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Metabolic Engineering of Rational Screened Saccharopolyspora spinosa for the Enhancement of Spinosyns A and D Production

  • Jha, Amit Kumar;Pokhrel, Anaya Raj;Chaudhary, Amit Kumar;Park, Seong-Whan;Cho, Wan Je;Sohng, Jae Kyung
    • Molecules and Cells
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    • v.37 no.10
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    • pp.727-733
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    • 2014
  • Spinosyns A and D are potent ingredient for insect control with exceptional safety to non-target organisms. It consists of a 21-carbon tetracyclic lactone with forosamine and tri-Omethylated rhamnose which are derived from S-adenosyl-methionine. Although previous studies have revealed the involvement of metK1 (S-adenosylmethionine synthetase), rmbA (glucose-1-phosphate thymidylyltransferase), and rmbB (TDP-D-glucose-4, 6-dehydratase) in the biosynthesis of spinosad, expression of these genes into rational screened Saccharopolyspora spinosa (S. spinosa MUV) has not been elucidated till date. In the present study, S. spinosa MUV was developed to utilize for metabolic engineering. The yield of spinosyns A and D in S. spinosa MUV was $244mgL^{-1}$ and $129mgL^{-1}$, which was 4.88-fold and 4.77-fold higher than that in the wild-type ($50mgL^{-1}$ and $27mgL^{-1}$), respectively. To achieve the better production; positive regulator metK1-sp, rmbA and rmbB genes from Streptomyces peucetius, were expressed and co-expressed in S. spinosa MUV under the control of strong $ermE^*$ promoter, using an integration vector pSET152 and expression vector pIBR25, respectively. Here-with, the genetically engineered strain of S. spinosa MUV, produce spinosyns A and D up to $372/217mgL^{-1}$ that is 7.44/8.03-fold greater than that of wild type. This result demonstrates the use of metabolic engineering on rationally developed high producing natural variants for the production.

Analysis of Fuel Marginal Price for Biomass Power Plant - On the Basis of China Biomass Power Plant - (바이오매스 발전소 연료한계단가 분석 - 중국 바이오매스 발전소를 중심으로 -)

  • Kim, Cheol;Sa, Jae-Hwan;Kim, YunSoung;Jeon, Eui-Chan
    • Journal of Climate Change Research
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    • v.1 no.3
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    • pp.219-226
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    • 2010
  • This study is analyzed the financial feasibility of biomass power plant in China in terms of fuel marginal price of biomass power plant. The range of fuel price is 150~300 RMB and IRR(Internal Rate of Return), NPV(Net Present Value), DSCR(Debt Service Coverage Ratio) and operation time are analyzed by 10 RMB from 150 RMB. The sensitivity of IRR went down by 1.35 on average. The sensibility of NPV showed big difference by 20% on 260 RMB and 270 RMB. In addition, DSCR of loan is at 1.0 at raw cost of 242 RMB and at lower than 1.0 when the raw cost over 242 RMB. It means that the pay-off of principal and interest of the loan is expected to be difficult in that case. The operation time of power plant should be 88% against standard operation time to maintain over 1.0 of DSCR. Therefore, the factors affecting the cost of raw material to build the power plant and to operate it should be prioritized.

A Study on the Online Arbitration Rules in China (중국 온라인중재규칙에 관한 연구)

  • Choi, Seok-Beom
    • Journal of Arbitration Studies
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    • v.21 no.2
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    • pp.47-64
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    • 2011
  • The China International Economic and Trade Arbitration Commission(CIETAC) released online arbitration rules which apply the resolution of disputes over electronic commerce transactions, as well as other economic and trade disputes in which the parties agree to do. The evidence submitted by the parties may be electronic evidence created, sent, received or stored by electronic, optical or magnetic means. Electronic evidence with a reliable electronic signature shall carry the same effect and probative force as a document with a hand-written signature. Where a case is tried in a tribunal, the arbitration tribunal shall conduct an online trial hearing using internet video conference or other electronic or computer communication means. Unless the parties have another agreement, summary procedure shall apply to cases where the amount in dispute exceeds RMB 100,000 but no more than RMB 1 million, or where the amount in dispute exceeds RMB 1 million and a party submits a written application for summary procedure after obtaining the written consent of the other party. Unless the parties have agreed otherwise, fast-track procedure shall apply to cases where the amount in dispute does not exceed RMB 100,000 or where the amount in dispute exceeds RMB 100,000 and a party submits a written application for fast-track procedure after obtaining the written consent of the other party. Notable features of the Online Rules are as follows; first, there is not detailed consideration for online arbitration. Second, communications between the parties and the tribunal are allowed only through the Secretariat. Third, elaborate provisions regarding the electronic submission and transmission of documents is provided for. Forth, various factors must be considered by the tribunal in deciding the evidence's reliability. Fifth, reasonable endeavours is levied on CIETAC to keep data communications secure and encrypted. Sixth, the tribunal has the right to investigate and collect relevant evidence. And finally different procedures are provided for in consideration of the various types of E-commerce.

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Income Analysis of Orange Farming in China (중국 밀감재배의 소득분석)

  • Kim, Jai-Hong;Pan, Li
    • Korean Journal of Agricultural Science
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    • v.36 no.2
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    • pp.233-244
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    • 2009
  • This study analyzed the income of orange farming of Lichuan, Jiangxi Province in China. Using a questionnaire, 36 farmhouseholds of Lichuan were surveyed. The results are as follows. First, through the investigation of Lichuan orange farming farmhouseholds, we know that the direct expenses of Lichuan orange is around 2,250 RMB/10a and the direct expenses per household's are about 32,000 RMB. The average production of Lichuan orange is 1,548 kg/10a and the income is around 2,200 RMB/10a. The results showed that in Lichuan area orange farming benefit is more than rice so orange farming has been increasing. Second, the direct expense of Lichuan orange farming, 91% is material cost, 8% is labor cost, depreciation cost is only 1.4%. This result show that orange farming in Lichuan is very labor intensive. Third, there are a lots of young orange trees in Lichuan, so income would be increasing through the year.

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The Regionalization of the RMB in Southeast Asia: Coupling or Decoupling of Local Currency/Dollar Exchange Rates with the RMB/Dollar Exchange Rate (동남아시아에서의 위안화 국제화: 위안화 환율에 대한 개별국가 환율의 동조화 또는 비동조화 현상을 중심으로)

  • RA, Hee-Ryang
    • The Southeast Asian review
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    • v.23 no.1
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    • pp.313-362
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    • 2013
  • 중국의 위안화 국제화(지역화)는 중국경제의 성장 및 중국정부의 전략적, 정책적 지원에 따라 가속화되고 있다. 특히 최근 ASEAN과 중국 간 경제통합이 빠르게 진행됨에 따라 동남아시아 지역에서 중국 위안화의 유통이 확대되고 있다. 본 논문은 이러한 위안화 유통의 확대와 관련하여 위안화 국제화(지역화)가 동남아시아 국가들의 환율정책에 미치는 영향을 분석하고자 한다. 동 지역에서 위안화의 유통의 확대(위안화 국제화)가 유의미하다면 달러화 대비 위안화 환율과 달러화 대비 동남아시아 개별국가통화 환율 간에 인과적 관계를 보일 것이다. 왜냐하면 환율정책의 중요한 목적 중 하나는 환율의 안정적 운영인데 환율결정에 있어 위안화의 비중이 크다면 그 만큼 달러화 대비 개별국가통화 간 환율의 영향도 커지기 때문이다. 본 논문은 이러한 가설을 바탕으로 두 환율변수 간 공적분 분석 등 계량분석을 통해 가설검정을 실시하였다. 분석 결과 2008년 글로벌 금융위기 이전(2005.8~2008.6)에는 두 환율변수 간 동조화 현상이 나타나는데 비해 그 이후(2010.7~2012.6)는 비동조화 현상이 나타나는 것으로 나타났다. 이는 2010년 이후 유럽의 재정 위기 등 글로벌 경기침체로 인해 동남아시아 국가들의 환율 정책 우선순위가 환율의 안정적 운영에서 경기회복을 위한 수출증가 및 이를 위한 개별국가 통화의 환율절하로 전환하였음을 의미한다고 할 수 있다. 또한 중국과의 국경무역 등 경제적 영향이 상대적으로 큰 GMS(라오스, 미얀마, 베트남)국가들의 경우 그 외 아세안 7개국들에 비해 두 환율변수 간 동조화 현상이 강하게 나타나는 것으로 분석되었다. 이는 이들 국가들이 상대적으로 기타 국가들에 비해 위안화 국제화에 대한 민감도가 높다는 것을 의미한다. 향후 동남아시아 국가들의 경기가 회복되고 위안화의 국제화가 가속되면 두 환율 간 동조화 기조는 강화될 것으로 예상된다.

The China's Exchange Rate Policy to Export Competition

  • Lee, Dong-Hae;Lee, Sang-Ki
    • The Journal of Industrial Distribution & Business
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    • v.8 no.2
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    • pp.5-10
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    • 2017
  • Purpose - The purpose of this paper was to analyze the Chinese government's announcement of the RMB's appreciation on July 1, 2010, and its aim was to ascertain whether the appreciation has affected Chinese export prices by empirically measuring the degree of the exchange rate pass-tough on those prices. Research design, data, and methodology - Using 73 HS trade categories with cross-industry and time-series data, the panel estimation of a fixed-effects model has been applied to measure the degree and stability of any exchange rate pass-through effects. The estimation results show that the export prices of most trade categories were affected by the exchange rate changes. The pass-through effect was generally small, at about -0.485, and statistically significant in most export prices. Results - The empirical results indicate that China would lose its advantage and competitiveness in export if the RMB were appreciated continuously and rapidly because its export goods would no longer operate under strong monopolistic competition. Conclusions - The implications for China's exchange rate policy suggest that it would be better for the RMB to appreciate slowly and gradually rather than radically. It is clear that it would be allow the capital free flow in Chinese overall economic interest to reduce the continuous appreciation pressure on the currency and pave the way for improvements in export distribution competitiveness.

울산광산 내 비소로 오염된 광미의 자연저감 능력에 대한 pH와 산화-환원 전위 영향

  • Park Maeng-Eon;Seong Gyu-Yeol;Lee Pyeong-Gu;Kim Pil-Geun
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2005.04a
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    • pp.182-185
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    • 2005
  • 울산광산 내 지표수와 토양 중의 공극수에 함유되어 있는 비소의 오염현황을 파악하고, pH와 산화-환원 전위 값의 변화에 따른 자연저감 능력을 평가하였다. 유비철석을 비롯한 비소함유 광물은 높은 산화-환원 전위 값과 낮은 pH 조건에서 해리되며, 이후 지하수의 진화과정에서 pH가 상승함에 따라 주로 5가의 비소형태로 존재하게 된다. 울산광산지역 지하수의 비소농도는 Eh가 높은 비포화대와 포화대 지하수의 경계부에서 높은 경향을 나타내며, 포화대의 상부에서는 Eh가 비교적 일정하나 비소 농도는 다양한 분포양상을 보인다. 포화대 하부에서 비소의 함량은 매우 낮으며, Eh 감소에 따라 비소 함량이 비례적으로 감소한다. 반응경로 과정에서 비소농도는 Eh<-0.1(V)인 지하수 포화대에서 가장 낮으며, pH가 상대적으로 낮고 산화-환원 전위값이 높은 비포화대에서 증가되는 경향을 보인다. 풍화 반응 정도가 높은 광미와 토양에서 비소농도 높으나, 용출실험에서 비소가 기준치 이하로 용출되는 것은 풍화반응과 토양에 의한 비소의 자연저감이 진행되고 있음을 반영한다. RMB를 이용한 중금속 제거능력 평가 실내실험에서, 산성과 알칼리 조건 모두에서 제거율이 높은 것으로 나타났다. 인회석과 철산화물질로 구성된 RMB는 친환경적이고 2차 오염문제를 극복할 수 있는 물질로서, 비소의 자연저감 능력을 향상시킬 수 있는 정화처리제로 활용이 가능할 것으로 판단된다.

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Survey of Corrosion Cost in China and Preventive Strategies

  • Ke, Wei;Li, Zhiqiang
    • Corrosion Science and Technology
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    • v.7 no.5
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    • pp.259-264
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    • 2008
  • A national consultative project entitled "corrosion cost survey in China and preventive strategies" was funded by the Chinese Academy of Engineering in 1998. Soon afterwards, an expert group was organized jointly by the Institute of Metal Research, CAS and Chinese Society of Corrosion and Protection. The report on corrosion cost survey in China was published in 2003. According to this report the overall annual corrosion cost in China estimated by the Uhlig Method and Hoar Method at 1997-2001 was found to be 200.7 billion Yuan RMB and 228.8 billion Yuan RMB respectively, which is equivalent to 2% of the gross national product of China. However the total cost of corrosion including the direct and indirect cost was estimated to be more than 500 billion Yuan RMB per year in China. Among them, corrosion cost of infrastructure ranked in first comparing with other sectors. Although corrosion costs in some sectors, such as electric power, petrochemical, oil pipeline and railway in China has reduced in the past years, significant losses are still being encountered in most sectors of industries and cost-effective methods have not always been implemented. Both successful and unsuccessful cases in corrosion control and corrosion management were collected. As the investment in capital construction continues increasing rapidly in China, the maintenance and life extension of the infrastructures will become a big issue. The preventive strategies have been suggested

The Impact of China Exchange Rate Policy on its Trading Partners: Evidence Based on the GVAR Model

  • ABBAS, Shah;NGUYEN, Van Chien;YANFU, Zhu;NGUYEN, Huu Tinh
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.8
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    • pp.131-141
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    • 2020
  • This study is designed to investigate the impact of China exchange rate policy on its trading partners by using a country multi-dataset GVAR model. Our model includes samples of 30 countries, six from high-income, six from middle-income and eighteen from low-income countries. This study used annual time series data over the period 1992 to 2017. We constructed currency misalignment index and it provided some interesting features about the currency undervaluation and overvaluation. The results of the currency misalignment shows that China's Renminbi is structurally more undervalued over the sample period as compared to other countries, and fluctuation in major currencies effects the global trade around the world. The overall empirical results of the GVAR model indicate that RMB undervaluation affects the trade pattern and macroeconomic performance of China's trading partners. Overall, China's exchange rate undervaluation has mixed effects on trading partner's GDP, exports and imports. The devaluation of China's RMB efficiently stimulated China's exports and reduced imports. While, in some countries, this effect is reverse, the RMB undervaluation increases the GDP of partner countries and also increases their exports to China. The results confirm the strong and leading role of the Chinese Renminbi in the global trade.