• Journal of Korean Society on Water Environment
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• v.26 no.2
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• pp.268-278
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• 2010

This study derived the effectiveness analysis results of construction of wastewater treatment plant under climate change scenarios. Canadian Global Coupled Model (CGCM3) was used and A1B and A2 of Special Report on Emission Scenario (SRES) were selected. Regional climate change data for this application were downscaled by using Statistical Downscaling Model (SDSM) and the flow and BOD concentration durations were obtained by using Hydrological Simulation Program - Fortran (HSPF). The criteria for low flow and water quality were chosen as $Q_{99}$, $Q_{95}$, $Q_{90}$ and $C_{30}$, $C_{10}$, $C_1$. The numbers of days to satisfy the instreamflow requirements and target BOD concentration were also added to the criteria for comparison. As a results, small wastewater treatment plant improved the water cycle due to the increase of low flow and the decrease of BOD concentration. But climate change affected the reduction of effectiveness significantly. Especially in case of construction of small waste water treatment plant in the upstream region, it is necessary to take climate change impact into consideration since it is usually related to the low flow and the water quality of the stream.

• The Journal of the Korea Contents Association
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• v.21 no.4
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• pp.454-462
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• 2021

The purpose of this study was to identify learners with poor learning in basic cooking practice subjects for college students majoring in cooking, operate a basic practical ability improvement program, and apply an action learning teaching method. We tried to analyze the subjective perception of learners using the Q methodology. In addition, it was intended to improve the major competencies for the operation of practical programs related to cooking training, field training, and employment of learners. The survey was conducted from May 1st to 20th, 2020 for first-year students in C cuisine major. As a result of the analysis, a total of three types were derived. Type 1 (N=7): Self-directed learning effect type, Type 2 (N=8): Problem Solving Effect Types, Type 3 (N=6): Peer learning effect type, each unique feature type Was analyzed as. Through the progress of this study and the derivation of implications, it is expected that it will be useful data for the application of teaching and learning methods related to practical work and program operation in cooking-related departments.

• Child Health Nursing Research
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• v.4 no.1
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• pp.116-127
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• 1998

The purpose of this study was to examine the reliability of Q-sorting and to develope a typology of coping to stress in school-age children through the use of Q-methodology. For the study, Q-statements are selected from the literature and interviews of 30 Children, A total number 153 statement were collected and 31 Q-samples were finally selected. AP-sample of 76 was selected the elementary school children. Each of respondents were given 31 coping strategies questionnaire and set of 31 statements with cartoon on each of cards and sorted them according to the 9-point scale. The result of the C-sorting by each subject were coded and analyzed using the QUANAL PC and SPSS PC＋ program. As a result of the analysis, There were significant relationship between score each item of question naire and score of sorted statement. Therefore, school-age children was able to Q-sorting(forced normal distribution). Also, the four types of coping were named as follows : Type I (n=35) : To search for the dynamic behaviors Type II (n=12) : To search for the intellectual behaviors Type III (n=22) : To search for emotional-spiritual behaviors Type IV (n=7) : To search for the self-abuse behaviors Throught the study, the implication for teachers, parents and school nurses is that there is needed to guide and to support adaptive coping behaviors. Also, it is recommended that the application of Q-methodology for study of children's subjective concepts like stress and coping.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.580-586
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• 2000

A soluble Cr(VI) reductase was purified from the Cr(VI) reducing strain Escherichia coli ATCC33456 by ammonium sulfate fractionation, and chromatographies on Q-Sepharose FF, Cibacron blue 3GA dye affinity, Mono-Q 5/5, and Superdex 200 HR 10/30 columns. The estimated molecular mass of the purified enzyme was 27 kDa on SDS-polyacrylamide gel electrophoresis and 54 kDa on gel filtration, thus indicating a dimeric structure. The isoelectric point of the enzyme was pH 4.85. The optimum reaction pH and storage pH were both 7.0, the optimum reaction temperature was $37^{\circ}C$, and the storage temperature was $4^{\circ}C$. NADH and NADPH both served as electron donors for the reductase, with $V_{max}$ of 68.3 ${\mu}M$ Cr(VI)/min/mg protein and Km of 7.6 $\mu$M using HADH, and Vmax of 42.3 ${\mu}M$ Cr(VI)/min/mg protein and Km of 14.6 $\muM$ using NADPH. When 1 mM EDTA was added, the Cr(VI) reducing activity increased 4-fold.

• Honam Mathematical Journal
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• v.41 no.3
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• pp.569-579
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• 2019

Cusp (parabolic) points in the extended modular group ${\bar{\Gamma}}$ are basically the images of infinity under the group elements. This implies that the cusp points of ${\bar{\Gamma}}$ are just rational numbers and the set of cusp points is $Q_{\infty}=Q{\cup}\{{\infty}\}$.The Farey graph F is the graph whose set of vertices is $Q_{\infty}$ and whose edges join each pair of Farey neighbours. Each rational number x has an integer continued fraction expansion (ICF) $x=[b_1,{\cdots},b_n]$. We get a path from ${\infty}$ to x in F as $<{\infty},C_1,{\cdots},C_n>$ for each ICF. In this study, we investigate relationships between Fibonacci numbers, Farey graph, extended modular group and ICF. Also, we give a computer program that computes the geodesics, block forms and matrix represantations.

• Research in Plant Disease
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• v.30 no.3
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• pp.294-299
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• 2024

Polymerase chain reaction (PCR) methods, including conventional PCR (cPCR) and quantitative real-time PCR (qRT-PCR), with both plasmid- and chromosome-targeting primers, are currently the most reliable methods for detecting Erwinia amylovora due to their high sensitivity and specificity. Despite qRT-PCR's quantitative advantage, cPCR remains an attractive method to detect this bacterium in initial screenings of suspected host plants, as it is cost-effective and does not require skilled personnel in well-equipped laboratories. This study aimed to significantly improve cPCR robustness via application of bovine serum albumin (BSA) as a PCR facilitator, with a modified EaF/R primer pair, as previously reported. Experiments have shown that simple supplementation with BSA (10 mg/ml) enhances cPCR reactions using templates such as genomic DNA, bacterial cells, and infected symptomless host organs, including immature apple fruits and seedlings, with EaF/R primers. The cPCR method described in this study is simple, specific, and reliable, and can be applied in routine assays to diagnose fire blight.

• Korean Journal of Environment and Ecology
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• v.28 no.5
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• pp.566-573
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• 2014

Decay rate and nutrient dynamics during leaf litter decomposition of deciduous broad leaf Quercus mongolica and evergreen needle leaf Pinus densiflora were investigated for 69 months from December 2005 to September 2011 in Mt. Worak National Park as a part of National Long-Term Ecological Research Program in Korea. Percent remaining weight of Q. mongolica and P. densiflora leaf litter after 69 months elapsed was $35.4{\pm}2.3%$ and $16.1{\pm}1.3%$, respectively. Decomposition of P. densiflora leaf litter was significantly faster than that of Q. mongolica leaf litter. Decay constant (k) of Q. mongolica and P. densiflora leaf litter after 69 months elapsed was 5.97 and 10.50, respectively. Initial C/N and C/P ratio of Q. mongolica leaf litter was 43.1 and 543.9 respectively. After 69 months elapsed, C/N and C/P ratio of decomposing Q. mongolica leaf litter decreased to 8.7 and 141.2, respectively. Initial C/N and C/P ratio of P. densiflora leaf litter was 151.2 and 391.4, respectively. After 69 months elapsed, C/N and C/P ratio of decomposing P. densiflora leaf litter decreased to 22.9. and 136.5. respectively. Initial concentration of N, P, K, Ca and Mg in leaf litter was 9.30, 0.23, 2.36, 3.14, 1.11 mg/g in Q. mongolica, and 3.02, 0.09, 1.00, 3.84, 0.62 mg/g in P. densiflora, respectively. Initial concentration of N and P in Q. mongolica leaf litter was significantly higher than those in P. densiflora. After 69 months elapsed, remaining N, P, K, Ca and Mg in decomposing leaf litter were 73.8, 60.9, 17.2, 20.3, 35.1 % in Q. mongolica, and 69.5, 75.3, 12.3, 10.9, 10.8 % in P. densiflora, respectively.

• Journal of Climate Change Research
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• v.6 no.4
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• pp.319-330
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• 2015

This study was carried out to predict the optimal growth site and estimate carbon stocks of Quercus acuta, evergreen broad-leaved trees in warm temperate zone according to climate change. The criterion for the optimal site prediction was created by quantification method with quantitative and qualitative data, collected from growth factors of stands and environmental factors of survey sites of 42 plots in Q. acuta by study relationship between growth of tree and site environmental factors. A program for the optimal site prediction was developed by using GIS engine tools. To prediction of the suitable growth site of Quercus acuta, developed program in this study applied to Wando in Jeollanam-do, distributing a various evergreen bread-leaved trees of warm temperate zone. In the results from analysis of the optimal site prediction on Q. acuta, the characteristics of the optimal site showed as follows; site environmental features of class I (the best site class for Q. acuta) was defined as 401 ~ 500 m of altitude, $21{\sim}25^{\circ}$ of slope with above hillside, residual of deposit convex of slope type with west of aspect. The area and carbon stocks of optimal site prediction by class for Q. acuta in classI showed 147.1 ha (2.5%), total 316.5 tC/ha, total $1,161tCO_2/ha/yr$ of class I, 2,703.5 ha (46.3%), total 5,817.4 tC/ha, total $21,331tCO_2/ha/yr$ of class II, 2,845.5 ha (48.6%), total 6,123.0 tC/ha, total $2,845.5tCO_2/ha/yr$ of class III and 153.7 ha (2.6%), total 330.7 tC/ha, total $1,213.7tCO_2/ha/yr$ of class IV.

• Research in Plant Disease
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• v.25 no.2
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• pp.89-93
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• 2019

Fire blight disease caused by the bacterium, Erwinia amylovora, was observed in apple and pear orchards in Korea in 2015. Since then, it has spread, sometimes over long distances to other orchards. Therefore, we examined the ability of E. amylovora to survive in soils and on the surfaces of common materials such as T-shirts, wrist bands, pruning shears, and rubber boots by both conventional PCR (cPCR) and quantitative PCR (qPCR) methods. E. amylovora was detected in all materials tested in this study and survived for sufficiently long periods to cause fire blight disease in new sites. Thus, based on the results of this study, sanitation protocols must be applied to equipment during orchard work.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.648-657
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• 2008

In this study, we used functional genomic strategies, proteomics and quantitative real-time (qRT)-PCR, to advance our understanding of genes associated with fumonisin production in the fungus Fusarium verticillioides. Earlier studies have demonstrated that deletion of the FCC1 gene, which encodes a C-type cyclin, leads to a drastic reduction in fumonisin production and conidiation in the mutant strain (FT536). The premise of our research was that comparative analysis of F. verticillioides wild-type and FT536 proteomes will reveal putative proteins, and ultimately corresponding genes, that are important for fumonisin biosynthesis. We isolated proteins that were significantly upregulated in either the wild type or FT536 via two-dimensional polyacrylamide gel electrophoresis, and subsequently obtained sequences by mass spectrometry. Homologs of identified proteins, e.g., carboxypeptidase, laccase, and nitrogen metabolite repression protein, are known to have functions involved in fungal secondary metabolism and development. We also identified gene sequences corresponding to the selected proteins and investigated their transcriptional profiles via quantitative real-time (qRT)-PCR in order to identify genes that show concomitant expression patterns during fumonisin biosynthesis. These genes can be selected as targets for functional analysis to further verify their roles in $FB_1$ biosynthesis.