• BMB Reports
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• 제29권2호
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• pp.99-104
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• 1996

Site-directed substitutions were made to change the Ile91 of restriction endonuclease EcoRV to either Val, Ala or Gly to identify the role of Ile91 in recognition and catalysis, since substitution of Ile91 with Leu afforded dramatic effects on the activity and properties of restriction endonuclease EcoRV. These changes alter the size of the hydrophobic side chain at position 91 and thus might have revealed the reason for the altered phenotype of Ile91Leu. However, the properties of Ile91Val and Ile91Ala mutants were much like wild type EcoRV, in both activity and metal ion preference. Ile91Gly had very little activity with either $Mg^{2+}$ or $Mn^{2+}$ as cofactors. To try to understand the unusual $Mn^{2+}$ profile of the Ile91Leu mutant, two double mutants, Ile91Leu;Asp90Asn and Ile91Leu;Glu45Met were created. Both double mutants were seriously disabled by the second amino acid change. Ile91Leu;Glu45Met had some residual activity in the $Mn^{2+}$ reaction buffer, whereas the Ile91Leu;Asp90Asn displayed no detectable activity.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 추계학술대회 논문집 전력기술부문
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• pp.9-11
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• 2008

A protection current transformer (CT) has been widely used for protection devices. When a fault occurs, a CT should provide the faithful reproduction of the primary fault current. However, a CT may saturates due to the magnitude of fault current, dc component primary time constant and the remanent flux of the iron core, and the secondary current of a CT is distorted. The distorted current can cause mal-operation or the operating time delay of a protection relay. This paper provides a modeling of CT saturation using EMTP-RV. The performance of the proposed CT saturation modeling was investigated under various fault conditions varying the fault distance, fault inception angle, and remanent flux of the iron core. The results indicate that the proposed EMTP-RV modeling can operate correctly, and the reasons for CT saturation are verified by EMTP-RV simulations.

• Journal of Chest Surgery
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• 제7권2호
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• pp.201-208
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• 1974

The purpose of this paper is to present author's experience with 6 cases of ruptured aneurysm of sinus of Valsalva which were treated surgically during last 10 years. Among the 6 cases, 5 were male and one was female. All of them originated from the right coronary sinus and 5 cases were ruptured into the RV while remained one into RA. The diagnosis was obtained in 4 cases by cineangiocardiogram. Clinically, we had difficulties in differential diagnosis with combined cases of VSD with A.I. and had special experience in its differentiation during cardiac catheterization. By simultaneous trans-venous and trans-arterial catheterization, identified two catheter tips in the RV, and pull back tracing obtained aortic pressure directly from RV, and RA from RV pressure which were benefit in confirm ruptured aneurysm of the aortic sinus. Surgical correction was performed by means of direct suture closure or combined Teflon pledget Of patch enforcement graft after aneurysm resection by trans-RA or trans-RV approach. All patients had no history of bacterial endocarditis, syphilis, or tuberculosis and operative findings revealed intact coronary sinus except involved one moreover 3 cases combined with high VSD which uggested congenital in origin although pathologic reports revealed only fibrosis. Post-operative course were uneventful in all cases but one who had bleeding and 2 months to 9 years follow up results were good and spend their usual life in all cases.

• 디자인학연구
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• 제14권4호
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• pp.35-45
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• 2001

The origin of Minivan styled RV dates all the way back to the year 1959 when Volkswagen introduced the Microbus in Germany. But the new category of "Minivan" was made, and first used by Chrysler. This was when Chrysler revealed a new vehicle in 1982 named "Minivan". Minivan was successful in gathering the public attention with it′s multi-purpose characteristic. Ever since, many models were introduced in this category by various auto-makers around the world, which made the Minivan segment very competitive. Various Minivans were also introduced by the Korean auto-makers and these models have been finding their place rapidly in the market owing to their unique characteristics. But the reason for the growth of the Minivan category in Korea was quite different from that of other nations. Through out this thesis, the possibilities of a Minivan that can full fill the needs of a new life style of a family in the basis of various documentary records will be discussed. Ways of making roads, where bigger sized Minivans are continuously increasing, more efficient will also be considered. The main topic of this thesis will be about Minivan styled RV(Recreational vehicle) in relation to the change of Korean life style.

• 천문학회보
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• 제35권2호
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• pp.78.2-78.2
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• 2010

We report the detection of a low-amplitude 380.8-day radial velocity (RV) variations in oscillating K2 III star ${\alpha}$ Ari (HD 12929). We do not found the correlation between RV variations and equivalent widths of chromospheric activity indicators ($H{\alpha}$ and CaII 8662 ${\AA}line$). The bisector analysis shows that bisector velocity span (BVS) and RV variations are not strongly correlated with each other. These result suggest that the RV variations could have been produced either by planetary companion or by the surface spots. If this RV variation is indeed caused by a planetary companion, an orbital solution with a period of P = 381 days, a semi-amplitude of K = 41 m/s, and an eccentricity of e = 0.25 fits the data best. Assuming a possible stellar mass of $M_{\bigstar} = 1.4-5.6 M\odot$, we estimate the minimum mass for the companion of m sini = 1.8-4.5 $M_{Jup}$ with an orbital semi-major axis of 1.2-1.9 AU. If confirmed, our finding gives a support to search for exoplanets around giant stars with multi-periodic oscillations.

• 천문학회보
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• 제42권1호
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• pp.42.4-43
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• 2017

We investigate the long-period radial velocity (RV) variations for M giant HD 18438 and K giant HD 158996 using the high-resolution Bohyunsan Observatory Echelle Spectrograph at the 1.8m telescope of Bohyunsan Optical Astronomy Observatory in Korea. These two target stars are important because HD 18438 is the largest star and HD 158996 is the brightest star for exoplantary system candidate so we can understarnd how evolved stars affect planets by researching these stars. We calculated precise RV measurements of 38 and 24 spectra from November 2010 to January 2017 and June 2010 to January 2017, respectively. We dreived the RV variation period for 719.0 days of HD 18438, 775.6 days for HD 158996. We conclude that the RV variation of HD 158996 is caused by planetary companion which has the mass of 14.7 MJup, semi-major axis of 2.2 AU, and eccentricity of 0.27 assuming the stellar mass of $2.34M{\odot}$. On the other hand, the origin of RV variation of HD 18438 with period of 719.0 days is still uncertain. It might be caused by stellar chromospheric activity or planetary companion, so more observations and tests are required.

• 천문학회보
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• 제36권2호
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• pp.140.2-140.2
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• 2011

We present high-resolution radial velocity (RV) measurements of K2 giant HD 66141 from December 2003 to January 2011 using the fiber-fed Bohyunsan Observatory Echelle Spectrograph (BOES) at Bohyunsan Optical Astronomy Observatory (BOAO). We find that the RV measurements for HD 66141 exhibit a periodic variation of 480 days with a semi-amplitude of 146 m/s. We do not find the correlation between RV variations and a chromospheric activity indicator (H line). The Hipparcos photometry as well as bisector velocity span (BVS) also do not show any obvious correlations with RV variations. Thus, Keplerian motion is the most likely explanation, which suggests that the RV variations arise from an orbital motion. Assuming a possible stellar mass of 1.5 $M{\odot}$, for HD 66141, we obtain a minimum mass for the planetary companion of 7.4 MJup with an orbital semi-major axis of 1.4 AU, and an eccentricity of 0.07. We support that planet occurrence rate around evolved stars is more than 10 % (Dollinger et al. 2009) as well as more massive stars do form significantly more massive planetary companions (Johnson et al. 2007; Lovis & Mayor 2007; Dollinger et al. 2009).

• 천문학회지
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• 제47권2호
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• pp.69-76
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• 2014

We report the detection of an exoplanet candidate in orbit around ${\sigma}$ Persei from a radial velocity (RV) survey. The system exhibits periodic RV variations of $579.8{\pm}2.4$ days. The purpose of the survey is to search for low-amplitude and long-period RV variations in giants and examine the origin of the variations using the fiber-fed Bohyunsan Observatory Echelle Spectrograph installed at the 1.8-m telescope of Bohyunsan Optical Astronomy Observatory in Korea. We present high-accuracy RV measurements of ${\sigma}$ Per made from December 2003 to January 2014. We argue that the RV variations are not related to the surface inhomogeneities but instead a Keplerian motion of the planetary companion is the most likely explanation. Assuming a stellar mass of $2.25{\pm}0.5$ $M_{\odot}$, we obtain a minimum planetary companion mass of $6.5{\pm}1.0$ $M_{Jup}$, with an orbital semi-major axis of $1.8{\pm}0.1$ AU, and an eccentricity of $0.3{\pm}0.1$ around ${\sigma}$ Per.

• Journal of Microbiology and Biotechnology
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• 제31권8호
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• pp.1175-1182
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• 2021

We investigated the effect of bovine lactoferricin (Lfcin-B), a peptide derived from bovine lactoferrin, on activation of intestinal epithelial cells in IEC-6 intestinal cell, and protection against in vivo rotavirus (RV) infection. Treatment with Lfcin-B significantly enhanced the growth of IEC-6 cells and increased their capacity for attachment and spreading in culture plates. Also, Lfcin-B synergistically augmented the binding of IEC-6 cells to laminin, a component of the extracellular matrix (ECM). In the analysis of the intracellular mechanism related to Lfcin-B-induced activation of IEC-6 cells, this peptide upregulated tyrosine-dependent phosphorylation of focal adhesion kinase (FAK) and paxillin, which are intracellular proteins associated with cell adhesion, spreading, and signal transduction during cell activation. An experiment using synthetic peptides with various sequences of amino acids revealed that a sequence of 9 amino acids (FKCRRWQWR) corresponding to 17-25 of the N-terminus of Lfcin-B is responsible for the epithelial cell activation. In an in vivo experiment, treatment with Lfcin-B one day before RV infection effectively prevented RV-induced diarrhea and significantly reduced RV titers in the bowels of infected mice. These results suggest that Lfcin-B plays meaningful roles in the maintenance and repair of intestinal mucosal tissues, as well as in protecting against intestinal infection by RV. Collectively, Lfcin-B is a promising candidate with potential applications in drugs or functional foods beneficial for intestinal health and mucosal immunity.

• Nuclear Engineering and Technology
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• 제53권12호
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• pp.4003-4013
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• 2021

During the long-term cooling (LTC) phase of a loss-of-coolant accident (LOCA) in a pressurized water reactor (PWR), water is supplied from the containment sump to the reactor coolant system (RCS) by the flooded sump water to the Reactor Vessel (RV) through the broken pipes. As part of the technical efforts for resolving GSI-191 [( Reid and Crytzer, May. 2007) 1, consideration is needed for the consequences of debris penetrating the sump screen and propagating downstream into the RV. Injection of debris (fiberglass) into the RV during the LTC recirculation phase needs special attention to assure that reactor core cooling is maintained. The point of concern is the potential for debris to adversely affect the reactor core flow paths or heat transfer [2]. However, all the experiments for proving the coolability of RV have been done with the assumption of the most of debris would be transferred to the RV and the bottom nozzle of the FAs. The purpose of the tests is to quantify the amount of the debris that would be accumulated at the lower plenum and the debris that passes through the FAs since non-conservatism of other researches assumptions that have been used in the past experimental or analytical programs.