• The korean journal of orthodontics
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• v.26 no.2 s.55
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• pp.205-218
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• 1996

A given facial type can be considered as a syndrome in which various features are aggregated, so a single parameter is not sufficient to accurately identify a given facial type. This study was designed to identify & characterize the skeletal types that blend under the headline-'Cl III,deepbite'. Cephalograms of thirty-four untreated mixed dentition patients, selected mainly on the basis of clinical impression of Cl III with reduced lower face heights were studied. The following conclusion can be drawn. 1. Cl III malocclusion with reduced lower face height could be classified into three types. 2. Subtype 1 was identified by the following features : strong ramus, more anteriorly positioned upper molars without alveolar hypoplasia, acutely reduced Mn. plane angle. 3. Subtype 2 was characterized by a short ramus, sharply reduced postrior alveolar height, and normal Mn. plane angle. In general, this type had hypoplasia tendency in the vertical dimension. 4. In subtype 3, the AUFH occupying more percentage than ALFH was a outstanding feature. Ramal height was in normal range, alveolar hypoplasia and slightly reduced Mn. plane angle was observed. 5. The features of the subtypes were reflected in certain indices, which can be regarded as discriminative index. LAFH: if reduced, regardless of subtypes, indicates reduced lower ant. face height consistently. FHR: when this ratio is increased, it indicates subtype 1. FHI: when this ratio is in normal range, it indicates subtype 2. FPI: if reduced greatly, it indicates subtype 3.

• Journal of Periodontal and Implant Science
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• v.26 no.2
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• pp.469-490
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• 1996

The initial events required for periodontal regeneration is the attachment, spreading, and proliferation of appropriated cells at the healing sites. These have been reported that minocycline stimulates the attachment of periodontal ligament cells, and also $TGF-{\beta}1$ enhances the proliferation of periodontal ligament cells. The purpose of the present study was to evaluate the effects of $TGF-{\beta}1$ on the cellular activity of minocycline treated human periodontal ligament cells. Periodontal ligament cells were obtained from the explants of healthy periodontal ligaments of extracted 3rd molars or premolar teeth extracted from the patients for orthodontic treatment. The cells were cultured in minimal essential medium(${\alpha}-MEM$) supplemented with 10.000units/ml penicillin, $10,000{\mu}g/ml$ streptomycin and 10% FBS(fetal bovine serum) at $37^{\circ}C$ in a humidified atmosphere of 5% carbon dioxide and the 5th to the 8th passages of the cells were used. To evaluate the effect of minocycline on cell attachment, the cells were seeded at a cell density of $1.5{\times}10^4$ cells/well in 24-well culture plates and treated with $20{\mu}g/ml$ and $100{\mu}g/ml$ of minocycline for 1.5 h. After trypsinization, the cells were counted with hemocytometer and were taken photographs for observation of cellular morphology. To evaluate the effect of $TGF-{\beta}1$ on minocycline-pretreated periodontal ligament cells, the cells were seeded at a cell density of $1{\times}10^4$ cells/ well in 24-well culture plates and treated with $20{\mu}g/ml$ and $100{\mu}g/ml$ of minocycline for 1.5 h. After incubation, 1 and 10ng/ml of $rh-TGF-{\beta}1$ were also added to the each well and incubated for 1 and 2 days, respectively. Then, MTT assay, DNA synthesis($^3H-thymidine\;assay$), and protein and collagen assay(3H-proline assay) were carried out. In the MIT assay, after 200ul MTT solutionlconeentration of 5mg/ml) were added to the each well of the 24-well plates and incubated for 3 hours, and 200 ul DMSO were added so as to dissolve insoluble blue formazan crystals which was formed in incubated period. Then it read plates on a ELISA reader. For mitogenic assay, 1 uCi/ml $^3H-thymidine$ was added to each well for the final 2 hours of the incubation periods. After labeling, the wells were washed 3 times with ice cold PBS and 4 times with 5% TCA to remove unincorporated label and precipitate the cellular DNA. DNA, with the incorporated $^3H-thymidine$, was solubilized with 500 ul of 0.1% NaOH/0.1% SDS. A 250 ul aliquot was removed from each well and placed in a scintillation vial with 4ml of scintillation cocktail. Using an liguid scintillation counter, counts per minute(CPM) were determined for each samples. 3 uCi/ml $^3H-proline$ was added to each well for the final 4 hours of the incubation periods and total protein and percent collagen synthesis were carried out. The results indicate that minocycline treated group with $100{\mu}g/ml$ concentration for 1.5 hours significantly increased than that of control in cell attachment, and cell process is also evident compared with that of control in cell morphology, and the cellular activity and DNA synthesis rate of cells treated minocycline and $TGF-{\beta}1$ significantly increased than that of control values, but were below to values of the $TGF-{\beta}1$ only treated group in MIT assay and $^3H-thymidine\;assay$, and the total protein synthesis of minocycline and $TGF-{\beta}1$ treated group also significantly increased than that of control values, but the percent collagen synthesis of tested group significantly decreased to compared with control. On the above the findings, the tested group of minocycline and $TGF-{\beta}1$ did not increase the effect on the cell activity than $TGF-{\beta}1$ only tested group and the tested group of minocycline inhibited cell activity. This results indicate that minocycline was effective on cell attachment in early stage, but it is harmful to cell activity, that inhibitory effect of minocycline was compensated with stimulatory effect of $TGF-{\beta}1$.

• Korean journal of applied entomology
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• v.25 no.1 s.66
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• pp.41-46
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• 1986

Exogenous factor and nutrients affecting for conidial germination of Colletetrichum dematium f. sp. capsicum causing red pepper anthracnose were studied by slide germination test. Optimum temperature of conidial germination was at $28^{\circ}C$, ranging 15 to $35^{\circ}C$. Optimum pH was at 5.5, ranging 4.5 to 8.0, and more than 90% of relative humidity (RH) was optimum. Poor conidial germination of the fungus was observed on sterile distilled water, but potato sucrose broth (PSB), red pepper fruit broth (RPFB), green pepper fruit broth (GPFB) and pepper leaf broth (PLB) furnished a satisfactory nutrients for conidial germination. Exogenous supply of carbon and nitrogen sources were essential for conidial germination, while potassium, phosphorous and sulfur were not evident as that for carbon and nitrogen. Soluble starch was the most suitable as a carbon source for conidial germination and followed by D-glucose, D-galactose and lactose in that order. Maximum germination was attained in the $1{\times}10^4$ conidia per ml. Germination was decreased with increment of conidial concentration, and in the density of $5{\times}10^4$ conida per ml, germination was nearly supipressed. It suggested existing a self-inhibitor. Non-washed conidia germinated more than washed conidia, and conidial germination was also gradually decreased by increasing conidial density.

• Korean journal of applied entomology
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• v.46 no.2
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• pp.213-219
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• 2007

The development of Schizaphis graminum (Rondani) was studied at various constant temperatures ranging from 15 to $32.5^{\circ}C$, with $65{\pm}5%$ RH, and a photoperiod of 16L:8D. Mortality of the $1_{st}-2_{nd}\;and\;the\;3_{rd}-4_{th}$ stage nymphs were similar at most temperature ranges while at high temperature of $32.5^{\circ}C$, more $3_{rd}-4_{th}$ stage individuals died. The total developmental time ranged from 13.8 days at $15^{\circ}C$ to 4.9 days at $30.0^{\circ}C$ suggesting that the higher the temperature, the faster the development. However, at higher end temperature of $32.5^{\circ}C$ the development took 6.4 days. The lower developmental threshold temperature and effective accumulative temperatures for the total immature stage were $6.8^{\circ}C$ and 105.9 day-degrees, respectively and the nonlinear shape of temperature related development was well described by the modified Sharpe and DeMichele model. The normalized cumulative frequency distributions of developmental period for each life stage were fitted to the three-parameter Weibull function. The attendance of shortened developmental times was apparent with $1_{st}-2_{nd}\;nymph,\;3_{rd}-4_{th}$ nymph, and total nymph stages in descending order. The coefficient of determination $r^2$ ranged between 0.80 and 0.87.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.305-312
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• 2010

The developmental time of immature stages of Paromius exiguus (Distant) was investigated at nine constant temperatures (15, 17.5, 20, 22.5, 25, 27.5, 30, 32.5, $35{\pm}1^{\circ}C$), 20-30% RH, and a photoperiod of 14:10h (L:D). Eggs did not develop at $15^{\circ}C$, and their developmental time decreased with increasing temperatures. Its developmental time was longest at $17.5^{\circ}C$ (28.2 days) and shortest at $35^{\circ}C$ (5.9 days). The first nymphs failed to reach the next nymphal stage at 17.5 and $35^{\circ}C$. Nymphal developmental time decreased with increasing temperatures between $20^{\circ}C$ and $32.5^{\circ}C$, and developmental rate was decreased at temperatures above $30^{\circ}C$ in all stages except for the fourth nymphal stage. The relationship between developmental rate and temperature fit a linear model and three nonlinear models (Briere 1, Lactin 2, and Logan 6). The lower threshold temperature of egg and total nymphal stage was $l3.8^{\circ}C$ and $15.3^{\circ}C$, respectively. The thermal constant required to reach complete egg and the total nymphal stage was 109.9 and 312.5DD, respectively. The Logan-6 model was best fitted ($r^2$=0.94-0.99), among three nonlinear models. The distribution of completion of each development stage was well described by the 3-parameter Weibull function ($r^2$=0.91-0.99).

• Korean journal of applied entomology
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• v.56 no.4
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• pp.319-329
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• 2017

We investigated the host preference of Ricania app. at different developmental stages to select the effective control methods. Ricania spp. at different developmental stages preferred different host plants. During the nymphal period, individuals moved to the bottom due to environmental factors such as wind and rain, rather than inhabiting trees, and fed on various plants ranging from 1 year-old herbaceous plants to shrubs. Especially, at Palbong Mt. in Gonju, Ricania spp. nymphs preferred Symplocos chinensis f. pilosa, Celastrus orbiculatus, Euonymus alatus, Kalopanax septemlobus, Aralia elata, Styrax japonicas, Rubus parvifolius, Ru. coreanus, Ru. crataegifolius, Quercus acutissima, Cornus officinalis, Lespedeza bicolor, Albizia julibrissin, L. maximowiczii, Rhododendron schlippenbachii and Rh. indicum. At Yongbong Mt. in Hongseong, Ricania spp. adults preferred herbaceous plants, such as Helianthus annuus, Solanum nigrum, and Oenothera biennis rather than trees during the pre-oviposition stage. Subsequently, as the oviposition season began, Ricania spp. adults were rarely found in H. annuus, S. nigrum, or O. biennis and appeared to migrate to the perennial trees and shrubs hosts to lay eggs. At Palbong Mt., the main laying hosts of Ricania spp. adults were Weigela subsessilis, Ailanthus altissima, Q. aliena, Boehmeria platanifolia, Diospyros lotus, Ziziphus jujubae var. inermis, St. japonicus, Prunus mume, P. serrulata var. spontanea, Spiraea prunifolia f. simpliciflora, Ru. crataegifolius, Salix koreensis, Ru. yedoense f. poukhanense, and Co. controversa. The degree of egg laying, number of egg masses and adult Ricania spp. were significantly higher in sunlit areas than in shaded areas, in addition, the lower the altitude, the more adults were present. This study contributes toward the development of traps that can attract and control Ricania spp. and reduce the density of Ricania spp. that disturb the ecosystem.

• Journal of Bio-Environment Control
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• v.27 no.4
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• pp.312-318
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• 2018

We investigated the effect of postharvest treatments of calcium chloride, lysophosphatidyl ethanolamine (LPE) or 1-methylcyclopropene (1-MCP) on fruit quality during simulated marketing in Asian pear (Pyrus pyrifolia Nakai). 'Whangkeumbae' pear fruits were immersed in 0.25, 0.5 or 1.0% $CaCl_2$ solution with or without ultrasound (40kHz) at $25^{\circ}C$ for 3min followed by storage at $1^{\circ}C$ for 30 days simulated as abroad exportation. After simulated marketing at $25^{\circ}C$ and 80% relative humidity (RH) up for 10 days, quality parameters were evaluated. Results indicated that the ultrasound and $CaCl_2$ treatment had a synergic effect on keeping the green skin color which showed lower $a^*$ value. The combination treatment of ultrasound and 0.5% and 1.0% $CaCl_2$ significantly reduced internal browning disorders, although severe skin blemish disorder (20-23%) occurred in 1.0% $CaCl_2$ treatment. 'Wonhwang' pears were immersed in 1,000ppm LPE for 3 minutes or were fumigated in 1,000 ppb 1-MCP for 12 hours, respectively. The results of the fruit quality survey during the 21 days of distribution period are as follows. The 1-MCP treatment was maintained at a constant flesh firmness of 33N or higher during the distribution period. The LPE treated fruits had a lower physiological disorder index than the untreated group, but showed a relatively higher value than the 1-MCP treated group. In the case of 1-MCP treatment, the fruit respiration rate was significantly lower than of untreated control ($6.0mL{\cdot}kg^{-1}{\cdot}hr^{-1}$) during the simulaed marketing period. Consequently, it was expected that the postharvest treatments of 0.5% calcium chloride in pararell with ultrasound and 1-MCP fumigation can help to maintain Asian pear quality during distribution period.

• Korean journal of applied entomology
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• v.50 no.4
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• pp.343-352
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• 2011

The developmental period of Laodelphax striatellus Fallen, a vector of rice stripe virus (RSV), was investigated at ten constant temperatures from 12.5 to $35{\pm}1^{\circ}C$ at 30 to 40% RH, and a photoperiod of 14:10 (L:D) h. Eggs developed successfully at each temperature tested and their developmental time decreased as temperature increased. Egg development was fasted at $35^{\circ}C$(5.8 days), and slowest at $12.5^{\circ}C$ (44.5 days). Nymphs could not develop to the adult stage at 32.5 or $35^{\circ}C$. The mean total developmental time of nymphal stages at 12.5, 15, 17.5, 20, 22.5, 25, 27.5 and $30^{\circ}C$ were 132.7, 55.9, 37.7, 26.9, 20.2, 15.8, 14.9 and 17.4 days, respectively. One linear model and four nonlinear models (Briere 1, Lactin 2, Logan 6 and Poikilotherm rate) were used to determine the response of developmental rate to temperature. The lower threshold temperatures of egg and total nymphal stage of L. striatellus were $10.2^{\circ}C$ and $10.7^{\circ}C$, respectively. The thermal constants (degree-days) for eggs and nymphs were 122.0 and 238.1DD, respectively. Among the four nonlinear models, the Poikilotherm rate model had the best fit for all developmental stages ($r^2$=0.98~0.99). The distribution of completion of each development stage was well described by the two-parameter Weibull function ($r^2$=0.84~0.94). The emergence rate of L. striatellus adults using DYMEX$^{(R)}$ was predicted under the assumption that the physiological age of over-wintered nymphs was 0.2 and that the Poikilotherm rate model was applied to describe temperature-dependent development. The result presented higher predictability than other conditions.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.431-438
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• 2012

The developmental time of the nymphs of Myzus persicae was studied in the laboratory (six constant temperatures from 15 to $30^{\circ}C$ with 50~60% RH, and a photoperiod of 14L:10D) and in a green-pepper plastic house. Mortality of M. persicae in laboratory was high in the first(6.7~13.3%) and second instar nymphs(6.7%) at low temperatures and high in the third (17.8%) and fourth instar nymphs(17.8%) at high temperatures. Mortality was 66.7% at $33^{\circ}C$ in laboratory and $26.7^{\circ}C$ in plastic house. The total developmental time was the longest at $14.6^{\circ}C$ (14.4 days) and shortest at $26.7^{\circ}C$ (6.0 days) in plastic house. The lower threshold temperature of the total nymphal stage was $3.0^{\circ}C$ in laboratory. The thermal constant required for nymphal stage was 111.1DD. The relationship between developmental rate and temperature was fitted nonlinear model by Logan-6 which has the lowest value on Akaike information criterion (AIC) and Bayesian information criterion (BIC). The distribution of completion of each developmental stage was well described by the 3-parameter Weibull function ($r^2=0.95{\sim}0.97$). This model accurately described the predicted and observed occurrences. Thus the model is considered to be good for use in predicting the optimal spray time for Myzus persicae.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.421-429
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• 2012

The developmental time period of Aphis gossypii was studied in laboratory (six constant temperatures from 15 to $30^{\circ}C$ with 50~60% RH, and a photoperiod of 14L:10D) and in a cucumber plastic house. The mortality of A. gossypii in the laboratory was high in the 2nd (20.0%) and 3rd stage(13.3%) at low temperature but high in the 3rd (26.7%) and 4th stage (33.3%) at high temperatures. Mortality in the plastic house was high in the 1st and 2nd stage but there was no mortality in the 4th stage at low temperature. The total developmental period was longest at $15^{\circ}C$ (12.2 days) in the laboratory and shortest at $28.5^{\circ}C$ (4.09 days) in the plastic house. The lower threshold temperature at the total nymphal stage was $6.8^{\circ}C$ in laboratory. The thermal constant required to reach the total nymphal stage was 111.1DD. The relationship between the developmental rate and temperature fit the nonlinear model of Logan-6 which has the lowest value for the Akaike information criterion(AIC) and Bayesian information criterion(BIC). The distribution of completion of each development stage was well described by the 3-parameter Weibull function ($r^2=0.89{\sim}0.96$). This model accurately described the predicted and observed outcomes. Thus it is considered that the model can be used for predicting the optimal spray time for Aphis gossypii.