• Journal of Korean Society of Transportation
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• v.28 no.3
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• pp.39-49
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• 2010

Provision of in-vehicle warning information is of keen interest since it can be effectively used to prevent traffic accident on the road. This study evaluates the effectiveness of information provision modalities based on kansei engineering. Various warning information scenarios using different modalities are devised for the evaluation. Statistical data analysis techniques including factor analysis, correlation analysis, and the general linear model are used to assess the user's affect for information modalities. The evaluation result shows that the provision of visual information consisted of 'text and pictogram' leads to higher understandability. The combination of beep sound and voice message' was identified as a more effective modality for auditory warning. In addition, the red color for the blinking warning signal was preferred by users.

• The Korean Journal of Food And Nutrition
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• v.14 no.6
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• pp.521-526
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• 2001

A method for the simultaneous and precise determination of lactone form and acid form monacolin K in red yeast rice by HPLC was developed in this study. The standard of acid form monacolin K was prepared by alkaline hydrolysis of its lactone form, which was purchased from Sigma company. The optimum HPLC system for the separation and quantification of acid form and lactone form monacolin K is based on the reversed-phase column, and the acidified mobile phase consisting of acetonitrile : 0.1% trifluoroacetic acid (TFA) water soln : 62 :38, the low limit detection amount was 5 ng (i.e.10 $\mu$l injection of 0.5 $\mu\textrm{g}$/ml) . And the optimal extracting system for monacolins in red rice was also presented here.

• Korean Journal of Pharmacognosy
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• v.38 no.2 s.149
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• pp.192-196
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• 2007

A rapid and practical HPLC assay was developed for quantitative analysis of saponins in Platycodi Radix. Seven saponin components in Platycodi Radix, i.e., deapioplatycoside E, platycoside E, deapioplatycodin D$_3$, platycodin D$_3$, polygalacin D$_2$, platycodin D$_2$ and platycodin D were successfully resolved on C18 column and detected by ELSD. Standard curves were linear over the concentration range 1 ${\sim}$2,000 ${\mu}$g/ml (r$^2$>0.992). Intra- and inter-day coefficients for variation of seven saponin components were < 10% and limit of quantification of them were around 0.7${\sim}$1.5 ${\mu}$g/ml, respectively. Using this method, contents of seven saponins in various plant materials under different cultivating conditions were estimated.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1114-1119
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• 2004

The biofilms on pipe walls in water distribution systems are of interest since they can lead to chlorine demand, coliform growth, pipe corrosion, and water taste and odor problems. As such, the study described in this paper is part of an AWWARF and Tampa Bay Water tailored collaboration project to determine the effect of blending different source waters on the water quality in various distribution systems. The project was based on 18 independent pilot distribution systems (PDS), each being fed by a different water blend (7 finished waters blended in different proportions). The source waters compared were groundwater, surface water, and brackish water, which were treated in a variety of pilot distribution systems, including reverse osmosis (RO) (desalination), both membrane and chemical softening, and ozonation-biological activated carbon (BAC), resulting in a total of 7 different finished waters. The observations from this study consistently demonstrated that unlined ductile iron was more heavily colonized by a biomass than galvanized steel, lined ductile iron, and PVC (in that order) and that the fixed biomass accumulation was more influenced by the nature of the supporting material than by the water quality (including the secondary residual levels). However, although the bulk liquid water cultivable bacterial counts (i.e. heterotrophic plate counts or HPCs) did not increase with a greater biofilm accumulation, the results also suggested that high HPCs corresponded to a low disinfectant residual more than a high biofilm inventory. Furthermore, temperature was found to affect the biofilms, plus the AOC was important when the residual was between 0.6 and 2.0 mg $Cl_2/l$. An additional aspect of the current study was that the potential of the exoproteolytic activity (PEPA) technique was used along with a traditional so-called destructive technique in which the biofilm was scrapped off the coupon surface, resuspended, and cultivated on an R2A agar. Both techniques indicated similar trends and relative comparisons among the PDSs, yet the culturable biofilm values for the traditional method were several orders of magnitude lower than the PEPA values.

• Bulletin of the Korean Chemical Society
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• v.28 no.7
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• pp.1187-1194
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• 2007

The three major active isoflavonoids (calycosin-7-O-β -glucoside, isomucronulatol 7-O-β-glucoside, formononetin) and two main saponins (astragaloside I, astragaloside IV) in an extract of Radix Astragali were determined using rapid, sensitive, reliable HPLC/UV and LC-ESI-MS/MS methods. The separation conditions employed for HPLC/UV were optimized using a phenyl-hexyl column (4.6 × 150 mm, 5 μm) with the gradient elution of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 230 nm. The specificity of the peaks was determined using a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization (ESI) source that was operated in multiple reaction monitoring (MRM) in the positive mode. These methods were fully validated with respect to the linearity, accuracy, precision, recovery and robustness. The HPLC/UV method was applied successfully to the quantification of three major isoflavonoids in the extract of Radix Astragali. The results indicate that the established HPLC/UV and LC-ESI-MS/MS methods are suitable for the quantitative analysis and quality control of multi-components in Radix Astragali.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.780-786
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• 2005

This study presents a non-video, non-invasive, automatic, on-site monitoring system the system employs ultrasonic transducers to detect behavior in sows before, during and after parturition. An ultrasonic transmitting/receiving (T/R) circuit of 40 kHz was mounted above a conventional parturition bed. The T/R units use ultrasonic time-of-flight (TOF) ranging technology to measure the height of the confined sows at eight predetermined locations. From this data, three momentary postures of the sow are determined, characterized as standing-posture (SP), lateral-lying-posture (LLP) and sitting posture (STP). By examining the frequencies of position switch Stand-Up-Sequence (SUS) between standing-posture (SP), lateral-lying-posture (LLP) and sitting-posture (STP) rate can be determined for the duration of the sow' confinement. Three experimental pureblooded Landrace sows undergoing normal gestation were monitored for the duration of confinement. In agreement with common observation, the sows exhibited increased restlessness as parturition approached. Analysis of the data collected in our study showed a distinct peak in Stand-Up-Sequence (SUS, i.e. the transition from lying laterally to standing up ) and sitting-posture (STP) rate approximately 12 h prior to parturition, the observed peak being 5 to 10 times higher than observed on any other measurement day. It is concluded that the presented methodology is a robust, low-cost, lowlabor method for the continuous remote monitoring of sows and similar large animals for parturition and other behavior. It is suggested that the system could be applied to automatic prediction of sow parturition, with automatic notification of remote management personnel so human attendance at birth could reduce rates of sow and piglet mortality. The results of this study provide a good basis for enhancing automation and reducing costs in large-scale sow husbandry and have applications in the testing of various large mammals for the effects of medications, diets, genetic modifications and environmental factors.

• Journal of Ocean Engineering and Technology
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• v.32 no.4
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• pp.244-252
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• 2018

This paper provides quantification of the effects of the turbulence model and grid refinement on the analysis of tip vortex flows by using the RANS(Reynolds averaged Navier-Stokes) method. Numerical simulations of the tip vortex flows of the NACA $66_2$-415 elliptic hydrofoil were conducted, and two turbulence models for RANS closure were tested, i.e., the Realizable $k-{\varepsilon}$ model and the Reynolds stress transport model. Numerical results were compared with available experimental data, and it was shown that the data for the Reynolds stress transport model that were computed on the finest grid system had better agreement in reproducing the development and propagation of the tip vortex. The Realizable $k-{\varepsilon}$ model overestimated the turbulence level in the vortex core and showed a diffusive behavior of the tip vortex. The tip vortex cavitation on the hydrofoil and its trajectory also showed good agreement between the current numerical results that were obtained using the Reynolds stress transport model and the results observed in the experiment.

• Nuclear Engineering and Technology
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• v.44 no.4
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• pp.421-428
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• 2012

Analog instrument and control systems in nuclear power plants have recently been replaced with digital systems for safer and more efficient operation. Digital instrument and control systems have adopted various fault-tolerant techniques that help the system correctly and safely perform the specific required functions regardless of the presence of faults. Each fault-tolerant technique has a different inspection period, from real-time monitoring to monthly testing. The range covered by each faulttolerant technique is also different. The digital instrument and control system, therefore, adopts multiple barriers consisting of various fault-tolerant techniques to increase the total fault detection coverage. Even though these fault-tolerant techniques are adopted to ensure and improve the safety of a system, their effects on the system safety have not yet been properly considered in most probabilistic safety analysis models. Therefore, it is necessary to develop an evaluation method that can describe these features of digital instrument and control systems. Several issues must be considered in the fault coverage estimation of a digital instrument and control system, and two of these are addressed in this work. The first is to quantify the fault coverage of each fault-tolerant technique implemented in the system, and the second is to exclude the duplicated effect of fault-tolerant techniques implemented simultaneously at each level of the system's hierarchy, as a fault occurring in a system might be detected by one or more fault-tolerant techniques. For this work, a fault injection experiment was used to obtain the exact relations between faults and multiple barriers of faulttolerant techniques. This experiment was applied to a bistable processor of a reactor protection system.

• Journal of Microbiology and Biotechnology
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• v.20 no.10
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• pp.1463-1470
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• 2010

E. coli has long been widely used as a host system for the manufacture of recombinant proteins intended for human therapeutic use. When considering the impurities to be eliminated during the downstream process, residual host cell DNA is a major safety concern. The presence of residual E. coli host cell DNA in the final products is typically determined using a conventional slot blot hybridization assay or total DNA Threshold assay. However, both the former and latter methods are time consuming, expensive, and relatively insensitive. This study thus attempted to develop a more sensitive real-time PCR assay for the specific detection of residual E. coli DNA. This novel method was then compared with the slot blot hybridization assay and total DNA Threshold assay in order to determine its effectiveness and overall capabilities. The novel approach involved the selection of a specific primer pair for amplification of the E. coli 16S rRNA gene in an effort to improve sensitivity, whereas the E. coli host cell DNA quantification took place through the use of SYBR Green I. The detection limit of the real-time PCR assay, under these optimized conditions, was calculated to be 0.042 pg genomic DNA, which was much higher than those of both the slot blot hybridization assay and total DNA Threshold assay, where the detection limits were 2.42 and 3.73 pg genomic DNA, respectively. Hence, the real-time PCR assay can be said to be more reproducible, more accurate, and more precise than either the slot blot hybridization assay or total DNA Threshold assay. The real-time PCR assay may thus be a promising new tool for the quantitative detection and clearance validation of residual E. coli host cell DNA during the manufacturingprocess for recombinant therapeutics.

• Journal of Korean Society of Forest Science
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• v.19 no.1
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• pp.49-54
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• 1973

Surveys of stock volume on steep and vast expanse of mountains, involves various difficulties. And it is extremely uneconomical in forest management point of view, to spend lots of time and man power for surveying such tree volume as the value is much cheaper in comparison with volume and weight. Therefore, a stand volume table estimate easely stock volume per hectare basis from aerial photographs was prepared and correlations to stand volume among factors affecting tree volumation, were studied. Data were 114 places selected from planted Korean white pine, Pinus koreiensis Sieb. et Zucc. stands in Kwangnung Experiment Forest and were computed and analysed by the means of the quantification in the multi-variate statistical analysis. Electronic Data Processing System was applied for data processing at Korean Instiute of Science and Technology. Coefficients of multiple correlations of stand volume table was ranged 0.85~0.88.