• Journal of Microbiology
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• 제41권4호
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• pp.320-326
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• 2003

In order to identify Vibrio vulnificus in the Yellow Sea near Gunsan, Korea during the early and late summers, the efficiency of the real-time quantitative TaqMan PCR was compared to the efficiency of the conventional PCR and Biolog identification system^TM. Primers and a probe were designed from the hemolysin/cytolysin gene sequence of V. vulnificus strains. The number of positive detections by real-time quantitative TaqMan PCR, conventional PCR, and the Biolog identification system from seawater were 53 (36.8%), 36 (25%), and 10 strains (6.9%), respectively, among 144 samples collected from Yellow Sea near Gunsan, Korea. Thus, the detection method of the real-time quantitative TaqMan PCR assay was more effective in terms of accuracy than that of the conventional PCR and Biolog system. Therefore, our results showed that the real-time TaqMan probe and the primer set developed in this study can be applied successfully as a rapid screening tool for the detection of V. vulnificus.

• 한국자기공명학회논문지
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• 제20권3호
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• pp.87-94
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• 2016

Although NMR technique has been using in many areas of chemistry, its merit on quantitative analysis seems not to acknowledge greatly because of the many inferior intrinsic aspects, particularly its sensitivity. Recently, new NMR techniques, high-field NMR, and demands for cutting edge techniques of analysis, however, seem to change the role of NMR spectroscopy in this field. This review shows the application of NMR development in quantitative analysis and will discuss the basic idea, limitations, and pitfalls. Then it will show you several successful applications applied in quantitative analysis and you will see how useful and accurate method it is.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2006년도 하계학술발표대회 논문집
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• pp.460-467
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• 2006

Some large accidents in tunnels in recent years, such as Mont Blanc, Gotthard and Tauern tunnels, have lead to an increasing attention for tunnel safety and necessity of tool for quantitative risk assesment of road tunnel. And the purpose of this study is to develop the quantitative risk assesment tool for the application of road tunnel. The objectives of this paper are as follows : (1) analyze of traffic accident rates in tunnel, (2) make out scenario for fire accidents, (3) develop the evacuation model and FED calculation model, (4) Present the results from quantitative risk assesment for the model tunnel according with the fire heat release rates and distances of cross passage.

• 동의생리병리학회지
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• 제31권2호
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• pp.145-152
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• 2017

The purpose of this study was to investigate the clinical research of Cold-Heat of pattern identification(PI) focused on quantitative indicators for general people in Korea, through the review of OASIS, KISS, DBpia, NDSL database. Ten clinical researches were analyzed in this study. These research gradually started from 2004 to 2009 but there was no research after 2010. Diagnosis of Cold-Heat PI all was done through a questionnaire, 7 or most researches had not used the intervention methods. Quantitative indicators related to the Cold-Heat PI mentioned in the selected 10 researches are seven, these indicators include heart rate variability(HRV), electroencephalogram, Yangdorak, respiratory index, electrogastrography, acoustic analysis index, western health test index. Related to the HRV were 3 researches, but correlation of significant indicators was a few levels(R=0.026-0.090). So far, studies regarding at the quantitative indicators of the prior the Cold-Heat PI were few, and the results of the study showed that low statistical precision, a lack of biological basis. Refer to the current state, in the future, we expect that research of quantitative indicators of scientific evidence-based through calculated and precise research concerning Cold-Heat PI be regarded as important in Korean Medicine.

• 대기
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• 제25권2호
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• pp.283-294
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• 2015

For urban flash flood simulation, we need the higher resolution radar rainfall than radar rainfall of KMA, which has 10 min time and 1km spatial resolution, because the area of subbasins is almost below $1km^2$. Moreover, we have to secure the high quantitative accuracy for considering the urban hydrological model that is sensitive to rainfall input. In this study, we developed the quantitative precipitation estimation (QPE), which has 250 m spatial resolution and high accuracy using KMA AWS and SK Planet stations with Mt. Gwangdeok radar data in Seoul area. As the results, the rainfall field using KMA AWS (QPE1) is showed high smoothing effect and the rainfall field using Mt. Gwangdeok radar is lower estimated than other rainfall fields. The rainfall field using KMA AWS and SK Planet (QPE2) and conditional merged rainfall field (QPE4) has high quantitative accuracy. In addition, they have small smoothed area and well displayed the spatial variation of rainfall distribution. In particular, the quantitative accuracy of QPE4 is slightly less than QPE2, but it has been simulated well the non-homogeneity of the spatial distribution of rainfall.

• 한국신뢰성학회지:신뢰성응용연구
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• 제16권2호
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• pp.134-146
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• 2016

Purpose: Many domestic companies have to make out quantitative evaluation table in their proposal when they conduct the software R&D project. However, most of companies have a difficulty to select the evaluation items and criteria, also to derive a quantitative results. Therefore, we propose a method to derive the quantitative evaluation index by utilizing the ISO/IEC 9126-2. Methods: Analyzing ISO/IEC 9126-2, and we classify the quality metrics as high-classification and sub-classification for Web/App software, Embedded software and Installation software. Next, Conduct the metrics selection survey depending on importance and necessity. Then, carry out the case study. Verify the correspondence between evaluation items and criteria from original suggestion of company and from outcome by utilizing the ISO/IEC 9126-2 quality metrics. Results: It is possible to classify into two metrics, one for common software or one another for only special software. Furthermore, there is quality metrics that is more important and more necessary depending upon characteristics of the software. Conclusion: ISO/IEC 9126-2 quality metrics can be used to make an evaluation items and criteria for quantitative evaluation table of software product.

• 디지털산업정보학회논문지
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• 제14권3호
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• pp.115-125
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• 2018

In order to examine the ways to improve the efficiency of R&D investment, this paper presents analysis on both quantitative and qualitative efficiency of R&D projects. As Korea's R&D investment has significantly increased in recent years, the efficiency of R&D investment has attracted attention. In this paper, a Data Envelopment Analysis(DEA) method is used to construct models for quantitative efficiency and qualitative efficiency analysis. Based on a cases of agricultural R&D projects of Korea, the efficiency of national R&D projects were analyzed and their quantitative and qualitative efficiencies are compared. As a result, statistically significant difference between quantitative and qualitative efficiency was found. Also, characteristics of Decision Making Units(DMUs) which can influence both quantitative and qualitative efficiency were identified. In particular, the stage of a R&D project has significant impact on R&D efficiency. This study suggests that in order to enhance R&D efficiency both quantitative and qualitative nature of outputs should be considered when measuring R&D efficiency.

• Mass Spectrometry Letters
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• 제13권4호
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• pp.139-145
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• 2022

Protein glycosylation is a common post-translational modification by non-template-based biosynthesis. In fungal biotechnology, which has great applications in pharmaceuticals and industries, the importance of research on fungal glycoproteins and glycans is accelerating. In particular, the importance of quantitative analysis of fungal glycans is emerging in research on the production of filamentous fungal proteins by genetic modification. Reliable mass spectrometry-based techniques for quantitative glycomics have evolved into chemical, enzymatic, and metabolic stable isotope labeling methods. In this study, we intend to expand quantitative glycomics by metabolic isotope labeling of glycans in Aspergillus niger, a filamentous fungus model, by the MILPIG method. We demonstrate that incubation of filamentous fungi in a culture medium with carbon-13 labeled glucose (1-¹³C₁) efficiently incorporates carbon-13 into N-linked glycans. In addition, for quantitative validation of this method, light and heavy glycans are mixed 1:1 to show the performance of quantitative analysis of various N-linked glycans simultaneously. We have successfully quantified fungal glycans by MILPIG and expect it to be widely applicable to glycan expression levels under various biological conditions in fungi.

• Journal of Forest and Environmental Science
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• 제39권3호
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• pp.119-127
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• 2023

In this study, quantitative models were applied to case parks to estimate the carbon reduction by trees, which was compared and analyzed at the tree and park levels. At the tree level, quantitative models of carbon storage and uptake differed by up to 7.9 times, even for the same species and size. At the park level, the carbon reduction from quantitative models varied by up to 3.7 times for the same park. In other words, carbon reduction by quantitative models exhibited considerable variation at the tree and park levels. These differences are likely due to the use of different growth environment coefficients and annual diameter at breast height growth rates and the overestimation of carbon reduction due to the substitution of the same genus and group model for each tree species. Extending the annual carbon uptake per unit area of the case park to the total park area of Chuncheon a carbon uptake ranging from a minimum of 370.4 t/yr and a maximum of 929.3 t/yr, and the difference can reach up to 558.9 t/yr. This is equivalent to the carbon emissions from the annual household electricity consumption of approximately 2,430 people. These results suggest that the indiscriminate application of quantitative models to estimate carbon reduction in urban trees can lead to significant errors and deviations in estimating carbon storage and uptake in urban greenspaces. The findings of this study can serve as a basis for estimating carbon reduction in urban greening research, projects, and policies.

• 시스템엔지니어링학술지
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• 제20권spc1호
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• pp.1-11
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• 2024

Today, the importance of test and evaluation of defense systems is increasing day by day. In performing efficient defense systems test works, process improvement based on quantitative management is essential. The purpose of this paper is to present the results of process improvement for the defense systems test works of the test organization based on quantitative management activities. As a methodology to confirm process improvement performance, the 'MPM(Managing Performance and Measurement)' practice area of the CMMI model was applied. The quantitative management model for defense systems test works was developed so that it could be practically applied not only to the entire test organization but also to the organization at the department level that actually performs the test work. Finally, the application cases of the quantitative management model for defense system test works and the results of process improvement were described.