• 제목/요약/키워드: Raccoon

검색결과 57건 처리시간 0.034초

Molecular characterizations of phosphoprotein of rabies virus circulating in Korea

  • Kim, Ha-Hyun;Yang, Dong-Kun;Jeon, Jeong Kuk;Cho, Soo-Dong;Song, Jae-Young
    • 대한수의학회지
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    • 제52권1호
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    • pp.9-18
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    • 2012
  • Rabies is a major zoonotic disease that causes approximately 55,000 human deaths worldwide on an annual basis. The nucleocapsid protein and glycoprotein genes of the Korean rabies virus (RABV) have been subjected to molecular and phylogenetic analyses. Although the phosphoprotein (P) has several important functions in viral infection and pathogenicity, the genetic characterizations of the P of Korean RABV isolates have not yet been established. In the present study, we conducted genetic analyses of P genes of 24 RABV isolates circulating in the Republic of Korea (hereafter, Korea) from 2008 to 2011. This study revealed that the P genes of Korean RABVs are genetically similar to those of RABV strains of lyssavirus genotype I including V739 (dogs, Korea), NNV-RAB-H (humans, India), NeiMeng925 (raccoon dogs, China), and RU9.RD (raccoon dogs, Russia). Among Korean isolates, the RABV P genes showed low variability in the variable domains among Korean isolates; they had specific consensus sequences and amino acid substitutions capable of identifying geographic characteristics and retained specific sequences thought to be important for viral function. These results provide important genetic characteristics and epidemiological information pertaining to the P gene of the Korean RABV.

Development of a Blocking ELISA for Measuring Rabies Virus-specific Antibodies in Animals

  • Yang, Dong-Kun;Kim, Ha-Hyun;Ryu, Jieun;Gee, Mi-ryun;Cho, In-Soo
    • 한국미생물·생명공학회지
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    • 제46권3호
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    • pp.269-276
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    • 2018
  • Rabies virus (RABV)-specific antibodies in animals and humans are measured using standard methods such as fluorescent antibody virus neutralization (FAVN) tests and rapid fluorescent focus inhibition tests, which are based on cell culture systems. An alternative assay that is safe and easy to perform is required for rapid sero-surveillance following mass vaccination of animals. Two purified monoclonal antibodies (4G36 and B2H17) against RABV were selected as capture and detection antibodies, respectively. A genetically modified RABV, the ERAGS strain, was propagated and concentrated by polyethylene glycol precipitation. Optimal conditions for the RABV antigen, antibodies, and serum dilution for a blocking enzymelinked immune sorbent assay (B-ELISA) were established. We evaluated the sensitivity, specificity, and accuracy of the B-ELISA using serum samples from 138 dogs, 71 raccoon dogs, and 25 cats. The B-ELISA showed a diagnostic sensitivity of 95.8-96.3%, specificity of 91.3-100%, and accuracy of 96.0-97.2% compared to the FAVN test. These results suggest that the B-ELISA is useful for sero-surveillance of RABV in dogs, raccoon dogs, and cats.

The outbreaks and surveillance of animal rabies in Gangwon-do

  • Shin Myung-Kyun;Shin Eun-Kyung;Chung Dong-Soo;Cheong Ki-Soo;Park Young-Nam;Choi Jee-Hee
    • 한국동물위생학회지
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    • 제27권4호
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    • pp.335-343
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    • 2004
  • Although human rabies deaths are rare, the disease remains a public health problem in Korea. Here we report the outbreaks and surveillance of animal rabies in Gangwon-do. Animal rabies infections were identified in 119 animals from 1993 to 2003. The $78\%$ of all rabid animals were domestic species in Gangwon-do. Wild Korean raccoon dog (N. p. koreensis) continued to be the only reported rabid wildlife species. Outbreaks of rabies infections in Korean raccoon dogs are found in broad geographic regions across the northern Gangwon-do. The principal rabies hosts today are probably wild animals in Gangwon-do. The malaise, cerebral dysfunction, anxiety, confusion, agitation and abnormal behavior of the animals were the important symptoms of the disease. The Encephalitis, infiltration with lymphocytes and polymorphonuclear leukocytes and the inclusion bodies (Negri bodies) in neuronal cells were the specific histopathological signs. The results of indirect fluorescent antibody test (IFA) for animal rabies diagnosis were identical and the technique was useful to diagnose the disease. Preexposure vaccination is recommended for persons in high-risk groups, such as laboratory workers, veterinarians and certain animal handlers.

전라남도 구례 농촌지역에서의 단기원격무선추적을 이용한 너구리(Nyctereutes procyonoides koreensis) 한 쌍의 행동권에 관한 연구 (A Brief Report of the Short-Term Home Range Study of a Pair of Raccoon Dogs(Nyctereutes procyonoides koreensis) in a Rural Area of Gurye, Chonnam Province, South Korea Using Radiotracking Method)

  • 김백준;최태영;박종화;김영준;이항
    • 한국환경생태학회지
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    • 제22권3호
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    • pp.230-240
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    • 2008
  • 이 연구의 주된 목적은 구례 농촌지역에 서식하는 1쌍의 너구리(Nyctereutes procyonoides koreensis) 행동권을 측정하고 이전 너구리 연구와 비교하는데 있다. 원격무선추적은 2개체의 너구리를 이용하여 2개월 간격(2000년 6, 8, 10, 12월)으로 2일씩 주기적으로 수행되었다. 모니터링 기간 동안, 무선추적은 주간과 야간에 걸쳐 보통 $1{\sim}3$시간 간격으로 실시되었다. 전체 $46{\sim}64$개의 무선추적 자료의 분석 결과는 95% 최소볼록다각형(MCP) 방법에서 1쌍의 너구리의 전체행동권 크기가 $0.41km^2$, 평균행동권 크기가 $0.32km^2$임을 보여주었다. 암컷과 수컷의 행동권은 상당 부분 중첩되었고(약 $70{\sim}95%$), 행동권 크기는 서로 유사한 양상을 나타내었다. 그러나 주간$(0.01km^2)$과 야간$(0.35km^2)$의 행동권 크기는 매우 큰 차이를 보였고, 여름$(0.56km^2)$에 가장 컸지만 겨울$(<0.01km^2)$에 가장 작았다. 추가적으로, 1쌍의 너구리는 1개의 핵심지역과 4개의 서로 다른 섭식지역들을 가지고 있었다. 결론적으로, 동일한 개체들을 이용하여 하루 동안 더 빈번한 추적 수와 더 긴 추적 간격을 이용한 이 너구리 행동권 자료는 하루 동안 덜 빈번한 추적 수와 더 짧은 추적 간격을 이용한 이전의 연구와 매우 유사한 결과를 보여 주었다.

성남시 갈마치 지역의 동물이동통로 복원을 위한 생태특성 분석 및 복원목표종의 선정 (Analysis of Ecological Characteristics and Selection of Target Species for Restoration of Wildlife Corridor at Galmachi of Sungnam City)

  • 전승훈;이상돈;하성룡
    • 환경영향평가
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    • 제20권1호
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    • pp.13-24
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    • 2011
  • This study was carried out to analyze ecological characteristics and select target species for effective restoration plans of wildlife corridors at Galmachi of Sungnam City. The actual vegetation in study area was physiognomically mixed with deciduous broad leaved forests dominated by oaks and artificially planted species. And it is a secondary forest formatted by sprout originated stand and being on early succession stage as a young stand. So study site was regarded as not suitable for the habitats for wildlife. The number of mammal species at study site was total 22 species, but most of these species were found as not so many in individual numbers. The individual number of hare was approximately 4 to 8, while the raccoon dog and water deer with 2 to 3 and 1 to 2, respectively. As such, it was estimated as a low population density due to discontinuation of ecosystem and the man-made impacts. In this study, raccoon dog and water deer were selected as the target species because of their migration patterns and ecological value in local ecosystem. It was suggested to establish a wildlife corridors of an over-bridge type at the peak area of Galmachi, known to be the mostly-used migratory route for 2 target species.

동물원(動物園)에서 사육중(飼育中)인 야생동물(野生動物)의 기생충감염(寄生蟲感染) 관(關)한 조사(調査) (Epizootiological Study on Infestation Rate of Parasites in Zoo Animals)

  • 박영재;이원창
    • 대한수의학회지
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    • 제17권1호
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    • pp.17-26
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    • 1977
  • Parasites of wild animals are closely related with parasites of domestic animals. Wild animals take charge of an important role at parasitic infestation of domestic animals because of unrestrained movement. The authors carried out the work of actual condition of parasitic infestation on wild animals, total 1,014 cases, in the Korean Zoo. The results are summarized as follows: 1. Total rate of parasitic infestation was 36.1% with infestation of 366 among 1,014 cases. The rate of single infestation was 32.6% with infestation of 331 cases, double infestation 3.1% with 31 cases, triple infestation 0.2% with 2 cases and quadrople infestation 0.2% with 2 cases. 2. The parasites on the zoo animals were identified as follows: Lion: Sarcoptiform, Toxocara sp., Toxascaris leonina, Ancylostoma sp. and Isospora spp. Puma: Toxocara sp., Ancylostoma sp. and Isospora sp. Leopard: Toxocara spp., Ancylostoma sp., Trichuris sp., Dibothriocephalus sp. and Physaloptera sp. Wolf: Sarcoptiform and Dibothriocephalus spp. Fox: Trichuris sp., Capillaria aerophila, Spirocerca sp., Paragonimas kellicotti. Jackal: Sarcoptiform, Ascaris sp. and Echinococcus granulosus. Wild Cat: Dibothriocephalus sp. Tiger: Toxascaris leonina. Bear: Sarcoptiform, Metastrongylus apri, Ancylostoma sp. and Ascaris sp. Raccoon and Raccoon dog: Sarcoptiform, Paragonimus kelliotti, and Isospora sp. Boar: Oesophagostomum spp. and Eimeria spp. Mortkey: Sarcoptiform, Trichuris sp., Physaloptera spp.. Enterobius sp. and Isospora sp. Elephant: Sarcoptiform, Strongyloides sp. and Strongylus spp. Deer: Sarcoptiform, Strongyloides sp., Trichuris ovis, Mccistocirrus digitatus, Haemonchus sp., Oesophagostomum radiatum, Paramphistornum spp., Bunostomum phlebotomum, Fasciola hepatica and Eimeria spp. Bison: Sarcoptiform, Haernonchus sp., Marshallagia sp., Nematodirus sp. and Eimeria sp. Zebra: Strongylus sp. and Parascaris equorum. Goral and Barbary: Sarcoptiform, Haemonchus sp., Oesophagostomum venulosum, Moniezia sp. and Eimeria spp. Lama: Strongyloides sp. and Haemonchus sp. Kangaroo: Strongyloides sp. and Haemonchus sp. Camel: Strongyloides sp., Trichuris ovis and Eimeria sp. Peacock and the Other Birds: Sarcoptiform, Capillaria contorta, Capillaria caudinflata, Ascaridia spp., Heterakis spp., Hymenolepis sp., Eimeria spp., Histomonas, Ornithionyssus bacoti, Macrochelidae and Trichomonas. 3. Among the zoo animals, wild carnivora were infestated with the parasites which are common parasites of dogs and cats, wild herbivora were infestated with the parasites of herbivora domestic animals. and wild fowls were infestated with the parasites of domestic fowls.

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시클로 덱스티린과 그 메틸유도체의 구조분석 (Conformational Analysis of Cyclodextrins and Their Methylated Analogs)

  • 최희숙
    • 대한화학회지
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    • 제36권2호
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    • pp.324-328
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    • 1992
  • ${\alpha}$-와 ${\beta}$-시클로덱스트린과 그의 메틸유도체들을 Chemical shift(${\delta}$)와 Coupling constant(J)를 470 MHz $^1H$ NMR을 이용해서 수용액 안에서 분석하였다. 정확한 ${\delta}$와 J값들을 얻기 위해서 Raccoon spin simulation program을 이용해서 실험 data를 분석하였다. 시클로덱스트린의 $C_{5-}C_6$ bond 주위의 rotamer의 분포를 $J_{56a}$$J_{56b}$값을 이용하여 실험 data를 분석하였다. 위 계산에 의해 ${\alpha}$-와 ${\beta}$-시클로덱스트린에서는 gg conformer가 가장 많이 존재하였고 tg conformer가 가장 적게 존재했다. 그러나 그 메틸 유도체에서는 gg conformer가 더 많이 증가하였고 gt conformer가 가장 적게 존재함을 알았다.

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명각으로 본 모직물의 개건 -고대부터 고려시대까지- (A DEVELOPMENT OF WOOL FABRICS FOR NAME KOREAN Wool Fabrics From Ancient To Koryo)

  • 박순지;이춘계
    • 복식
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    • 제21권
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    • pp.19-30
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    • 1993
  • There are record that ancient Korean WOOl fabrics male from sheep, camel, rabbit. bear, horse. calile, raccoon dog, fox and so on. Wool fiber fabriher were also reported Kye, Gal, China, Tap Dung, Kuyu anul ram. These wool fabrics wre named amongling to their fineness and weaving method in the fabric. Technology of wool fabric weaving was developed and from Sam Kuk dynasties to to Koryo Dynasty wool fabrics were reported to weave and trade to China, Japan and Arabia. These fabrics were mainly used as clothes, rugs or blankets. In Koryo Dynasty, especially Kye Kurn was used for trading goods to China. There is another record that two thousand sheep, camels offord Koryo by Yo and Kum Dynasty. In this study, the characteristics of Korean wool fabrics will be disscussed from the literature survey of the relevant references.

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