• 한국연초학회지
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• 제12권1호
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• pp.29-38
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• 1990

Until relatively recently plant scientists have made little use of immunological techniques. Now, however, more and more researchers are discovering the powder of these techniques for the screening of immunomodulators and for the detection, quantitative determination and localization of compounds in plant materials. Especially, the recent developments in the fields of plant biotechnology and plant genetic engineering make it even more important for forkers in the plant sciences to become acquainted with the more sophisticated methods. The possible methods include onestep purification of antigens, visualization in situ by immunocytochemis try and on polyacrylam ids gel s by ni trocellulose Western blotting and quantification by various immunoassay. Among them, in this reviews, the quantitative determination methods are to be reviewed. There are several kinds of methods for the quantitative determination of plant constituents such as colorimetry, TLC, GLC, DCC, UV derivatization, densitometry and HPLC. When the complexity of plant constituents is considered. densitometry and HPLC have many advantages in sensitivity and separation ability. After a 11 some advarltages of two methods meritiorled above, all of these methods have many disadvantages and inconveniences. Previous purification for the application of all these methods make them less sensitive and more tedious. Immunoassay can solve these problems in part. But immunoassay also has some limitations. Specificity of immunoassay, contrary, can be considered to be disadvantages. Including this the advantages and disadvantages of immunoassay are to be discussed.

• Educational Technology International
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• 제23권2호
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• pp.157-181
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• 2022

Scale scores for cognitive domains have been used as an important indicator for both academic achievement and clinical diagnosis. For example, in education, Cognitive Abilities Test (CogAT) has been used to measure student's capability in academic learning. In a clinical setting, Cognitive Impairment Screening Test utilizes items measuring cognitive ability as a dementia screening test. We demonstrated a procedure of generating cognitive ability test items similar as in CogAT but the theory associated with the generation is totally different. When creating cognitive test items, we applied automatic item generation (AIG) that reduces errors in predictions of cognitive ability but attains higher reliability. We selected two cognitive ability test items, categorized as a time estimation item for measuring quantitative reasoning and a paper-folding item for measuring visualization. As CogAT has widely used as a cognitive measurement test, developing an AIG-based cognitive test items will greatly contribute to education field. Since CLASS is the only LMS including AIG technology, we used it for the AIG software to construct item models. The purpose of this study is to demonstrate the item generation process using AIG implemented within CLASS, along with proving quantitative and qualitative strengths of AIG. In result, we confirmed that more than 10,000 items could be made by a single item model in the quantitative aspect and the validity of items could be assured by the procedure based on ECD and AE in the qualitative aspect. This reliable item generation process based on item models would be the key of developing accurate cognitive measurement tests.

• 항공우주시스템공학회지
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• 제12권4호
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• pp.57-63
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• 2018

적외선 온도 측정 기법은 비접촉식 방법으로 모형의 표면 온도를 가시화할 수 있는 기법이다. 그러나 획득할 수 있는 결과는 2차원 온도 결과로 정량적인 결과를 획득하기에는 한계가 있다. 본 연구는 3차원 매핑 기법을 적외선 온도 측정 기법에 적용하는 것이 목표이다. 풍동 실험은 국방과학연구소에서 보유하고 있는 중형 아음속 풍동에서 수행했으며, 대상 모형은 오자이브 실린더이다. 시험 조건은 유속 20 m/s에서 80 m/s, 받음각은 $0^{\circ}{\sim}90^{\circ}$이다. 3차원 매핑 기법은 마커를 이용하여 실제 모형의 위치정보와 적외선 이미지 상의 위치 정보를 대응시키는 방법을 사용하였다. 그 결과, 모형의 박리점이 이론적인 값과 매우 일치하는 것을 확인하였다.

• 한국가시화정보학회지
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• 제14권3호
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• pp.28-31
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• 2016

In this study, we investigate the wake characteristics in two cases which are laminar inflow and turbulent inflow. To solve the flow with wind turbines and its wake, we use large eddy simulation (LES) technique with actuator line method (ALM) and turbulent inflow of Turbsim. Turbulent inflow which contains the characteristic of the stable atmospheric boundary layer is used. We perform the quantitative analysis of velocity deficit and turbulence intensity in two cases. Time series of velocity deficit at the first, the second column in two cases are compared to observe the performance of wind turbine. The performance in the first column in laminar inflow is overestimated compared to that in turbulent inflow. And we observe that wake in the case with turbulent inflow drive to the span-wise direction and wake recovery in turbulent inflow is more effective. In quadrant analysis of Reynolds stress, the ejection and the sweep motion in turbulent inflow case are bigger than those in laminar inflow case.

• Journal of Korean Neurosurgical Society
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• 제56권5호
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• pp.400-404
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• 2014

Objective : Clip artifacts limit the visualization of intracranial structures in CT scans from patients after aneurysmal clipping with cobalt alloy clips. This study is to analyze the parameters influencing the degree of clip artifacts. Methods : Postoperative CT scans of 60 patients with straight cobalt alloy-clipped aneurysms were analyzed for the maximal diameter of white artifacts and the angle and number of streak artifacts in axial images, and the maximal diameter of artifacts in three-dimensional (3-D) volume-rendered images. The correlation coefficient (CC) was determined between each clip artifact type and the clip blade length and clip orientation to the CT scan (angle a, lateral clip inclination in axial images; angle b, clip gradient to scan plane in lateral scout images). Results : Angle b correlated negatively with white artifacts (r=-0.589, p<0.001) and positively with the angle (r=0.636, p<0.001) and number (r=0.505, p<0.001) of streak artifacts. Artifacts in 3-D images correlated with clip blade length (r=0.454, p=0.004). Multiple linear regression analysis revealed that angle b was the major parameter influencing white artifacts and the angle and number of streak artifacts in axial images (p<0.001), whereas clip blade length was a major factor in 3-D images (p=0.034). Conclusion : Use of a clip orientation perpendicular to the scan gantry angle decreased the amount of white artifacts and allowed better visualization of the clip site.

• 한국분무공학회지
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• 제18권2호
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• pp.87-93
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• 2013

In this paper, high-pressure injection characteristic of servo hydraulic injector as the key component of diesel CRDi system, which is driven by solenoid and piezo-actuator were examined by experimental analysis. High-pressure injection characteristic of standard diesel fuel injected at high pressure up to 160 MPa was investigated at high-pressure chamber by using a high-speed camera for spray visualization and quantitative analysis. By this study, we found that the piezo-driven injector has better performances in controlling the fuel injection with the high pressure, including fuel quantity, spray penetration length and spray velocity, than that of a solenoid-driven injector. In particular, the needle response time for start of injection in piezo-driven injector was faster of about $125{\mu}s$ than that of solenoid-driven injector. Consequently, it is known that the piezo-driven injector has more degrees of freedom in controlling the fuel injection with the high pressure than solenoid-driven injector.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2003년도 추계학술대회 논문집
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• pp.81-82
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• 2003

We have developed the method for the conjugation of biotinylated DNA to streptavidin-coated QDs. QD-DNA conjugates and a high-sensitive fluorescence imaging technique are adopted to visualize gene transport across the membrane of the live cell in real time. Endocytotic cellular uptake of oligonucleotide and electrically-mediated plasmid DNA transfer into the live cell are monitored by a quantitative microscopic imaging system. Long-term kinetic study enables us to reveal the unknown mechanisms and rate-limiting steps of extracellular and intracellular transport of biomolecules. We designed experimental protocols to conjugate the oligonucleotide or the plasmid DNA to commercially available streptavidin-coated QDs. Gel electrophoresis is used to verify the effect of incubation time and the molar ratio of QDs and DNA on the conjugation efficiency. It is possible to fractionate the QD-DNA conjugates according to the DNA concentration and obtain the purified conjugates by a gel extraction technique.

• 수산해양기술연구
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• 제50권1호
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• pp.39-49
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• 2014

Morphological and positional characteristics of anchovy aggregations, confirmed by trawling, were examined in two locations of the southern part of theWest Sea (T1) and the western side of South Sea (T11) of South Korea. Morphological characteristics (mean length, height and area) of the anchovy aggregations at T1 were smaller than those at T11, however the positional characteristics (distributional depth and bottom depth) of the aggregations at T1 were larger than those at T11. Diverse dataset such as the ship's cruise track, the cruse map, and interpolated three-dimensional-like water temperature were visualized in multiple dimensions. For a comprehensive understanding of the anchovy aggregations within their surrounding circumstances, the interpolated water temperature transferred to the location of anchovy aggregations at both stations were visualized based on geospatial information. Using quantitative investigation, the overall range of change in water temperature and salinity of anchovy aggregations at stations was considerably small. However, the water temperature and salinity of anchovy aggregations at T11 were somewhat higher than those at T1.

• 한국가시화정보학회지
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• 제13권1호
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• pp.49-53
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• 2015

Recently, researches for droplet impact phenomena have been faced a new phase in the direction of studying the effect of complex external conditions (e.g. wettability, temperature, morphology, electric field, etc.) for depth understanding and precise controlling in various applications. Hence, here we investigated the electrified droplet impact phenomena, because there were few quantitative researches for electrified droplet impact when we considering many real applications such as electrospray, electrohydrodynamic (EHD) jet printing. To observe interaction effect of surface charge between substrate and droplet simultaneously, micro-droplets with various Reynolds number (Re) and Weber number (We) were dripped on super-hydrophobic surface with existence and nonexistence of electrical surface charge. It shows three kinds of impact behaviors, fully bouncing, partial bouncing, and splashing with different We. Also, charged droplet bounced higher on electrically charged surface than on non-charged surface. Additionally, transition regions of three impact behaviors were classified quantitatively with water hammer pressure value, which means instant pressure inside droplet at the impact moment.

• 한국화재소방학회논문지
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• 제21권1호
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• pp.82-89
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• 2007

본 연구에서는 Jet fan 근접 거리에서 화재 발생 시 최소거리의 Jet fan을 이용하여 연기를 제어하기 위하여 PIV에 의한 가시화실험을 실시하였고, 또한 실험을 통해 얻어진 결과와 FDS 수치해석 그 결과를 비교, 분석 하였다. 터널 내 화재발생 시 Jet fan 설치에 따른 효율적인 연기제어 방식의 정량적 데이터 분석 결과 근접 Jet fan의 작동은 화원과 25m 이상의 거리에서만 가동해야 한다. 또한 Jet fan과 화원의 이격거리가 50m 부근에서 가장 안정적으로 연기가 제어되며 75m 이상의 거리에서는 터널 중앙 부근에서 과풍속 경향이 나타나며 제연되었다.