• BMB Reports
• /
• 제34권3호
• /
• pp.250-258
• /
• 2001

For a long time Rhus verniciflua Stokes (RVS) has traditionally been used as a herbal plant. It is known to contain various biological activities. Previously, a crude ethanol extract from RVS was reported to have antioxidant effects, and antiproliferative activities, on human cancer cell lines. In this report, we prepared a highly purified ethanol extract from RVS, which did not contain the urushiol derivatives, named REEE-1 ($\underline{R}$hus $\underline{e}$thanol $\underline{e}$luted $\underline{e}$xtract-1), to investigate the mechanisms of the scavenging activity of hydroxyl radicals using mouse thymocytes. The results from the deoxyribose, DNA nicking, and glucose/glucose oxidase enzyme assays showed that REEE-1 contained a strong scavenging activity of oxygen free radicals, especially of hydroxyl radicals. However, interestingly, REEE-1 also showed cytotoxicity against the thymocytes, although the effect was variable, depending on the concentrations and times of treatment. The REEE-1-mediated cytotoxicity against thymocytes, which has been used as one of the well-characterized models for apoptosis studies, was verified to be apoptotic. This was proven by the following: the appearance of DNA laddering, increases in DNA fragmentation, low fluorescence intensity in the nuclei after propidium iodide staining, and positive Annexin V staining of the cells. These results suggested that REEE-1 had both antioxidative activity and cytotoxicity against the thymocytes, although the effect of the cytotoxicity was variable, depending on the dose and time of the treatment.

• Journal of Microbiology and Biotechnology
• /
• 제28권3호
• /
• pp.465-472
• /
• 2018

Our aim was to determine the detection rate of respiratory viruses (RVs) in feces of patients with acute viral respiratory infection (AVRI) and the detection rate of diarrheal viruses (DVs) in nasopharyngeal samples from patients with acute viral gastroenteritis. The relationships between the presence of fecal RVs or nasopharyngeal DVs and their impacts on the clinical severity were also investigated. A total of 144 fecal specimens were collected from AVRI patients and 95 nasopharyngeal specimens were collected from acute viral gastroenteritis patients. Clinical characteristics and laboratory profiles were compared between subgroups on the basis of the presence or absence of virus in the specimens. The detection rate of RVs in feces was 17.4% (25/144), whereas the detection rate for viruses identical to the respiratory pathogen was 10.4% (identical group, 15/144). Within the identical group, adenovirus (86.7%, 13/15) was most commonly found. Patients in the identical group showed statistically higher values for C-reactive protein, mean age, increased frequency of vomiting, and decreased frequency of chest film involvement and cough (p < 0.05). The detection rate of nasopharyngeal DVs among acute viral gastroenteritis patients was 19.0% (18/95), and in the identical group it was 15.8% (15/95). Norovirus group II and enteric adenovirus were the major pathogens detected in the identical group. There were no significant differences in clinical characteristics and laboratory profiles between the subgroups. In conclusion, the major pathogens of fecal RV and nasopharyngeal DV were adenovirus and norovirus group II, respectively. However, their relationship with the clinical symptoms or disease severity is unclear.

• Journal of Microbiology and Biotechnology
• /
• 제27권6호
• /
• pp.1090-1097
• /
• 2017

Rhus verniciflua Stokes (RVS), an herbal medicine found in East Asia, was extracted and further fractionated to investigate its antioxidant capacity and neuroprotective effects. The RVS ethyl acetate (EtOAc) fraction had the highest level of total phenolics and antioxidant capacity among all solvent fractions tested. Pretreatment of PC-12 cells with the EtOAc fraction effectively attenuated $H_2O_2$-induced oxidative damage. Furthermore, the EtOAc fraction significantly attenuated caspase-3 activity, resulting in inhibition of $H_2O_2$-induced apoptosis. We identified and quantified fustin, sulfuretin, and butein in the EtOAc fraction using accurate mass quadrupole time-of-flight mass spectrometry and reversed-phase high-performance liquid chromatography. The intracellular antioxidant capacity and superoxide dismutase (SOD) activity were significantly increased in PC-12 cells treated with the EtOAc fraction and with individual flavonoids. When cells were pretreated with the EtOAc fraction or individual flavonoids and then co-incubated with diethyldithiocarbamic acid (an inhibitor of SOD activity), cell viability against $H_2O_2$-induced oxidative stress was attenuated. These results suggest that the RVS EtOAc fraction and its flavonoid constituents protect PC-12 cells against $H_2O_2$-induced neurotoxicity through their antioxidant properties.

• Toxicological Research
• /
• 제21권4호
• /
• pp.309-318
• /
• 2005

Rhus verniciflua Stokes (RVS) has been used as a food supplement and a traditional herbal medicine. In this study, we prepared various flavonoid fractions (RS, RW1, RW2 and RWE) from a hot water extract of RVS and their influence on male reproductive organs and spermatogenesis were studied in rats which were orally administered 200 mg/kg of them for 8 weeks. All experimental groups did not show any significant changes in body weight and blood clinical chemistry for liver function. Plasma testosterone level was elevated about 3.7, 5.2 and 6.3 folds in RW1, RW2 and RWE groups, respectively. The weights of testes and epididymides tended to increase slightly without the statistical significance in RW2 and RWE. The spermatozoon motility and epididymal sperm concentration were significantly increased (P<0.05) in RWE and RW1, respectively, when compared to the control group. There was no significant difference in histology and apparent shape of testes and epididymides among the control and the experimental groups. Collectively, RWE showed effectively the elevation of plasma testosterone level, spermatozoon motility and the epididymal sperm concentration without the significant increase of testis and epidiymides weights. When the component HPLC profile among the flavonoids fractions of RVS was compared, the ratio of components were only different. These findings suggest that the Rhus flavonoid fraction, particularly RWE, can stimulate the androgen-dependent male sexual function and it can be applied to the material of functional food for enhancing the sexual function.

• BMB Reports
• /
• 제36권4호
• /
• pp.337-343
• /
• 2003

Rhus verniciflua Stokes (RVS) has been used as a traditional herbal medicine. Several earlier studies indicated that an ethanol extract of RVS has both anti-oxidant and anti-tumor properties, although the mechanism for the activity remains to be elucidated. In this report, we prepared a highly purified ethanol extract from RVS, named REEE-1 ($\underline{R}$hus $\underline{e}$thanol $\underline{e}$luted $\underline{e}$xtract-1), and investigated the mechanism involved in its growth-inhibitory effect on the human B and T lymphoma cell lines, BJAB and Jurkat, respectively. Results from tritium uptake proliferation assays showed that the proliferative capacities of both BJAB and Jurkat cells were strongly suppressed in the presence of REEE-1. This was further confirmed through trypan blue exclusion experiments that revealed a dose-dependent decrease in viable cell numbers after REEE-1 treatment. REEE-1-mediated suppression of cell growth was verified to be apoptotic, based on the increase in DNA fragmentation, low fluorescence intensity in nuclei after propidium iodide staining, and the appearance of DNA laddering. In particular, REEE-1 exerted its anti-oxidant activity through the inhibition of hydroxyl radical-mediated degradation by iron ion chelation rather than direct scavenging of hydroxyl radicals. Furthermore, REEE-1 was revealed to be a potential scavenger of superoxide anions. Collectively, our findings suggest that REEE-1 is a natural anti-oxidant that could be used as a cancer chemo-preventive and therapeutic agent.

• 대한암한의학회지
• /
• 제13권1호
• /
• pp.55-61
• /
• 2008

Nearly 800,000 new colorectal cancer cases are believed to be occur each year, which account for approximately 10% of all incident cancers, and mortality from colorectal cancer is estimated at nearly 450,000 per year. We report one patient with rectal cancer of stage III. The patient had a rectal cancer(StageIII T3N2M0) and underwent low anterior resection in 2004 followed by chemotherapy and radiotherapy. Lymph node metastases were found in 2005 and the patient was treated by palliative chemotherapy, which could not supress the lymph node metastases increase. The patient visited Kyunghee University East-West Neo Medical Center(KHNMC) in 2006 and was treated using allergen-removed Rhus Verniciflua Stokes(aRVS). Radiotherapy was done simultaneously in 2006. There was no evidence of recurrence since then. The patient showed no side effects. This case report brings forth the importance of a thorough study in rectal cancer in the traditional Korean medical point of view, along with the long-term effect of aRVS.

• 대한예방한의학회지
• /
• 제10권1호
• /
• pp.75-93
• /
• 2006

Rhus verniciflua Stokes(RVS) has been widely used as a food and traditional herbal medicine in Korea. RVS has been reported that the extract from its wood and fruit has strong antioxidant activity and anticancer effect but there is little information on Lacca Sinica Exsiccata(LSE), the resin of RVS, as a medicinal use. The aim of this study was to evaluate the antidiabetic effect of ethanol-eluted extract of LSE on streptozotocin(STZ) - induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats with STZ injection. Oral administration of LSE extract(50mg or 100mg/kg of body weight/day) was given to diabetic group. During 4 weeks of experiment, diabetic rats showed significant weight loss and decreasing feed efficiency ratios(FER) compared with normal rats, while the diabetic group orally fed with LSE extract showed a trend of decreasing weight loss and a significant increase of FER(p<0.05). In 4 weeks after induction of diabetes, diabetic rats showed an increase in weight of liver, kidney and heart, whereas the diabetic rats administered with LSE extract showed a reduction in the weight of heart. Blood glucose level was decreased in diabetic rats treated with LSE extract, but it was not statistically significant. Glutamic oxaloacetic transaminase, Glutamic pyruvate transaminase and total cholesterol levels were lower in the diabetic group treated with LSE extract than in untreated diabetic group, but not significant. These results present that LSE may partly have antidiabetic effect and may protect against the development of diabetic heart complications resulting from impaired glucose metabolism.

• 한국어병학회지
• /
• 제9권2호
• /
• pp.177-183
• /
• 1996

이상유영 증상을 나타내는 연어과 어류에서 분리된 새로운 RNA 바이러스의 역전사 효소활성을 조사하였다. 정제바이러스는 래트로바이러스 특유의 유전자전환 효소인 역전사효소 (RTase) 활성을 가지고 있었다. Sucrose 밀도구배 초원심법으로 바이러스 밀도를 조사한 결과 1.16 g/ml에서 밴드를 형성하고, 그 부분의 역전사효소 활성이 최고를 나타내었으며, 전자현미경 관찰결과 외막을 가진 크기 85 nm의 레트로바이러스 형태를 확인할 수 있었다. 인위감염 어류의 뇌 및 신장 조직내에 감염되어있는 바이러스를 대상으로 RTase 활성을 조사한 결과 높은 활성치를 확인할 수 있었다. 정제 바이러스의 구성 단백질은 전기영동법으로 확인한 결과 11종이었다. 이상의 결과, 새롭게 분리된 바이러스는 레트로바이러스의 일종인 것이 확인되었으므로 그 이름을 RVS (Retrovirus of salmonid)로 부를 것을 제안한다.

• 한국음향학회지
• /
• 제20권1호
• /
• pp.68-76
• /
• 2001

유한요소법을 이용하여 압전 수중음향센서의 모델링 및 음향특성을 해석하였다. 압전 수중음향센서의 해석에서 기본적인 압전-탄성 구조물과 유체-구조물의 연성해석을 위한 유한요소 정식화를 하였으며 무한영역의 음향유체를 처리하기 위하여 IWEE (Infinite Wave Envelop Element)를 도입하였다. Tonpilz형 수중음향센서를 수중 산란체로 볼 경우 입사파가 산란체의 표면을 가진할 때 산란체로부터 발생되는 산란파는 IWEE로 인하여 무한 유체영역에서의 산란파의 감소특성을 갖게 되어 무한영역을 유한영역으로 나눈 인위적인 경계에서 반사가 일어나지 않게 되므로 산란파의 음압을 정확히 구할 수 있었다. 또한, 이러한 산란해석을 바탕으로 입사파에 대한 음향센서 내부의 전기적 응답특성인 RVS (Receiving Voltage Signal)를 구하였다. 이러한 일련의 연구 과정들은 소나 시스템을 정확히 해석하고 음향특성을 예측하는데 큰 도움이 될 것이다.

• 생명과학회지
• /
• 제13권6호
• /
• pp.775-781
• /
• 2003

옻나무(Rhus verniciflua Stokes)로부터 제초제인 paraquat(PQ)의 독성 경감기전을 추구할 목적으로 ethylacetate 분획에서 분리한 fustin 및 sulfuretin을 실험동물에 투여하고서 혈액학적 변화 및 간장 중 활성산소에 미치는 영향 검토한 결과 혈액 생화학적 인자 분석을 종합해 보면 옻나무의 메탄을 엑스(250 mg/kg), 에틸아세테이트 엑스(250 mg/kg), fustin(10 mg/kg) 및 sulfuretin(10 mg/kg)을 2주간 각각 경구투여 하고서 PQ를 투여하므로서 PQ의 독성이 유의성 있게 억제되었다. in vivo에서 옻나무의 분획 및 성분의 투여는 혈액생화학적 검사에서 PQ에 의해 유도된 간독성 지표인 s-GPT, s-GOT치, 신장독성지표인 BUN, creatinine치 및 조직손상의 지표인 ALP, MDA치를 억제하였다 폐조직중의 MDA함량, ALP활성 및 collagen함량도 억제하였다. 이로써 옻나무에는 PQ의 폐독성 및 각 장기의 독성을 효과적으로 경감시켜 줄 수 있는 물질이 함유되어 있는 것으로 사료된다.