• 제목/요약/키워드: Raccoon

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Molecular characterizations of phosphoprotein of rabies virus circulating in Korea

  • Kim, Ha-Hyun;Yang, Dong-Kun;Jeon, Jeong Kuk;Cho, Soo-Dong;Song, Jae-Young
    • Korean Journal of Veterinary Research
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    • v.52 no.1
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    • pp.9-18
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    • 2012
  • Rabies is a major zoonotic disease that causes approximately 55,000 human deaths worldwide on an annual basis. The nucleocapsid protein and glycoprotein genes of the Korean rabies virus (RABV) have been subjected to molecular and phylogenetic analyses. Although the phosphoprotein (P) has several important functions in viral infection and pathogenicity, the genetic characterizations of the P of Korean RABV isolates have not yet been established. In the present study, we conducted genetic analyses of P genes of 24 RABV isolates circulating in the Republic of Korea (hereafter, Korea) from 2008 to 2011. This study revealed that the P genes of Korean RABVs are genetically similar to those of RABV strains of lyssavirus genotype I including V739 (dogs, Korea), NNV-RAB-H (humans, India), NeiMeng925 (raccoon dogs, China), and RU9.RD (raccoon dogs, Russia). Among Korean isolates, the RABV P genes showed low variability in the variable domains among Korean isolates; they had specific consensus sequences and amino acid substitutions capable of identifying geographic characteristics and retained specific sequences thought to be important for viral function. These results provide important genetic characteristics and epidemiological information pertaining to the P gene of the Korean RABV.

Development of a Blocking ELISA for Measuring Rabies Virus-specific Antibodies in Animals

  • Yang, Dong-Kun;Kim, Ha-Hyun;Ryu, Jieun;Gee, Mi-ryun;Cho, In-Soo
    • Microbiology and Biotechnology Letters
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    • v.46 no.3
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    • pp.269-276
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    • 2018
  • Rabies virus (RABV)-specific antibodies in animals and humans are measured using standard methods such as fluorescent antibody virus neutralization (FAVN) tests and rapid fluorescent focus inhibition tests, which are based on cell culture systems. An alternative assay that is safe and easy to perform is required for rapid sero-surveillance following mass vaccination of animals. Two purified monoclonal antibodies (4G36 and B2H17) against RABV were selected as capture and detection antibodies, respectively. A genetically modified RABV, the ERAGS strain, was propagated and concentrated by polyethylene glycol precipitation. Optimal conditions for the RABV antigen, antibodies, and serum dilution for a blocking enzymelinked immune sorbent assay (B-ELISA) were established. We evaluated the sensitivity, specificity, and accuracy of the B-ELISA using serum samples from 138 dogs, 71 raccoon dogs, and 25 cats. The B-ELISA showed a diagnostic sensitivity of 95.8-96.3%, specificity of 91.3-100%, and accuracy of 96.0-97.2% compared to the FAVN test. These results suggest that the B-ELISA is useful for sero-surveillance of RABV in dogs, raccoon dogs, and cats.

The outbreaks and surveillance of animal rabies in Gangwon-do

  • Shin Myung-Kyun;Shin Eun-Kyung;Chung Dong-Soo;Cheong Ki-Soo;Park Young-Nam;Choi Jee-Hee
    • Korean Journal of Veterinary Service
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    • v.27 no.4
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    • pp.335-343
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    • 2004
  • Although human rabies deaths are rare, the disease remains a public health problem in Korea. Here we report the outbreaks and surveillance of animal rabies in Gangwon-do. Animal rabies infections were identified in 119 animals from 1993 to 2003. The $78\%$ of all rabid animals were domestic species in Gangwon-do. Wild Korean raccoon dog (N. p. koreensis) continued to be the only reported rabid wildlife species. Outbreaks of rabies infections in Korean raccoon dogs are found in broad geographic regions across the northern Gangwon-do. The principal rabies hosts today are probably wild animals in Gangwon-do. The malaise, cerebral dysfunction, anxiety, confusion, agitation and abnormal behavior of the animals were the important symptoms of the disease. The Encephalitis, infiltration with lymphocytes and polymorphonuclear leukocytes and the inclusion bodies (Negri bodies) in neuronal cells were the specific histopathological signs. The results of indirect fluorescent antibody test (IFA) for animal rabies diagnosis were identical and the technique was useful to diagnose the disease. Preexposure vaccination is recommended for persons in high-risk groups, such as laboratory workers, veterinarians and certain animal handlers.

A Brief Report of the Short-Term Home Range Study of a Pair of Raccoon Dogs(Nyctereutes procyonoides koreensis) in a Rural Area of Gurye, Chonnam Province, South Korea Using Radiotracking Method (전라남도 구례 농촌지역에서의 단기원격무선추적을 이용한 너구리(Nyctereutes procyonoides koreensis) 한 쌍의 행동권에 관한 연구)

  • Kim, Baek-Jun;Choi, Tae-Young;Park, Chong-Hwa;Kim, Young-Jun;Lee, Hang
    • Korean Journal of Environment and Ecology
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    • v.22 no.3
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    • pp.230-240
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    • 2008
  • The primary aim of this study is to estimate the home range of a pair of raccoon dogs(Nyctereutes procyonoides koreensis) and to compare with the previous study of raccoon dogs in a rural area of Gurye, the southern part of South Korea. Radiotracking was regularly carried out on 2 raccoon dogs for 2 days every 2 months(in June, August, October and December, 2006). During the 2 days, radiotracking was usually conducted every $1{\sim}3$ hours through day and night. The analysis of tracking data with a total of $46{\sim}64$ bearings showed that the total home range size of the pair was $0.41km^2$, and mean home range size was $0.32km^2$ by 95% minimum convex polygons(MCP) estimate. The home ranges of the male and female were largely overlapping(about $70{\sim}95%$), and the sizes were not very much different from each other. However, there was a big difference between day $(0.01km^2)$ and night-time $(0.35km^2)$ home ranges, and it was largest in summer$(0.56km^2)$ and smallest in winter $(<0.01km^2)$. In addition, the home range of the pair included 1 core area and 4 different feeding areas. In conclusion, our raccoon dog home range data using the same individuals but with more frequent bearings per day and more extended tracking intervals still showed very similar results to the previous study with less frequent bearings per day and more extensive tracking days.

Analysis of Ecological Characteristics and Selection of Target Species for Restoration of Wildlife Corridor at Galmachi of Sungnam City (성남시 갈마치 지역의 동물이동통로 복원을 위한 생태특성 분석 및 복원목표종의 선정)

  • Chun, Seung-Hoon;Lee, Sang-Don;Ha, Sung-Ryong
    • Journal of Environmental Impact Assessment
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    • v.20 no.1
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    • pp.13-24
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    • 2011
  • This study was carried out to analyze ecological characteristics and select target species for effective restoration plans of wildlife corridors at Galmachi of Sungnam City. The actual vegetation in study area was physiognomically mixed with deciduous broad leaved forests dominated by oaks and artificially planted species. And it is a secondary forest formatted by sprout originated stand and being on early succession stage as a young stand. So study site was regarded as not suitable for the habitats for wildlife. The number of mammal species at study site was total 22 species, but most of these species were found as not so many in individual numbers. The individual number of hare was approximately 4 to 8, while the raccoon dog and water deer with 2 to 3 and 1 to 2, respectively. As such, it was estimated as a low population density due to discontinuation of ecosystem and the man-made impacts. In this study, raccoon dog and water deer were selected as the target species because of their migration patterns and ecological value in local ecosystem. It was suggested to establish a wildlife corridors of an over-bridge type at the peak area of Galmachi, known to be the mostly-used migratory route for 2 target species.

Epizootiological Study on Infestation Rate of Parasites in Zoo Animals (동물원(動物園)에서 사육중(飼育中)인 야생동물(野生動物)의 기생충감염(寄生蟲感染) 관(關)한 조사(調査))

  • Lim, Young Jae;Lee, Won Chang
    • Korean Journal of Veterinary Research
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    • v.17 no.1
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    • pp.17-26
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    • 1977
  • Parasites of wild animals are closely related with parasites of domestic animals. Wild animals take charge of an important role at parasitic infestation of domestic animals because of unrestrained movement. The authors carried out the work of actual condition of parasitic infestation on wild animals, total 1,014 cases, in the Korean Zoo. The results are summarized as follows: 1. Total rate of parasitic infestation was 36.1% with infestation of 366 among 1,014 cases. The rate of single infestation was 32.6% with infestation of 331 cases, double infestation 3.1% with 31 cases, triple infestation 0.2% with 2 cases and quadrople infestation 0.2% with 2 cases. 2. The parasites on the zoo animals were identified as follows: Lion: Sarcoptiform, Toxocara sp., Toxascaris leonina, Ancylostoma sp. and Isospora spp. Puma: Toxocara sp., Ancylostoma sp. and Isospora sp. Leopard: Toxocara spp., Ancylostoma sp., Trichuris sp., Dibothriocephalus sp. and Physaloptera sp. Wolf: Sarcoptiform and Dibothriocephalus spp. Fox: Trichuris sp., Capillaria aerophila, Spirocerca sp., Paragonimas kellicotti. Jackal: Sarcoptiform, Ascaris sp. and Echinococcus granulosus. Wild Cat: Dibothriocephalus sp. Tiger: Toxascaris leonina. Bear: Sarcoptiform, Metastrongylus apri, Ancylostoma sp. and Ascaris sp. Raccoon and Raccoon dog: Sarcoptiform, Paragonimus kelliotti, and Isospora sp. Boar: Oesophagostomum spp. and Eimeria spp. Mortkey: Sarcoptiform, Trichuris sp., Physaloptera spp.. Enterobius sp. and Isospora sp. Elephant: Sarcoptiform, Strongyloides sp. and Strongylus spp. Deer: Sarcoptiform, Strongyloides sp., Trichuris ovis, Mccistocirrus digitatus, Haemonchus sp., Oesophagostomum radiatum, Paramphistornum spp., Bunostomum phlebotomum, Fasciola hepatica and Eimeria spp. Bison: Sarcoptiform, Haernonchus sp., Marshallagia sp., Nematodirus sp. and Eimeria sp. Zebra: Strongylus sp. and Parascaris equorum. Goral and Barbary: Sarcoptiform, Haemonchus sp., Oesophagostomum venulosum, Moniezia sp. and Eimeria spp. Lama: Strongyloides sp. and Haemonchus sp. Kangaroo: Strongyloides sp. and Haemonchus sp. Camel: Strongyloides sp., Trichuris ovis and Eimeria sp. Peacock and the Other Birds: Sarcoptiform, Capillaria contorta, Capillaria caudinflata, Ascaridia spp., Heterakis spp., Hymenolepis sp., Eimeria spp., Histomonas, Ornithionyssus bacoti, Macrochelidae and Trichomonas. 3. Among the zoo animals, wild carnivora were infestated with the parasites which are common parasites of dogs and cats, wild herbivora were infestated with the parasites of herbivora domestic animals. and wild fowls were infestated with the parasites of domestic fowls.

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Conformational Analysis of Cyclodextrins and Their Methylated Analogs (시클로 덱스티린과 그 메틸유도체의 구조분석)

  • Hee-Sook Choi
    • Journal of the Korean Chemical Society
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    • v.36 no.2
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    • pp.324-328
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    • 1992
  • The $^1H$ NMR chemical shifts and coupling constants for ${\alpha}$-, permethyl-${\alpha}$-, ${\beta}$-and permethyl-${\beta}$-cyclodextrins in neutral aqueous media were assigned based on the 470MHz spectra. In order to obtain accurate chemical shifts and coupling constants the experimental spectra were analyzed with the Raccoon spin simulation program. The rotamer distribution around the$C_{5-}C_6$ bond of the cyclodextrins evaluated from the coupling constants of $J_{56a}$ and $J_{56b}$. In our calculation of the ${\alpha}$-, and ${\beta}$-cycliodextrin showed that gg conformers were most favorable form and tg conformers were least favorable form. It is very interesting to note the changes in $J_{56a}$, $J_{56b}$ coupling constants of permethylated ${\alpha}$- and ${\beta}$-cyclodextrins from unmodified one. The gg conformers were more increased than unmodified one and instead of tg conformers gt conformers were least favorable one upon methylation.

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A DEVELOPMENT OF WOOL FABRICS FOR NAME KOREAN Wool Fabrics From Ancient To Koryo (명각으로 본 모직물의 개건 -고대부터 고려시대까지-)

  • 박순지;이춘계
    • Journal of the Korean Society of Costume
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    • v.21
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    • pp.19-30
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    • 1993
  • There are record that ancient Korean WOOl fabrics male from sheep, camel, rabbit. bear, horse. calile, raccoon dog, fox and so on. Wool fiber fabriher were also reported Kye, Gal, China, Tap Dung, Kuyu anul ram. These wool fabrics wre named amongling to their fineness and weaving method in the fabric. Technology of wool fabric weaving was developed and from Sam Kuk dynasties to to Koryo Dynasty wool fabrics were reported to weave and trade to China, Japan and Arabia. These fabrics were mainly used as clothes, rugs or blankets. In Koryo Dynasty, especially Kye Kurn was used for trading goods to China. There is another record that two thousand sheep, camels offord Koryo by Yo and Kum Dynasty. In this study, the characteristics of Korean wool fabrics will be disscussed from the literature survey of the relevant references.

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