• Molecules and Cells
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• v.44 no.10
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• pp.770-779
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• 2021

Transgenic Arabidopsis thaliana expressing an anti-rabies monoclonal antibody (mAb), SO57, was obtained using Agrobacterium-mediated floral dip transformation. The endoplasmic reticulum (ER) retention signal Lys-Asp-Glu-Leu (KDEL) was tagged to the C-terminus of the anti-rabies mAb heavy chain to localize the mAb to the ER and enhance its accumulation. When the inaccurately folded proteins accumulated in the ER exceed its storage capacity, it results in stress that can affect plant development and growth. We generated T₁ transformants and obtained homozygous T₃ seeds from transgenic Arabidopsis to investigate the effect of KDEL on plant growth. The germination rate did not significantly differ between plants expressing mAb SO57 without KDEL (SO plant) and mAb SO57 with KDEL (SOK plant). The primary roots of SOK agar media grown plants were slightly shorter than those of SO plants. Transcriptomic analysis showed that expression of all 11 ER stress-related genes were not significantly changed in SOK plants relative to SO plants. SOK plants showed approximately three-fold higher mAb expression levels than those of SO plants. Consequently, the purified mAb amount per unit of SOK plant biomass was approximately three times higher than that of SO plants. A neutralization assay revealed that both plants exhibited efficient rapid fluorescent focus inhibition test values against the rabies virus relative to commercially available human rabies immunoglobulins. KDEL did not upregulate ER stress-related genes; therefore, the enhanced production of the mAb did not affect plant growth. Thus, KDEL fusion is recommended for enhancing mAb production in plant systems.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.183-188
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• 2021

The increase in temperature due to climate warming is predicted to affect crop yields in the future. Until now, various types of OTC (open top chamber) that simulate the future climate condition have been developed and used to study the effect of temperature increase due to global warming on maize growth. However, in most OTCs, high equipment and maintenance costs were required to artificially increase the temperature. This study was carried to develop a cost-effective and simple OTC suitable for climate warming experiments for forage maize. Three octagonal OTCs with a height of 3.5 m × a diameter of 4.08 m and a partially covered top were constructed. The lower part of OTC covered film was opened at a height of 26 cm (OTC-26), 12 cm (OTC-12) from the ground surface, or not opened (0 cm, OTC-0). Mean air temperatures during the daytime on a sunny day in OTC-0, OTC-12 and OTC-26 increased to 3.23℃, 1.33℃, and 0.89℃, respectively, compared to the ambient control plot. For a pilot test, forage maize, 'Gwangpyeongok' was grown at OTCs and ambient control plots. As a result, in the late maize vegetative growth phase (July 30), the plant height was increased more than 45% higher than the ambient control plot in all OTC plots, and the stem diameter also increased in all OTC plots. These results indicate that it is possible to set the temperature inside the OTC by adjusting the opening height of the lower end of the OTC, and it can be applied to study the response of forage maize to elevated temperature. An OTC, with its advantages of energy free, low maintenance cost, and simple temperature setting, will be helpful in studying maize growth responsiveness to climate warming in the future.

• Korean Journal of Breeding Science
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• v.42 no.2
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• pp.157-162
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• 2010

This study was carried out to develop of macro-protocol and the biosafety guide for 'Nakdong', and 'Golden Rice' (genetically modified vitamin A rice) by large scale field trial of GM crops. Typically, when a new GM crop is created, breeders should conduct field test to make sure the GM crop is safe, and provide some information on GM crops for approval. A total of isolated 4,700 $m^2$ field for trial of GM crops were prepared, and 'Nakdong' and 'Golden rice' were cultivated by standard method of RDA (Rural Development Administration, Korea). Field studies indicated that the population densities of insect pests and natural enemies have no difference between two varieties. While insect pest density on 'Nakdong' was slightly higher than on Golden Rice, but natural enemy density on Golden Rice was a little higher. These results provided the insect diversity for risk assessment analysis of Golden Rice and suggested that the macro-protocol could be useful to detect GM plants.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.46-46
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• 2019

The experiments were performed in the Jinan (elevation: 300 meters above sea level), Jeollabuk-do. Seedlings (n = 63 per $3.3m^2$) of ginseng cultivar (Cheonpung, Yeonpung) were planted on April 10, 2015. Shading material of plastic film house was blue-white film. Before the Planting seedling, silicate (3 kg/10 a) or chitosan (40 kg/10 a) was fertilized and foliar sprayed on the leaves 1000 times dilution solution once a month from May to September every year. The growth results of 5-year old ginseng surveyed in 2018 are as follows. The average air temperature in the plastic film house was the highest at $26.6^{\circ}C$ and $26.5^{\circ}C$ in July and August, respectively, and the highest temperature was $40.5^{\circ}C$ in July. The maximum daily temperature of $35^{\circ}C$ or more was 30 days, with the average soil temperature being $24.9^{\circ}C$ in August. The chemical properties of the test soil are as follows. pH was 6.4~6.9 level and EC was 0.35~0.46 dS/m. The organic matter content was 33.5~41.4 g/kg, and available-P content was 251.9~306.8 mg/kg. Exchangeable cations contents, such as K, Ca and Mg were all the appropriate ranges. The soil microbial density surveyed by the dilution plate method was 10~50 times higher than that of control (Non-treatment) and actinomycete density was 3~6 times higher. Pathogens of the genus Fusarium by Metagenome analysis decreased 91.3% and 68.2% respectively in the foliar sprayed of chitosan and soluble-silicate. The light intensity (PAR) in the blue-white film plastic film house gradually increased until July and then decereased, with the average of light intensity in March-October was $120.3umol/m^2/s$. The growth of aerial parts such as plant height and stem length was better than non-sprayed group in silicate or chitosan treatments and Yeonpung cultivar was superior to the Cheonpung cultivar. The SPAD value was higher in Yeonpung cultivar foliar sprayed with soluble-silicate. The growth of underground parts such as root length and taproot length were better in chitosan and soluble-silicate treatment than control, especially in Yeonpung cultivar foliar sprayed with chitosan was good in taproot length and taproot diameter, and fresh weight of root was 60.1 g. Ginsenoside contents were 24.9 mg/g and 22.4 mg/g, in the Cheonpung cultivar foliar sprayed with soluble-silicate or chitosan respectively, 28% and 15% higher than control (19.5 mg/g). The incidence of disease by Alteraria panax and Botrytis cinerea was 3~9% and 4~9%, respectively. High temperature damage rate was 3~5%.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.114-114
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• 2022

There are 28 families and 166 species of exotic weeds on agricultural land and among these, 23 families and 80 species of exotic weeds occur on pastures. Among them, the Solidago altissima is a perennial weed belonging to the asteraceae family and it is an exotic weed that spreads to the surrounding area using methods such as high seed production, vegetative propagation using underground rhizomes and allelochemical. Accordingly, in 2009, the Ministry of Environment designated it as an ecosystem-disrupting species. This study was conducted to obtain basic data about the effects of S.altissima derived allelochemicals on forage crops. The root of S.altissima was separated, dried in the shade and then pulverized to prepare an root powder. Powder was repeatedly extracted with methanol for 3 days and concentrated under reduced pressure to obtain an root methanol extract. Dissolve the extract in distilled water, dispense it in a separate-funnel and proceed with liquid-liquid extraction by adding equal amounts of n-haxane (Hex), chloroform (CHCI₃), ethyl acetate (EtoAC), and butanol (BuOH) in order of increasing polarity. A seed-bioassay was performed using fractions for each solvent, followed by separation and purification by silica gel column chromatography. As a result of the fraction germination test for each solvent, the IC₅₀ values using the fresh weight of each fraction were 898.3 mg L^-1, 676.3 mg L^-1, 1160 mg L^-1 and 1360 mg L^-1. CA, CB, and CC fractions were obtained through primary silica gel column chromatography that used CHCI₃ fraction. As a result of seed-bioassay using each fraction, the IC₅₀ values for the fresh weight of each fraction was 537.3 mg L^-1, 1280 mg L^-1 and 1947 mg L^-1. Based on this, 5 fractions were obtained as a result of secondary silica gel column chromatography using the CA fraction. A seed-bioassay was performed, as a result, the lowest IC₅₀ value was calculated as 226.7 mg L^-1 in the CAE fraction. Based on this, the fraction was analyzed by GC-MS. The results of this study can be used as basic research data on the effects of weeds on forage crops and allelochemicals secreted from S. altissima.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.17-22
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• 1999

Experiment was carried out to determine the agronomic characteristics, forage yield of silage corn(Zea mays L.) soil chemical characterictics and $NO_3-N$ level in infiltration water by different application rates of daily liquid manures. Four treatment consisting of chemical fetilizer, $200kg\;N\;ha^{-1}$, daily liquid manure $200kg\;N\;ha^{-1}$, $300kg\;N\;ha^{-1}$ and $400kg\;N\;ha^{-1}$ were arranged in a randonmized complete block design with three replicates. The results obtained at National Livestock Research Institute, RDA., in Suwon from 1996 to 1997 are summarized as follows; In plant height and crude protein content, it were appeared to highest by 264cm, 6.8% at the cattle slurry 150% plot(T3), respectively. but Dry matter yield(14.5MT/ha) and TDN(9.5MT/ha) production of chemical fertilizer plot(T1) were highest. Among daily liquid manure amount, dry matter yield(14.0MT/ha) and TDN yield(9.1MT/ha) of daily liquid manure 100% plot(T2) were resulted to 96% and 97% of chemical fertilizer plot(T1). End year's soil organic matter and phosphate content were appeared to high than those of beginning year in daily liquid manure plots, but it was not in proportioned to increase according to slurry amount. $NO_3-N$ level in infiltration water lower than 60cm of all plots were lowed than a permitted limit of cattle drinkable. As a result, it was appeared that the optimum application amount of dairy liquid manure was 200kg/ha by manure-N.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.169-176
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• 1997

Gibberellin (GA) has been widely used to break seed dormancy for better stand establishment. The experiment was done to clarify the effect of $GA_3$ (concentration; period) and light quality (red; white; dark) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum; Codonopsis lanceolata; C. pilosula) to get an information on their field emergence. The germination test was carried out with 12 hours irradiation for 9 days after priming treatment. In the darkness, the mean germination rate of all the species was decreased in the order to P. grandiflorum, C. lanceolata, C. pilosula. Their mean germination rates and radicle lengths were increased with increased concentration to 0.1mM of $GA_3$. Earlier germination rate was higher but later one was less in 4-day $GA_3$ treatment than in 1- or 2-day $GA_3$ treatment. Light treatment. especially red light given after $GA_3$ treatment. eliminated the $GA_3$ treatment effect. Red light done after $GA_3$ treatment nearly blocked the germination of P. grandiflorum and C. pilosula but delayed that of C. pilosula compared to the other light quality treatments having the similar rate. In addition. the radicle elongation of all three species affected by light quality treatment showed the same result as the germination rate.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1114-1119
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• 2012

This study was conducted to assess the microbial toxicity of ionic silver solution ($Ag^+N$) and silver nanoparticle suspension ($Ag^0NP$) based on the Microtox bioassay. In this test, the light inhibition of luminescent bacteria was measured after 15 and 30 min exposure to aqueous solutions and soils spiked with a dilution series of $Ag^+N$ and $Ag^0NP$. The resulting dose-response curves were used to derive effective concentration (EC25, $EC_{50}$, EC75) and effective dose ($ED_{25}$, $ED_{50}$, $ED_{75}$) that caused a 25, 50 and 75% inhibition of luminescence. In aqueous solutions, $EC_{50}$ value of $Ag^+N$ after 15 min exposure was determined to be < $2mg\;L^{-1}$ and remarkably lower than $EC_{50}$ value of $Ag^0NP$ with $251mg\;L^{-1}$. This revealed that $Ag^+N$ was more toxic to luminescent bacteria than $Ag^0NP$. In soil extracts, however, $ED_{50}$ value of $Ag^+N$ with 196 mg kg-1 was higher than $ED_{50}$ value of $Ag^0NP$ with $104mg\;kg^{-1}$, indicating less toxicity of $Ag^+N$ in soils. The reduced toxicity of $Ag^+N$ in soils can be attributed to a partial adsorption of ionic $Ag^+$ on soil colloids and humic acid as well as a partial formation of insoluble AgCl with NaCl of Microtox diluent. This resulted in lower concentration of active Ag in soil extracts obtained after 1 hour shaking with $Ag^+N$ than that spiked with $Ag^0NP$. With longer exposure time, EC and ED values of both $Ag^+N$ and $Ag^0NP$ decreased, so their toxicity increased. The toxic characteristics of silver nanomaterials were different depending on existing form of Ag ($Ag^+$, $Ag^0$), reaction medium (aqueous solution, soil), and exposure time.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.31-37
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• 1988

Germination and seedling elongation of soybeans [Glycine max. (L.) Merr., cults. Bangsakong, Hill, Paldalkong, Danyupkong, Baegwun-kong. Kwangkyokong, Changyupkong and Hwangkeumkong] were measured at two temperatures (15 and 30$^{\circ}C$) and four polyethylene glycol 10,000 (PEG) solutions (0, 20, 30 and 35g/100g-H$_2$O). Adjustments of PEG solution were based on water potentials of 0.0, -0.5, -1.1 and -1.5 MPa at room temperature. Observations were made at 3, 6, 9 and 12 days for 15$^{\circ}C$ and 1, 2, 3 and 4 days for 30$^{\circ}C$. Fifteen seeds of each cultivar were placed on Whatman No. 1 (9cm) filter paper in plastic pertridishes, and adjusted to 15 ml of the proper PEG solution supplemented with 0.2 percent thiram using automatic syringe. The dishes were covered with cap. The seeds were germinated at a continuous temperature of 15 or 30$^{\circ}C$ under dark conditions for programmed period. Seedling moisture content and seedling length of eight soybean cultivars decreased as PEG concentration increased both at 15 and 30$^{\circ}C$. Cultivar differences in seedling moisture content and seedling length were found among eight soybean cultivars at temperatures of 15 and 30$^{\circ}C$. Larger sized cultivar absorbed more moisture than samller sized cultivar. However, reverse results were obtained on the seedling moisture content of each of eight soybean cultivars. Cultiver Hill and Paldalkong showed greater seedling length than the other six cultivars from 20 to 30g/100g water of PEG concentrations both at 15 and 30$^{\circ}C$. The results of this study indicated that germination test of soybean seeds in aqueous solutions of PEG has potential for screening soybean cultivars for improved emergence during moisture stress.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.181-190
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• 2018

This study was conducted to investigate the occurrence of an acetolactate synthase (ALS) inhibiting herbicide resistant weed on paddy at 289 sites by soil sampling in Chungcheongbuk-Do of Republic of Korea from February to April in 2017. The most dominant weed was Monochoria vaginalis and Echinochloa oryzicola on each city and county. ALS inhibiting herbicide resistant ratio and occurrence area were 80.6% and 28,272 ha, respectively, in Chungcheongbuk-Do which was 3 times than in 5 years ago. The herbicide resistant ratio, Okcheon-Gun was the highest at 93.8%, Chungju-Si, Boeun-Gun, Yeongdong-Gun, Jincheon-Gun and Geosan-Gun were over 80%, Cheongju-Si, Eumseong-Gun and Jeungpyeong-Gun were over 70%. The herbicide resistant area, Cheongju-Si had the largest at 6,957 ha, Chungju-Si was 4,277 ha, Jincheon-Gun and Boeun-Gun was 3,536 ha and 3,282 ha, respectively. By weed, ALS inhibiting herbicide resistant ratio and occurrence area, Monochoria vaginalis was 49%, 17,646 ha, Echinochloa oryzicola 44%, 15,617 ha, Schoenoplectiella juncoides 29%, 10,377 ha, respectively. In all cities and counties of Chungcheongbuk-Do, Monochoria vaginalis and Echinochloa oryzicola are more than 40% resistant to ALS inhibiting herbicides, intensive management is required. The use of sulfonylurea herbicides is increasing, most farmers use herbicides 10 days after transplanting, so management after transplanting is necessary and the occurrence of herbicide resistant weeds should be reduced by alternating herbicide application every year.