• Journal of Pharmacopuncture
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• v.26 no.4
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• pp.307-318
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• 2023

Objectives: Bacterial biofilm is regarded as a significant threat to the production of safe food and the arise of antibiotic-resistant bacteria. The objective of this investigation is to evaluate the quorum sensing inhibitory effect of Nepeta curviflora methanolic extract. Methods: The effectiveness of the leaves at sub-inhibitory concentrations of 2.5, 1.25, and 0.6 mg/mL on the virulence factors and biofilm formation of P. aeruginosa was evaluated. The effect of N. curviflora methanolic extract on the virulence factors of P. aeruginosa, including pyocyanin, rhamnolipid, protease, and chitinase, was evaluated. Other tests including the crystal violet assay, scanning electron microscopy (SEM), swarming motility, aggregation ability, hydrophobicity and exopolysaccharide production were conducted to assess the effect of the extract on the formation of biofilm. Insight into the mode of antiquorum sensing action was evaluated by examining the effect of the extract on the activity of N-Acyl homoserine lactone (AHL) and the expression of pslA and pelA genes. Results: The results showed a significant attenuation in the production of pyocyanin and rhamnolipid and in the activities of protease and chitinase enzymes at 2.5 and 1.25 mg/mL. In addition, N. curviflora methanolic extract significantly inhibited the formation of P. aeruginosa biofilm by decreasing aggregation, hydrophobicity, and swarming motility as well as the production of exopolysaccharide (EPS). A significant reduction in AHL secretion and pslA gene expression was observed, indicating that the extract inhibited quorum sensing by disrupting the quorum-sensing systems. The quorum-sensing inhibitory effect of N. curviflora extract appears to be attributed to the presence of kaempferol, quercetin, salicylic acid, rutin, and rosmarinic acid, as indicated by LCMS analysis. Conclusion: The results of the present study provide insight into the potential of developing anti-quorum sensing agents using the extract and the identified compounds to treat infections resulting from quorum sensing-mediated bacterial pathogenesis.

• Proceedings of the Korea Information Processing Society Conference
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• 2003.05a
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• pp.173-176
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• 2003

최근 분산 컴퓨팅 환경에서 데이터와 서비스의 복제는 통신비용의 감소, 데이터 가용성 증가, 그리고 단일 서버의 병목현상을 피하기 위해 필수적이다. 기존의 대표적인 복제 프로토콜로 네트워크를 논리적으로 구성하는 Tree quorum 프로토콜이 있다 Tree quorum프로토콜은 최선의 경우 가장 우수한 읽기 성능을 보이는 반면 트리의 높이가 증가할수록 노드의 수가 기하급수적으로 증가한다는 단점을 가지고 있다. 따라서 본 논문에서는 Tree quorum 프로토콜의 장점을 가지며, 급격한 노드 증가에 따른 성능 저하 문제를 해결하기 위한 가상 트리 프로토콜을 제안한다. 제안된 가상 트리 프로토콜은 안정된 삼각형 구조의 노드 구성과 가상 구조를 통한 Quorum 프로토콜의 사용으로 Tree quorum 프로토콜에 비해 적은 읽기 비용을 가지며, 적은 수의 노드 구성에서도 높은 읽기 가용성을 갖는다.

• Journal of Korea Multimedia Society
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• v.20 no.8
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• pp.1369-1378
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• 2017

Pub/Sub (Publish/Subscribe) paradigm is a simple and easy to use model for interconnecting applications in a distributed environment. In general, subscribers register their interests in a topic or a pattern of events and then asynchronously receive events matching their interest, regardless of the events' publisher. In order to build a low latency lightweight pub/sub system for Internet of Things (IoT) services, we propose a GQSFPS (Group Quorum System-based Fog Pub/Sub) system that is a core component in the event-driven service oriented architecture framework for IoT services. The GQSFPS organizes multiple installed pub/sub brokers in the fog servers into a group quorum based P2P (peer-to-peer) topology for the efficient searching and the low latency accessing of events. Therefore, the events of IoT are cached on the basis of group quorum, and the delay-sensitive IoT applications of edge devices can effectively access the cached events from group quorum fog servers in low latency. The performance of the proposed GQSFPS is evaluated through an analytical model, and is compared to the GQPS (grid quorum-based pud/sub system).

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.401-407
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• 2014

Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ${\Delta}luxS$ (ECM101) and ${\Delta}luxS{\Delta}relA{\Delta}spoT$ (ECM201), and the ${\Delta}luxS$ complement strain to ECM201 (ECM202) were created from EHEC O157:H7 EDL933 to investigate how the regulatory systems interact. The phenotypic changes of the mutant strains were characterized and compared with the wild type. The mutant strains exhibited no obvious growth defects, although acid resistance and cellular cytotoxicity were decreased significantly in all the mutant strains. Phenotypic characterization revealed that mutations in the stringent response system (ECM201 and ECM202) influenced the metabolic (defective utilization of arabinose and L-sorbose) and enzymatic activities (decreased trypsin activity, and increased ${\alpha}$-glucosidase activity). In contrast, the quorum sensing system mutant (ECM101) did not display these phenotypes. The motility of the quorum sensing system mutant (ECM101) was unchanged, but mutation in the stringent response system influenced the motility. Our results suggest that quorum sensing interacts with the stringent response regulation system.

• Journal of Pharmacopuncture
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• v.25 no.1
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• pp.37-45
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• 2022

Objectives: The quorum-sensing-inhibitory and anti-biofilm activities of the methanol extract of E. globulus leaves were determined against clinically isolated multidrug-resistant Pseudomonas aeruginosa. Methods: The preliminary anti-quorum-sensing (AQS) activity of eucalyptus was investigated against a biosensor strain Chromobacterium violaceum ATCC 12472 (CV12472) by using the agar well diffusion method. The effect of sub-minimum inhibitory concentrations (sub-MICs) of the methanol extract of eucalyptus on different quorum-sensing-regulated virulence factors, such as swarming motility, pyocyanin pigment, exopolysaccharide (EPS), and biofilm formation, against clinical isolates (CIs 2, 3, and 4) and reference PA01 of Pseudomonas aeruginosa were determined using the swarm diameter (mm)-measurement method, chloroform extraction method, phenol (5%)-sulphuric acid (concentrated) method, and the microtiter plate assay respectively, and the inhibition (%) in formation were calculated. Results: The preliminary AQS activity (violacein pigment inhibition) of eucalyptus was confirmed against Chromobacterium violaceum ATCC 12472 (CV12472). The eucalyptus extract also showed concentration-dependent inhibition (%) of swarming motility, pyocyanin pigment, EPS, and biofilm formation in different CIs and PA01 of P. aeruginosa. Conclusion: Our results revealed the effectiveness of the E. globulus extract for the regulation of quorum-sensing-dependent virulence factors and biofilm formation at a reduced dose (sub-MICs) and suggest that E. globulus may be a therapeutic agent for curing and controlling bacterial infection and thereby reducing the possibility of resistance development in pathogenic strains.

• Journal of KIISE:Computer Systems and Theory
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• v.31 no.9
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• pp.503-513
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• 2004

In large distributed systems, replications of data and service are needed to decrease communication cost, increase availability, and avoid single server bottleneck. Tree Quorum protocol is a representative replication protocol, which exploits a logical structure. Tree quorum protocol is one of the replication protocols allowing low read cost only in the best case, while the number of replicas exponentially increases as the level grows. In this paper, thus, we propose a new replication protocol, called symmetric tree protocol which efficiently solves the problem. The proposed symmetric tree protocol also requires much smaller read cost than previous protocols. We conduct cost and availability analysis of the protocols, and the proposed protocol displays comparable read availability to the tree protocol using much smaller number of nodes. Also, the symmetric tree protocol has much smaller response time than the logarithmic protocol.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1598-1606
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• 2007

A new series comprising 7 analogs of N-(sulfanyl ethanoyl)-L-HSL derivatives, 2 analogs of N-(fluoroalkanoyl)-$_L$-HSL derivatives, N-(fluorosulfonyl)-L-HSL, and 2,2-dimethyl butanoyl HSL were synthesized using a solid-phase organic synthesis method. Each of the 11 synthesized compounds was analyzed using NMR and mass spectroscopies, and molecular modeling studies of the 11 ligands were performed using SYBYL packages. Thereafter, a bacterial test was designed to identify their quorum-sensing inhibition activity and antifouling efficacy. Most of the synthesized compounds were found to be effective as quorum-sensing antagonists, where antagonist screening revealed that 10 among the 11 synthesized ligands were able to antagonize the quorum sensing of A. tumefaciens.

• Journal of dental hygiene science
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• v.24 no.2
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• pp.84-96
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• 2024

Background: Recent studies have elucidated the quorum-sensing mechanisms, biofilm formation, inter-pathogen interactions, and genes related to oral pathogens. This review aims to explore the recent expansion of drug targets against oral pathogens and summarize the current research on novel antibiotic substances derived from marine organisms that target oral pathogens. Methods: A comprehensive literature review summarized the novel mechanisms pertaining to quorum-sensing signal transmission systems, biofilm formation, and metabolite exchange in oral pathogens. The amino acid sequences of the 16 proteins identified as potential drug targets were systematically classified and compared across various oral microorganisms. Results: Through a literature review, we identified nine studies researching quorum sensing signaling inhibitors targeting oral pathogens. A comparison of the amino acid sequences of 16 potential drug targets in oral microorganisms revealed significant differences between oral pathogens and beneficial oral symbiotic microorganisms. These findings imply that it is possible to design drugs that can bind more selectively to oral pathogens. Conclusion: By summarizing the results of recent research on the signaling mechanisms that cause pathogenicity, new drug targets against oral pathogens were proposed. Additionally, the current status of developing new antibiotics for oral pathogens using recently developed quorum sensing inhibitors and natural products derived from marine organisms was introduced. Consequently, marine natural products can be used to develop drugs targeting new proteins in oral pathogens.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.7
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• pp.750-753
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• 2016

Quorum sensing (QS) is a bacterium-to-bacterium cell communication mechanism allowing bio-cell network construction but such mechanism is not well defined yet. We construct a QS-based multiple access network (MAN) and then numerically analyse its average uplink channel capacity as well as BER performance over diffusion-based 3-D molecular communication channels.

• Proceedings of the Korea Institutes of Information Security and Cryptology Conference
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• 2002.11a
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• pp.53-56
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• 2002

This paper discusses the quorum based algorithm for group mutual exclusion defined by Yuh-Jzer Joung. Group mutual exclusion[4,5,6] is a generalization of mutual exclusion that allows a resource to be shared by processes of the same group, but requites processes of different groups to use the resource in a mutually exclusive style. Joung proposed a quorum system, which he referred to as the surficial quorum system for group mutual exclusion and two modifications of Maekawa's algorithm[6]. He mentioned that when a process may belong to more than one group, the process must identify one of the groups it belongs when it wishes to enter CS(Critical Section). However, his solution didn't provide mechanism of identifying a group which maximizes the possibility to enter CS. In this paper, we provide a mechanism for identifying that each process belongs to which group.