• 한국습지학회지
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• 제11권2호
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• pp.17-28
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• 2009

광주광역시 도심 영산강 수계의 관속식물은 91과 265속 386종 49변종 및 2품종으로 총 437분류군이 확인되었다. 수생식물 중 정수식물은 16분류군, 부엽식물은 10분류군, 침수식물은 10분류군, 부유식물은 3분류군 그리고 습생식물은 14분류군으로 총 53분류군이었다. 희귀 및 멸종위기종은 자라풀, 가시연꽃, 낙지다리, 왕벚나무, 좀어리연꽃 등 5분류군이었다. 특정식물종 I급종은 10분류군, II급종 2분류군, III급종 2분류군, IV급종 3분류군 그리고 V급종 5분류군이 확인되었다. 귀화식물은 15과 44속 59종 3변종으로 총 62분류군이 확인되었고 귀화율은 14.19%였다. 환경부 생태계 교란종은 돼지풀, 물참새피 및 도깨비가지 등이었다.

• 농업생명과학연구
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• 제45권3호
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• pp.9-18
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• 2011

본 연구는 경상북도 봉화군 비룡산 일대의 신갈나무림을 대상으로 하층수관층에 출현하는 식물 다양성을 파악하기 위해 수행되었다. 종-면적 곡선의 분석결과 신갈나무림 내의 식물다양성의 분석에 적정 크기의 방형구를 설치한 것으로 판단되었다. 5개 조사구 전체의 소산식물은 47과 80속 91종 11변종 2아종 3품종으로 총 107분류군이다. 본 조사에서 기록한 식물종 중 우리나라의 특산식물은 지리대사초, 고광나무, 병꽃나무로 총 3종이다. 한편 희귀식물로는 꼬리진달래 1종 뿐이다. 생육형은 광엽초본에 해당하는 식물종이 가장 높게 나타났고, 관목, 교목 순이었다.

• Mycobiology
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• 제49권3호
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• pp.223-234
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• 2021

Itajahya rosea was found growing in association with Leucaena leucocephala plants at Savitribai Phule Pune University campus in India. The species identity was confirmed by phylogenetic analysis based on ITS and LSU regions of rDNA, wherein, our fugus was placed along with I. rosea in phylogenetic tree. It represents first record of I. rosea from India. Frequent visitation by Drosophila species on I. rosea fruiting body particularly on gleba was observed. The Drosophila got attracted to the detached gleba under the laboratory conditions and even sometimes, they prefer to sit over the gleba as compare to their food banana. It suggested that I. rosea gleba or pseudostipe produces some compounds for attraction and feeding behavior of Drosophila species. Therefore, we characterized the volatile attractants produced by gleba and pseudostipe of I. rosea by GC-MS analysis. Nineteen compounds were identified from gleba while nine compounds were recovered from the pseudostipe. Out of them, blends of three abundant odor producing volatile compounds were reported namely, Hexadecane, Pentadecane and Nonadecane, which are responsible for attraction of Drosophila toward the gleba. Three fatty acids namely 9,12-octadecadienoic acid (Z,Z), hexadecanoic acid and benzoic acid ethyl ester produced are served as an appetitive signal through olfactory response of Drosophila, so the flies were feed on the gleba. Two pheromones' compounds, heneicosane and (+)-(5S,9S)-5,9-dimethylpentadecane, were also reported in pseudostipe and gleba, respectively, which play a role in Drosophila for breeding. Our study highlights an intriguing chemical ecology of fungus-Drosophila interaction.

• Parasites, Hosts and Diseases
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• 제56권6호
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• pp.603-607
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• 2018

This study was carried out to determine the pathogen-causing diarrhoea in sheep Ovis aries in the Qinghai Tibetan Plateau Area, China. A trophozoite was identified as species of ciliate alveolates infecting the sheep based on morphological characteristics examined by microscope. It was mostly spherical, colourless and transparent, with many vesicles. Macronucleus and contractile vacuoles could not be distinguished. Size of the trophozoite was $80-180{\times}70-150{\mu}m$ and its surface was covered with cilia. Molecular analysis based on sequences of 18S rRNA and ITS genes confirmed the ciliate species as Balantidium coli. According to the literature, there have been many epidemiological investigations of B. coli infection in pigs, monkeys and humans. To our knowledge, this was the first report of B. coli infections in sheep in the Qinghai Tibetan Plateau Area of China, or eleswhere around the world. Importantly, the sheep case was rare but raised our concern that B. coli may spread across species and expand its host range.

• Parasites, Hosts and Diseases
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• 제60권2호
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• pp.143-147
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• 2022

Acanthamoeba keratitis (AK) is a rare ocular disease, but it is a painful and sight-threatening infectious disease. Early diagnosis and adequate treatment are necessary to prevent serious complications. While AK is frequently diagnosis via several PCR assays or Acanthamoeba-specific antibodies, a more specific and effective diagnostic method is required. This study described the production of a polyclonal peptide antibody against the periplasmic binding protein (PBP) of A. castellanii and investigated its diagnostic potential. Western blot analysis showed that the PBP antibody specifically reacted with the cell lysates of A. castellanii. However, the PBP antibody did not interact with human corneal epithelial (HCE) cells and the other 3 major causative agents of keratitis. Immunocytochemistry (ICC) results revealed the specific detection of A. castellanii trophozoites and cysts by PBP antibodies when A. castellanii were co-cultured with HCE cells. PBP antibody specificity was further confirmed by co-culture of A. castellanii trophozoites with F. solani, S. aureus, and P. aeruginosa via ICC. The PBP antibody specifically reacted with the trophozoites and cysts of A. polyphaga, A. hatchetti, A. culbertsoni, A. royreba, and A. healyi, thus demonstrated its genus-specific nature. These results showed that the PBP polyclonal peptide antibody of A. castellanii could specifically detect several species of Acanthamoeba, contributing to the development of an effective antibody-based AK diagnostics.

• 인간식물환경학회지
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• 제24권6호
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• pp.619-628
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• 2021

Background and objective: Native to the limestone zones of the Korean Peninsula, Zabelia tyaihyonii is a popular plant for landscaping. As it is now classified as a rare species, the conservation of its genetic resources is necessary. Methods: In this study, which aimed to understand the morphological variation of Z. tyaihyonii, 18 characteristics of Z. tyaihyonii from five habitats were examined. Results: Of these 18 characteristics, 16 characteristics showed significant differences among sites, and the coefficient of variation ranged from 5.4% (for corolla lobe number) to 31.3% (for flower number). Notable variations were observed in the size of flower and calyx lobe. When the corolla length and calyx lobe length were used as the classification key of Z. tyaihyonii, the sites were divided into those with small, intermediate, and large values. Hair was observed on the filament of all samples, a finding which conflicts with an earlier report. Rather than classifying Z. tyaihyonii into different species on the basis of corolla length (COL) and calyx lobe length (CALL) values, we recommend modifying the species description to incorporate the variation in these characteristics of interest. Principal component analysis results showed that the first main component was highly correlated with the traits related to the size of the calyx lobe (length: 0.819, width: 0.758), and the second main component was highly correlated with the traits related with the size of the inflorescence (length: 0.790, width: 0.626). Conclusion: Several notable variations were identified among the characteristics related to inflorescence and calyx lobe. There is little genetic exchange among groups, or each group is influenced by micro environmental factors, because sites that are located nearby. In addition, the difference between COL and CALL, which is used as the classification key for Z. tyaihyonii, was divided into small group, large group, and intermediate group, regardless of the sites' geographical distance.

• International Journal of Industrial Entomology and Biomaterials
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• 제46권2호
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• pp.41-54
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• 2023

The blue-tailed damselfly, Ischnura elegans Van der Linden, 1820 (Odonata: Coenagrionidae), is a climate-sensitive indicator species in South Korea. In this study, we sequenced the complete mitochondrial genome (mitogenome) of I. elegans collected from South Korea for subsequent population genetic analysis, particularly to trace population movements in response to climate change. The 15,963 base pair (bp)-long complete mitogenome of I. elegans has typical sets of genes including a major non-coding region (the A+T-rich region), and an arrangement identical to that observed in ancestral insect species. The ATP6, ND3 and ND1 genes have the TTG start codon, which, although rare, is the canonical start codon for animal mitochondrial tRNA. The A/T content was 71.4% in protein-coding genes, 72.1% in tRNAs, 72.9% in the whole genome, 74.7% in srRNA, 75.3% in lrRNA, and 83.8% in the A+T-rich region. The A+T-rich region is unusually long (1,196 bp) and contains two subunits (192 bp and 176-165 bp), each of which is tandemly triplicated and surrounded by non-repeat sequences. Comparison of the sequence divergence among available mitogenomes of I. elegans, including the one from the current study, revealed ND2 as the most variable gene, followed by COII and COI, suggesting that ND2 should be targeted first in subsequent population-level studies. Phylogenetic reconstruction based on all available mitogenome sequences of Coenagrionidae showed a strong sister relationship between I. elegans and I. senegalensis.

• 한국습지학회지
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• 제26권1호
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• pp.92-113
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• 2024

본 연구는 김해 화포천습지 보호지역에 분포하는 관속식물상의 특성을 조사하여 습지생태계 보전과 관리를 위한 기초정보를 제공하는데 목적이 있다. 관속식물상은 81과 221속 315종 9아중 12변종 2품종 등 339분류군으로 요약되었다. 희귀식물은 쥐방울덩굴, 자라풀 등 7분류군이며, 식물구계학적 특정종은 32분류군이고, 침입외래식물은 66분류군이며, 생태계교란종은 11분류군이었다. 수생식물은 29분류군이며, 정수식물은 16분류군, 침수식물은 3분류군, 부유식물은 4분류군, 부엽식물은 6분류군으로 나타났다.

• 한국환경생태학회지
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• 제11권3호
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• pp.366-386
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• 1997

이 연구는 명지산의 식물상을 파악하여 중부 이북 지역 식물상의 기초 자료를 제공하고 희귀 식물 분포 특성에 따라 명지산 지역의 희귀 식물 보존 전략 수립에 화용하기 위해 이루어졌다. 조사는 1991년부터 1997년 9월까지 21차례에 걸쳐 명지산 일대에서 이루어졌으며 그 결과를 요약하면 다음과 같다. 1. 명지산은 해발 600m 정도를 경계로 아래쪽은 잣나무 및 낙엽송 조림지로 구성되어 있으며, 그 위쪽으로는 신갈나무를 우점으로하여 다양한 수종들이 혼생하는 낙엽활엽수림을 이루고 있고, 바람꽃류 및 금낭화 대군락, 흰노루오줌, 흰도라지모싯대 등의 식물이 특징적인 종이었다. 2. 본 조사를 통해 확인된 명지산의 총 소산식물은 101과 359속 576종 90변종 11품종의 총 677종류로 나타났으며, 그 가운데 특산 식물은 19과 31속 32종류, 귀화식물의 27종류로 특히 귀화 식물의 67%는 최근 출현한 종들이었다. 3. 명지산에는 산림청 지정 희귀 및 멸종위기식물 가운데 총 26종류의 희귀 및 멸종위기식물이 나타났으며 특히 광릉요강꽃, 애기송이풀, 깽깽이풀, 개족도리 등의 분포는 특기할 만하였고, 따라서 이 지역 희귀식물에 대한 조합적인 보존 전략의 수립이 필요하다.

• 대한수의학회지
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• 제43권3호
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• pp.339-347
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• 2003

The regional distribution and relative frequency of some endocrine cells in the pancreas of the Korean aucha perch, Coreoperca herzi Herzenstein belonging to the family Serranidae in order Perciformis, were observed using specific mammalian antisera against serotonin, insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP) by peroxidase antiperoxidase (PAP) method. The pancreas was divided into four portions (principal and secondary islets, exocrine and pancreatic duct regions). In addition, the pancreatic islet regions were further subdivided into three regions (central, mantle and peripheral regions). Spherical to spindle or occasionally round to oval immunoreactive (IR) cells were demonstrated in the pancreatic islets and exoccrine portions, but no cells were detected in the pancreatic duct portions. In the principal islets, serotonin-IR cells were not detected but most of insulin-IR cells were located in the central regions and they were also demonstrated in the mantle and peripheral regions in moderate and rare frequencies, respectively. Glucagon- and hPP-IR cells were mainly situated in the mantle regions but the cells were also demonstrated in the peripheral regions in relatively lower frequency. Somatostatin-IR cells were evenly distributed in the central and mantle regions in a few frequency and cells were also demonstrated in the peripheral regions in rare frequency. Cell clusters were consisted of hPP-IR cells that were situated in the peripheral to mantle regions. In the secondary islet portions, serotonin-IR cells were randomly distributed throughout the whole pancreatic islet regions but lower frequency was detected in the peripheral regions compared to that in central and mantle regions where cells were detected in a few frequency, respectively. Insulin-IR cells were restricted to the central regions in numerous frequency and glucagon-IR cells were evenly distributed in the mantle and peripheral regions in moderate frequencies, respectively. Somatostatin-IR cells were observed in the central and mantle regions in moderate and a few frequencies, respectively. In addition, hPP-IR cells showed similar distributional patterns to those of glucagon-IR cells except cells were also located in the central regions in rare frequency. In the exocrine portions, only glucagon- and hPP-IR cells were demonstrated in rare and a few frequencies, respectively. In conclusion, the regional distribution and relative frequency of pancreatic endocrine cells of the Korean aucha perch showed general patterns, which were observed in other teleost. However, some species-dependent different distributional patterns and/or relative frequencies were also demonstrated especially to serotonin-IR cells. In pancreas of the Korean aucha perch, insulin-IR cells were the most predominant cell type followed by glucagon-, somatostatin-, hPP- and serotonin-IR cells.