• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.193-201
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• 2010

A specific and rapid real-time PCR assay for detecting Ralstonia solanacearum in horticultural soil and plant tissues was developed in this study. The specific primers RSF/RSR were designed based on the upstream region of the UDP-3-O-acyl-GlcNAc deacetylase gene from R. solanacearum, and a PCR product of 159 bp was amplified specifically from 28 strains of R. solanacearum, which represent all genetically diverse AluI types and all 6 biovars, but not from any other nontarget species. The detection limit of $10^2\;CFU/g$ tomato stem and horticultural soil was achieved in this real-time PCR assay. The high sensitivity and specificity observed with field samples as well as with artificially infected samples suggested that this method might be a useful tool for detection and quantification of R. solanacearum in precise forecast and diagnosis.

• 분석과학
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• 제25권1호
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• pp.33-38
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• 2012

염화물 고정화능력의 중요성에도 불구하고 고정화능력을 측정하는 것은 어려우며 특히, 이미 건설되어 공용중인 구조물에서는 더욱 어렵다. 실제로 고정염화물을 측정하는 과정은 온도, 시료의 분말도, 공극수 추출기법 등과 같은 환경적 요인에 의해 큰 영향을 받는다. 본 연구에서는 X-선 회절분석법(XRD; X-ray diffraction)을 이용하여 콘크리트 내 염화물 이온의 고정화능력을 정량화하는 방법에 대해 중점을 두고 연구를 진행하였다. 염화물의 고정능력 isotherm은 물/시멘트비, 양생기간, 결합재 종류에 영향을 받으며 Langmuir isotherm 을 통하여 일단 결정된 고정염화물(Friedel 염)은 XRD를 이용하여 동시에 분석을 진행한다. XRD 그래프에서 나타난 고정염화물량의 피크 강도와 측정된 고정염화물의 총량의 관계를 결정한다. 결과적으로 양생기간이 증가될수록, 물/시멘트비가 감소될수록 고정화 능력은 증가하였다.

• 한국환경과학회지
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• 제17권8호
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• pp.891-898
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• 2008

This study was conducted to develop the effects of site environmental factors on estimation of site index function for Chamaecyparis obtusa Endlicher stands. We derived nonlinear growth equation and the draw site index curves by applying this estimated equation. This study with Chapman-Richards function showed significant P-value which was less then 0.0001 and $R^2$ value 0.5947. This study was conducted to develop the feasible site index equation of Chamaecyparis obtusa Endlicher. For the table, the data of 82 sample areas that were thought to be without errors among the data of Chamaecyparis obtusa Endlicher sample area located on the value-oriented forest location chart were used and estimated. After analyzing the quantification method I based on 13 environmental factors to develop the score table for the site-index estimation of Chamaecyparis obtusa Endlicher, $R^2$ value of the model was 0.7555. It has been analyzed that the scope value of Soil moisture in horizon A was 7.5045, that of total soil depth was 6.3896, that of topography was 5.3471, that of slope was 4.7000 and that of aspect was 3.2038. After analyzing the partial correlation to examine the factors that affected most the site-index of Chamaecyparis obtusa Endlicher, it has been noted that the partial correlation of climatic zone was 0.4987, which was highest, and it was followed by Soil moisture in horizon A (0.4592), slope (0.4537), topography (0.3299) and total soil depth (0.1035). As a result of conducting the significance test for partial correlation, it has been found that topography, climatic zone, parent rock, slope, altitude, aspect, Soil moisture in horizon A, soil hardness in horizon A and total soil depth were recognized significant with 1% of significance level and sedimentary type and soil texture in horizon A were recognized significant with 5% of significance level.

• 대한핵의학회지
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• 제38권3호
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• pp.241-252
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• 2004

목적: 다수의 뇌 자료를 기반으로 구성된 통계적 화률뇌지도는 복잡하고 개인적인 편차가 큰 뇌의 형태학적, 기능적 특성을 분석하는데 유용하다. 특히 최근에 한국인의 정상 MR 영상을 기반으로 한 구조적 기능적 뇌 표준판과 구조적 확률뇌지도가 완성되었으며. 부검뇌의 조직절편을 활용한 세포구축학적 확률뇌지도가 도입되었다. 이 연구에서는 이들 자료를 활용하여 뇌 영상의 국소계수를 객관적으로 측정하기 위한 정량화 기법을 개발하였으며, 이를 이용하여 정상 한국인의 뇌 영상자료를 분석하였다. 대상 및 방법 : T1 MRI와 FDG PET에 대한 뇌 표준판을 성별, 연령별로 개발하였으며, 한국인 정상 MR 영상으로 만들어진 89개 뇌 영역의 구조적 확률뇌지도와 독일 율리히 연구센터에서 도입한 13개 브로드만 영역에 대한 세포구축학적 확률뇌지도를 뇌 표준판 위로 각각 비선형 변환하였다. 확률뇌지도에 정의된 각 뇌 영상의 국소계수는 확률에 의한 가중평균 또는 가중합으로 구하였다. 확률뇌지도를 이용한 예비 연구로 정상 노화에 따른 포도당대사의 변화가 대상회 내에서 전 후 위치에 따라 다르게 나타나는 가를 조사하였다. 결과: SPM과의 연계된 사용을 고려하여 Matlab 상에서 작성된 정량화 프로그램은 20초 미만에 1개의 공간정규화된 영상을 처리할 수 있었다. 대상회 포도당 대사에 대한 분석에서 후대상회에 대한 문측-전대상회 및 미측-전대상회의 계수 비는 정상 노화에 따라 매우 유의하게 감소하였다. 즉. 문측-전대상회/후대상회는 매 10년마다 3.1%씩 감소하였으며($P<10^{-11}$, r=0.81) 미측-전대상회/후대상회는 1.7%씩 감소하였다($P<10^{-8}$, r=0.72). 문측-전대상회와 미측-전대상회의 계수비 감소 역시 유의하였다(P<0.0005. r=0.52, 1.5%/year). 결론: 서양인 뇌에 기반한 확률뇌지도는 그동안 제한적으로만 사용되어 왔으나 한국인 뇌 표준판과 확률뇌지도 및 이 연구에서 개발한 정량화 기법은 일반에 공개하기로 하여 더욱 널리 쓰이게 되면 국내 뇌 연구 활성화에 기여할 것으로 기대 된다.

• Natural Product Sciences
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• 제23권2호
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• pp.84-91
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• 2017

This study proposes a sensitive and selective liquid chromatography-electrospray ionization tandem mass spectrometry method of efficiently assessing the quality of a traditional herbal medicine called Yeonggyechulgam-tang (YGCGT). The following compounds 1 - 11, namely, liquiritin apioside (1), liquiritin (2), liquiritigene (3), coumarin (4), cinnamic acid (5), cinnamaldehyde (6), glycyrrhizin (7), atractylenolide III (8), atractylenolide II (9), atractylenolide I (10), and pachymic acid (11) were separated on a UPLC BEH $C_{18}$ column ($2.1{\times}100mm$, $1.7{\mu}m$) at a column temperature of $45^{\circ}C$ eluted with a gradient condition of 0.1% (v/v) formic acid in distilled water and acetonitrile. The correlation coefficient of the calibration curve of the eleven constituents was ${\geq}0.9936$. The limits of detection and quantification of the compounds 1 - 11 were 0.06 - 4.73 ng/mL and 0.17-14.20 ng/mL, respectively. Using this analytical method, the compound 11 in lyophilized YGCGT decoction extract was not detected, while the compounds 1 - 10 were detected 0.13-166.43 mg/g.

• Structural Engineering and Mechanics
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• 제72권5호
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• pp.585-595
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• 2019

Foundations are a vital part of structures. Over time, the foundations can deteriorate due to unforeseen overloads and/or settlements, resulting in the appearance of cracks in the concrete. These cracks produce changes in the static and dynamic behavior of the affected foundation, which alter its load carrying capacity. In this work, non-destructive techniques of relative simplicity of application are presented for the detection, location, and quantification of damage, using numerical models, solved with the finite element method and Power Series. For this, two types of parameters are used: static (displacement and elastic curvature) and dynamics (natural frequencies). In the static analysis, the damage detection is done by means of a finite elements model representing a beam supported on an elastic foundation with a discrete crack that varies in length and location. With regard to dynamic analysis, the governing equations of the model are presented and a method based on Power Series is used to obtain the solution for a data set, which could be the Winkler coefficient, the location of the crack or the frequency. In order to validate the proposed methodologies, these techniques are applied to data obtained from laboratory tests.

• KSBB Journal
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• 제30권4호
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• pp.141-147
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• 2015

The convenient determination of methylsulfonylmethane (MSM) for a commercially available dietary supplement was developed using gas chromatography (GC)-flame ionization detector (FID). Chromatography was performed on a capillary column ($0.32mm\;I.D{\times}30m$, $0.25{\mu}m$) coated with dimethylpolysiloxane using diethylene glycol methyl ether as an internal standard. The performance characteristics of GC were evaluated in terms of selectivity, linearity, precision, accuracy, recovery, limit of detection (LOD) and limit of quantification (LOQ). The calibration curve was highly linear (the coefficient of determination: 0.9979) within the concentration range of $10.0{\sim}800.0{\mu}g/mL$ for MSM. The recoveries for three fortified concentrations were 96.7~97.1%, 96.6~97.3% and 96.8~97.2%, respectively. The LOD and LOQ of the method were $0.29{\mu}g/mL$ and $0.97{\mu}g/mL$, respectively. All obtained results were acceptable according to the guidelines of the Association of Official Analytical Chemists for dietary supplements. Thus, the validated analytical method using the GC-FID system is suitable for the determination of MSM in dietary supplement formulations for quality control.

• Earthquakes and Structures
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• 제8권4호
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• pp.915-934
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• 2015

Within the context of Structural Health Monitoring (SHM), it is often the case that structural systems are described by uncertainty, both with respect to their parameters and the characteristics of the input loads. For the purposes of system identification, efficient modeling procedures are of the essence for a fast and reliable computation of structural response while taking these uncertainties into account. In this work, a reduced order metamodeling framework is introduced for the challenging case of nonlinear structural systems subjected to earthquake excitation. The introduced metamodeling method is based on Nonlinear AutoRegressive models with eXogenous input (NARX), able to describe nonlinear dynamics, which are moreover characterized by random parameters utilized for the description of the uncertainty propagation. These random parameters, which include characteristics of the input excitation, are expanded onto a suitably defined finite-dimensional Polynomial Chaos (PC) basis and thus the resulting representation is fully described through a small number of deterministic coefficients of projection. The effectiveness of the proposed PC-NARX method is illustrated through its implementation on the metamodeling of a five-storey shear frame model paradigm for response in the region of plasticity, i.e., outside the commonly addressed linear elastic region. The added contribution of the introduced scheme is the ability of the proposed methodology to incorporate uncertainty into the simulation. The results demonstrate the efficiency of the proposed methodology for accurate prediction and simulation of the numerical model dynamics with a vast reduction of the required computational toll.

• 대한한의진단학회지
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• 제10권2호
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• pp.54-66
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• 2006

Objective : To review the method, parameters, system of electrogastrography and to study its oriental medical application. Method : We investigate the research results through internet search engines such as Pubmed, China National Knowledge Infrastructure. Conclusion : Electrogastrography is believed to be of great use to the verification of treatment effect of herbal medicine that is used in internal medicine and neuropsychiatry, along with the objectification and quantification of diagnosis of oriental medicine related to gastric peristalsis and balance of autonomic nervous system. I would therefore like to conclude that there should be continued study and active application of electrogastrography in the wide variety of oriental medicine.

• 대한한의진단학회지
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• 제13권1호
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• pp.45-53
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• 2009

Background and purpose : We previously developed questionnaire of Guibi-tang symtom on the Delphi method through the pathogenesis analysis. But developed a questionnaire was not verified in the clinical. So, to ensure objectivity, quantification and validity, verification is needed for questionnaire items before applying a clinical. On this study, we looked at whether questionnaire items had been validity in the clinical. Methods : Participants of this study were outpatients in eleven clinics. The resources were collected from 200 patients. SPSS 15.0 for Windows was used for statistical analysis : reliability analysis, factor analysis were used to verify the results Results and Conclusions : 16 items were selected through reliability analysis perforfed on about 22 items. After factor analysis, we have four component. Veryfy research of the Guibi-tang Patternization Questionnaire is needed in the future. Also I think that research should proceed about a lot of people.