• Journal of the Korean Society for Nondestructive Testing
• /
• v.23 no.3
• /
• pp.220-226
• /
• 2003

This paper presents the result of an investigation on the application of the acoustic emission method to the monitoring of fatigue crack initiation, growth and track location in welded joints. Fatigue test was carried out for a typical fillet welded joint of ship structure. AE parameter such as ring down count was analyzed in time domain and crack locations were examined by source location and cluster option which is one of the functions of AE signal processor The usability of AE mettled was confirmed for the detection of the initiation and location of through crack.

• Archives of Pharmacal Research
• /
• v.20 no.5
• /
• pp.454-458
• /
• 1997

Two human liver UDP-glucuronosyltransferase cDNA clones, HLUG25 and UDPGTh2 were previously shown to encode isozymes active in the glucuronidation of hyodeoxycholic acid (HDCA) and certain estrogen derivatives (e.g., estriol and 3,4-catechol estrogens), respectively. in this study we have found that the UDPGTh2-encoded isoform (UDPGTh2) and HLUG25-encoded isoform (UDPGThl) have parallel aglycone specificities. When expressed in COS 1 cells, each isoform metabolized three types of dihydroxy- or trihydroxy-substituted ring structures, including the 3,4-catechol estrogen (4-hydroxyestrone), estriol, 17-epiestriol, and HDCA, but the UDPGTh2 isozyme was 100-fold more efficient than UDPGTh1. UDPGTh1 and UDPGTh2 were 86% identical overall (76 differences out of 528 amino acids), including 55 differences in the first 300 amino acids of the amino terminus, a domain which conferred the substrate specificity. The data indicated that a high level of conservation in the amino terminus was not required for the preservation of substrate selectivity. Analysis of glucuronidation activity encoded by UDPGTh1/UDPGTh2 chimeric cDNA constructed at their common restriction sites, Sac I (codon 297), Nco I (codon 385), and Hha I (codon 469), showed that nine amino acids between residues 385 and 469 were important for catalytic efficiency, suggesting that this region represented a domain which was critical for the catalysis but distinct from that responsible for aglycone-selection. These data indicate that UDPGTh2 is a primary isoform responsible for the detoxification of the bile salt intermediate as well as the active estrogen intermediates.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.12 no.3
• /
• pp.99-114
• /
• 2012

Proxy Mobile IPv6 (PMIPv6) has received considerable attention between telecommunications and the Internet communities and does not require active participation of the Mobile Node (MN) by way of network-based mobility management. The PMIPv6 domain is studying establishment in progress to support extensively a number of MN by using a low handover latency. In this research, we are propose a novel 3S scheme for building Scalable and Secure and Seamless PMIPv6 domains. In the proposed scheme, all of Mobility Access Gateway (MAG) are acting as the Local Mobility Anchor (LMA) and composing a virtual ring with another MAG. General hashing is used in the efficient distribution-mapping between each MN and the MN's LMA of all MAGs. And, MAG and MN are authenticated using the symmetric key. Through mathematical analysis, we verifies the safety, scalability, and seamless service for 3S. Also, we're propose a handover procedure of 3S and show better than the existing schemes in terms of handover latency.

• Mycobiology
• /
• v.42 no.1
• /
• pp.34-40
• /
• 2014

Usnea longissima has a long history of use as a traditional medicine. Several bioactive compounds, primarily belonging to the polyketide family, have been isolated from U. longissima. However, the genes for the biosynthesis of these compounds are yet to be identified. In the present study, three different types of non-reducing polyketide synthases (UlPKS2, UlPKS4, and UlPKS6) were identified from a cultured lichen-forming fungus of U. longissima. Phylogenetic analysis of product template domains showed that UlPKS2 and UlPKS4 belong to group IV, which includes the non-reducing polyketide synthases with an methyltransferase (MeT) domain that are involved in methylorcinol-based compound synthesis; UlPKS6 was found to belong to group I, which includes the non-reducing polyketide synthases that synthesize single aromatic ring polyketides, such as orsellinic acid. Reverse transcriptase-PCR analysis demonstrated that UlPKS2 and UlPKS4 were upregulated by sucrose; UlPKS6 was downregulated by asparagine, glycine, and alanine.

• BMB Reports
• /
• v.53 no.2
• /
• pp.112-117
• /
• 2020

A recent study suggested that methylation of ubiquitin-like with PHD and RING finger domain 1 (UHRF1) is regulated by SET7 and lysine-specific histone demethylase 1A (LSD1) and is essential for homologous recombination (HR). The study demonstrated that SET7-mediated methylation of UHRF1 promotes polyubiquitination of proliferating cell nuclear antigen (PCNA), inducing HR. However, studies on mediators that interact with and recruit UHRF1 to damaged lesions are needed to elucidate the mechanism of UHRF1 methylation-induced HR. Here, we identified that poly [ADP-ribose] polymerase 1 (PARP1) interacts with damage-induced methylated UHRF1 specifically and mediates UHRF1 to induce HR progression. Furthermore, cooperation of UHRF1-PARP1 is essential for cell viability, suggesting the importance of the interaction of UHRF1-PARP1 for damage tolerance in response to damage. Our data revealed that PARP1 mediates the HR mechanism, which is regulated by UHRF1 methylation. The data also indicated the significant role of PARP1 as a mediator of UHRF1 methylation-correlated HR pathway.

• Journal of the Korea Computer Industry Society
• /
• v.5 no.8
• /
• pp.811-818
• /
• 2004

The spreading of internet, combined with the computerization of industry and the life whole has created an incereased demand on the private life protection and information portection, but due to the lack of specialty manpower on the information protection industry field there are many difficulties. Therefore, in this paper a curriculum for an information protection specialty manpower training is proposed. The proposed curriculum indispensability 1,2 and classified with a selection 1,2 and classified with a selection 1,2. The information protection application field into 9 segments of domains, and to carry out the curriculum in a ring structure. The curriculum based on the information security field's 9 domains and related field practical business, and the course offered after graduation to deepen the specialty, need to be carried out by each domain in order to continuously carry out the information security deepening process.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.6 no.5
• /
• pp.211-221
• /
• 1998

Thermal behavior on the cylinder block of a 4-cylinder, 4-stroke 2.0L SOHC gasoline engine was numerically and experimentally analyzed. The numerical calculation was performed using the finite element method. The cylinder block was modelled as a three dimensional finite element by considering its geometry. The physical domain was devided into hexahedron elements. 16 thermocouples were installed at points of 2mm inside from cylinder wall near top ring of piston in cylinder block, which points have suffered major thermal loads and suggested as proper measurement points for engine design by industrial engineers. Under full load and 9$0^{\circ}C$ coolant temperature condition, temperature behavior of cylinder block according to engine speed were analyzed. The results showed that temperature rose gradually to conform to a function of 2nd~4th order of engine speed at intake side, exhaust and siamese side, respectively. As engine load was changed from 100 to 50% by 25% step, temperature curve also conformed to 2nd~7th order function of engine speed. Temperature differences by load condition were similar among 100, 75% and 50%. Under full load and coolant temperature of 11$0^{\circ}C$, temperature behavior were also analyzed and the result also showed conformance to 2n d~7th order function of engine speed. Temperature curve was transferred in parallel upwards corresponding coolant temperature rise.

• 한국신재생에너지학회:학술대회논문집
• /
• 2011.05a
• /
• pp.62.1-62.1
• /
• 2011

The wind can be stronger and steadier further from shore, but water depth is also deeper. Then bottom-mounted towers are not feasible, and floating turbines are more competitive. There are additional motions in an offshore floating wind turbine, which results in a more complex aerodynamics operating environment for the turbine rotor. Many aerodynamic analysis methods rely on blade element momentum theory to investigate aerodynamic load, which are not valid in vortex ring state that occurs in floating wind turbine operations. So, vortex lattice method, which is more physical, was used in this analysis. Floating platform's prescribed positions were calculated in the time domain by using floating system RAO and waves that are simulated using JONSWAP spectrum. The average value of in-plane aerodynamic force increase, but the value of out-of-plane force decrease. The maximum variation aerodynamic force abruptly increases in severe sea state. Especially, as the pitch motion of the barge platform is large, this motion should be avoided to decrease the aerodynamic load variation.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.5
• /
• pp.764-770
• /
• 2006

Dihydrochalcomycin (GERI-155), produced by Streptomyces sp. KCTC-0041BP isolated from Korean soil, is a 16-membered macrolide antibiotic consisting of two deoxysugar moieties at C-5 and C-20 positions of a branched lactone ring. The cloning and sequencing of a gene cluster for dihydrochalcomycin biosynthesis revealed a 63-kb nucleotide region containing 25 open reading frames (ORFs). The products of all of these 25 ORFs playa role in dihydrochalcomycin biosynthesis and self-resistance against the compounds synthesized. At the core of this cluster lies a 39.6-kb polyketide synthase (PKS) region encoding eight modules in five giant multifunctional protein-coding genes (gerSI-SV). The genes responsible for the biosynthesis of deoxysugar moieties, D-chalcose and D-mycinose, and their modification and attachment were found on either side of this PKS region. The involvement of this gene cluster in dihydrochalcomycin biosynthesis was confirmed by disruption of the dehydratase (DH) domain in module 3 of the PKS gene and by metabolite analysis.

• Korean Journal of Optics and Photonics
• /
• v.20 no.3
• /
• pp.189-194
• /
• 2009

We demonstrate a ring type wavelength swept laser incorporating a fiber Fabry-Perot tunable filter in a laser cavity using 1300 nm semiconductor optical amplifier as a gain medium. The output characteristics of the wavelength swept laser according to the applied scanning frequencies are analyzed in the temporal and spectral domain. The output of the wavelength swept laser decreases dramatically as the scanning frequency increases. And there is a significant peak power imbalance between the forward scan and the backward scan as the scanning frequency increases. Its use in practical applications might be limited.