• Development and Reproduction
• /
• v.22 no.3
• /
• pp.235-244
• /
• 2018

We investigate the effect of endoplasmic reticulum (ER) stress inhibitor treatment during parthenogenetic activation of oocytes on the ER stress generation, apoptosis, and in vitro development of parthenogenetic porcine embryos. Porcine in vitro matured oocytes were activated by 1) electric stimulus (E) or 2) $E+10{\mu}M$ Ca-ionophore (A23187) treatment (EC). Oocytes were then treated by ER stress inhibitors such as salubrinal (200 nM) and tauroursodeoxychloic acid (TUDCA, $100{\mu}M$) for 3 h prior to in vitro culture. Parthenogenetic embryos were sampled to analyze ER stress and apoptosis at the 1-cell and blastocyst stages. The x-box binding protein 1 (Xbp1) mRNA and ER stress-associated genes were analyzed by RT-PCR or RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. At the 1-cell stage, although no difference was observed in Xbp1 splicing among treatments, BiP transcription level in the E group was significantly reduced by salubrinal treatment, and GRP94 and ATF4 transcription levels in EC group were significantly reduced by all treatments (p<0.05) compared to control. In the EC group, both apoptotic genes were reduced by ER stress inhibitor treatments compared to control (p<0.05) except Caspase-3 gene by TUDCA treatment. These results suggest that the treatment of ER stress inhibitor during parthenogenetic activation can reduce ER stress, and thereby reduce apoptosis and promote in vitro development of porcine parthenogenetic embryos.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.30 no.4
• /
• pp.134-153
• /
• 1988

This paper was carried out to investigate the existence of a unique stress- strain behavior by obtaining some factors influencing the time dependent stress- strain behavior of clay. The results obtained from this study were summarized as follows ; 1. The relationship between stress ratro and strain in normally consolidated clay was in- dependent on pre-shear consolidation pressure. Therefore, shear strain could be expressed as a function with stress ratio. 2. The constitutive equation of shear strain on Modified Carn Clay Model coincided better with the observed value than Cam Clay Model. 3. The relationships between deviator stress and shear strain, between pore water pressure and shear strain were unified by the mean equivalent pressure. 4. The shear strain contour in norrnally consolidated clay was increased linearly through origin, but that in overconsolidated clay was not in accordance with the result of the former. 5. Because the effective stress path of normally consolidated clay was unified by the mean equivalent pressure, state boundary surface in (e,p,q) space was transformed into two dimensional surface. But it was considered to be suitable that the unified stress- strain in overconsolidated clay be expressed by a function with overconsolidation ratio. 6. The deviator for constant strain was increased linearly with increment of strain rate ($\varepsilon$) on semi-log scale, but pore water pressure was decreased. 7. The behavior of stress relaxation was transformed from linear to curvilinear with inc - rement of strain rate before stress relaxation test, and pore water pressure was increased in total range. 8. The strain of creep was increased linearly with increment of time on semi-log scale. The greater the strain rate before creep test became, the greater the increment of strain of creep became. And the pore water pressure during creep test was increased generally with increment of time on semi-log scale.

• The Journal of Korean Academic Society of Nursing Education
• /
• v.14 no.2
• /
• pp.224-231
• /
• 2008

Purpose: The purpose of this study was to analyze the types of the causal perception of preterm labor. Method: The research design was a Q-Methodological Approach. Thirty four selected Q-statements from 32 participants were classified into a shape of normal distribution using a 9 point scale. The collected data was analyzed using a QUANL PC program. Result: Three types of causal perception for research subjects were identified. Type I is negligence of causality; Type II is recognition of causality by preceding factors. Type III is recognition of causality by psychological stress. Conclusion: This study found that there are differences in perceiving causes of preterm labor. Therefore, it is necessary to develop a tailored nursing intervention depending on these types.

• Structural Engineering and Mechanics
• /
• v.18 no.3
• /
• pp.277-286
• /
• 2004

Defective metallic components and structures are being repaired with bonded composite patches to improve overall mechanical and fatigue properties. In this study, fatigue crack growth tests were conducted on pre-cracked 7075/T6 Aluminum substrates with and without bonded Boron/epoxy patches. A considerable increase in the fatigue life and a decrease in the stress intensity factor (SIF) were observed as the number of patch plies increased. The experimental results demonstrate that the patch configurations and patch thickness can enhance fatigue life by order of magnitude. Quantitative comparisons between analytical and experimental data were made, and the analytical model based on a modified Rose's analytical solution appears to best estimate the fatigue life.

• Journal of Environmental Science International
• /
• v.3 no.3
• /
• pp.263-271
• /
• 1994

A detachment of biofilm was investigated in an inverse fluidized bed biofilm reactor(IFRBR). The biofilm thickness, 5 and the bioparticle density, Pm were decreased by the increase of Reynolds number, Re and the decrease of biomass concentration, h. The correlations were expressed as $\delta$=6l.6+16.33$b_c$-0.004Re and Ppd=0.3+0.027$b_c$- 2.93x$l0^{-5}$ no by multiple linear regression analysis method. Specific substrate removal rate, q was derived by F/M ratio and biofilm thickness as q=0.44.+0.82F/M-5.Ix10$-4^{$\delta$}$. Specific biofilm detachment rate, bds was influenced by FIM ratio and Reynolds number as $b_{ds}$=-0.26+0.26F/M+ 2.17$\times$$10^{-4}$Re. Specific biofilm deachment rate in an IFBBR was higher than that in a FBRR(fluidized bed biofilm reactor) because of the friction between air bubble and the bioparticles.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.58 no.3
• /
• pp.496-501
• /
• 2009

Interior Permanent Magnet Synchronous Motor (IPMSM) produces two kind of torque that Magnetic and Reluctance torque. The permanent magnet linkage flux ${\Psi}_a$ and d-axis and q-axis inductances have an important influence on the torque characteristic of IPMSM. Thus their accurate prediction is essential for predicting performance aspect such as the torque and flux-weakening capabilities. In this paper, the influence of barrier width on the ${\Psi}_a$ and $L_d$, $L_q$ is calculated by FEM analysis. Predictions are validated by comparison the average torques, using Maxwell Stress Tensor method.

• ALGAE
• /
• v.39 no.1
• /
• pp.17-30
• /
• 2024

Red macroalga Pyropia yezoensis is a high valuable cultivated marine crop. Its acclimation to cold stress is especially important for long cultivation period across winter in coasts of warm temperate zone in East Asia. In this study, the response of P. yezoensis thalli to low temperature was analyzed on physiology and transcriptome level, to explore its acclimation mechanism to cold stress. The results showed that the practical photosynthesis activity (indicated by Φ_PSII and qP) was depressed and pigment allophycocyanin content was decreased during the cold stress of 48 h. However, the F_v/F_m and non-photochemical quenching increased significantly after 24 h, and the average growth rate of thalli also rebounded from 24 to 48 h, indicating a certain extent of acclimation to cold stress. On transcriptionally, the low temperature promoted the expression of differentially expressed genes (DEGs) related to carbohydrate metabolism and energy metabolism, while genes related to photosynthetic system were depressed. The increased expression of DEGs involved in ribosomal biogenesis and lipid metabolism which could accelerate protein synthesis and enhance the degree of fatty acid unsaturation, might help P. yezoensis thallus cells to cope with cold stress. Further co-expression network analysis revealed differential expression trends along with stress time, and corresponding hub genes play important roles in the systemic acquired acclimation to cold stress. This study provides basic mechanisms of P. yezoensis acclimation to cold temperature and may aid in exploration of functional genes for genetic breeding of economic macroalgae.

• Nutrition Research and Practice
• /
• v.9 no.2
• /
• pp.117-122
• /
• 2015

BACKGROUND/OBJECTIVES: Curcumin, a major component of the Curcuma species, contains antioxidant and anti-inflammatory properties. Although it was found to induce apoptosis in cancer cells, the functional role of curcumin as well as its molecular mechanism in anti-inflammatory response, particularly in intestinal cells, has been less investigated. The intestine epithelial barrier is the first barrier and the most important location for the substrate coming from the lumen of the gut. SUBJECTS/METHODS: We administered curcumin treatment in the human intestinal epithelial cell lines, T84 and Caco-2. We examined endoplasmic reticulum (ER) stress response by thapsigargin, qPCR of XBP1 and BiP, electrophysiology by wild-type cholera toxin in the cells. RESULTS: In this study, we showed that curcumin treatment reduces ER stress and thereby decreases inflammatory response in human intestinal epithelial cells. In addition, curcumin confers protection without damaging the membrane tight junction or actin skeleton change in intestine epithelial cells. Therefore, curcumin treatment protects the gut from bacterial invasion via reduction of ER stress and anti-inflammatory response in intestinal epithelial cells. CONCLUSIONS: Taken together, our data demonstrate the important role of curcumin in protecting the intestine by modulating ER stress and inflammatory response post intoxication.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2000.11a
• /
• pp.561-568
• /
• 2000

본 연구의 목적은 Piezocone 관입시험을 이용한 연약지반의 OCR 평가에 있어 기존의 여러 가지 해석방법들과 최근에 새롭게 제안된 방법들을 실내 모형토조에서 실측된 피에조콘 관입 실험치에 적용하여 각 해석방법들의 차이와 장단점들을 비교 분석하는데 있다. 본 연구의 연구실험방법으로는, Piezocone 관입을 위한 연약 모형지반 조성을 위하여 초대형 Slurry Consolidometer에 Free Stress 상태의 Slurry를 45일간 압밀시킨후 Automatic Computer Control Calibration Chamber (LSU/CALCHAS; Louisiana Slate University Calibration Chamber System)에 옮긴후 다시한번 압밀시키는 Two-Stage Consolidation Method를 사용하였다. 모형지반은 여러 가지 Boundary Condition들과 Stress Condition 그리고 Stress History등을 달리하여 총 5개의 지반을 조성하였다. 관입시험은 총 25개의 Piezocone 관입이 수행되어졌고, 그중 4개는 Standard 10 cm2 Piezocone이고, 나머지 21개는 Miniature Piezocone이 사용되었다. Piezocone 실험치들에 대한 여러 가지 OCR 해석방법 적용결과, Schmertmann방법은 5개 모형지반 모두에서 과다한 OCR평가를 보였으며, $B_{q}$ 방법은 일부모형지반에서 음의 OCR값으로 계산되어졌다. 그러나, Critical-Stale Soil Mechanics 와 Cavity Expansion 이론에 근거하여 Mayne(1991), Kurup(1993), Tumay et al (1995) 들이 제안한 OCR 평가방법들은 실험치와 잘맞는 경향을 보여주었다. 이와같은 이론 모델값들의 차이는 응력조건(Stress Condition)과 경계조건(Boundary Condition)들에 대한 각 해석방법들의 고려정도에 따른 결과로 판단된다.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2005.03a
• /
• pp.916-923
• /
• 2005

In this study, to observe aging effect of undrained shear behavior for Nak-Dong River sand, undrained static and cyclic triaxial tests were performed with changing relative density ($D_r$), consolidation stress ratio($K_c$) and consolidation time. As a result of the test, the modulus of elasticity to all samples estimated within elastic zone by the micro strain of about 0.05% in case of static shear behavior increased with the lapse of consolidation time significantly, so aging effect was shown largely. Also strength of phase transformation point(S_{PT}$) and strength of critical stress ratio point($S_{CSR}$) increased with the lapse of consolidation time. Undrained cyclic shear strength($R_f$) obtained from the failure strain 5% increased in proportion to relative density($D_r$) and initial static shear stress($q_{st}$), $R_f$ of consolidated sample for 1,000 minutes increased about 10.6% compared to that for 10 minutes at the loose sand, and $R_f$ increased about 7.0% at the medium sand. In situ application range of $R_f$ to the magnitude of earthquake for Nak-Dong River sand was proposed by using a increasing rate of $R_f$ as being aging effect shown from this test result.