• Journal of Wetlands Research
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• v.11 no.2
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• pp.17-28
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• 2009

The investigated Varcular plants in Youngsan River Streams of Downtown in Gwangju Metropolitan City consist of total 437 taxa: 2 forms, 49 varieties, 386 species, 265 genera, 91 families. Among 53 taxa of hydrophytes, emerged plants were 16taxa, floating-leaved plants were 10taxa, suvmerged plants were 10taxa, and free-floating plants were 3taxa and swamp plants were 14taxa. Based on the list of Rare and Endangered plants, 5taxa were recorded such as Hydrocharis dubia, Euryale Ferox, Penthorum chinense, Prunus yedoensis, Nymphoides coreana. And Korean endemic Plants were appeared as 5taxa: Poa annua, Forsythia koreana, Paulownia coreana, Galium koreanum, Aster koraiensis. From the specific plant species sorted by classes, class I has 10taxa, class II has 2taxa, class III has 2taxa, class IV has 3taxa, class V has 5taxa. Naturalized plant were listed as 62taxa: 15families, 44genera, 59species, 3varieties, and naturalization index was 14.19%. The ecosystem disturbance plants assigned by the Ministry of Environment, 3taxa were recorded: Paspalum distichum, Ambrosia artemisiifolia var. elatior, Solanum carilinense.

• Journal of agriculture & life science
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• v.45 no.3
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• pp.9-18
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• 2011

This study was performed to investigate species diversity of understory in Mt. Biryong. The quadrat size was installed based on the result of analysis of Species-Area Curve. The vascular plants were enlisted 107 taxa: 47 families, 80 genera, 91 species, 11 varieties, 2 subspecies, and 3 forms. The three taxa such as Carex okamotoi, Philadelphus schrenkii var. schrenkii, and Weigela subsessilis were enlisted from the surveyed sites as the Korean endemic plants. Due to the list from the Korea Forestry Administration, Rhododendron micranthum was enlisted as the rare plant. The growth from was categorized followed by forbs, shrubs and trees, respectively.

• Mycobiology
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• v.49 no.3
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• pp.223-234
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• 2021

Itajahya rosea was found growing in association with Leucaena leucocephala plants at Savitribai Phule Pune University campus in India. The species identity was confirmed by phylogenetic analysis based on ITS and LSU regions of rDNA, wherein, our fugus was placed along with I. rosea in phylogenetic tree. It represents first record of I. rosea from India. Frequent visitation by Drosophila species on I. rosea fruiting body particularly on gleba was observed. The Drosophila got attracted to the detached gleba under the laboratory conditions and even sometimes, they prefer to sit over the gleba as compare to their food banana. It suggested that I. rosea gleba or pseudostipe produces some compounds for attraction and feeding behavior of Drosophila species. Therefore, we characterized the volatile attractants produced by gleba and pseudostipe of I. rosea by GC-MS analysis. Nineteen compounds were identified from gleba while nine compounds were recovered from the pseudostipe. Out of them, blends of three abundant odor producing volatile compounds were reported namely, Hexadecane, Pentadecane and Nonadecane, which are responsible for attraction of Drosophila toward the gleba. Three fatty acids namely 9,12-octadecadienoic acid (Z,Z), hexadecanoic acid and benzoic acid ethyl ester produced are served as an appetitive signal through olfactory response of Drosophila, so the flies were feed on the gleba. Two pheromones' compounds, heneicosane and (+)-(5S,9S)-5,9-dimethylpentadecane, were also reported in pseudostipe and gleba, respectively, which play a role in Drosophila for breeding. Our study highlights an intriguing chemical ecology of fungus-Drosophila interaction.

• Parasites, Hosts and Diseases
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• v.56 no.6
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• pp.603-607
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• 2018

This study was carried out to determine the pathogen-causing diarrhoea in sheep Ovis aries in the Qinghai Tibetan Plateau Area, China. A trophozoite was identified as species of ciliate alveolates infecting the sheep based on morphological characteristics examined by microscope. It was mostly spherical, colourless and transparent, with many vesicles. Macronucleus and contractile vacuoles could not be distinguished. Size of the trophozoite was $80-180{\times}70-150{\mu}m$ and its surface was covered with cilia. Molecular analysis based on sequences of 18S rRNA and ITS genes confirmed the ciliate species as Balantidium coli. According to the literature, there have been many epidemiological investigations of B. coli infection in pigs, monkeys and humans. To our knowledge, this was the first report of B. coli infections in sheep in the Qinghai Tibetan Plateau Area of China, or eleswhere around the world. Importantly, the sheep case was rare but raised our concern that B. coli may spread across species and expand its host range.

• Parasites, Hosts and Diseases
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• v.60 no.2
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• pp.143-147
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• 2022

Acanthamoeba keratitis (AK) is a rare ocular disease, but it is a painful and sight-threatening infectious disease. Early diagnosis and adequate treatment are necessary to prevent serious complications. While AK is frequently diagnosis via several PCR assays or Acanthamoeba-specific antibodies, a more specific and effective diagnostic method is required. This study described the production of a polyclonal peptide antibody against the periplasmic binding protein (PBP) of A. castellanii and investigated its diagnostic potential. Western blot analysis showed that the PBP antibody specifically reacted with the cell lysates of A. castellanii. However, the PBP antibody did not interact with human corneal epithelial (HCE) cells and the other 3 major causative agents of keratitis. Immunocytochemistry (ICC) results revealed the specific detection of A. castellanii trophozoites and cysts by PBP antibodies when A. castellanii were co-cultured with HCE cells. PBP antibody specificity was further confirmed by co-culture of A. castellanii trophozoites with F. solani, S. aureus, and P. aeruginosa via ICC. The PBP antibody specifically reacted with the trophozoites and cysts of A. polyphaga, A. hatchetti, A. culbertsoni, A. royreba, and A. healyi, thus demonstrated its genus-specific nature. These results showed that the PBP polyclonal peptide antibody of A. castellanii could specifically detect several species of Acanthamoeba, contributing to the development of an effective antibody-based AK diagnostics.

• Journal of People, Plants, and Environment
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• v.24 no.6
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• pp.619-628
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• 2021

Background and objective: Native to the limestone zones of the Korean Peninsula, Zabelia tyaihyonii is a popular plant for landscaping. As it is now classified as a rare species, the conservation of its genetic resources is necessary. Methods: In this study, which aimed to understand the morphological variation of Z. tyaihyonii, 18 characteristics of Z. tyaihyonii from five habitats were examined. Results: Of these 18 characteristics, 16 characteristics showed significant differences among sites, and the coefficient of variation ranged from 5.4% (for corolla lobe number) to 31.3% (for flower number). Notable variations were observed in the size of flower and calyx lobe. When the corolla length and calyx lobe length were used as the classification key of Z. tyaihyonii, the sites were divided into those with small, intermediate, and large values. Hair was observed on the filament of all samples, a finding which conflicts with an earlier report. Rather than classifying Z. tyaihyonii into different species on the basis of corolla length (COL) and calyx lobe length (CALL) values, we recommend modifying the species description to incorporate the variation in these characteristics of interest. Principal component analysis results showed that the first main component was highly correlated with the traits related to the size of the calyx lobe (length: 0.819, width: 0.758), and the second main component was highly correlated with the traits related with the size of the inflorescence (length: 0.790, width: 0.626). Conclusion: Several notable variations were identified among the characteristics related to inflorescence and calyx lobe. There is little genetic exchange among groups, or each group is influenced by micro environmental factors, because sites that are located nearby. In addition, the difference between COL and CALL, which is used as the classification key for Z. tyaihyonii, was divided into small group, large group, and intermediate group, regardless of the sites' geographical distance.

• International Journal of Industrial Entomology and Biomaterials
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• v.46 no.2
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• pp.41-54
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• 2023

The blue-tailed damselfly, Ischnura elegans Van der Linden, 1820 (Odonata: Coenagrionidae), is a climate-sensitive indicator species in South Korea. In this study, we sequenced the complete mitochondrial genome (mitogenome) of I. elegans collected from South Korea for subsequent population genetic analysis, particularly to trace population movements in response to climate change. The 15,963 base pair (bp)-long complete mitogenome of I. elegans has typical sets of genes including a major non-coding region (the A+T-rich region), and an arrangement identical to that observed in ancestral insect species. The ATP6, ND3 and ND1 genes have the TTG start codon, which, although rare, is the canonical start codon for animal mitochondrial tRNA. The A/T content was 71.4% in protein-coding genes, 72.1% in tRNAs, 72.9% in the whole genome, 74.7% in srRNA, 75.3% in lrRNA, and 83.8% in the A+T-rich region. The A+T-rich region is unusually long (1,196 bp) and contains two subunits (192 bp and 176-165 bp), each of which is tandemly triplicated and surrounded by non-repeat sequences. Comparison of the sequence divergence among available mitogenomes of I. elegans, including the one from the current study, revealed ND2 as the most variable gene, followed by COII and COI, suggesting that ND2 should be targeted first in subsequent population-level studies. Phylogenetic reconstruction based on all available mitogenome sequences of Coenagrionidae showed a strong sister relationship between I. elegans and I. senegalensis.

• Journal of Wetlands Research
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• v.26 no.1
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• pp.92-113
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• 2024

The Purpose of this study were to present the basic data for conservation and management of wetland ecosystem by surveying the characteristics of vascular plants distributed in Hwapocheon wetland protected area. The results are as follows. The numbers of vascular plants were summrized as 339 taxa including 81 familices, 221 genera, 315 species, 9 subspecies and 12 varieties and 2 forms. The rare plants were 7 taxa including Aristolochia contorta, Hydrocharis dubia and so on. The floristic target species were 32 taxa including 17 taxa of grade I, 7 taxa of grade II, 4 taxa of grade III, 2 taxa of grade IV and 2 taxa of grade V. The invasive alien plants were 66 Rumex crispus, Oenothera biennis, Veronica arvensis and so on. The ecosystem disturbing species were 11 taxa including Humulus scandens, Ambrosia artemisiifolia, Ambrosia trifida, Lactuca seriola, Symphyotrichum pilosum and so on. The hydrophytes were 29 taxa including 16 taxa of emergent plants, 3 taxa of submerged plants, 4 taxon of free-floating plant 6 taxa of floation-leaved plants.

• Korean Journal of Environment and Ecology
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• v.11 no.3
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• pp.366-386
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• 1997

This study was conducted to investigate the flora of Mt. Myongji and also to find the distribution characteristecs of rare and endangered plants in this mountain. Field surveys were carride out 21 times from 1991 to 1997. Main results obtained from this study were as follows; 1. Mt. Myougji was covered with Pinus koraiensis and Sarix leptolepis stands at the altitude lower than 600m, and deciduous forests which were consisted of Quercus mongolica, Carpinus sp., Acer sp., Cornus sp., Fracinus sp., etc. occupied at the altitude higher than 600m. Dicentra spectabilis population and Anemone population were remardable, and Adenophora grandiflora and Astilbe chinensis var. davidii with white flowers were found. 2. The vascular plants in Mt. Myoungji were recorded as 677 taxa such as 19 families, 358 genera 576 species 90 varieties 11 forma. Among them. 19 families 31 genera 32 taxa were endemic plants and 27 taxa were naturalized plants. 3. 26 taxa of rare and endangered plants protected by Forestry Administration were found in Mt. Myonfji. Among them, Asarum maculatum. Hanabusaya asiatica, Lilum cernum. Jeffersonia dubia, and Cypripedium japonica populations could be regarded as very important in a biogeraphical aspect. So it is necessary to monitor this area in a long term and to establish the conservation strategies.

• Korean Journal of Veterinary Research
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• v.43 no.3
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• pp.339-347
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• 2003

The regional distribution and relative frequency of some endocrine cells in the pancreas of the Korean aucha perch, Coreoperca herzi Herzenstein belonging to the family Serranidae in order Perciformis, were observed using specific mammalian antisera against serotonin, insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP) by peroxidase antiperoxidase (PAP) method. The pancreas was divided into four portions (principal and secondary islets, exocrine and pancreatic duct regions). In addition, the pancreatic islet regions were further subdivided into three regions (central, mantle and peripheral regions). Spherical to spindle or occasionally round to oval immunoreactive (IR) cells were demonstrated in the pancreatic islets and exoccrine portions, but no cells were detected in the pancreatic duct portions. In the principal islets, serotonin-IR cells were not detected but most of insulin-IR cells were located in the central regions and they were also demonstrated in the mantle and peripheral regions in moderate and rare frequencies, respectively. Glucagon- and hPP-IR cells were mainly situated in the mantle regions but the cells were also demonstrated in the peripheral regions in relatively lower frequency. Somatostatin-IR cells were evenly distributed in the central and mantle regions in a few frequency and cells were also demonstrated in the peripheral regions in rare frequency. Cell clusters were consisted of hPP-IR cells that were situated in the peripheral to mantle regions. In the secondary islet portions, serotonin-IR cells were randomly distributed throughout the whole pancreatic islet regions but lower frequency was detected in the peripheral regions compared to that in central and mantle regions where cells were detected in a few frequency, respectively. Insulin-IR cells were restricted to the central regions in numerous frequency and glucagon-IR cells were evenly distributed in the mantle and peripheral regions in moderate frequencies, respectively. Somatostatin-IR cells were observed in the central and mantle regions in moderate and a few frequencies, respectively. In addition, hPP-IR cells showed similar distributional patterns to those of glucagon-IR cells except cells were also located in the central regions in rare frequency. In the exocrine portions, only glucagon- and hPP-IR cells were demonstrated in rare and a few frequencies, respectively. In conclusion, the regional distribution and relative frequency of pancreatic endocrine cells of the Korean aucha perch showed general patterns, which were observed in other teleost. However, some species-dependent different distributional patterns and/or relative frequencies were also demonstrated especially to serotonin-IR cells. In pancreas of the Korean aucha perch, insulin-IR cells were the most predominant cell type followed by glucagon-, somatostatin-, hPP- and serotonin-IR cells.