• Title/Summary/Keyword: R-estimation

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Validation of analytical method and antioxidant properties of Eriobotrya japonica Lindl. Leaf extract according to extraction solvent (추출용매 조건에 따른 비파 잎 추출물의 항산화 활성 및 유효성분의 분석법 밸리데이션)

  • Kim, Hyun-Hee;Heo, Mi-Ra;Lee, Songmi;Yim, Soon-Ho
    • Korean Journal of Food Science and Technology
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    • v.51 no.4
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    • pp.301-308
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    • 2019
  • The antioxidant properties of Eriobotrya japonica leaf extract were investigated using DPPH and ABTS radical scavenging assay. The 80% ethanol extract of leaves ($IC_{50}$ values for DPPH and ABTS were 13.9 and $10.9{\mu}g/mL$, respectively) and young leaves ($IC_{50}$ values for DPPH and ABTS were 20.7 and $17.3{\mu}g/mL$, respectively) showed high radical scavenging activity. Additionally, the quantitative method for estimation of ellagic acid and chlorogenic acid from E. japonica leaves was optimized by HPLC/DAD. This method showed high linearity of the calibration curve with a coefficient of correlation ($R^2$) equal to 0.999. The LOD values for ellagic acid and chlorogenic acid were 2.35 and $0.73{\mu}g/mL$, respectively, whereas LOQ values were 7.13 and $2.22{\mu}g/mL$, respectively. Recovery of the two compounds was 99.7-108.0% with RSD values less than 5.31%. These results suggest that 80% ethanol extract of E. japonica leaves could serve as a potential source of natural antioxidant for us in various industrial applications.

Profiles of Bacillus spp. Isolated from the Rhizosphere of Suaeda glauca and Their Potential to Promote Plant Growth and Suppress Fungal Phytopathogens

  • Lu, Ping;Jiang, Ke;Hao, Ya-Qiao;Chu, Wan-Ying;Xu, Yu-Dong;Yang, Jia-Yao;Chen, Jia-Le;Zeng, Guo-Hong;Gu, Zhou-Hang;Zhao, Hong-Xin
    • Journal of Microbiology and Biotechnology
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    • v.31 no.9
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    • pp.1231-1240
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    • 2021
  • Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.

Assessment of pregnancy-associated glycoprotein profile in milk for early pregnancy diagnosis in goats

  • Singh, Shiva Pratap;Natesan, Ramachandran;Sharma, Nandini;Goel, Anil Kumar;Singh, Manoj Kumar;Kharche, Suresh Dinkar
    • Animal Bioscience
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    • v.34 no.1
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    • pp.26-35
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    • 2021
  • Objective: This study was conducted to assess the level of pregnancy-associated glycoprotein (PAG) in whole and skim milk samples, and its suitability for early pregnancy diagnosis in goats. Methods: A two-step sandwich enzyme-linked immunosorbent assay (ELISA) system for estimation of milk PAG was developed and validated, which employed caprine-PAG specific polyclonal antisera. Whole and skim milk samples (n = 210 each) from fifteen multiparous goats were collected on alternate days from d 10 to d 30, and thereafter weekly till d 51 post-mating. PAG levels in milk samples were estimated by ELISA and the pregnancies were confirmed at d40 post-mating by transrectal ultrasonography (TRUS). Results: The level of PAG in whole and skim milk samples of both pregnant and nonpregnant goats remained below the threshold values until d 24 after mating. Thereafter, PAG concentration in whole and skim milk increased steadily in pregnant goats, whereas it continued below the threshold in non-pregnant does. The PAG profiles in whole and skim milk of pregnant goats were almost similar and exhibited strong positive relationship (r = 0.891; p<0.001). Day 26 post-mating was identified as the first time-point for significantly (p<0.05) higher milk PAG concentration in pregnant goats than to non-pregnant goats. When compared to TRUS examination for pregnancy diagnosis, the accuracy and specificity of PAG ELISA using whole and skim milk samples were 94.5% and 95.4%; and 95.3% and 100%, respectively. The high values of area-under-curve (0.904 [whole milk] and 0.922 [skim milk]), demonstrate outstanding discrimination ability of the milk assays. Among the sampling dates chosen, d 37 post-mating was identified as the best suitable time point for collection of milk samples to detect pregnancy in goats. Conclusion: The PAG concentration in whole and skim milk of goats collected between days 26 and 51 post-breeding can be used for the accurate prediction of pregnancy and may be useful for assisting management decisions in goat flocks.

A Study on the Effect of Technology Readiness Level and Commercialization Activities on the Success of Technology Commercialization: Focusing on Public Technology (기술사업화 성공에 대한 기술성숙도 및 사업화 활동의 영향에 관한 연구: 공공기술을 중심으로)

  • Shin, Yoonmi;Bong, Kang Ho;Park, Jaemin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.6
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    • pp.197-206
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    • 2021
  • There is growing interest in the function and role of public research institutes as "entrepreneurial actors" that can contribute to industrial development by commercializing excellent research outputs. On the other hand, their performance in the commercialization phase is insufficient because of the insufficient technological technology readiness level or repeatability. This study conducted probit model analysis to examine the effect of the technology readiness level and commercialization activities on the success of technology commercialization. The results showed that the possibility of success in technology commercialization increased with increasing TRL at the time of acquisition. In addition, the difference between the TRL at the time of acquisition and the current TRL (TRL Gap) does not affect technology commercialization on its own. It generates additional effects in conjunction with the TRL at the time of acquisition. Finally, the results show that technology commercialization is most likely to succeed if technology with a TRL 4-6 level is improved to TRL 9 level through a marginal effect estimation.

Crown Fuel Characteristics and Fuel Load Estimation of Pinus densiflora S. et Z. in Bonghwa, Gyeongbuk (경북 봉화 지역 소나무림에 대한 수관연료 특성과 연료량 추정)

  • Jang, Mina;Lee, Byungdoo;Seo, Yeonok;Kim, Sungyong;Lee, Young Jin
    • Journal of Korean Society of Forest Science
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    • v.100 no.3
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    • pp.402-407
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    • 2011
  • The objectives of this study were to analyze the crown vertical structure, crown bulk density, and to develop regression models for predicting crown fuel load using the data from 10 destructively sampled Pinus densiflora trees in Bonghwa, Gyeongbuk. The fuel loads were observed higher in the middle portion of the vertical distribution of crown followed by the lower portion and upper portion of Pinus densiflora, respectively. Approximately 25% crown fuel load was found in the needle while 33% was observed in the branches with <1 cm diameter with a total of 58% available fuel loads. The average crown bulk density was $0.45kg/m^3$, and $0.27kg/m^3$ of this was available in the needles and branches with <1 cm diameters. The resulting models in linear equations were able to account for 84% and 88% of the observed variation, while the allometric equations with diameter at breast height as the single predictor showed better results to account for 90% and 95% of the observed variation in the available crown fuel loads and total crown fuel loads, respectively. The suggested equations in this study could provide quantitative fuel load attributes for crown fire behavior models and fire management of red pine stands in Bonghwa areas.

Evaluation of Runoff Prediction from a Coniferous Forest Watersheds and Runoff Estimation under Various Cover Degree Scenarios using GeoWEPP Watershed Model (GeoWEPP을 이용한 침엽수림 지역 유출특성 예측 및 다양한 식생 피도에 따른 유출량 평가)

  • Choi, Jaewan;Shin, Min Hwan;Cheon, Se Uk;Shin, Dongseok;Lee, Sung Jun;Moon, Sun Jung;Ryu, Ji Cheol;Lim, Kyoung Jae
    • Journal of Korean Society on Water Environment
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    • v.27 no.4
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    • pp.425-432
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    • 2011
  • To control non-point source pollution at a watershed scale, rainfall-runoff characteristics from forest watersheds should be investigated since the forest is the dominant land use in Korea. Long-term monitoring would be an ideal method. However, computer models have been utilized due to limitations in cost and labor in performing long-term monitoring at the watersheds. In this study, the Geo-spatial interface to the Water Erosion Prediction Project (GeoWEPP) model was evaluated for its runoff prediction from a coniferous forest dominant watersheds. The $R^2$ and the NSE for calibrated result comparisons were 0.77 and 0.63, validated result comparisons were 0.92, 0.89, respectively. These comparisons indicated that the GeoWEPP model can be used in evaluating rainfall-runoff characteristics. To estimate runoff changes from a coniferous forest watershed with various cover degree scenarios, ten cover degree scenarios (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%) were run using the calibrated GeoWEPP model. It was found that runoff increases with decrease in cover degree. Runoff volume was the highest ($206,218.66m^3$) at 10% cover degree, whereas the lowest ($134,074.58m^3$) at 100% cover degree due to changes in evapotranspiration under various cover degrees at the forest. As shown in this study, GeoWEPP model could be efficiently used to investigate runoff characteristics from the coniferous forest watershed and effects of various cover degree scenarios on runoff generation.

Development and application of the estimation method of flood damage in the ungauged basin using satellite data (위성자료를 활용한 미계측유역의 홍수피해액 추산기법 개발 및 적용)

  • Yeom, Woong-Sun;Park, Dong-Hyeok;Ahn, Jaehyun
    • Journal of Korea Water Resources Association
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    • v.53 no.12
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    • pp.1183-1192
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    • 2020
  • Economic analysis is a basic step in establishing disaster mitigation measures, but it is difficult to verify the results due to uncertainty. Therefore, the scope of investigation and analysis is wide. However, it is difficult to predict the amount of damage caused by flooding because the collection of relevant data is limited in the ungauged basin. In this study, distributed runoff analysis and flooding analysis were performed, and a method of estimating the amount of flood damage in the ungauged basin was proposed using collectible social and economic indicators and flood analysis results. For distributed runoff analysis and flooding analysis, GRM (Grid based Rainfall-runoff Model) and G2D (Grid based 2-Dimensional land surface flood model) developed by Korea Institute of Civil engineering and Building Technology were used. The method of substituting collectible social and economic indicators into the simple method and improvement method was used to estimate the amount of flood damage. As a result of the study, it was possible to estimate the amount of flood damage using satellite data and social and economic indicators in the ungauged basin.

Impact of Triplochiton scleroxylon K. Schum Exploitation on Fern Richness and Biomass Potential in the Semi-Deciduous Rain Forest of Cameroon

  • Cedric, Chimi Djomo;Nfornkah, Barnabas Neba;Louis-Paul-Roger, Kabelong Banoho;Kevine, Tsoupoh Kemnang Mikelle;Awazi, Nyong Princely;Forje, Gadinga Walter;Louis, Zapfack
    • Journal of Forest and Environmental Science
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    • v.38 no.3
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    • pp.184-194
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    • 2022
  • Triplochiton scleroxylon K. Schum is the plant species most affected by logging activities in the East Region of Cameroon due to its market value. This logging has impacted the ecological niche of the fern plant for which limited research has been done. The aim of this study is to contribute towards improving knowledge of fern richness and biomass on T. scleroxylon within the Central African sub-region. Fern data collection was done on 20 felled/harvested T. scleroxylon where, in addition to fern inventory, fern biomass was collected by the destructive method. The diameter and height of T. scleroxylon measured were used as explanatory variables in allometric equations for fern biomass estimation. Fern inventory was characterized using diversity index. Eight fern species were recorded on T. scleroxylon (≈5 species/T. scleroxylon). The minimum diameter where fern could be found is 59.4 cm. The average fern biomass found was 23.62 kg/T. scleroxylon. Pearson correlation coefficient showed a positive correlation (r>0.55) between fern biomass and T. scleroxylon diameter. For allometric equation, the logarithmic model improved better the adjustment than the non-logarithmic model. However, the quality of the adjustment is improved more when only the diameter is considered as an explanatory variable. Fern biomass is estimated to 90.08 kg/ha-1 with 76.02 kg/ha-1 being lost due to T. scleroxylon exploitation in the study area. This study is a contribution towards increasing knowledge of fern diversity specific to T. scleroxylon, and also fern biomass contribution to climate change mitigation and the potential carbon loss due to T. scleroxylon exploitation.

Estimation on End Vertical Bearing Capacity of Double Steel-Concrete Composite Pile Using Numerical Analysis (수치해석을 이용한 이중 강-콘크리트 합성말뚝 연직지지력 평가)

  • Jeongsoo, Kim;Jeongmin, Goo;Moonok, Kim;Chungryul, Jeong;Yunwook, Choo
    • Journal of the Korean GEO-environmental Society
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    • v.23 no.12
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    • pp.5-15
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    • 2022
  • Conventionally, because evaluation methods of the bearing capacity for double steel pipe-concrete composite pile design have not been established, the conventional vertical bearing capacity equations for steel hollow pile are used. However, there are severe differences between the predictions from these equations, and the most conservative one among vertical bearing capacity predictions are conventionally adopted as a design value. Consequently, the current prediction method for vertical bearing capacity of composite pile prediction composite pile causes design reliability and economical feasibility to be low. This paper investigated mechanical behaviors of a new composite pile, with a cross-section composed of double steel pipes filled with concrete (DSCT), vertical bearing capacities were analyzed for several DSCT pile conditions. Axisymmetric finite element models for DSCT pile and surrounding ground were created and they were used to analyze effects on behaviors of DSCT pile pile by embedding depth, stiffness of plugging material at pile tip, height of plugging material at pile tip, and rockbed material. Additionally, results from conventional design prediction equations for vertical bearing capacity at steel hollow pile tip were compared with that from numerical results, and the use of the conventional equations for steel hollow pile was examined to apply to that for DSCT pile.

Derivation of Suitable-Site Environmental Factors in Robinia pseudoacacia Stands Using Type I Quantification Theory (수량화이론 I방법에 의한 아까시나무 임분의 적지 환경인자 도출)

  • Kim, Sora;Song, Jungeun;Park, Chunhee;Min, Suhui;Hong, Sunghee;Lim, Jongsoo;Son, Yeongmo
    • Journal of Korean Society of Forest Science
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    • v.111 no.3
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    • pp.428-434
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    • 2022
  • This study was conducted to derive the site index of forest productivity of Robinia pseudoacacia (honey plant) to characterize suitable planting sites and to investigate the effect of the site environmental factors on the site index using the quantification theory I method. The data used in the analysis were growth factors (stand age, dominant height, etc.) of the 6th national forest resources survey and various site environmental factors of a forest soil map (1:5,000). The average site index value of the R. pseudoacacia stand in Korea was 14 (range, 8 to 18). The environmental factors affecting the site index were parent rock, climatic zone, soil texture, local topography, and altitude. The accuracy of the estimation model using quantification theory I was only 33%. However, the correlation between the site index and the site environmental factors was statistically significant at the 1% level. Results of quantification analysis between site index and site environmental factors revealed that metamorphic and igneous rocks received high grades as parent rocks, climate zones received higher grades than central temperate zone, clay loam and silt loam received high grades in soil texture, and hillside received a high grade in local topography. Analysis of the partial correlation between site topographical factors and forest productivity (site index) found that soil class and altitude were partially correlated to x by 0.4129 and 0.4023, respectively, indicating that these factors are the most influential variables.