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Effect of Morphological Factors, Antibiotics and Agrobacterium Co-cultivation in the Efficiency of Somatic Embryogenesis of Eggplant (Solanum melongena L.)

  • Claudia Magioli;Erika Tarre;Krul, William-Robert;Elisabeth Mansur
    • Journal of Plant Biotechnology
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    • v.3 no.1
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    • pp.19-25
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    • 2001
  • Induction of somatic embryogenesis from Brazilian eggplant variety F-100 was studied in response to four auxin types. NAA, at the optimal concentration of 54 $\mu\textrm{m}$, was the only one that resulted in the induction of somatic embryos in either leaf and cotyledon explant and, at murk lower intensity and frequency, in hypocotyl and epicotyl explants. The optimal temperatures for embryo induction were 28 and 35$^{\circ}C$ for cotyledon and leaf explants. Incubation at 22$^{\circ}C$ caused a significant reduction both in the frequency and intensity of induction. This system was used to study the effects of position and orientation of the tissue on the culture medium as well as of antibiotics and explant co-cultivation with Agrobacterium on the efficiency of somatic embryo induction. The intensity of embryo induction was greater in the midsections of cotyledons relative to apical and basal regions, when the abaxial surface was in contact with the culture medium. The presence of antibiotics resulted in approximately 40-60% reduction of embryo induction relative to control explants, which originated 335$\pm$26.6 embryos. Co-cultivation with Agrobacterium before treatment with antibiotics caused a more drastic reduction (80-99%). Ampicilin treatment after cocultivalion with Agrobacterium caused the least inhibitory effect, allowing the production of 60 embryos/explant.

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A Review on The EIA System of Each Country and Its Implication (각국의 환경영향평가제도와 시사점)

  • Oh Tae-Kon
    • The Journal of the Korea Contents Association
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    • v.5 no.4
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    • pp.62-70
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    • 2005
  • Since the announcement of the Rio Earth Charter in 1992, the Environmental Policy of each country has changed a lot. That is, it aims at compatibility of nature and humans, and preservation and development of environment. It has changed a goal from the past direct regulation and post-treatment to 'sustainable development'. To overcome world-wide environmental problems such as changing global climate, various political means such as economic incentives and pre-prevention policy such as environmentally-friendly productive process, effective use of resources, minimized generation of waste have been developed. As environmental problems which are growing serious began to threat survival of human beings, they must be globally managed rather than by a single country. Therefore, this study is to speculate the EIA System of each country for environmental protection based on such recognition of problems.

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Random Regression Models Are Suitable to Substitute the Traditional 305-Day Lactation Model in Genetic Evaluations of Holstein Cattle in Brazil

  • Padilha, Alessandro Haiduck;Cobuci, Jaime Araujo;Costa, Claudio Napolis;Neto, Jose Braccini
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.6
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    • pp.759-767
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    • 2016
  • The aim of this study was to compare two random regression models (RRM) fitted by fourth ($RRM_4$) and fifth-order Legendre polynomials ($RRM_5$) with a lactation model (LM) for evaluating Holstein cattle in Brazil. Two datasets with the same animals were prepared for this study. To apply test-day RRM and LMs, 262,426 test day records and 30,228 lactation records covering 305 days were prepared, respectively. The lowest values of Akaike's information criterion, Bayesian information criterion, and estimates of the maximum of the likelihood function (-2LogL) were for $RRM_4$. Heritability for 305-day milk yield (305MY) was 0.23 ($RRM_4$), 0.24 ($RRM_5$), and 0.21 (LM). Heritability, additive genetic and permanent environmental variances of test days on days in milk was from 0.16 to 0.27, from 3.76 to 6.88 and from 11.12 to 20.21, respectively. Additive genetic correlations between test days ranged from 0.20 to 0.99. Permanent environmental correlations between test days were between 0.07 and 0.99. Standard deviations of average estimated breeding values (EBVs) for 305MY from $RRM_4$ and $RRM_5$ were from 11% to 30% higher for bulls and around 28% higher for cows than that in LM. Rank correlations between RRM EBVs and LM EBVs were between 0.86 to 0.96 for bulls and 0.80 to 0.87 for cows. Average percentage of gain in reliability of EBVs for 305-day yield increased from 4% to 17% for bulls and from 23% to 24% for cows when reliability of EBVs from RRM models was compared to those from LM model. Random regression model fitted by fourth order Legendre polynomials is recommended for genetic evaluations of Brazilian Holstein cattle because of the higher reliability in the estimation of breeding values.

Health-Related Quality of Life, Depression and Anxiety in Hospitalized Patients with Tuberculosis

  • Santos, Ana Paula Cere dos;Lazzari, Tassia Kirchmann;Silva, Denise Rossato
    • Tuberculosis and Respiratory Diseases
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    • v.80 no.1
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    • pp.69-76
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    • 2017
  • Background: Much of the attention of tuberculosis (TB) programs is focused on outcomes of microbiological cure and mortality, and health related quality of life (HRQL) is undervalued. Also, TB patients have a significantly higher risk of developing depression and anxiety compared with those in the general population. We intend to evaluate the HRQL and the prevalence of symptoms of depression and anxiety in hospitalized patients with TB. Methods: Cross-sectional study in a tertiary care hospital in Brazil. Adult patients with pulmonary TB that were hospitalized during the study period were identified and invited to participate. HRQL was measured using the Medical Outcomes Study Short Form-36 (SF-36) version 2. Hospital Anxiety and Depression Scale (HADS) was used to record symptoms of anxiety and depression. Results: Eighty-six patients were included in the analysis. The mean age of all patients was $44.6{\pm}15.4$ years, 69.8% were male, and 53.5% were white. Thirty-two patients (37.2%) were human immunodeficiency virus positive. Twenty-seven patients (31.4%) met study criteria for depression (HADS depression score ${\geq}11$) and 33 (38.4%) had anxiety (HADS anxiety score ${\geq}11$). Scores on all domains of SF-36 were significantly lower than the Brazilian norm scores (p<0.001). Conclusion: The present study shows that TB patients may have a poor HRQL. Additionally, we found a possible high prevalence of depression and anxiety in this population. Health care workers should be aware of these psychological disorders to enable a better management of these patients. The treatment of these comorbidities may be associated with better TB outcomes.

Structural analysis of a prestressed segmented girder using contact elements in ANSYS

  • Lazzari, Paula M.;Filho, Americo Campos;Lazzari, Bruna M.;Pacheco, Alexandre R.
    • Computers and Concrete
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    • v.20 no.3
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    • pp.319-327
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    • 2017
  • Studying the structural behavior of prestressed segmented girders is quite important due to the large use this type of solution in viaducts and bridges. Thus, this work presents a nonlinear three-dimensional structural analysis of an externally prestressed segmented concrete girder through the Finite Element Method (FEM), using a customized ANSYS platform, version 14.5. Aiming the minimization of the computational effort by using the lowest number of finite elements, a new viscoelastoplastic material model has been implemented for the structural concrete with the UPF customization tool of ANSYS, adding new subroutines, written in FORTRAN programming language, to the main program. This model takes into consideration the cracking of concrete in its formulation, being based on fib Model Code 2010, which uses Ottosen rupture surface as the rupture criterion. By implementing this new material model, it was possible to use the three-dimensional 20-node quadratic element SOLID186 to model the concrete. Upon validation of the model, an externally prestressed segmented box concrete girder that was originally lab tested by Aparicio et al. (2002) has been computationally simulated. In the discretization of the structure, in addition to element SOLID186 for the concrete, unidimensional element LINK180 has been used to model the prestressing tendons, as well as contact elements CONTA174 and TARGE170 to simulate the dry joints along the segmented girder. Stresses in the concrete and in the prestressing tendons are assessed, as well as joint openings and load versus deflection diagrams. A comparison between numerical and experimental data is also presented, showing a good agreement.

Modification of polyamide reverse osmosis membranes seeking for better resistance to oxidizing agents

  • Silva, Lucinda F.;Michel, Ricardo C.;Borges, Cristiano P.
    • Membrane and Water Treatment
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    • v.3 no.3
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    • pp.169-179
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    • 2012
  • One of the major limitations in the use of commercial aromatic polyamide thin film composite (TFC) reverse osmosis (RO) membranes is to maintain high performance over a long period of operation, due to the sensitivity of polyamide (PA) skin layer to oxidizing agents, such as chlorine, even at very low concentrations in feed water. This article reports surface modification of a commercial TFC RO membrane (BW30-Dow Filmtec) by covering it with a thin film of poly(vinyl alcohol) (PVA) crosslinked with glutaraldehyde (GA) to improve its resistance to chlorine. Crosslinking reaction was carried out at 25 and $40^{\circ}C$ by using PVA 1.0 wt.% solutions at different GA/PVA mass ratio, namely 0.0022, 0.0043 and 0.013. Water swelling measurements indicated a maximum crosslinking density for PVA films prepared at $40^{\circ}C$ and GA/PVA 0.0043. ATR-FTIR and TGA analysis confirmed the reaction between GA and PVA. SEM images of the original and modified membranes were used to evaluate the surface coating. Chlorine resistance of original and modified membranes was evaluated by exposing it to an oxidant solution (NaClO 300 mg/L, NaCl 2,000 mg/L, pH 9.5) and measuring water permeability and salt rejection during more than 100 h period. The surface modification effectively was demonstrated by increasing the chlorine resistance of PA commercial membrane from 1,000 ppm.h to more than 15.000 ppm.h.

Review on Monitoring Volume Transport through Measurement of Submarine Cable Voltages around Korea (한반도 주변의 해저 케이블 전압측정을 통한 해수수송량 관측)

  • Kim, Yun-Bae;Kim, Young-Ho;Lyu, Sang-Jin;Kim, Kuh;Choi, Byeong-Ho
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.9 no.2
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    • pp.73-81
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    • 2004
  • Voltage induced by the Tsushima Current on an abandoned submarine telephone cable has been measured since March 1998 in order to monitor the volume transport through the Korea Strait. Voltage has a good linear relationship with the transport measured by bottom-mounted Acoustic Doppler Current Profilers (ADCPs) along a section spanning the Korea Strait. This paper reviews researches using submarine cables briefly; we introduce the method to measure voltage on submarine cables, present some results focusing on the example of the Korea Strait, and mention the future prospect to use other submarine cables around Korea.

International Rule for Environment and International Trade (국제환경규범(國際環境規範)과 무역연계(貿易連繫))

  • Shin, Han-Dong
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.12
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    • pp.587-613
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    • 1999
  • Environmental problems such as global climate change, depletion, ocean and air pollution, and resource degradation-compounded by an expanding world population-respect no border and threaten the health, prosperity and jobs of all mankind. Our efforts to promote democracy, free trade, and stability in the world will fall short unless people have a livable environment. We have an enormous stake in the management of the world's resources. By increasing demand for timber, natural gas, coal and consumer's goods have destroyed the grounds for living. Greenhouse gas emissions anywhere in the world have threatened coastal communities, and then changed the Earth's climate system. The burning of coal, oil, and other fossil fuels is increasing substantially the concentration of heat-trapping gasses such as carbon dioxide, methane, and nitrous oxide in our air. The earth's temperature and sea levels are rising as a result. Since 1972 there has been a marked growth in the number and scope of environmental treaties. In particular, after the 1992 Rio Conference, international legal instruments became more concentrated on addressing environment within the context of sustainable development and incorporated a number of new concepts and innovative approaches. A preliminary analysis of recent conventions and in particular those associated with the Rio Conference indicates various ideas, concepts and principles which have come to the fore including sustainable development, equity, common concern of humankind, common but differentiated responsibilities and global partnership. However, international trade also has an environmental impact which must be minimized or countered. Positive measures are to be preferred to achieve environmental goals, but where trade provisions are necessary, they should be appropriately used within environmental conventions to facilitate the reduction and limitation of the negative impacts of trade and to enhance the complementarity of the multilateral trade regime with the imperatives of environmental protection, in the interests of environmental protection and sustainable development generally. The international community has to recognize and endorse this need to achieve complementarity between trade and environment issues.

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Genetic Parameters for Linear Type Traits and Milk, Fat, and Protein Production in Holstein Cows in Brazil

  • Campos, Rafael Viegas;Cobuci, Jaime Araujo;Kern, Elisandra Lurdes;Costa, Claudio Napolis;McManus, Concepta Margaret
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.4
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    • pp.476-484
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    • 2015
  • The objective of this study was to estimate genetic and phenotypic parameters for linear type traits, as well as milk yield (MY), fat yield (FY) and protein yield (PY) in 18,831 Holstein cows reared in 495 herds in Brazil. Restricted maximum likelihood with a bivariate model was used for estimation genetic parameters, including fixed effects of herd-year of classification, period of classification, classifier and stage of lactation for linear type traits and herd-year of calving, season of calving and lactation order effects for production traits. The age of cow at calving was fitted as a covariate (with linear and quadratic terms), common to both models. Heritability estimates varied from 0.09 to 0.38 for linear type traits and from 0.17 to 0.24 for production traits, indicating sufficient genetic variability to achieve genetic gain through selection. In general, estimates of genetic correlations between type and production traits were low, except for udder texture and angularity that showed positive genetic correlations (>0.29) with MY, FY, and PY. Udder depth had the highest negative genetic correlation (-0.30) with production traits. Selection for final score, commonly used by farmers as a practical selection tool to improve type traits, does not lead to significant improvements in production traits, thus the use of selection indices that consider both sets of traits (production and type) seems to be the most adequate to carry out genetic selection of animals in the Brazilian herd.