• 상하수도학회지
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• 제21권5호
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• pp.631-639
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• 2007

This study investigated the construction and operation status of sewer pipes and water-purifier tanks near densely populated areas like large apartment complexes, in order to find out cause for offensive orders. The study results revealed that the main cause arose from the water-purifier tank and public sewer pipes near ordinary residential areas. First, in case of independent water-purifier tanks, the air is forced into the rotten part of large tanks nearby which should be operated in an anaerobic state, so that the tank changes into an aerobic state, or dirty water, which is returned during the sludge return process, falls on the top of the rotten tank, preventing scum from forming within the tank. Such problems cause incompletely purified water in the purifier tank to be discharged, which in turn results in filthy water. Second, in case of public sewer pipes, deteriorated or aging pipes, or the mixture of rain water and dirty water by mixing up combined and separated sewers system can cause foul odors in residential areas. Therefore, offensive odors in residential areas can be radically reduced through the appropriate construction and management of facilities including water-purifier tanks. As well, if more separate sewers are installed as part of an improvement project for public sewer pipes, complaints about foul smell can be minimized.

• 복식문화연구
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• 제12권6호
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• pp.908-917
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• 2004

In the previous article, the effect of aluminum compounds on the mordanting of the Caesalpinia sappan L. dyeing was studied. According to the type of the compound, the effect on the dyeing was found to be different even if $Al^{3+}$ ion was present in the molecular structure of the compound. It was postulated that there would be complex effects other than the effects based on the purified Al compounds only, since there are some amount of compounds hard to neglect. In order to reproduce the traditional dyeing practice, rice straw ash solutions were prepared as mordanting agents. The pH values of the solution baths were 4, 5, 6, 9, and 11 in order to investigate the pH effect on the color development by adding appropriate amount of Schizandra chinensis extract solution. The inherent dyeing properties were evaluated by the analysis of color difference, air permeability, the characteristics developed by pre-mordanting, post-mordanting, or simultaneous mordanting methods.

• Journal of the Korean Wood Science and Technology
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• 제43권5호
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• pp.591-599
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• 2015

Cercidiphyllum japonicum leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. A portion of EtOAc fraction (10 g) was chromatographed on a Sephadex LH-20 column, by the successively elution with various aqueous $MeOH-H_2O$ (1:9, fraction $1-2{\rightarrow}3:7$, fraction $3-5{\rightarrow}1:1$, fraction $6-9{\rightarrow}7:3$, fraction $10-13{\rightarrow}9:1$, fraction 14-16). Compound 2 was isolated from fraction 6 and compound 1 was separated from fraction 11 and 12. Compound 3 and 4 were purified from fraction 13. The isolated compounds were elucidated as quercetin-3-O-${\alpha}$-L-rhamnopyranoside (1), chlorogenic acid (2), quercetin-3-O-${\alpha}$-L-arabinofuranoside (3) and quercetin-3-O-${\beta}$-D-xylopyranoside (4) by the spectral and literature data, and by comparison with the authentic samples. These compounds were reported, for the first time, from the extracts of C. japonicum leaves. Also chlorogenic acid (2) has never been reported before in domestic tree species and can be used as an index compound for C. japonicum.

• Food Science and Biotechnology
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• 제14권5호
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• pp.621-627
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• 2005

Halitosis is mainly caused by the presence of volatile sulfur-containing compounds (VSC's) produced by proteolytic periodontopathic bacteria in the oral cavity. Various mouth-rinses have been offered on the market as solutions to reduce halitosis. The aim of this study was to find a potent substance for the prevention of halitosis. The halitosis prevention substance (HPS) from cumin seed powder was purified by solvent extraction, silica gel column chromatography and preparative TLC to yield an oil phase (0.98%). Instrumental analysis such as FT-IR, $^1H$-NMR and $^{13}C$-NMR showed that HPS contained an -OH group, -HC=CH-, -COO-, and long chain acyl group. HPS was therefore determined to be 2-hydroxyethyl-${\beta}$-undecenate. HPS inhibited the growth of Fusobacterium nucleatum and Porphyromonas gingivalis, by 72.44% and 64.37% at $1{\times}10^{-2}\;M$, and by 99.85% and 91.62% at $5\;{\times}\;10^{-2}\;M$, respectively. It also inhibited the activity of L-methionine-${\alpha}$-deamino-${\gamma}$-mercaptomethane-lyase (METase), which was produced by oral microbes. Furthermore, the VSC production by oral microbes in the human mouth air decreased with increasing HPS concentration. These results suggested that HPS from cumin seed is an efficient halitosis prevention agent.

• Journal of Microbiology
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• 제34권2호
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• pp.206-213
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• 1996

NADH-quinone reductase was purified 22-fold from the cytosolic fraction of Streptomyces sp. Imsnu-1 to apparent hemogenity, with an overall yield of 9%, by the purification procedure consisting of ammonium, sulfate precipitation and DEAE Sephacryl S-200 and DEAE 5 PW chromatographies. Thes molecular mass of the enzyme determined by gel filtration chromatography was found to be 110 kDa. SDS-PAGE revealed that the enzyme consists of two sugunits with a molecular mass of 54 kDa. The enzyme contained 1 mol of FMN per subunit as a cofactor. The $A_{272}$ A$_{457}$ ratio was 6.14 and the molar extinction coefficients were calculated to be 20, 800 and 25, 400M$^{-1}$ $cm^{-1}$ / AT 349 AND 457 nm, respectively. The N-terminal sequence of the enzyme contained the highly conserved fingerprint of ADP-binding domain. The enzyme used NADH as an electron donor and various quinones as electron acceptors. Cytochrome c was practically inactive. Air-stable flavin semiquinone was produced by the addition of NADH to the enzyme. Also, naphthosemiquinone was detected in the reaction mixture containing the enzyme.

• 한국세라믹학회지
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• 제51권4호
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• pp.332-336
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• 2014

A non-electric water purification system using the Korean traditional ceramic ware Onggi, was demonstrated as an appropriate technology to solve water shortages in under developed regions. Generally, Onggi is produced using large size raw materials that are sintered at low temperature, resulting in a porous body that shows air and water permeability. An Onggi filter was prepared using a spinning wheel with the addition of rice bran to the body to increase porosity. The porosity of the obtained Onggi filter was 25.1%; the water permeability was 85.1 $L/m^2h$. Turbidity and TDS of the purified water using Onggi filter were decreased by 97.7% and 29.1%, respectively.

• Archives of Pharmacal Research
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• 제22권2호
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• pp.213-218
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• 1999

The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). The methanol extract and its factions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCl gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH fraction and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and$ 2{\alpha}$-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

• Toxicological Research
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• 제14권4호
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• pp.515-523
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• 1998

Oxidative stress by reactive oxygen species (ROS) damages cellular DNA, RNA, proteins, lipids and others causing various diseases such as cancer, arthritis, and heart diseases. 8-Hydroxyguanine (8-OHG) is one of the products formed from DNA or RNA damaged by ROS. Since high amounts of 8-OHG can be excreted in urine, it may serve as a potential biomarker indicating the level of oxidative damage to nucleic acids. Residents in industrial area with severe air pollution are expected to be affected by higher level of oxidative stress from pollutants like polyaromatic hydrocarbons (PAHs), etc. Smokers are also expected to be damaged by higher level of oxidative stress from cigarette smoke components like PAHs than non-smokers. To examine if the determination of the urinary concentration of 8-OHG could be used as exposure biomarker for the oxidative stress caused by air-pollutants, this study was performed to determine and compare the urinary concentrations of 8-OHG in smokers and non-smokers, or non-polluted area residents and polluted area residents. Urine samples were collected and purified by a strong cation exchange and cellulose partition column, then analyzed by HPLC with electrochemical detector at 600 ㎷ potential. Concentrations of urinary 8-OHG in non-smokers and smokers of Seoul area college male students were determined as 15.12$\pm$9.68 (ng/mg creatinine) and 34.72$\pm$11.72 (ng/mg creatinine), respectively, showing significantly higher level of 8-OHG in smokers than in non-smokers. Urine samples of elementary school students were collected from Sokcho area, which is known to be non-polluted, and 3 representative polluted areas; Yocheon industrial area, Ulsan urban and Ulsan industrial area. The concentrations of 8-OHG in these samples were 12.42$\pm$8.27 (ng/ mg creatinine, Sokcho), 22.55$\pm$9.12 (ng/mg creatinine, Yocheon), 17.41$\pm$2.30 (ng/mg creatinine, Ulsan urban), 55.04$\pm$39.73 (ng/mg creatinine, Ulsan industrial). Thus, samples from polluted area tend to have higher level of 8-OHG and the levels of Yocheon and Ulsan industrial area were significantly higher than that of Sokcho area. The results indicate that the residents of polluted industrial area or smokers are more severely exposed to oxidative stress probably caused by air pollutants like PAHs. Thus, the determination of urinary 8-OHG concentration could be used as biomarker for the extent of body exposure to oxidative stress caused by various pollutants.

• 치위생과학회지
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• 제6권1호
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• pp.47-53
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• 2006

본 연구는 부산광역시 해운대구민의 수불사업에 대한 지식수준을 파악함과 동시에 수불사업 추진 시 나타날 수 있는 문제점에 대한 해결 방안을 마련할 목적으로 2005년 8월 5일부터 20일까지 해운대구민 460명을 대상으로 설문조사를 시행한 결과 다음과 같은 결론을 얻었다. 1. 해운대구민이 섭취하는 식수로서는 정수한 수돗물이 42.8%, 수돗물이 31.3% 였으며, 음식 조리시 사용하는 물의 종류로서 수돗물이 48.3%, 정수한 수돗물이 35.4%로 나타났다. 2. 수불사업에 대해 인지하고 경우는 35.7%, 인지하고 있지않은 경우가 42.0%로 인지하고 있는 경우의 35.7%보다 높게 나타났으며, 모르겠다라고 응답한 경우가 22.4%로 나타났다. 3. 수불사업 목적 인지율은 충치예방이 42.0%로 가장 높았으며, 수돗물 소독이 32.2%, 수돗물 정화가 15.0% 순으로 나타났다. 4. 수불사업 시행 시 찬성여부 및 요인으로는 찬성의 경우가 78.0%로 찬성하는 이유로 51.0%가 충치예방 때문이라고 답했으며, 반대하는 경우는 22.0%로 그 이유를 38.6%가 불소가 인체에 해로워서로 나타났다. 5. 식품과 음료, 물, 공기에 불소가 함유되어 있다는 사실인지여부에서는 인지하고 있는 경우는 21.3%로 낮게 나타났고, 인지하고 있지 않은 경우가 78.7% 였으며, 연령에서는 10대의 93.5%가 식품과 음료, 물, 공기의 불소함유 사실을 모르고 있었다. 6. 수불사업에 대한 안전성 인지율에서는 안전하다고 생각하는 경우는 39.3%였고, 잘 모르겠다는 경우가 55.7%로 가장 높게 나타났으며, 수불사업의 인지획득 경로별 응답률은 언론보도가 28.3%로 가장 높았다.

• 임산에너지
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• 24권2호
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• pp.16-23
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• 2005

삼지구엽초의 각 부분(잎, 줄기, 뿌리)을 MeOH로 추출한 후 농축하고 핵산, 클로로포름, 부탄올, 에틸아세테이트 및 수용성으로 분획하여 동결건조하였다. 각 추출물은 메탄을 수용액 및 에탄올-핵산 혼합액을 사용하여 Sephadex LH-20 칼럼크로마토그래피로 화합물을 분리 한 후 TBA 및 6% 초산에 전개하는 셀룰로오스 박층 크로마토그래피로 단리물질을 확인하였다. 단리물질의 화학적 구조는 $^1H-NMR,\;^{13}C-NMR$ 및 질량 분석으로 결정하였으며 단일화합물로 정제된 물질은 caffeic acid를 비롯하여 flavonoid와 그 배당체 화합물인 icariin, caffeic acid, hyperoside, Ikarisoside A, (+)-catechin이었다. 또한, 각 부분의 클로로포름 분획을 GC/MS (Libraries search)를 이용하여 정성분석을 실시하였다.