• 식물조직배양학회지
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• 제27권6호
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• pp.491-496
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• 2000

더덕의 효과적인 재분화조건 (약 90%의 재분화율)을 바탕으로 개화시기 조절유전자인 RAG25 Codonopsis lanceolata유전자의 도입을 시도하였다. 더덕의 잎 절편을Agrobacterium과 3일 동안 공조배양 하였으며 NAA 2 mg/L, BA 2 mg/L (N2B2)와 NAA 2 mg/L, BA 4 mg/L (N2B4)가 첨가된 shoot inducing media (Km 20mg/L와 Cf 250 mg/L가 첨가됨)에서 배양하였을 때 형질전환 신초가 형성되었다. 획득된 신초에서 genomic DNA를 추출하고 npt II와 RAG25 primer를 이용한 PCR을 수행하여 각각 0.7 kb와 0.6 kb의 band를 확인하였다. RAG25 유전자에 대한 PCR산물을 이용하여 Southern hybridization을 수행한 결과 PCR과 동일한 위치에서 band 가 검출되어져 이 유전자의 도입을 재확인하였다.

• BMB Reports
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• 제40권3호
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• pp.432-438
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• 2007

V(D)J recombination, a site-specific gene rearrangement process occurring during the lymphocyte development, begins with DNA double strand breaks by two recombination activating gene products (RAG1/2) and finishes with the repair process by several proteins including DNA-dependent protein kinase (DNA-PK). In this report, we found that RAG2 was specifically phosphorylated by DNA-PK at the $365^{th}$ serine residue, and this phosphorylated RAG2 affected the V(D)J recombination activity in cells in the GFP expression-based assay. While the V(D)J recombination activity between wild-type RAG2 and mutant S365A RAG2 in the assay using a signal joint substrate was undistinguishable in DNA-PK deficient cells (M059J), the activity with wild-type RAG2 was largely increased in DNA-PK proficient cells (M059K) in comparison with mutant RAG2, suggesting that RAG2 phosphorylation by DNA-PK plays a crucial role in the signal joint formation during V(D)J recombination.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2024년도 춘계학술발표대회
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• pp.792-793
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• 2024

RAG는 정보 검색과 셍성 모델을 결합하여 주어진 주제나 질문에 관련된 지식을 생성하는 방법이다. 본 연구는 RAG의 성능을 높이기 위해 문서 내 문장의 평균 길이에 따른 청크의 크기와 오버랩 크기를 비교하여 최적화한다. 이를 통해 참조 문서의 특징에 맞춘 RAG를 개발할 수 있고, 다양한 종류의 글에 대해 맞춤형 답변을 제공할 수 있을 것으로 예상된다.

• Laboraroty Animal Research
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• 제34권4호
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• pp.166-175
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• 2018

Recombination activating gene-2 (RAG-2) plays a crucial role in the development of lymphocytes by mediating recombination of T cell receptors and immunoglobulins, and loss of RAG-2 causes severe combined immunodeficiency (SCID) in humans. Rag-2 knockout mice created using homologous recombination in ES cells have served as a valuable immunodeficient platform, but concerns have persisted on the specificity of Rag-2-related phenotypes in these animals due to the limitations associated with the genome engineering method used. To precisely investigate the function of Rag-2, we recently established a new Rag-2 knockout FVB mouse line ($Rag-2^{-/-}$) manifesting lymphopenia by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease. In this study, we further characterized their phenotypes focusing on histopathological analysis of lymphoid organs. $Rag-2^{-/-}$ mice showed no abnormality in development compared to their WT littermates for 26 weeks. At necropsy, gross examination revealed significantly smaller spleens and thymuses in $Rag-2^{-/-}$ mice, while histopathological investigation revealed hypoplastic white pulps with intact red pulps in the spleen, severe atrophy of the thymic cortex and disappearance of follicles in lymph nodes. However, no perceivable change was observed in the bone marrow. Moreover, our analyses showed a specific reduction of lymphocytes with a complete loss of mature T cells and B cells in the lymphoid organs, while natural killer cells and splenic megakaryocytes were increased in $Rag-2^{-/-}$ mice. These findings indicate that our $Rag-2^{-/-}$ mice show systemic lymphopenia with the relevant histopathological changes in the lymphoid organs, suggesting them as an improved Rag-2-related immunodeficient model.

• 한국의상디자인학회지
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• 제14권3호
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• pp.67-77
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• 2012

Hanbok of dolls can be a good medium that can given with value of traditional cultural products, however, it is not easy to see hanbok and its pattern from dolls. Especially for the case of rag doll which is closely related to the life of users, it has enough value as traditional cultural contents, however, there have been not sufficient studies on its pattern development and classification of form of dolls. Therefore, by classifying the body type of dolls by its pose, this study aims to provide a basic data for the development of hanbok pattern. This study looks into the origin and meaning of dolls and the definition and features of rag doll, then, it collected pictures and data rag dolls produced by 29 domestic companies. Through the data collected, the six different types of dolls, 'Sitting Style', 'Standing Style', 'Lying Style', 'Cushion Style', 'Quadruped Sitting Style', 'Quadruped Standing Style', were classified into form. In the future, I hope the result of this study can be used as useful data for toy manufactures and cultural business in relation to development of rag doll and at the same time as a basic data for development of hanbok pattern development of rag dolls as traditional cultural goods.

• BMB Reports
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• 제36권2호
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• pp.201-206
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• 2003

Two lymphoid-specific proteins, RAG1 and RAG2, are required for the initiation of the V(D)J recombination in vitro. The V(D)J cleavage that is mediated by RAG proteins at the border between the coding and signal sequences results in the production of a hairpin at the coding end and a double-stranded break at the signal end. Two hairpin coding ends are re-opened, modified, and sealed; whereas, the signal ends are directly ligated. Here I report that only RAG1 can carry out a distinct endonucleolytic activity in vitro using an oligonucleotide substrate that is tethered by a short single-stranded DNA. The purified RAG1 protein alone formed a nick at the near position to the recombination signal sequence. This endonucleolytic activity was eliminated by immunoprecipitation using the RAG1-specific antibody, and required the 3'-hydroxy group. All of the RAG1 mutants that were incapable of the nick and hairpin formation in the V(D)J cleavage analysis also showed this new endonucleolytic activity. This suggests that the nicking activity that was observed might be functionally different from the nick formation in the V(D)J cleavage.

• 대한원격탐사학회지
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• 제18권2호
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• pp.107-116
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• 2002

Watershed 알고리듬을 통해 에지 기반과 영역 기반 기법을 결합한 하이브리드 영상 분할 알고리듬을 제안하였다. 먼저 minimax flow와 결합된 평균 곡률 확산을 이용하여 에지를 보존하면서 잡음을 제거를 수행한다. 영상을 watershed 알고리듬을 이용하여 분할한 후에 RAG (Region Adjacency Graph)을 사용하여 분할된 영역들간의 관계를 분석한다. RAG의 그래프 노드와 에지 비용은 분할된 영역과 두 인접한 영역사이의 상이함을 나타낸다. 최소 비용의 RAG의 에지를 찾아 가장 유사한 영역 쌍이 결정되면 두 영역은 서로 합치고 RAG은 갱신된다. 제안한 방법을 통해서 잡음을 효과적으로 감소시키고 한 화소 두께의, 닫힌 경계선을 획득할 수 있었다.

• 대한원격탐사학회지
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• 제22권6호
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• pp.601-612
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• 2006

본 연구는 방대한 크기 원격 탐사 영상 자료의 효율적인 분석을 위한 RAG (Region Adjancency Graph) 기반 영상 분할 기법을 제안하고 있다. 제안된 알고리즘은 계산의 효율성을 위하여 CN-chain 연결과 저장 기억의 효율성을 위하여 sliding 다중 창을 사용한다. RAG에 의한 지역 합병은 최선의 결합을 위한 edge을 발견과 합병에 따른 graph의 갱신의 과정이다. CN-chain 연결법은 가장 유사한 인접 지역의 연결을 형성하면서 최선의 edge를 발견하여 합병을 해 나가는 과정으로 영상 자료 크기의 증가에 따라 단지 증가 배수만큼만 분석 시간을 증가시킨다. 합병에 의해 변하는 RAG의 효율적인 갱신을 위하여 RNV(Regional Neighbor Vector)를 사용하였다. 방대한 크기 자료 분석은 막대한 기억 용량의 시스템을 필요로 한다. 제안된 수평적인 구조의 sliding 다중 창 작업은 필요한 기억 용량을 현저히 감소시켜 가능한 분석 자료의 크기를 증대시켰을 뿐 아니라 계산 시간의 감소를 초래하였다. 본 연구는 simulation 자료를 사용하여 광범위하게 제안된 알고리즘의 성능을 실험하였으며 실험 결과는 알고리즘의 효율성을 입증하였다.

• Journal of Microbiology and Biotechnology
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• 제18권12호
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• pp.1890-1894
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• 2008

Apoemulsan is a biopolymer with potent emulsification activity, produced by Acinetobacter venetian us RAG-1 (RAG-1). The wee gene cluster is responsible for apoemulsan biosynthesis. The analysis of (i) a putative polysaccharide copolymerase mutant (${\Delta}wzc$), (ii) a putative polymerase mutant (${\Delta}wzy$), and (iii) an apoemulsan-deficient variant (${\Delta}2$) indicated that the wee gene cluster controls the synthesis of two polysaccharides: high molecular weight (HMW) and low molecular weight (LMW). LMW polysaccharide of wee origin was present in LPS isolated from RAG-1 cells, suggesting a link to the Lipid A-core of LPS molecules. SDS-PAGE analysis indicated that apoemulsan is copurified with LPS polysaccharide, with implications in the emulsification activity of RAG-1 polymer.

• 한국수산과학회지
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• 제57권3호
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• pp.239-252
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• 2024

Recombination activating genes (RAGs) play a crucial role in initiating V(D)J recombination, which is essential for developing adaptive immunity in vertebrates. In this study, we cloned and characterized RAG1/2 cDNA from the marine medaka Oryzias dancena (OdRAG1/2) and investigated their mRNA expression patterns during ontogenetic developmental stages. The OdRAG1 and OdRAG2 cDNA contained open reading frames (ORFs) encoding proteins containing 1,078 and 531 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis revealed that OdRAG1 and OdRAG2 are highly conserved with their corresponding orthologs, featuring distinct core and non-core regions. Notably, expression analysis showed that, in contrast to other fish RAGs studied, OdRAG1/2 expression peaked at 0 days post-hatching (DPH). Additionally, for the expression of T and B cell differentiation markers, CD3γ and CD20, also peaked at 0 DPH. Collectively, adaptive immunity in O. dancena potentially begins during embryonic development, which is critical for V(D)J recombination and essential immune component development, suggesting the early ontogenetic stage interactions between innate and adaptive immunity.