• Archives of Pharmacal Research
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• v.29 no.10
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• pp.890-897
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• 2006

In inflammatory responses, induction of cytokines and other immune regulator genes in macrophages by pathogen-associated signal such as lipopolysaccharide (LPS) plays a crucial role. In this study, the gene expression profile changes by LPS treatment in the macrophage/monocyte lineage cell line RAW264.7 was investigated. A 60-mer oligonucleotide microarray of which probes target 32381 mouse genes was used. A reverse transcription-in vitro translation labeling protocol and a chemileuminescence detection system were employed. The mRNA expression levels in RAW264.7 cells treated for 6 h with LPS and the control vehicle were compared. 747 genes were up-regulated and 523 genes were down-regulated by more than 2 folds. 320 genes showing more than 4-fold change by LPS treatment were further classified for the biological process, molecular function, and signaling pathway. The biological process categories that showed high number of increased genes include the immunity and defense, the nucleic acid metabolism, the protein metabolism and modification, and the signal transduction process. The chemokine-cytokine signaling, interleukin signaling, Toll receptor signaling, and apoptosis signaling pathways involved high number of genes differentially expressed in response to LPS. These expression profile data provide more comprehensive information on LPS-target genes in RAW264.7 cells, which will be useful in comparing gene expression changes induced by extracts and compounds from anti-inflammatory medicinal herbs.

• International Journal of Oral Biology
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• v.34 no.1
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• pp.37-42
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• 2009

The attachment and adhesion of RAW 264.7 and MC3T3-E1 cells to titanium (Ti) discs with various degrees of roughness was investigated. The attachment, adhesion, and proliferation of these cells were evaluated after 4 hr, 24 hr and 7 day incubations. Both RAW 264.7 and MC3T3-E1 cells showed a time-dependant correlation between attachment and adhesion on the surface of the titanium discs. Both types of cells tended to have higher survival rate on these discs as the surface roughness increased. The percentage of adherent inflammatory RAW 264.7 cells was greater than MC3T3-E1 cells at 24 hr, but this was reversed at 7 days in culture. The morphology of osteoblastic MC3T3-E1 cells at 24 hr, determined using a surface emission microscope (SEM), appeared flattened and spread out while inflammatory RAW 264.7 cells were predominantly spherical in shape. The adhesion of both cell types on the titanium discs was dependant on the levels of fibronectin adsorbed on the disc surface, indicating that serum constituents modulate the efficient adhesion of these cells. Our data indicate that the cellular response to the titanium surface is dependent on the types of cells, surface roughness and serum constituents.

• Journal of Microbiology and Biotechnology
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• v.32 no.2
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• pp.141-148
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• 2022

Many bone diseases such as osteolysis, osteomyelitis, and septic arthritis are caused by gram-negative bacterial infection, and lipopolysaccharide (LPS), a bacterial product, plays an essential role in this process. Drugs that inhibit LPS-induced osteoclastogenesis are urgently needed to prevent bone destruction in infective bone diseases. Marein, a major bioactive compound of Coreopsis tinctoria, possesses anti-oxidative, anti-inflammatory, anti-hypertensive, anti-hyperlipidemic, and anti-diabetic effects. In this study, we measured the effect of marein on RAW264.7 cells by CCK-8 assay and used TRAP staining to determine osteoclastogenesis. The levels of osteoclast-related genes and NF-κB-related proteins were then analyzed by western blot, and the levels of pro-inflammatory cytokines were quantified by ELISA. Our results showed that marein inhibited LPS-induced osteoclast formation by osteoclast precursor RAW264.7 cells. The effect of marein was related to its inhibitory function on expressions of pro-inflammatory cytokines and osteoclast-related genes containing RANK, TRAF6, MMP-9, CK, and CAII. Additionally, marein leads to markedly inhibited NF-κB signaling pathway activation in LPS-induced RAW264.7 cells. Concurrently, when the NF-κB signaling pathway was inhibited, osteoclast formation and pro-inflammatory cytokine expression were decreased. Collectively, marein could inhibit LPS-induced osteoclast formation in RAW264.7 cells via regulating the NF-κB signaling pathway. Our data demonstrate that marein might be a potential drug for bacteria-induced bone destruction disease. Our findings provide new insights into LPS-induced bone disease.

• Journal of Conservation Science
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• v.38 no.4
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• pp.301-313
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• 2022

We made a Korean white porcelain or Joseon Baekja jar and based on the raw materials used and reproductions of each stage, we aimed to compare and analyze the physicochemical changes of the raw materials such as clay at each manufacturing stage, as well as identify the characteristics and correlations. Although the basic main components of clay and glaze material are similar, their texture becomes denser in the process of bisque firing pottery (Chobeol-pyeon) and glaze firing pottery (Jaebeol-pyeon), and we confirmed that in addition to the tendency of increasing vitrification, low-temperature minerals such as mica and illite gradually disappeared, while high-temperature minerals such as cristobalite were newly created. This phenomenon has also been verified by the rapid decrease in absorption rate while the change in specific gravity was small. In addition, the color was greatly affected by the firing atmosphere, and the yellow-red chromaticity of the raw materials was higher during bisque firing but showed a rapidly decreasing characteristic during glaze firing. The value of magnetic susceptibility, which is related to iron (Fe) component, showed a tendency to decrease in glaze firing pottery. CT images were confirmed as a method that can indirectly estimate the change in the material properties of the object step-by-step for the entire object. In conclusion, the study of manufacturing stages of reproduction can provide basic data for scientific research on the estimation of porcelain and pottery making technology and changes in raw materials.

• Journal of Korean Society for Geospatial Information Science
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• v.19 no.1
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• pp.79-86
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• 2011

Recently, aerial laser scanning technology has received full attention in constructing DEM(Digital Elevation Model). It is well known that the quality of DEM is mostly influenced by the accuracy of DTD(Digital Terrain Data) extracted from LiDAR(Light Detection And Ranging) raw data. However, there are always misclassified data in the DTD generated by automatic filtering process due to the limitation of automatic filtering algorithm and intrinsic property of LiDAR raw data. In order to eliminate the misclassified data, a manual filtering process is performed right after automatic filtering process. In this study, an algorithm that detects automatically possible misclassified data included in the DTD from automatic filtering process is proposed, which will reduce the load of manual filtering process. The algorithm runs on 2D grid data structure and makes use of several parameters such as 'Slope Angle', 'Slope DeltaH' and 'NNMaxDH(Nearest Neighbor Max Delta Height)'. The experimental results show that the proposed algorithm quite well detected the misclassified data regardless of the terrain type and LiDAR point density.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.891-897
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• 2022

In big data analysis, raw text data mostly exists in various unstructured data forms, so it becomes a structured data form that can be analyzed only after undergoing heuristic pre-processing and computer post-processing cleansing. Therefore, in this study, unnecessary elements are purified through pre-processing of the collected raw data in order to apply the wordcloud of R program, which is one of the text data analysis techniques, and stopwords are removed in the post-processing process. Then, a case study of wordcloud analysis was conducted, which calculates the frequency of occurrence of words and expresses words with high frequency as key issues. In this study, to improve the problems of the "nested stopword source code" method, which is the existing stopword processing method, using the word cloud technique of R, we propose the use of "general stopword corpus" and "user-defined stopword corpus" and conduct case analysis. The advantages and disadvantages of the proposed "unstructured data cleansing process model" are comparatively verified and presented, and the practical application of word cloud visualization analysis using the "proposed external corpus cleansing technique" is presented.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.6
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• pp.135-140
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• 2015

The Cyber-Physical Systems (CPS) complexly perform modeling and simulation (M&S) for the various embedded systems. In this case, due to use diverse formatted models, we suggest to apply with the SEDRIS to systematically manage the different formatted data on M&S. The SEDRIS can reduce time and cost with reusing and interoperating environment data developed in the specific domain. To do this, we should input the data transformed the height map for terrain representation in a simulator into raster data of SEDRIS for which interoperate between the existed simulator and the SEDRIS. To solve the problem, we propose the transformation method to transfer the polygon data from RAW file used in terrain representation. With the proposed method, we can provide two advantages. First, it can possibly express the environment data into SEDRIS. Second, we can see the terrain like an image file through a viewer. Therefore, even non-expert easily constructs the terrain environment data.

• Journal of Satellite, Information and Communications
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• v.1 no.2
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• pp.25-31
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• 2006

The COMS (Communication, Ocean, and Meteorological Satellite) performing meteorological and ocean monitoring and providing communication service with meteorological, ocean and Ka-band payload in the geostationary orbit includes MODCS (Meteorological and Ocean Data Communication Subsystem) which provides transmitting the raw data collected by meteorological payload called MI (Meteorological Imager) and ocean payload named GOCI (Geostationary Ocean Color Imager) to the ground station and relaying the meteorological data processed on the ground to the end-user stations. MODCS comprises of two channels: SD channel which formats the raw data according to CCSDS recommendation, amplifies and transmits its signal to the ground station; MPDR channel which relays to the end-user stations the ground-processed meteorological data in the data format of LRIT/HRIT recommended by CGMS. This paper constructs the architecture of MODCS for transmitting and relating the observed data, and investigates that the key performance parameters have the required margin through the preliminary performance analyses.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.745-750
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• 2021

In Big data visualization analysis of unstructured text data, raw data is mostly large-capacity, and analysis techniques cannot be applied without cleansing it unstructured. Therefore, from the collected raw data, unnecessary data is removed through the first heuristic cleansing process and Stopwords are removed through the second machine cleansing process. Then, the frequency of the vocabulary is calculated, visualized using the word cloud technique, and key issues are extracted and informationalized, and the results are analyzed. In this study, we propose a new Stopword cleansing technique using an external Stopword set (DB) in Python word cloud, and derive the problems and effectiveness of this technique through practical case analysis. And, through this verification result, the utility of the practical application of word cloud analysis applying the proposed cleansing technique is presented.

• Proceedings of the Korean Statistical Society Conference
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• 2002.05a
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• pp.175-181
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• 2002

cDNA microarray experiments permit us to investigate the expression levels of thousands of genes simultaneously and to make it easy to compare gene expression from different populations. However, researchers are asked to be cautious in interpreting the results because of the unexpected sources of variation such as systematic errors from the microarrayer and the difference of cDNA dye intensity. And the scanner itself calculates both of mean and median of the signal and background pixels, so it follows a selection which raw data will be used in analysis. In this paper, we compare the results in each case of using mean and median from the raw data and normalization methods in reducing the systematic errors with arm's skin cells of old and young males. Using median is preferable to mean because the distribution of the test statistic (t-statistic) from the median is more close to normal distribution than that from mean. Scaled print tip normalization is better than global or lowess normalization due to the distribution of the test-statistic.