• Annals of Laboratory Medicine
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• v.38 no.6
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• pp.563-568
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• 2018

Background: Delamanid, bedaquiline, and linezolid have recently been approved for the treatment of multidrug- and extensively drug-resistant (MDR and XDR, respectively) tuberculosis (TB). To use these drugs effectively, drug susceptibility tests, including rapid molecular techniques, are required for accurate diagnosis and treatment. Furthermore, mutation analyses are needed to assess the potential for resistance. We evaluated the minimum inhibitory concentrations (MICs) of these three anti-TB drugs for Korean MDR and XDR clinical strains and mutations in genes related to resistance to these drugs. Methods: MICs were determined for delamanid, bedaquiline, and linezolid using a microdilution method. The PCR products of drug resistance-related genes from 420 clinical Mycobacterium tuberculosis strains were sequenced and aligned to those of M. tuberculosis H37Rv. Results: The overall MICs for delamanid, bedaquiline, and linezolid ranged from ${\leq}0.025$ to >1.6 mg/L, ${\leq}0.0312$ to >4 mg/L, and ${\leq}0.125$ to 1 mg/L, respectively. Numerous mutations were found in drug-susceptible and -resistant strains. We did not detect specific mutations associated with resistance to bedaquiline and linezolid. However, the Gly81Ser and Gly81Asp mutations were associated with resistance to delamanid. Conclusions: We determined the MICs of three anti-TB drugs for Korean MDR and XDR strains and identified various mutations in resistance-related genes. Further studies are needed to determine the genetic mechanisms underlying resistance to these drugs.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.03a
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• pp.330-339
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• 2008

Ground-based interceptors(GBI) comprise a major element of the strategic defense against hostile targets like Intercontinental Ballistic Missiles(ICBM) and reentry vehicles(RV) dispersed from them. An optimum design of the subsystems is required to increase the performance and reliability of these GBI. Propulsion subsystem design and optimization is the motivation for this effort. This paper describes an effort in which an entire GBI missile system, including a multi-stage solid rocket booster, is considered simultaneously in a Genetic Algorithm(GA) performance optimization process. Single goal, constrained optimization is performed. For specified payload and miss distance, time of flight, the most important component in the optimization process is the booster, for its takeoff weight, time of flight, or a combination of the two. The GBI is assumed to be a multistage missile that uses target location data provided by two ground based RF radar sensors and two low earth orbit(LEO) IR sensors. 3Dimensional model is developed for a multistage target with a boost phase acceleration profile that depends on total mass, propellant mass and the specific impulse in the gravity field. The monostatic radar cross section (RCS) data of a three stage ICBM is used. For preliminary design, GBI is assumed to have a fixed initial position from the target launch point and zero launch delay. GBI carries the Kill Vehicle(KV) to an optimal position in space to allow it to complete the intercept. The objective is to design and optimize the propulsion system for the GBI that will fulfill mission requirements and objectives. The KV weight and volume requirements are specified in the problem definition before the optimization is computed. We have considered only continuous design variables, while considering discrete variables as input. Though the number of stages should also be one of the design variables, however, in this paper it is fixed as three. The elite solution from GA is passed on to(Sequential Quadratic Programming) SQP as near optimal guess. The SQP then performs local convergence to identify the minimum mass of the GBI. The performance of the three staged GBI is validated using a ballistic missile intercept scenario modeled in Matlab/SIMULINK.

• Ocean and Polar Research
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• v.41 no.2
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• pp.63-77
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• 2019

In order to investigate the behavior and seasonal variability of Mn as one of the bio-essential metals in the Amundsen sea, which is known as the most biologically productive coastal area around the Antartica, seawaters were collected using a clean sampling system for 10 stations (96 ea) in 2014 (ANA04B) and for 12 stations (139 ea) in 2016 (ANA06B) surveys of RV ARAON. Dissolved and particulate Mn concentration varied in the range of 0.15-4.43 nmol/kg and <0.01 to 2.42 nM in 2014 and in the range of 0.25-4.15 nmol/kg and 0.01-2.64 nM in 2016, respectively. From the sectional distribution of dissolved and particulate Mn, it might be suggested that dissolved/particulate Mn was provided from iceberg melting and diffusion/resuspension from sediments, respectively. Although this sea is highly productive, there was little evidence regarding the biological origin of dissolved Mn, but particulate Mn only in sea ice and offshore areas could be explained as originating from organic matters, e.g. phytoplanktons. And it could be suggested that the subsurface maximum of dissolved Mn was formed by isopycnal transport of melting materials from ice wall to offshore. Compared to early (2014) summer, temperature, salinity, biomass, dissolved and particulate Mn in late (2016) summer indicated that temporal variations might be resulted from the reduction of ice melting and mCDW flow, which induced a reduction in resuspension. In addition, in the late summer, particles including biomass were reduced, which brought about a reduction in the removal rate of dissolved Mn.

• Journal of ILASS-Korea
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• v.23 no.4
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• pp.185-191
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• 2018

Benzene, toluene, ethylbenzene and xylene (BTEX) were well known as ozone precursors from photochemical reactions and contribute to the formation of photochemical smog which pose health hazards. Also, some of these compounds directly affect the human health due to their toxicity such as benzene. In this study, BTEX ratios and correlation of $C_2$-benzenes (xylenes, ethylbenzene) in vehicle exhaust from recreational vehicle (RV) and multi-purpose vehicle (MPV) were characterized using a chassis dynamometer. VOCs were collected by tedlar bag and a GC/MS system was used for their quantification. Among all of the BTEX, toluene has the highest concentration(more than 30% in composition of BTEX). The average ratio of toluene to benzene emissions (T/B ratio=2.2) was found in vehicle exhaust. The average m,p-xylene/ethylbenzene and m,p-xylene/o-xylene ratios were 1.0 and 3.0 respectively. As a result, it showed a good correlation between the $C_2$-benzenes ($R^2=0.98{\sim}0.99$). In the future, it can be used as a marker for effect evaluation to atmospheric environment by vehicle exhaust.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.1
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• pp.110-117
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• 2019

Objective: An experiment was conducted to evaluate the effects of dietary glutamine (Gln) and arginine (Arg) supplementation on performance, intestinal morphology and ascites mortality in broilers. Methods: A total of 675 day old chicks were randomly allocated to 9 experimental groups in a $3{\times}3$ factorial arrangement based on a completely randomized design with 5 replicates of 15 chicks. Three levels of dietary Gln (0%, 0.5%, and 1%) and Arg (100%, 130%, and 160% of Ross recommendation) supplementation were used in ascites inducing condition ($15^{\circ}C{\pm}1^{\circ}C$) from 7 to 42 days of age. Results: Dietary supplementation of Gln increased body weight gain during grower, finisher and total periods (p<0.05) and increased feed intake during total period. Ascites mortality was decreased by Gln supplementation (p<0.05). Gln supplementation increased the villus height (VH) and crypt depth (CD) in duodenum and jejunum (p<0.05). Arg supplementation decreased CD in duodenum and jejunum, and increased ileum villus width (VW) and also VH/CD ratio in duodenum and jejunum (p<0.05). Both Gln and Arg increased the goblet cell number (GCN) in duodenum whereas Gln supplementation decreased GCN in jejunum and ileum (p<0.05). The $Gln{\times}Arg$ interaction were observed for right ventricle (RV)/total ventricular (TV) ratio, VH, VW, CD, VH/CD. Conclusion: It was concluded that dietary 0.5% Gln alone or along with 130% Arg of Ross requirement, improve the intestinal morphology and performance and hence decrease the ascites mortality in broiler chickens with cold induced ascites.

• Mycobiology
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• v.47 no.2
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• pp.256-260
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• 2019

Neuraminidase (NA) cleaves the glycosidic bond linkages of sialic acids to release the mature virions from infected cells and has been an attractive therapeutic target for anti-influenza agents. In our ongoing investigation of NA inhibitors in mushroom extracts, we found that the extract the fruiting body of Glaziella splendens potently inhibited neuraminidase. The fruiting bodies of G. splendens were extracted and partitioned successively with hexane, ethyl acetate, and butanol. The ethyl acetate soluble-layer was subjected to silica gel and Sephadex LH-20 column chromatographies, and MPLC to obtain five compounds (1-5). Their structures were determined by spectroscopic methods. NA inhibitory activity of these compounds was evaluated using NAs from recombinant rvH1N1, H3N2, and H5N1 influenza A viruses. One compound (1) was elucidated as a new azaphilone derivative, and four compounds (2-5) were identified as entonaemin A, comazaphilone D, rubiginosin A, and entonaemin B, respectively. Compounds 3 and 4 showed considerable inhibitory activity against three types of neuraminidases with the $IC_{50}$ values of 30.9, 41.8, and $35.7{\mu}M$ for 3 and 46.5, 50.4, and $29.9{\mu}M$ for 4, respectively. This study reveals that the fruiting bodies of G. splendens possess azaphilone derivatives with the NA inhibitory activity. This is the first report on the isolation of neuraminidase inhibitors from the fruiting bodies of G. splendens.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.86-86
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• 2019

We present BV photometric observations and high-resolution spectra of AO Ser, which were obtained at the Mt. Lemmon Optical Astronomy Observatory (LOAO) and the Bohyunsan Optical Astronomy Observatory (BOAO), respectively, in 2017. The radial velocities (RVs) for both components were measured, and the effective temperature of the primary star was found to be $T_{eff,1}=8,820{\pm}62K$ by a comparison of the observed spectra and the Kurucz models. A unique set of fundamental parameters of AO Ser were derived for the first time by a simultaneous analysis of the light and RV curves. The results indicate that our program target is a semi-detached eclipsing system with values of $M_1=2.06{\pm}0.11M_{\odot}$ and $M_2=0.41{\pm}0.03M_{\odot}$, $R_1=1.54{\pm}0.03R_{\odot}$ and $R_2=1.30{\pm}0.02R_{\odot}$, and $L_1=12.9{\pm}0.2L_{\odot}$ and $L_2=0.9{\pm}0.3L_{\odot}$. We applied multiple frequency analyses to the eclipse-subtracted light residuals. As a result, two frequencies of $f_1=21.85151days^{-1}$ and $f_2=23.48405days^{-1}$ were detected and their pulsation constants were calculated to $Q_1=0.0344days$ and $Q_2=0.0320days$. The pulsational characteristics and the position in the HR diagram demonstrate that the primary star is a ${\delta}$ Sct pulsator.

• Journal of Coastal Disaster Prevention
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• v.4 no.3
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• pp.111-118
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• 2017

In this study, pull box members were designed by finite element analysis of a steel plate covering a pull box to secure its safety on the landing pier dedicated to the large research survey ship. It was assumed that the maximum load is due to the 250 tonf class crane used for unloading work when the working environment in the upper part of the landing pier was considered. The safety of the pull box was evaluated by the comparison between the yield strength of the steel plate and the result of stress analysis on the steel plate due to the crane load. It was found that the stress at the plate from the crane load exceeded the yield strength of the steel(205MPa) when the upper part of the pull box was protected by a $1950{\times}1950mm$ steel plate cover. In order to compensate for this, a concrete filled steel tube(CFT) column with a diameter of 150 mm and a steel thickness of 10 mm was reinforced at the center of the plate, and the finite element analysis was carried out. However, the maximum stress at the steel plate was higher than the yield strength of the steel in some load cases so that it was tried to find appropriate thickness of the steel plate and diameter of the CFT columns. Finally, the analysis results showed that the safety of the pull box was secured when the thickness of the steel plate and the diameter of the CFT column were increased to 30mm and 180mm, respectively.

• Restorative Dentistry and Endodontics
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• v.46 no.4
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• pp.51.1-51.13
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• 2021

Objectives: This study aimed to evaluate the effect of improper positioning single-peak and multi-peak lights on color change, microhardness of bottom and top, and surface topography of bulk fill and incremental composites after artificial aging for 1 year. Materials and Methods: Bulk fill and incremental composites were cured using multi-peak and single-peak light-emitting diode (LED) following 4 clinical conditions: (1) optimal condition (no angulation or tip displacement), (2) tip-displacement (2 mm), (3) slight tip angulation (α = 20°) and (4) moderate tip angulation (α = 35°). After 1-year of water aging, the specimens were analyzed for color changes (ΔE), Vickers hardness, surface topography (Ra, Rt, and Rv), and scanning electron microscopy. Results: For samples cured by single-peak LED, the improper positioning significantly increases the color change compared to the optimal position regardless of the type of composite (p < 0.001). For multi-peak LED, the type of resin composite and the curing condition displayed a significant effect on ΔE (p < 0.001). For both LEDs, the Vickers hardness and bottom/top ratio of Vickers hardness were affected by the type of composite and the curing condition (p < 0.01). Conclusions: The bulk fill composite presented greater resistance to wear, higher color stability, and better microhardness than the incremental composite when subjected to improper curing. The multi-peak LED improves curing under improper conditions compared to single-peak LED. Prevention of errors when curing composites requires the attention of all personnel involved in the patient's care once the clinical relevance of the appropriate polymerization reflects on reliable long-term outcomes.

• Fisheries and Aquatic Sciences
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• v.26 no.10
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• pp.628-636
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• 2023

Stomach contents of three commercially important species, anchovy (Engraulis japonica), small yellow croaker (Larimichthys polyactis) and yellow goosefish (Lophius litulon) were analyzed to investigate difference of prey between Spring and Fall. Trawl surveys for target fishes were conducted at 12 stations by the RV Tamgu-8 in the Yellow Sea-Korean side in Spring and Fall 2008 as a part of the United Nations Development Programme/Global Environment Facility (UNDP/GEF), Yellow Sea Large Marine Ecosystem (YSLME) survey. Stomach contents of 50 individuals of each species were analyzed to species level of prey, if the number of specimens was more than 50 for each species. Fullness and digestion condition of stomach contents were determined by five and six levels, respectively. In anchovy stomachs, 23 species in Spring and 15 species in Fall were identified, respectively. Stomach contents were mostly occupied by copepods and euphausiids, mostly Euphausia spp., calyptopis in Spring while by copepods and amphipods in Fall. In small yellow croaker stomachs, 23 species in Spring and 11 species in Fall were identified. Stomach contents were mostly occupied by copepods and euphausiids in Spring, but by only euphausiids in Fall. Total 368 yellow goosefish (151 in Spring and 217 in Fall) were captured, but stomach contents only in Fall were analyzed. Most of stomach contents were anchovy with small proportion of Hakodate sand shrimp, Tanaka's snailfish, Pacific cod, and miscellaneous things. The present research unveiled that main food items of plankton feeder were distinctly different by species and seasons in the Yellow Sea-Korean side, as coincided with previous reports.