• Investigative Magnetic Resonance Imaging
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• 제26권2호
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• pp.135-149
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• 2022

Purpose: To assess clinically significant imaging findings of malignant intramammary lymph nodes (IMLNs) in breast cancer patients and to evaluate their diagnostic performance in predicting malignant IMLN. Materials and Methods: A total of 110 cases with IMLN of BI-RADS category 3 or more, not typical benign IMLN, in MR of breast cancer patients between January 2016 and January 2021 were retrospectively reviewed. After excluding 33 cases, 77 cases were finally included. Among them, 58 and 19 were confirmed as benign and malignant, respectively. Qualitative and quantitative MR imaging features of the IMLN were retrospectively analyzed. Sizes and final assessment categories of IMLN on MRI, mammography, and ultrasound were reviewed. Diagnostic performances of imaging features on MRI, mammography, and ultrasound were then evaluated. Results: For qualitative MR features, shape, margin, and preserved central hilum were significantly different between benign and malignant groups (P < 0.05). For quantitative MR features, long diameter over 6 mm, short diameter over 4 mm, and cortical thickening over 3 mm showed high sensitivities in predicting malignant IMLNs (89.5%, 94.7%, and 100%, respectively). Size exceeding 1 cm showed high specificity and accuracy in predicting malignant IMLN on MR, mammography, and ultrasound (91.4% and 80.5%; 96.6% and 79.25; 98.3% and 80.5%, respectively). Conclusion: Various MR imaging features and size can be helpful for predicting malignant IMLN in breast cancer patients.

• The Journal of Korean Physical Therapy
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• 제12권3호
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• pp.311-317
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• 2000

The puroose of the study was carried out to investigate the change of bacterial growth in vitro according to intensities and exposure time. to basic data far ultrasound and clinical research. The Staphylococcus aureus which are commonly isolated from open wound were incubated in an incubator for 24 hours following expoure 1MHz continuous ultrasound(CUS). Then quantitative bacterial counts were obtained. The results were as following. 1. The groups CUS was appied changed in bacterial growth according to intensities and time respectively. 2. The groups CUS was appied showed the inhibitory effect of bacterial growth. 3. The number of S. aureus significantly reduced to fellowing expoure $3.0w/cm^2$ CUS during 20min.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2777-2783
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• 2014

The purpose of this study was to explore the expression of ATAD2 in ovarian tumor tissue as well as its relationship with degree of malignancy. Tumor tissue from 110 cases of ovarian cancer was collected in accordance with the Declaration of Helsinki for evaluation of ATAD2 expression iimmunohistochemistry, quantitative PCR (qPCR) and Western blotting. The correlation between the ATAD2 expression and and the prognosis of ovarian cancer was evaluated by Cox regression model. In addition, HO-8910 and OVCAR-3 cells were transfected with two siRNAs targeting ATAD2. Cell viability was evaluated with MTT assay, and cell migration by transwell migration assay. ATAD2 was shown to be highly expressed in 65.5% (72/110) of ovarian cancer cases, both at transcriptional and protein levels. Moreover, highly expression was positively correlated with degree of malignancy. Knock-down of ATAD2 in HO-8910 and OVCAR-3 cells was found to reduce cell migration. In addition, follow-up visits of the patients demonstrated that the 5-year survival rate was lower in patients with high expression of ATAD2. Our study suggested that ovarian tumor tissue may have highly expressed ATAD2, which is associated with tumor stage, omentum-metastasis, ascites and CA-125. Increased ATAD2 may play important roles in tumor proliferation and migration. ATAD2 could serve in particular as a prognostic marker and a therapeutic target for ovarian cancer.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.307-310
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• 2021

지난 몇년간 유방 초음파영상을 이용한 신호 및 영상처리 기술과 자동 영상 최적화 기술, 유방 종괴 자동 검출 및 분류 기술 등, 컴퓨터 보조 진단(computer-aided diagnosis, CAD)을 활용하는 연구들이 활발히 진행되어지고 있다. 컴퓨터진단기술이 개발될수록 암의 조기 발견이 정확하고 빠르게 진행되어 건강 보험과 환자의 검사 빙용을 줄일 수 있고 조직 검사에 대한 불안감을 없앨 수 있을 것으로 기대된다. 본 논문에서는 GLCM(gray level co-occurrence matrix)을 사용하여 초음파 영상에서 종양의 정량적 분석을 진행하여 컴퓨터보조 진단에 활용 가능성을 실험하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1285-1290
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• 2014

The aim was to determine whether ultrasound targeted microbubble destruction (UTMD) promotes dual targeting of HSP72 and HSC70 for therapy of castration-resistant prostate cancer (CRPC), to improve the specific and efficient delivery of siRNA, to induce tumor cell specific apoptosis, and to find new therapeutic targets specific of CRPC.VCaP cells were transfected with siRNA oligonucleotides. HSP70, HSP90 and cleaved caspase-3 expression were determined by real-time quantitative polymerase chain reaction and Western blotting. Apoptosis and transfection efficiency were assessed by flow cytometry. Cell viability assays were used to evaluate safety. We found HSP72, HSC70 and HSP90 expression to be absent or weak in normal prostate epithelial cells (RWPE-1), but uniformly strong in prostate cancerous cells (VCaP). UTMD combined with dual targeting of HSP72 and HSC70 siRNA improve the efficiency of transfection, cell uptake of siRNA, downregulation of HSP70 and HSP90 expression in VCaP cells at the mRNA and protein level, and induction of extensive tumor-specific apoptosis. Cell counting kit-8 assays showed decreased cellular viability in the HSP72/HSC70-siRNA silenced group. These results suggest that the combination of UTMD with dual targeting HSP70 therapy for PCa may be most efficacious, providng a novel, reliable, non-invasive, safe targeted approach to improve the specific and efficient delivery of siRNA, and achieve maximal effects.

• 대한의생명과학회지
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• 제25권4호
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• pp.381-389
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• 2019

The physiological function test is the only patient contact area in the field of clinical laboratory. We need to recruit and encourage the experts due to requiring the expertise and long time for examination. However, there is currently no rule for estimating optimal workforce in the field of physiological function tests. The purpose of this study is to establish the basis of the studies for mid- to long-long term job creation in the field of ultrasound by evaluating the number of appropriate tests and appropriate workforce. We calculated the quantitative and qualitative workload for the number of appropriate tests and appropriate workforce using online questionnaire. All statistical analyses were performed using SPSS version 18.0 (SPSS Inc., Chicago, IL, USA). A total of 216 respondents were 48 (22.2%) male and 168 (77.8%) female. A total of 157 laboratories were 62 (39.5%) for echocardiography, 91 (58.0%) for the transcranial Doppler (TCD) and 4 (5.7%) for the carotid ultrasound. The mean number of appropriate tests was 10 ± 2 in the echocardiography, 9 ± 2 in TCD and 11 ± 2 in the carotid ultrasound. In addition, the number of laboratories required to recruit employees for appropriate workforce was 19 in echocardiography, 18 in TCD, and 0 in carotid ultrasound. The number of hospital required to recruit workforce were 7 primary hospitals, 22 secondary hospitals, 7 third hospitals. This study can be used as an important data as the first study at present time when the data on the workforce status and work environment of the ultrasonic laboratories is insufficient. Based on the quantitative and qualitative workloads, the number of appropriate tests and appropriate workforce can support mid- to long-long term job creation in the field of ultrasound.

• 한국음향학회지
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• 제31권4호
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• pp.244-252
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• 2012

본 연구의 목적은 골절 위험도가 높은 대퇴골에서 음속 및 광대역 초음파 감쇠와 같은 음향특성과 골밀도 사이의 상관관계를 조사하는 것이다. 이를 위해 0.5 및 1.0 MHz의 중심주파수를 갖는 두 쌍의 초음파 트랜스듀서와 함께 투과법을 이용하여 생체 외 조건에서 15개의 소 대퇴골 해면질골 샘플의 음속 및 광대역 초음파 감쇠를 측정하였다. 또한 마이크로 컴퓨터 단층촬영법을 이용하여 해면질골 샘플의 단위체적당 골밀도를 측정하였다. 골밀도는 0.5 및 1.0 MHz 초음파 트랜스듀서를 이용하여 측정된 음속 및 광대역 초음파 감쇠와 모두 강한 상관관계를 나타냈으며, 골밀도와 0.5 MHz 초음파 트랜스듀서를 이용하여 측정된 광대역 초음파 감쇠 사이에 가장 높은 상관관계가 존재하였다. 이와 같은 결과는 생체 외 조건의 대퇴골에서 측정된 음향특성이 대퇴골의 골밀도를 예측하기에 충분한 지표라는 것을 의미한다.

• Korean Journal of Radiology
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• 제22권11호
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• pp.1841-1849
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• 2021

Objective: To prospectively investigate the feasibility of shear wave elastography (SWE) as a new quantitative and objective method for evaluating the stiffness of the gastrocnemius medialis (GM) muscle during passive stretching in patients with Parkinson's disease (PD). Materials and Methods: SWE of the GM muscle was performed in 28 patients with PD [13 female and 15 male; mean age ± standard deviation (SD): 63.0 ± 8.5 years] and 12 healthy controls (5 female and 7 male; mean age ± SD: 59.3 ± 6.4 years) during passive ankle rotation. A Young's modulus-ankle angle curve was constructed. The GM slack angle and baseline Young's modulus (E₀) were compared between the markedly symptomatic and mildly symptomatic sides of patients with PD, and healthy controls. Additionally, the correlation between the GM slack angle and the severity of rigidity, and the observer reproducibility of SWE in determining the GM slack angle were evaluated. Results: The GM slack angle was smaller on both the markedly and mildly symptomatic sides in patients with PD than in healthy controls (mean ± SD of -29.13° ± 3.79° and -25.65° ± 3.39°, respectively, vs. -21.22° ± 3.52°; p < 0.001 and p = 0.006, respectively). Additionally, in patients with PD, the GM slack angle on the markedly symptomatic side was smaller than that on the mildly symptomatic side (p = 0.003). The E0 value was lower on both the markedly and mildly symptomatic sides in patients with PD than in healthy controls (mean ± SD of 10.11 ± 2.85 kPa and 10.08 ± 1.88 kPa, respectively, vs. 12.23 ± 1.02 kPa; p = 0.012 and p < 0.001, respectively). However, no significant difference was found between the markedly and mildly symptomatic sides in patients with PD (p = 0.634). A negative linear relationship was observed between the GM slack angle and lower limb rigidity score on the markedly symptomatic side in patients with PD (r = -0.719; p < 0.001). The intraclass correlation coefficients for observer reproducibility of SWE ranged from 0.880 to 0.951. Conclusion: The slack angle determined by SWE may be a useful quantitative and reproducible method for evaluating muscle stiffness in patients with PD.

• 전자공학회논문지SC
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• 제47권5호
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• pp.43-51
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• 2010

의료 초음파 신호의 인체내 감쇠지수는 검사대상 조직의 병리학적 특성을 반영할 뿐 아니라 다른 여러 의료 초음파 지수들의 정확한 예측을 위해 선행하여 측정해야 하는 중요한 정량적 정보 중 하나이다. 그러나 초음파 감쇠지수의 주파수 선택적 감쇠특성을 이용한 주파수 영역에서의 정량적 감쇠지수 예측 방법은 계산량이 많아 실시간 적용에 많은 어려움이 있고, 상대적으로 계산량이 적은 시간 영역의 감쇠지수 예측 방법은 전송 펄스의 회절효과를 잘 보상하지 못하는 단점이 있다. 표준 반향신호를 이용하여 전송 펄스의 회절효과를 보상하는 시간 영역의 예측 알고리듬인 VSA(Video Signal Analysis) 방법은 광대역 펄스를 이용하는 경우, 원거리에서 반향된 신호의 왜곡이 발생하여 예측 정확도가 저하되는 단점이 있다. 본 논문에서는 그 단점을 해결하기 위해 적응 대역필터를 이용한 초음파 감쇠지수 예측 알고리듬을 제안한다. 제안된 방식은 반향 경로를 따라 누적된 신호 감쇠를 고려하여 적응 대역필터의 중심 주파수를 이동시킴으로써, 기존의 고정 대역필터를 사용하는 방법보다 예측 정확도와 정밀도를 높인다. 인체 조직의 초음파 반향특성을 모방한 컴퓨터 모의실험과 실제 TM(tissue-mimicking) phantom을 이용한 실험에서, 광대역 전송 펄스를 사용하는 경우보다 반향 깊이에 따른 상대적 echogenicity의 왜곡이 크게 감소하여 평균적으로 예측 감쇠지수의 정확도가 5.1% 향상되었고, 예측 편차도 기존의 방법에 비해 46.9% 감소되었다.

• 비파괴검사학회지
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• 제35권2호
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• pp.134-140
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• 2015

전통적인 뜸 요법은 시술자의 주관적이고 정성적인 치료로 인해 환자의 피부에 강한 열 자극이 가해져 심한 통증을 주거나 화상을 입히는 문제점을 갖고 있다. 이러한 한계를 극복하기 위한 대안으로 객관성과 정량화가 가능한 초음파를 활용한 뜸 자극 치료기술이 주목받고 있다. 하지만, 초음파 에너지를 열에너지로 변환하기 위해서는 초음파 치료기기의 음향렌즈에 따른 초음파 집속 특성 분석이 필요하다. 따라서, 본 논문에서는 초음파 집속 특성에 영향을 주는 음향렌즈의 형태(오목렌즈, 계단형 렌즈)에 따른 집속 시뮬레이션을 통해 각 렌즈의 음압 분포, 음압의 크기, 초점의 위치를 계산하였으며, 설계한 렌즈를 제작하여 음압 측정 실험을 통해 음향렌즈의 집속 성능을 검증하였다.