• Parasites, Hosts and Diseases
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• 제38권2호
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• pp.85-90
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• 2000

To assess the relationship between the changes of cellular components and the production of Th 1 cytokine in the immune tissue, inbred C57BL/6 mice were orally infected with 40 cysts of 76K strain of Toxoplosma gondii. The sequential change of cell differentials and $IFN-{\gamma}$ production of splenocytes were analyzed by Diff-Quik stain and RT-PCR. There were no significant proportional changes of cellular components of splenocytes until day 4 postinfection (Pl) as compared to those of day 0, and the relative percentage of macrophages and neutrophils/eosinophils increased significantly (p<0.01) thereafter. The expression of $IFN-{\gamma}$ mRNA of $CD3^{-}$ cells was observed from day 1 Pl at a low level. However, $IFN-{\gamma}$ production of $CD3^{+}$ cells increased significantly from day 4 Pl (p<0.01) which progressively increased thereafter. These findings provide the relative percentages of granulocytes and macrophages were increased in conjunction with increase of total number of splenocytes after oral infection with T. gondii in the susceptible murine hosts, and lymphocytes were the major cellular components and the important source of $IFN-{\gamma}$.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.463-466
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• 2009

The numerical simulation optimized by Four Dimensional Data Assimilation (FDDA) with Quick Scatterometer (QuikSCAT) data is carried out to evaluate wind resource characteristics at various heights in the southeastern area of the Korean Peninsula, where wind farms are planned to be built on on- and off-shore as well as comparable diurnal wind variations are characterized at the surface. The temporal and spatial distributions of modeled wind speeds showed good agreement with the observations based on the temporal variation analysis. Model results indicate that the higher model is performed in resolution, the more precise results is at turbine hub height. Occasionally, wind speed variations for each numerical resolution has a different regional and seasonal variations. In the coast area, hub height wind speed of 9km-resolution is simillar to that of 3km-resolution. On the other hand, hub height wind speed of 3km-resolution is simillar to that of 1km-resolution in the Jiri mountainous area.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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• pp.989-992
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• 2006

The new approach to the problem of oil spill detection consisting in combined use of all available quasiconcurrent satellite information (AVHRR NOAA, TOPEX/Poseidon, Jason-1, MODIS Terra/Aqua, QuikSCAT) is suggested. We present the results of the application of the proposed approach to the operational monitoring of seawater condition and pollution in the coastal zone of northeastern Black Sea conducted in 2006. This monitoring is based on daily receiving, processing and analysis of data different in nature (microwave radar images, optical and infrared data), resolution and surface coverage. These data allow us to retrieve information on seawater pollution, sea surface and air-sea boundary layer conditions, seawater temperature and suspended matter distributions, chlorophyll a concentration, mesoscale water dynamics, near-surface wind and surface wave fields. The focus is on coastal seawater circulation mechanisms and their impact on the evolution of pollutants.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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• pp.1027-1030
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• 2006

Development of diurnal warming in the open Okhotsk Sea during the daytime and calm conditions was studied using sea surface temperature (SST) fields retrieved from NOAA AVHRR, Terra and Aqua MODIS, Aqua AMSR-E and ADEOS-II AMSR data. Sea surface wind fields were estimated from AMSR-E/AMSR measurements as well as were obtained from QuikSCAT scatterometer. Weak winds and cloudless conditions were observed in the central area of anticyclone, which moved slowly on 28-30 June 2003 east off Sakhalin. The area where the amplitude of the diurnal SST signal ${\Delta}T$ was significant also shifted slowly and had or circular or elongated shape. The ${\Delta}T$ was estimated relative to the SST values in the areas surrounding the centre of anticyclone where wind speed W exceeded 5- 6 m/s. The diurnal variations of SST, day-night differences were computed using NOAA-12 and NOAA-16 AVHRRderived data. Analysis of simultaneous SST and W fields showed that the increase of W from 0 to 5-6 m/s causes the decrease of ${\Delta}T$ to zero. Maximum warming exceeded $8^{\circ}C$ and was observed in the centre of anticyclone where W = 0 m/s. So strong heating was likely due to the increased chlorophyll a concentration in the area under study that follows from analysis of satellite ocean colour data.

• 한국임상수의학회지
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• 제25권4호
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• pp.274-279
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• 2008

This study was performed to investigate the best staining method for the somatic cell classification of dairy goat milk. Dairy goat milk samples, which were collected randomly from a dairy goat farm in Jeollanam-do, South Korea, were stained and analyzed with direct microscopic method, using 5 different staining methods; Wright's stain, Giemsa stain, Diff-quik stain, Newman's stain and Pyronin Y-Methyl Green stain, respectively. Among them, The Newman's staining was found to be the most rapid and effective method, for it required the shortest time for staining and provided the easiest way to classify somatic cells.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 추계학술대회 논문집
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• pp.274-277
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• 2008

To evaluate high-resolution wind resources for local and coastal area with complex terrain was attemped to combine the prognostic MM5 mesoscale model with CALMET diagnostic modeling this study. Firstly, MM5 was simulated for 1km resolution, nested fine domain, with FDDA using QuikSCAT seawinds data was employed to improve initial meteorological fields. Wind field and other meteorological variables from MM5 with all vertical levels used as initial guess field for CALMET. And 5 surface and 1 radio sonde observation data is performed objective analysis whole domain cells. Initial and boundary condition are given by 3 hourly RDAPS data of KMA in prognostic MM5 simulation. Geophysical data was used high-resolution terrain elevation and land cover(30 seconds) data from USGS with MM5 simulation. On the other hand SRTM 90m resolution and EGIS 30m landuse was adopted for CALMET diagnostic simulation. The simulation was performed on whole year for 2007. Vertical wind field a hour from CALMET and latest results of MM5 simulation was comparison with wind profiler(KEOP-2007 campaign) data at HAENAM site.

• 대한세포병리학회지
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• 제5권1호
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• pp.1-9
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• 1994

The cytological and ultrastructural findings of Pneumocystis carinii(PC) obtained from rats by bronchoalveolar lavage (BAL) are described. All developmental forms of the PC organisms were obtained in the lavage fluid. Papanicolaou stain revealed conglomeration of PC as a foamy cast. The cystic walls of PC were well identified on Gomori's methenamine silver stain. Trophozoites and intracystic bodies were stained by Giemsa and Diff-Quik techniques. Some PC organisms were seen within the alveolar macrophages. Ultrastructurally, the cysts were almost circular in shape, and were nearly devoid of surface tubular extensions. The wall of the cyst was composed of an unit membrane, an intermediate electron lucent layer and an external electron dense layer The cysts frequently contained intracystic bodies, so called sporozoites. Occasionally empty or collapsed cysts with no intracystic bodies, and precysts were found. Trophozoites were variable in size and shape with abundant tubular extensions along the single electron dense pellicle. BAL is a useful method for concentrating the various morphologic forms of PC organisms, and is a rapid diagnostic method for PC pneumonia.

• 대한가정학회지
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• 제49권2호
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• pp.37-49
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• 2011

The present study investigated the semantic and syntactic processes in Korean children's sentence comprehension by measuring event-related brain potentials(ERPs). The subjects were 18 right-handed, healthy native Korean children(1st graders), who were free from any hearing problems. While the children listened to the auditory sentence stimuli, ERPs were recorded with the electrodes mounted in a 'Quik-Cap' on the subject's head. The ERP data were recorded and analyzed using the NeuroScan 4.3 and EEGLABv6.03b programs. For statistical analyses, descriptive statistics, three-way repeated measures ANOVA, and t-tests were performed using a SPSS 15.0 PC program. The results indicated that semantic violations elicited a negativity (N400) ranging from 300 to 500 msec. For syntactic violations, children displayed a positivity (P600) ranging from 900 to 1,100 msec. The discovery of N400 and P600 in semantic and syntactic processes respectively, confirmed the suggestion that Indo-European and Korean languages share a common mechanism for sentence comprehension.

• 대한수의학회지
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• 제61권3호
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• pp.25.1-25.7
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• 2021

The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential has been attributed to its life-long neoteny, characterized by the exhibition of embryonic characteristics at the adult stage. A recent study provided a detailed morphological analysis of the sperm morphology of the Ambystoma mexicanum using routine and detailed histological techniques. The primary purpose of the present study is to describe a simple and inexpensive method for evaluating the morphology of axolotl sperm. In this study, spermatophore structures were collected and spread on slides and air-dried. The slides were stained with periodic acid Schiff, toluidine blue, Masson's trichrome, Giemsa, Spermac, and Diff-Quik dye for a morphological examination. The slides were coated with gold/palladium for a scanning electron microscopy examination. The sperm of the axolotl consisted of an elongated head, a neck, and a flagellum covered with an undulating membrane. The lengths of the midpiece, tail, and head were 8.575 ㎛, 356.544 ㎛, and 103.661 ㎛, respectively. In the flagellum part, the wavy membrane structure, whose function has not been explained, surrounds the tail. The data obtained from this study will constitute an important step in designing future research on the reproductive and regeneration capacity of the axolotl.

• 한국환경과학회지
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• 제14권11호
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• pp.1015-1026
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• 2005

The southeastern coastal area of the Korean peninsula has a complex terrain including an irregular coastline and moderately high mountains. This implies that mesoscale circulations such as mountain-valley breeze and land-sea breeze can play an important role in wind field and ocean forcing. In this study, to improve the accuracy of complex coastal rind field(surface wind and sea surface wind), we carried out the sensitivity experiments based on PBL schemes in PSU/NCAR Mesoscale Model (MM5), which is being used in the operational system at Korea Meteorological Administration. Four widely used PBL parameterization schemes in sensitivity experiments were chosen: Medium-Range Forecast (MRF), High-resolution Blackadar, Eta, and Gayno-Seaman scheme. Thereafter, case(2004. 8. 26 - 8. 27) of weak-gradient flows was simulated, and the time series and the vertical profiles of the simulated wind speed and wind direction were compared with those of hourly surface observations (AWS, BUOY) and QuikSCAT data. In the simulated results, the strength of rind speed of all schemes was overestimated in complex coastal regions, while that of about four different schemes was underestimated in islands and over the sea. Sea surface wind using the Eta scheme showed the highest wind speed over the sea and its distribution was similar to the observational data. Horizontal distribution of the simulated wind direction was very similar to that of real observational data in case of all schemes. Simulated and observed vertical distribution of wind field was also similar under boundary layer(about 1 km), however the simulated wind speed was underestimated in upper layer.