• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.703-704
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• 2005

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.422-423
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• 2005

• Proceedings of the Korean Nuclear Society Conference
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• 2004.10a
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• pp.1373-1374
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• 2004

• Proceedings of the Korean Nuclear Society Conference
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• 2005.05a
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• pp.1144-1145
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• 2005

• Animal Bioscience
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• v.37 no.6
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• pp.1021-1030
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• 2024

Objective: R-loops are DNA:RNA triplex hybrids, and their metabolism is tightly regulated by transcriptional regulation, DNA damage response, and chromatin structure dynamics. R-loop homeostasis is dynamically regulated and closely associated with gene transcription in mouse zygotes. However, the factors responsible for regulating these dynamic changes in the R-loops of fertilized mouse eggs have not yet been investigated. This study examined the functions of candidate factors that interact with R-loops during zygotic gene activation. Methods: In this study, we used publicly available next-generation sequencing datasets, including low-input ribosome profiling analysis and polymerase II chromatin immunoprecipitation-sequencing (ChIP-seq), to identify potential regulators of R-loop dynamics in zygotes. These datasets were downloaded, reanalyzed, and compared with mass spectrometry data to identify candidate factors involved in regulating R-loop dynamics. To validate the functions of these candidate factors, we treated mouse zygotes with chemical inhibitors using in vitro fertilization. Immunofluorescence with an anti-R-loop antibody was then performed to quantify changes in R-loop metabolism. Results: We identified DEAD-box-5 (DDX5) and histone deacetylase-2 (HDAC2) as candidates that potentially regulate R-loop metabolism in oocytes, zygotes and two-cell embryos based on change of their gene translation. Our analysis revealed that the DDX5 inhibition of activity led to decreased R-loop accumulation in pronuclei, indicating its involvement in regulating R-loop dynamics. However, the inhibition of histone deacetylase-2 activity did not significantly affect R-loop levels in pronuclei. Conclusion: These findings suggest that dynamic changes in R-loops during mouse zygote development are likely regulated by RNA helicases, particularly DDX5, in conjunction with transcriptional processes. Our study provides compelling evidence for the involvement of these factors in regulating R-loop dynamics during early embryonic development.

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.701-702
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• 2005

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.13 no.8
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• pp.692-700
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• 2001

Flow boiling heat transfer coefficients(HTCs) of R22, R134a, R407C, and R410A were measured experimentally for a horizontal plain and a microfin tube. Experimental apparatus was composed of 3 main parts: a refrigerant loop, a water loop and a water-glycol loop. The test section in th refrigerant loop was made of a copper tube of 9.52 mm outer diameter and 1 m length for both tubes. The refrigerant was heated by passing hot water through an annulus surrounding the test section. Tests were performed at a fixed refrigerant saturation temperature of $5^{\circ}C$ with mass fluxes of 100~300 kg/$m^2$s. Test results showed that at similar mass flux the flow boiling HTCs of R134a were similar to those of R22 for both plain and microfin tube. HTCs of R407C were similar to those of R22 for a plain tube but lower than those of R2 by 25~48% for a microfin tube. And HTCs of R410A were higher than those of R2 by 20~63% for a plain tube and were similar to those of R22 for a microfin tube. In general, HTCs of a microfin tube were 1.8~5.7 times higher than those of a plain tube.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.16 no.5
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• pp.444-451
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• 2004

Flow condensation heat transfer coefficients (HTCs) of R22, R134a, R407C, and R410A were measured on horizontal plain and microfin tubes. The experimental apparatus was composed of three main parts; a refrigerant loop, a water loop and a water/glycol loop. The test section in the refrigerant loop was made of both a plain and a microfin copper tube of 6.0∼6.16 mm inside diameter and 1.0 m length. Refrigerants were cooled by passing cold water through an annulus surrounding the test section. Tests were performed at a fixed refrigerant saturation temperature of 4$0^{\circ}C$ with mass fluxes of 100, 200, and 300 kg/m2s. Test results showed that at similar mass flux the flow condensation HTCs of R134a were similar to those of R22 for both plain and microfin tubes. On the other hand, HTCs of R407C were lower than those of R22 by 4∼16% and 16∼42% for plain and microfin tubes respectively. And HTCs of R410A were similar to those of R22 for a plain tube but lower than those of R22 by 3∼9% for a microfin tube. Heat transfer enhancement factors of a microfin tube were 1.3∼1.9.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.14 no.2
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• pp.175-183
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• 2002

Flow Condensation heat transfer coefficients (HTCs) of Rl2, R22, R32, Rl23, Rl25, R134a, R142b were measured experimentally on a horizontal plain tube. The experi- mental apparatus was composed of three main parts; a refrigerant loop, a water loop and a water-glycol loop. The test section in a refrigerant loop was made of a copper tube of 8.8 mm inner diameter and 1000 mm length respectively. The refrigerant was cooled by passing cold water through an annulus surrounding the test section. All tests were performed at a filed refrigerant saturation temperature of 4$0^{\circ}C$ with mass fluxes of 100, 200, 300 kg/$m^2$s. The experimental result showed that flow condensation HTCs increase as the quality, mass flux, and latent heat of condensation increase. At the same mass flux, the HTCs of R32 and R142b were higher than those of R22 by 35~45% and 7~14% respectively while HTCs of R134a and Rl23 were similar to those of R22. On the other hand, HTCs of Rl25 and Rl2 were lower than those of R22 by 28 ~30% and 15 ~25% respectively Finally, a new correlation for flow condensation HTCs was developed by modifying Dobson and Chato's correlation with the latent heat of condensation considered. The correlaton showed an average deviation of 13.1% for all pure fluids data indicating an excellent agreement.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.14 no.8
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• pp.656-663
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• 2002

Flow condensation heat transfer coefficients (HTCs) of R22, R134a, R407C, and R410A were measured on horizontal plain and microfin tubes. The experimental apparatus was composed of three main parts; a refrigerant loop, a water loop and a water/glycol loop. The test section in the refrigerant loop was made of both a plain and a microfin copper tube of 9.52 mm outside diameter and 1.0 m length. The refrigerant was cooled by passing cold water through an annulus surrounding the test section. Tests were performed at a fixed refrigerant saturation temperature of $40^{\circ}C$ with mass fluxes of 100, 200, and 300 kg/$m^2s$. Test results showed that at similar mass flux the flow condensation HTCs of R134a were similar to those of R22 for both plain and microfin tubes. On the other hand, HTCs of R407C were lower than those of R22 by 11~l5% and 23~53% for plain and microfin tubes respectively. And HTCs of R410A were similar to those of R22 for a plain tube but lower than those of R22 by 10~21% for a microfin tube. In general, HTCs of a microfin tube were 2.0~3.0 times higher than those of a plain tube.