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The Study on the Relationship between Changes of Rumen Microflora and Bloat in Jersey Cow (저지종 젖소의 반추위 내 미생물 균총 변화와 고창증 발병간의 상관관계 연구)

  • Kim, Sang Bum;Oh, Jong Seok;Jeong, Ha Yeon;Jung, Young Hun;Park, Beom Young;Ha, Seung Min;Im, Seok Ki;Lee, Sung Sill;Park, Ji Hoo;Park, Seong Min;Kim, Eun Tae
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.2
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    • pp.106-111
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    • 2018
  • This study was conducted to investigate the relationship between changes of rumen microflora and bloat in Jersey cow. Jersey cows (control age: 42 months, control weight: 558kg; treatment age: 29 months, treatment weight 507kg) were fed on the basis of dairy feeding management at dairy science division in National Institute of Animal Science. The change of microbial population in rumen was analyzed by using next generation sequencing (NGS) technologies due to metabolic disease. The diversity of Ruminococcus bromii, Bifidobacterium pseudolongum, Bifidobacterium merycicum and Butyrivibrio fibrisolvens known as major starch fermenting bacteria was increased more than 36-fold in bloated Jersey, while cellulolytic bacteria community such as Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens was increased more than 12-fold in non-bloated Jersey. The proportion of bacteroidetes and firmicutes was 33.4% and 39.6% in non-bloated Jersey's rumen, while bacteroidetes and firmicutes were 24.9% and 55.1% in bloated Jersey's. In conclusion, the change of rumen microbial community, in particular the increase in starch fermenting bacteria, might have an effect to occur the bloat in Jersey cow.

Genetic Diversity in the Major Surface Protein Gene of Theileria Buffeli in Korean Indigenous Cattle (국내 한우의 타일레리아 주요항원단백질 유전자의 다양성)

  • Yu, Do-Hyeon;Li, Ying-Hua;Chae, Joon-Seok;Park, Jin-Ho
    • Journal of Veterinary Clinics
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    • v.27 no.5
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    • pp.501-507
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    • 2010
  • The aim of the current study was to analyze the diversity of the major surface protein (Msp) gene in Theileria buffeli, which is known as the major antigenic protein recognized by the immune system of the host. In addition, we characterized the diversification of the Msp gene and its relationship to with the pathogenicity of Theileria. Complete blood counts (CBC) and Theileria 18S rRNA PCR sequence analysis were performed for 177 Korean indigenous cattle (KIC) in Jeju Island. A total of 28 KIC (16 anemic and 12 non-anemic KIC) were then randomly selected based on 18s rRNA PCR positive samples for sequence analysis of the Theileria Msp gene, which was performed twice for each specimen. The resulting 56 Msp gene sequences were classified into five antigenicity types (type I to V), according to the variable region (517-571 bp), which exhibited high similarity (${\geq}$ 98.9%) to several available GenBank sequences (Theileria spp. from China-EU584237; T. sergenti from China-DQ078264; Theileria spp. from Thailand-AB081329; Theileria spp. from Japan-AB218442; T. sergenti from Japan-AB016280). The 56 Msp sequences consisted of 22, 15, 9, 8, and 2 cases of type I to type V Msp genes, respectively. The most prevalent type in both anemic and non-anemic KIC was type I (37.5% in anemic and 41.7% in non-anemic). Among the remaining types, type II was the most prevalent (37.5%) in anemic KIC, while type IV was the most prevalent (25%) in non-anemic KIC. The results of our study help confirm the diversity of Msp gene types and demonstrate that the gene type distribution of Msp genes varies among Theileria-infected KIC in Jeju Island.

The Perceived Occupational and Clients' Natures by the Participants of the Long-term Care-services Providers' Education in Korea (장기요양보호사 양성프로그램 참가자들이 인지한 직업특성 및 대상자 특성)

  • Auh, Seong-Yeon;Paik, Sun-Ah;Kim, Eun-Joo
    • Korean Journal of Health Education and Promotion
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    • v.27 no.5
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    • pp.51-61
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    • 2010
  • Objectives: The purpose of this study is to investigate the perception of LTCP trainees' on their professional. To accomplish the purpose, the perceived occupational nature and perceived clients' nature among the participants in the LTCP education are examined with the predicting variables. Methods: The participants of this study were composed of 320 people who have completed the long-term care provider certificate program. The participants were from 5 different long-term care provider licensing institution in the region of Gangdong-Gu, Seoul. Total of 320 self-administered questionnaires were distributed from June 2, 2008 to October 2, 2008 and 297 questionnaires were collected. Among them 283 surveys were analyzed. Results: In this model, active family support and recognition of task characteristics for the LTCP were the significantly predicting with beta of .187 and .507. The effect from active family support was remained significantly as model progressed. The magnitude of F-value and R-square value were significant. Conclusion: The results from this study will be helpful for the scholars who have investigated on the LTCP education program development as well as policy makers who have tried to supply dependable LTCP to the needy elderly and their families. In turn, the results from this study may empower constituents to make informed decisions about, and easily access, existing health and long-term care options.

Biological Activities of Essential Oil from Chamaecyparis obtusa (편백(Chamaecyparis obtusa) 정유의 항균, 항염, 항산화 효과)

  • Ahn Jeung-Youb;Lee Sung-suk;Kang Ha-young
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.4 s.48
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    • pp.503-507
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    • 2004
  • The essential oil from Chamaecyparis obtusa was investigated for biological activities in anti-oxidative, anti-inflammation and antibacterial method, respectively. The Growth inhibitory effect of C. obtusa oil on the bacteria was evaluated with MIC (minimum inhibitory concentration), $IC_{50}\;(50\%$ inhibitory concentration), and paper disc method. Two kinds of gram positive strains and two kinds of gram negative strains were used in this study. Gram positive strains were B. subtilis and S. aureus. and Gram negative strains were E. coli and P. aeruginosa. Gram positive strains showed much more intensive effect than gram negative strains. Anti-oxidative effect was investigated with DPPH (1,1-diphenyl-2-picrylhidrazyl) in methanol based and $IC_{50}\;was\;0.78\%.$ Our results suggest that the essential oil from Chamaecyparis obtusa has effects on anti-bacterial, anti- oxidative and anti-inflammation in in vitro and in uiuo. Then this material could be expect synergic effect with other candidated extracts and oils.

Experimental and analytical study of squat walls with alternative detailing

  • Leonardo M. Massone;Cristhofer N. Letelier;Cristobal F. Soto;Felipe A. Yanez;Fabian R. Rojas
    • Computers and Concrete
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    • v.33 no.5
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    • pp.497-507
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    • 2024
  • In squat reinforced concrete walls, the displacement capacity for lateral deformation is low and the ability to resist the axial load can quickly be lost, generating collapse. This work consists of testing two squat reinforced concrete walls. One of the specimens is built with conventional detailing of reinforced concrete walls, while the second specimen is built applying an alternative design, including stirrups along the diagonal of the wall to improve its ductility. This solution differs from the detailing of beams or coupling elements that suggest building elements equivalent to columns located diagonally in the element. The dimensions of both specimens correspond to a wall with a low aspect ratio (1:1), where the height and length of the specimen are 1.4 m, with a thickness of 120 mm. The alternative wall included stirrups placed diagonally covering approximately 25% of the diagonal strut of the wall with alternative detailing. The walls were tested under a constant axial load of 0.1f'cAg and a cyclic lateral displacement was applied in the upper part of the wall. The results indicate that the lateral strength is almost identical between both specimens. On the other hand, the lateral displacement capacity increased by 25% with the alternative detailing, but it was also able to maintain the 3 complete hysteretic cycles up to a drift of 2.5%, reaching longitudinal reinforcement fracture, while the base specimen only reached the first cycle of 2% with rapid degradation due to failure of the diagonal compression strut. The alternative design also allows 46% more energy dissipation than the conventional design. A model was used to capture the global response, correctly representing the observed behavior. A parametric study with the model, varying the reinforcement amount and aspect ratio, was performed, indicating that the effectiveness of the alternative detailing can double de drift capacity for the case with a low aspect ratio (1.1) and a large longitudinal steel amount (1% in the web, 5% in the boundary), which decreases with lower amounts of longitudinal reinforcement and with the increment of aspect ratio, indicating that the alternative detailing approach is reasonable for walls with an aspect ratio up to 2, especially if the amount of longitudinal reinforcement is high.

Chest wall injury fracture patterns are associated with different mechanisms of injury: a retrospective review study in the United States

  • Jennifer M. Brewer;Owen P. Karsmarski;Jeremy Fridling;T. Russell Hill;Chasen J. Greig;Sarah E. Posillico;Carol McGuiness;Erin McLaughlin;Stephanie C. Montgomery;Manuel Moutinho;Ronald Gross;Evert A. Eriksson;Andrew R. Doben
    • Journal of Trauma and Injury
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    • v.37 no.1
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    • pp.48-59
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    • 2024
  • Purpose: Research on rib fracture management has exponentially increased. Predicting fracture patterns based on the mechanism of injury (MOI) and other possible correlations may improve resource allocation and injury prevention strategies. The Chest Injury International Database (CIID) is the largest prospective repository of the operative and nonoperative management of patients with severe chest wall trauma. The purpose of this study was to determine whether the MOI is associated with the resulting rib fracture patterns. We hypothesized that specific MOIs would be associated with distinct rib fracture patterns. Methods: The CIID was queried to analyze fracture patterns based on the MOI. Patients were stratified by MOI: falls, motor vehicle collisions (MVCs), motorcycle collisions (MCCs), automobile-pedestrian collisions, and bicycle collisions. Fracture locations, associated injuries, and patient-specific variables were recorded. Heat maps were created to display the fracture incidence by rib location. Results: The study cohort consisted of 1,121 patients with a median RibScore of 2 (range, 0-3) and 9,353 fractures. The average age was 57±20 years, and 64% of patients were male. By MOI, the number of patients and fractures were as follows: falls (474 patients, 3,360 fractures), MVCs (353 patients, 3,268 fractures), MCCs (165 patients, 1,505 fractures), automobile-pedestrian collisions (70 patients, 713 fractures), and bicycle collisions (59 patients, 507 fractures). The most commonly injured rib was the sixth rib, and the most common fracture location was lateral. Statistically significant differences in the location and patterns of fractures were identified comparing each MOI, except for MCCs versus bicycle collisions. Conclusions: Different mechanisms of injury result in distinct rib fracture patterns. These different patterns should be considered in the workup and management of patients with thoracic injuries. Given these significant differences, future studies should account for both fracture location and the MOI to better define what populations benefit from surgical versus nonoperative management.

Clinical and Epidemiological Study of 1,165 Hospitalized Cases of Rotaviral Gastroenteritis Before and After the Introduction of Rotavirus Vaccine, 2006-2013 (로타바이러스 백신 도입 전후 입원한 로타바이러스 위장관염 1,165례의 역학 및 임상적 연구, 2006-2013년)

  • Sohn, Tae-Young;Lee, Chan-Jae;Kim, Yoon-Joo;Kang, Min-Jae;Kim, Sung-Hye;Lee, So-Yeon;Lee, Dae-Hyoung;Lee, Hae-Ran;Kim, Kwang-Nam
    • Pediatric Infection and Vaccine
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    • v.21 no.3
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    • pp.174-180
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    • 2014
  • Purpose: This study was performed to assess the clinical and epidemiological changes after the introduction of the rotavirus vaccine in Korea, as well as to determine the efficacy of the rotavirus vaccine among hospitalized rotaviral gastroenteritis patients over the past two years. Methods: We analyzed yearly and seasonal patterns of 1,165 inpatients who were hospitalized for rotaviral gastroenteritis under the age of 5 years between 2006 and 2013. We also conducted a survey among 460 gastroenteritis patients who were hospitalized between 2012 and 2013 regarding the rotavirus vaccination and the symptoms of gastroenteritis. Among those individuals surveyed, clinical indices were analyzed for 124 patients who were tested positive for the rotavirus antigen. Results: The incidence of Rotaviral gastroenteritis have decreased significantly by year 2010. After the introduction and widespread dissemination of the rotavirus vaccine, the onset of the disease and the seasonal peak have been delayed. Overall, the vaccinated group showed a lower rate of positivity than the unvaccinated group. Among the hospitalized rotaviral gastroenteritis patients, the vaccinated group had a shorter hospitalization period, less severe clinical symptoms of gastroenteritis, and better laboratory test results. Conclusions: After introduction of the rotavirus vaccine in Korea, there were two main trends observed: 1) the overall level of disease incidence was reduced; 2) the severity of rotaviral gastroenteritis cases also decreased. Based on this data, more children should receive vaccination in order to prevent the rotavirus infection and decrease the severity of rotaviral gastroenteritis.