond in Osong, Chungbuk, Korea from May to the middle of August of 1996 and 1997. Five basic types of call-A, B, C, D, E, - were identified in Rana plancyi according to the structure of call types. The structure of A type call has only one pulse, B type call has two pulses and C, D type call has one separated pulse which is called introductory call and pulse group as follow it. On the other hand, E type call has 3-6 separate pulses. The interval between introductory call and pulse group is $0.73{\pm}0.29$ s(n=159) in C type call and $0.60{\pm}0.21$ s (n=48) in D type call. The number of pulses in the pulse group is $30.08{\pm}8.69$ in C type call and $15.78{\pm}2.40$ in D type call. An increase in water temperature induces a decrease in C and D type call duration (C type call r= -0.4153, p<0.001, D type call r=-0.7064, p<0.001). In case of C and D type call, the interval between introductory call and pulse group influenced more call duration than pulse group duration. We regarded A, B type call as a territorial call and C, D type call as a mating call and we recognized that E type call has the function of threat or alarm.

• 환경생물
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• 제27권1호
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• pp.110-113
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• 2009

In recent years, there has been a rapid global decline in amphibian populations, and infectious diseases have been associated with this decline. Diseased Gold-spotted pond frogs (Rana plancyi chosenica) were collected from a frog farm in Korea and identified using morphological and molecular analysis to identify the disease. The typical symptoms of ranaviral infection were observed in the tadpoles and adults frogs. The nucleotide sequence analysis revealed that the sequences showed the closest similarity with sequences to Frog virus 3, which belongs to the genus Ranavirus.

• 환경생물
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• 제25권1호
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• pp.8-15
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• 2007

본 연구는 금개구리(Rana plancyi chosenica)유생의 성장에 효율적인 먹이를 개발하기 위하여, 서로 다른 네가지 형의 먹이를 공급하고 그에 따른 성장을 비교한 것이다. 금개구리 유생에게 식물성 먹이를 공급한 군과 식물성과 동물성 배합 먹이를 공급한 실험군이 습지에서 수거한 유기물과 식물성 먹이 만을 공급한 대조군보다 더 빠른 성장을 보였다. 나아가, 식물성과 동물성 먹이를 혼합하여 공급한 먹이군이 순수하게 식물성 먹이 만을 공급한 실험군보다 더 높은 성장을 보였다. 식물성먹이에 동물성 먹이로서 열대어 사료를 혼합하여 공급한 실험군의 유생들이 대조군이나, 식물성 먹이 실험군, 식물성에 뱀장어 사료를 혼합하여 공급한 실험군 모두에 비하여 빠른 시간 안에 변태에 이르렀다. 이러한 결과는 식물성 먹이와 동물성 먹이를 적절하게 배합한 먹이가 금개구리 유생의 빠른 성장과 변태에 가장 효율적이라는 것을 나타낸다.

• Journal of Ecology and Environment
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• 제30권4호
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• pp.357-361
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• 2007

To determine the feeding sites preferred by adult gold-spotted pond frogs, Rana plancyi chosenica, and the foods that induce favorable growth of the frogs in the laboratory, we conducted two separate experiments between 27 May and 12 July 2007 in a vivarium. In the first experiment, we counted the number of crickets eaten by four gold-spotted pond frogs in a 60 min period at four different feeding sites within the experimental arenas: on the water surface, at the edge of a pond, and at two terrestrial sites. Adult gold-spotted pond frogs ate more crickets on the water surface and at the edge of the pond than the terrestrial sites. In the second experiment, we measured the growth of SVL (snout-vent length) and body mass of adult gold-spotted pond frogs fed crickets, mealworms, maggots, or earthworms in individual experimental boxes over a one month period. The SVL and body mass of the adult gold-spotted pond frogs fed crickets, mealworms, or maggots were greater than those of the frogs that were fed earthworms. These results indicate that providing crickets, mealworms, or maggots on the water or at the edge of a pond should induce favorable growth of captive-reared adult gold-spotted pond frogs.

• 한국동물학회지
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• 제23권4호
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• pp.263-272
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• 1980

개구리 2種 (Rana nigromaculata와 Rana plancyi chosenica)과 도룡뇽 (Hynobius leechii)의 胚發生에 EK른 lactate dehydrogenase (LDH)와 malate dehydrogenase (MDH)의 isozyme 組成變化를 polyacrylamide 電氣泳動法으로 조사 분석하고 이를 成體의 몇 器官과 비교하였다. R. nigromaculata에서 LDH의 B subunit의 合成을 지배하는 유전자는 heterozygous이고 H. leechii에서는 A subunit의 合成을 지배하는 유전자가 heterozygousfk고 추정된다. 위의 3種의 兩棲類\ulcorner 胚에서 發生初期에는 LDH-1 (심장형)의 活性이 높으나 發生이 진행됨에 따라 LDH-5 (근육형)의 活性도 점차 증가된다. MDH의 경우 發生初期부터 MDH-m과 MDH-s가 존재하고 발생 全段階를 통하여 그 組成에는 변화가 없으나 MDH-m의 活性이 점차 증가하는 경향을 보인다.

• Animal cells and systems
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• 제3권3호
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• pp.275-283
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• 1999

Korean R. plancyi occupies a restricted area in western South Korea and shows a relatively low level of genic variability (%P＝15.2, Ho=0.052, He＝0.048). In contrast, R. nigromaculata is broadly distributed in South Korea. The observed low level of variability of R. nigromaculata (%P＝14.3, Ho＝0.042, He＝0.043) is probably due to its recent colonization. Populations of R. nigromaculata exhibited considerable genetic differentiation (F$_{sT}$=0.149) and low level of gene flow (Nm＝1.427) among populations, compared to those of R. Plancyi (F$_{sTF$_{sT}$}$=0.096, Nm＝2.354), which occupies a restricted area. The observed levels of gene flow among populations of R. nigromaculata (Nm＝1.427) over a broad geographic range is relatively higher than other amphibian species. The high level of gene flow is probably the result of the high dispersal abilities of R. nigromaculata. A survey of temporal genic variation of R. nigromaculata showed that there was no significant change on the overall average genetic diversity from 1978 (average He＝0.044) to 1997 (average He＝0.040). Wright's F-statistics also indicated no significant genetic differentiation from 1978 (F$_{sT}$＝0.118) to 1997 (F$_{sT}$＝0.108). This suggests that the environmental change appears to have had little influence on the genetic composition of R. nigromaculata in the study areas during the past 20 years. The low level of temporal variation might be due to the result of high dispersal abilities and wide migration range of this species.

• Animal cells and systems
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• 제4권2호
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• pp.117-121
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• 2000

Genetic relationships among six species of the genus Rana from Korea were investigated by complete nucleotide sequence analyses of mitochondrial cytochrome b gene (1143 bp). Based on Kimura-2-parameter distance, the interspecific sequence differences of cytochrome b gene within the genus Rana were ranged from 7.83% to 25.00%. The genetic distances were 7.83% between R. nigromaculata and R. plancyi, 8.47% between two types of R. rugosa (type A and B), 10.42% between the brown frogs (R. amurensis and R. dybowskii), 16.11% between R. dybowskii types 1 and 2 and 12.36% between pond frogs (R. nigromaculata and R. plancyi) and R. catesbeiana. In the neighbor-joining and parsimony trees, R. catesbeiana was more closely related to pond frogs than brown frogs. R. dybowskii types 1 and 2 were considered to be at a distinct and specific level of differentiation (16.11%), while two types of R. rugosa were suspected to be at a subspecific level (8.47%).

• Animal Systematics, Evolution and Diversity
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• 제24권1호
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• pp.25-32
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• 2008

The Gold-spotted pond frog, Rana plancyi chosenica, designated as a vulnerable species by IUCN Red list. This species is a typical example facing local population threats and extinction due to human activities in South Korea. A strategic conservation plan for this endangered species is urgently needed. In order to provide information for future conservation planning, accurate information on the genetic diversity and taxonomic status is needed for the establishment of conservation units for this species. In this study, we used a molecular genetic approach using the mitochondrial cytochrome b gene and control region sequences to find the genetic diversity of gold-spotted pond frogs within South Korea. We sequenced the mitochondrial DNA cytochrome b gene and control region of 77 individuals from 11 populations in South Korea, and one from Chongqing, China. A total of 15 cytochrome b gene haplotypes and 34 control region haplotypes were identified from Korean gold-spotted pond frogs. Mean sequence diversity among Korean gold-spotted pond frogs was 0.31% (0.0-0.8%) and 0.51% (0.0-1.0%), respectively. Most Korean populations had at least one unique haplotype for each locus. The Taean, Ansan and Cheongwon populations had no haplotypes shared with other populations. There was a sequence divergence between Korean and Chinese gold-spotted pond frogs (1.3% for cyt b; 2.9% for control region). Analysis of genetic distances and phylogenetic trees based on both cytochrome b and control region sequences indicate that the Korean gold-spotted pond frog are genetically differentiated from those in China.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.119.1-119
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• 1997

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