• 제목/요약/키워드: RSP

검색결과 128건 처리시간 0.023초

개 정소상체 정자의 난자내 침입율과 동결융해 후의 생존성에 관한 연구 (Studies on the Number of Sperm Penetrated Oocytes and Survival Rate of Frozen-thawed Epididymal Dog Sperm)

  • 박종민;김상근
    • 한국가축번식학회지
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    • 제26권3호
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    • pp.229-234
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    • 2002
  • 본 연구는 불임견의 번식장애를 해결할 목적으로 개 정소상체로부터 채취한 정액을 tris-buffer로 원심분리하여 정장을 제거한 RSP-T 희석 정자의 일반성상과 동결방법 및 glycerol 농도가 동결융해 후 생존성에 미치는 영향과 난자와 정소상체 정자를 매정시켰을 때 정자침입율을 조사하기 위하여 수행하였다. 1. WES, RSP-S 및 RSP-T 희석 정소상체 정액의 정자수는 각각 4.25 $\pm$ 0.25(X$10^{6}$ Cells/$m\ell$), 3.85 $\pm$ 0.20(x$10^{6}$cells/$m\ell$), 4.05 $\pm$ 0.28(X$10^{6}$cells/$m\ell$)이었고, 활력은 50.55 $\pm$ 2.75%, 67.25 $\pm$2.55%, 78.75$\pm$3.55%이었고, 기형정자율은 49.45$\pm$2.25%, 37.75 $\pm$ 2.10%, 24.25 $\pm$ 1.55%이었다. 2. RSP-S와 RSP-T 희석 정소상체 정액을 완만 동결을 했을 때 생존율은 각각 35.00 $\pm$2.35%, 45.50 $\pm$2.15%와 초급속동결시의 생존율은 16.50 $\pm$ 3.55%, 22.55 $\pm$ 3.95%이었다. RSP-T 희석 정소상체 정액을 동결시 내동제에 glycerol 농도를 2.0~8.0% 첨가하여 동결했을 때 생존율은 각각 9.25 $\pm$ 1.55%~17.50$\pm$2.50%로서 신선정액의 25.50% 4.50%~34.00$\pm$5.15%에 비해 낮은 생존율을 나타냈다. 3. 동결 융해한 정소상체 희석정자의 평균 수정 능 획득 율과 첨체반응 및 생존정자수는 각각 13.00 $\pm$ 2.35%, 3.55 $\pm$ 0.85%, 15.50 $\pm$ 1.90% 였고, 신선 정소상체 정액의 45.25$\pm$5.75%, 7.06 $\pm$0.25%, 49.20$\pm$6.80%이었다. 신선 및 동결 수정 능 획득 정자를 난자와 매정시켰을 때 난자내 정자의 침입율은 각각 39.25 $\pm$4.72 %와 34.24$\pm$3.93%였고, 난자당 정자의 침입 수는 각각 1.30$\pm$0.33개, 1.10$\pm$0.50개이었다.

천연보존제로서 한국산 무 및 종자 단백질의 생화학적 특성 : 항유전독성 및 세포독성에 미치는 영향 (The Biochemical Properties of Korean radish (Raphanus sativus L.) and Its Seed Protein as a Natural Preservative : The Influences on Antigenotoxicity and Cytotoxicity)

  • 최윤혁;황철원
    • 생명과학회지
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    • 제15권4호
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    • pp.561-565
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    • 2005
  • 본 연구는 한국산 무 및 그 종자단백질을 추출 및 분리하여 3T3 세포주에 대해 항유전독성과 세포독성에 미치는 영향과 항균활성을 검토하였다. 3T3 세포주에 직접발암원인 $100{\mu}g/ml$ MNNG를 $10{\mu}l$씩 투여한 후 10mg/ml의 무추출물(RJ)과 1mg/ml의 종자단백질(RSP)을 $10{\mu}l$씩 투여하였을 때, 각각 $43\%$$30\%$의 항유전독성을 보였다. 또한 lactose dehydrogenase (LDH) 정량 분석을 이용하여, 3T3 세포주에 대한 다양한 농도의 RJ 및 RSP를 투여한 후 세포독성을 관찰 한 결과 세포독성은 관찰되지 않았다. 한편 이미 오염된 화장품에 대해 10 mg/ml 의 RJ와 1mg/ml의 RSP를 투여하였을 때, 세균성오염화장품 시료(C1)에 대해서 RSP가 $85\%$의 항균활성을 보였으며 진균성오염화장품 시료(C4)에 대해서 RSP는 $79\%$, RJ는 $76\%$의 항균활성을 보였다.

Ethanolic extract of Red Sweet Pepper (Capsicum annuum L.) regulates the skin inflammation in vitro and in vivo

  • Jin, Yu-Mi;Kim, Seong-Sun;Song, Young-Jae;AYE, AYE;Park, Bog-Im;Soh, Ju-Ryun;Jeon, Yong-Deok;Jin, Jong-Sik
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 춘계학술대회
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    • pp.120-120
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    • 2019
  • Allergic inflammatory disease has been increased by abnormal lifestyle and food habits. Especially, prevalence of atopic dermatitis (AD) has been elevated and treatment of AD has not been unclear. Red sweet pepper (RSP), named as Capsicum annuum L, has been known as having pharmacological effects such as antioxidant, detoxification and antibacterial effects. However, the beneficial effect of ethanolic extract of RSP on AD has not been partly examined yet. Therefore, the aim of this study was to investigate anti-inflammatory effects of RSP on AD in vitro and in vivo models. The treatment of RSP inhibited the secretion of inflammatory cytokine such as interleukin (IL)-6 and IL-8 in tumor necrosis factor (TNF)-${\alpha}$ and interferon (IFN)-${\gamma}$-stimulated human keratinocyte (HaCaT cell). Also, RSP extract regulated 2,4-dinitroflorobenzene (DNFB)-induced AD-like skin lesions in BALB/c mice. Oral administration of RSP ameliorated DNFB-induced AD-like symptoms. In presented results indicated that RSP inhibited inflammatory cytokines in HaCaT cell and ameliorated AD-like skin lesion through suppression of symptom of DNFB-induced skin inflammation. Thus, RSP might be a potential therapeutic agent for AD.

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Mechanistic Significances of the Reactivity-Selectivity Principle

  • 이익준;이본수;고한중;장병두
    • Bulletin of the Korean Chemical Society
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    • 제16권3호
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    • pp.277-281
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    • 1995
  • The relationship between the signs of ${\rho}i(0)$, ${\rho}j(0)$ and ${\rho}ij$ and validity of the reactivity-selectivity principle (RSP) has been derived: RSP is valid when W = ${\rho}i(0){\cdot}{\rho}j(0)/{\rho}ij$ is negative. The analysis of 100 reaction series indicated that for normal SN2 reactions involving variations of substituents in the nucleophile (X) and in the substrate (Y) RSP is valid only for a dissociative type for which ${\rho}Y(0)$ is negative, whereas for the acyl transfer reactions with rate-limiting breakdown of the tetrahedral intermediate RSP is valid in general for all substituent changes, X, Y and/or Z (substituent on the leaving group). The trends in the validity of RSP for certain types of reaction can be useful in supplementing the mechanistic criteria based on the signs of ${\rho}i(0)$, ${\rho}j(0)$ and ${\rho}ij$.

전자선화증권의 운용실증실험상의 문제점과 해결방안 (The Problems and Solutions in the Pilot Test of Electronic Bill of Lading)

  • 최석범
    • 통상정보연구
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    • 제6권3호
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    • pp.321-349
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    • 2004
  • Bolero Project initially had the support of TEDIS, and now is a joint-venture between SWIFT and IT Club. After the successful testing of an original pilot project, The Bolero Association was formed in 1995 by a group of interested cross industry companies. A Bolero Bill of Lading replicates the basic functions of a paper-based bill of lading via title registry service and core messaging platform. A Bolero Bill of Lading consists of BBL Text and Title Registry Record. A Tittle Registry Record carries out the function of a document of title and a BBL may be transferred by changing the roles of users in this Title Registry Record. TEDI Project had been conducted from the April 1999 to the September 2000 on the basis of the EDEN Project(Dec.97- Mar. 1999) and International Trade Guidances Project (Nov. 1998-Mar. 1999). Through TEDI Project, the RSP Model was introduced as e-trade solution like Bolero's Solution. The RSP Model Solution will be furnished through TC (Trade Chain) Server and RSP(Repository Service Provider) Server. The purpose of this paper is to study the problems and solutions in the pilot test of electronic Bill of Lading using TEDI's RSP and to promote the introduction of electronic Bill of Lading.

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전자선화증권의 기능에 대한 사례분석과 시사점 (Implications in Case Study on the Electronic Bills of Lading)

  • 최석범;김태환
    • 통상정보연구
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    • 제7권1호
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    • pp.171-194
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    • 2005
  • The CMI Rules for Electronic Bills of Lading were drafted by the Comite Maritime International(CMI) and related specifically to EDI transfers of negotiable bills of lading. The CMI Rules are contractual in nature and the application of the CMI Rules is strictly voluntary. The method adopted in the CMI Rules to enable negotiation of the rights to cargo in a controlled method is based on the introduction of the ‘Private Key’. The Bolero service will be governed by a multilateral contract called the Bolero Rule Book which specifies the rights and responsibilities of Bolero and its users. The Title Registry and Bolero Bill of Lading provide a fully functional equivalent to the paper bill of lading. The Bolero Bill of Lading can be created, transferred, amended, and surrendered by way of designating to order party, blank endorsement, refusal by the transferee etc. In Japan, through TEDI Project, the RSP Model is introduced as e-trade solution like Bolero's Solution. The RSP Model Solution will be furnished through TC(Trade Chain) Server and RSP(Repository Service Provider) Server. The purpose of this paper is to analyze comparatively the CMI Rules and Bolero Bill of Lading and RSP Model of TEDI and to find the implications in this case study for quick introduction of electronic Bill of Lading.

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분산 OSGi 프레임워크들로 구성된 홈 네트워크에서 P2P을 이용한 RSP 번들 설계 및 구현 (A Design and Implementation of P2P based RSP bundle for Home Network of Distributed OSGi Frameworks)

  • 윤기현;김은회;최재영
    • 한국정보과학회:학술대회논문집
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    • 한국정보과학회 2008년도 한국컴퓨터종합학술대회논문집 Vol.35 No.1 (D)
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    • pp.412-417
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    • 2008
  • 현재 OSGi 프레임워크의 서비스는 로컬 장비에서만 공유할 수 있기 때문에 하나의 장비에 서비스 부하가 집중되는 문제점을 가진다 이와 같은 문제점을 해결하기 위해 확장 서비스 레지스트리를 이용하는 방법이 제안되었지만, 확장 서비스 레지스트리를 재 검색해야 하므로 응답시간이 늦어지는 단점을 가진다. 따라서 본 논문에서는 추가적인 확장 서비스 레지스트리를 재 검색할 필요가 없고, 단지 로컬 서비스 레지스트리만을 검색하여 원격 서비스를 실행할 수 있는 P2P 기반의 RSP 번들을 설계하고 아기 보호를 위한 유비쿼터스 홈 시스템에 실제로 적용하여 구현하였다. 그러므로 본 논문에서 제안한 RSP 번들을 이용하면 하나의 OSGi 프레임워크에 집중될 수 있는 부하를 분산시킬 수 있고, 기존에 개발된 서비스들도 원격 서비스로 재정의하여 다른 OSGi 프레임워크와 서비스를 공유할 수 있게 된다.

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복싱 국가대표선수 라이트 스트레이트 펀치 동작의 Kinematic 특성분석-사례연구 (The Case Study of A Kinematic Analysis of the Right-Straight Punch in Korean National Representative Boxers)

  • 김의환;김진표;이진욱
    • 한국운동역학회지
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    • 제13권3호
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    • pp.293-309
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    • 2003
  • The purpose of this study was to analyze the kinematic variables of the right-straight punch(RSP) in boxing with three-dimensional analysis technical methods. The subjects are boxers who have been playing in national boxing representative team and the RSP is their special favorite technique, The right-straight punches were filmed on 16mm video cameras(30frames/sec.) The kinematic variables were temporal, postural and center of gravity(COG). The mean and the standard deviation of variables have been obtained and used as basic factors for examining characteristics of the RSP by out-boxers. From the data analysis and discussion, the following conclusions have been drawn. 1) Temporal variables It is a significant characteristic that LDJ and KDM s' the amount of elapsed time(EF) needed for both an attack and a defense were similar : ET for stretch-out of attack-arm was $0.52{\pm}0.04\;sec$. and return was $0.54{\pm}0.01\;sec$. Therefore, a defense motion is as important as an attack motion. 2) Posture variables When the subjects performed a RSP, the significant characteristic of the ankle angle was that it wasn't completely returned to the original position after stretching-out. Therefore it is necessary to do supplementary exercises, such as side steps, to move the center of gravity more effectively. The hee angle was not fully stretched either. In regard to the hip angle, it should be rotated with all strength to harmonize with the direction of movement. 3) Center of Gravity(COG) variables When both LDJ and KDM performed a RSP, a significant characteristic was the transformation of sagittal view rather than transverse or frontal views.

편측 견부 통증 환자의 안정시 견갑골 자세와 통증수준과의 상관성 연구 (The Relationship Between Resting Scapular Position and Pain Level in Unilateral Shoulder Pain)

  • 정영민;최종덕
    • 한국전문물리치료학회지
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    • 제17권2호
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    • pp.25-32
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    • 2010
  • The purpose of this study was to investigate the most effective and comprehensible method for the assessment of resting scapular position (RSP) and pain level (PL) in unilateral shoulder pain (USP). Fifty volunteers with USP were involved in the study. Resting scapular assessments of the patients' pain sides (PS) and non-pain sides (NPS) were evaluated. The assessment tools for RSP are: 1) sternal notch (SN) to coracoid process (CP) distance 2) 3rd thoracic spinous process (T3S) to posterolateral angle of acromion (PLA) distance 3) scapular index 4) 8th thoracic spinous process (T8S) to inferior angle of scapular (IAS) distance 5) supine measurement of pectoralis minor (PM) distance 6) standing PM distance 7) PM index (PMI) and 8) PM pain. The paired t-test was used to compare PS and NPS in RSP. Pearson correlation analysis was used to confer a relationship between the PL and RSP. The results of this study indicated that: 1) all the variables between the PS and NPS for RSP were statistically significant(p<.05) and 2) the PMI showed the strongest relationship in the correlation analysis between RSP and PL(p<.05, r=.37). Therefore, it can be concluded that there is a relationship between PMI and PL and it is suggested that an assessment tool using PMI to diagnose shoulder pain would be clinically effective.