• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.314-321
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• 2008

Acaricidal effects of materials derived from Diospyros kaki roots against Dermatophagoides farinae and D. pteronyssinus were assessed using impregnated fabric disk bioassay and compared with that of the commercial benzyl benzoate. The observed responses varied according to dosage and mite species. The $LD_{50}$ values of the chloroform extract of Diospyros kaki roots were 1.66 and $0.96{\mu}g/cm^2$ against D. farinae and D. pteronyssinus. The chloroform extract of Diospyros kaki roots was approximately 15.2 more toxic than benzyl benzoate against D. farinae, and 7.6 times more toxic against D. pteronyssinus. Purification of the biologically active constituent from D. kaki roots was done by using silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by GC-MS, $^1H-NMR,\;^{13}C-NMR,\;^1H-^{13}C$ COSY-NMR, and DEPT-NMR spectra, and identified as plumbagin. The acaricidal activity of plumbagin and its derivatives (naphthazarin, dichlon, 2,3-dibromo-1,4-naphthoquinone, and 2-bromo-1,4-naphthoquinone) was examined. On the basis of $LD_{50}$ values, the most toxic compound against D. farinae was naphthazarin $(0.011{\mu}g/cm^2)$ followed by plumbagin $(0.019{\mu}g/cm^2),$ 2-bromo-1,4-naphthoquinone $(0.079{\mu}g/cm^2)$, dichlon $(0.422{\mu}g/cm^2)$, and benzyl benzoate $(9.14{\mu}g/cm^2)$. Additionally, the skin color of the dust mites was changed from colorless-transparent to dark brown-black by the treatment of plumbagin. Similar results have been exhibited in its derivatives (naphthazarin, dichlon, and 2-bromo-1,4-naphthoquinone). In contrast, little or no discoloration was observed for benzyl benzoate. From this point of view, plumbagin and its derivatives can be very useful for the potential control agents, lead compounds, and indicator of house dust mites.

• The Plant Pathology Journal
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• 제33권3호
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• pp.318-328
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• 2017

Chitinase-producing Paenibacillus elgii strain HOA73 has been used to control plant diseases. However, the antimicrobial activity of its extracellular chitinase has not been fully elucidated. The major extracellular chitinase gene (PeChi68) from strain HOA73 was cloned and expressed in Escherichia coli in this study. This gene had an open reading frame of 2,028 bp, encoding a protein of 675 amino acid residues containing a secretion signal peptide, a chitin-binding domain, two fibronectin type III domains, and a catalytic hydrolase domain. The chitinase (PeChi68) purified from recombinant E. coli exhibited a molecular mass of approximately 68 kDa on SDS-PAGE. Biochemical analysis indicated that optimum temperature for the actitvity of purified chitinase was $50^{\circ}C$. However, it was inactivated with time when it was incubated at $40^{\circ}C$ and $50^{\circ}C$. Its optimum activity was found at pH 7, although its activity was stable when incubated between pH 3 and pH 11. Heavy metals inhibited this chitinase. This purified chitinase completely inhibited spore germination of two Cladosporium isolates and partially inhibited germination of Botrytis cinerea spores. However, it had no effect on the spores of a Colletotricum isolate. These results indicate that the extracellular chitinase produced by P. elgii HOA73 might have function in limiting spore germination of certain fungal pathogens.

• 환경영향평가
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• 제7권2호
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• pp.53-64
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• 1998

This study was performed to build up the ecological guidelines to grasp the structure of the vegetation change which is due to river rehabilitation. Anyway, river ecosystem and function has been destroyed owing to river development. It is important that river vegetation supplies ecological corridor and biotope. Two survey sites(Wonsungcheon and Pungseocheon)were investigated in the aspect of plant ecosystem and structure to settle the practical concept of river ecosystem. Each survey site was subdivided to five plots. The site was surveyed through the belttransect method. Wonsungcheon gets more seriously polluted as it runs to the urban area. In other words, there are On the other hand, Pungseocheon has more naturality but its downstream is under the pressure of various wood plants in the upstream area, but downstream area is dominated by naturalized plants such as Bidens frondosa, Panicum dichotomiflorum, etc. Riverbank of downstream has been changed into farm and parking lot. development. It should be preserved definitely because it still has abundant naturality and wetland which formed a biotope. The objective of the research is to find out the river retrogression and maintenance methods based on the riparian vegetation structure. To manage the river ecologically, hydrophytes should be induced partly for natural purification after the riverside is rehabilitated. The vegetation should be induced step by step to restore natural river and steady monitoring and research are required.

• 농촌계획
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• 제24권2호
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• pp.47-58
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• 2018

Protected horticultural complexes would increase crop productivity but would adversely affect the groundwater recharge function in the area because the impervious area would increase. Further, they would limit the movement of living beings, affecting biodiversity. Therefore, this study evaluated the groundwater ecosystem services provided by protected horticultural complexes in terms of consistent utilization of water. The estimated amounts of groundwater loss obtained through quantitative assessment of groundwater infiltration showed that a higher impervious area results in higher losses. We, therefore, predict a much higher loss if similar changes in land use are realized on a nationwide scale. A plan to promote groundwater recharge in impervious areas is actively being discussed for urban areas; however, this plan is not yet applicable to farming areas. We consider it is essential to develop groundwater infiltration facilities for horticultural complexes, infiltration trenches, permeable pavements, surface water storage facilities, water purification facilities, etc. Further research and development of groundwater infiltration facilities is important for consistent utilization of water and the improvement of ecosystem services.

• 자원리싸이클링
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• 제14권5호
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• pp.54-61
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• 2005

국내 경제성장과 더불어 발전소 에너지원으로 이용되고 있는 석탄은 매년 증가하여 2006년에는 전체 에너지 구성의 30%인 16,000MW에 이를 것으로 예측되고 있다. 이러한 석탄은 다른 화석연료에 비해 매장량이 풍부하고 경제성은 높지만, 사용이 불편하고 석탄회, 아황산가스 등 많은 공해물질을 배출하고 있다. 따라서 본 연구에서는 마찰하전형정전선별법을 이용하여 석탄과 회분구성 광물의 선별기술을 확립, 청정석탄을 생산하고자 한다. 본 연구에서는 bench-scale의 마찰하전형정전선별 장치를 제작하였으며, 선별에 영향을 미치는 인자들을 선정하고 분리실험을 수행하여 최적조건을 확립하였다. 연구결과 최적조건에서 석탄 회수율과 회분 및 유황분 제거율이 각각 68.10%. 31.23% 그리고 28.33%인 정제석탄을 얻을 수 있었다.

• 한국조경학회지
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• 제31권3호
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• pp.83-90
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• 2003

This study explored effects of urban greenspace on improving atmospheric environment, which is concerned with $CO_2$, SO$_2$ and NO$_2$ uptake, and with reduction of summer air temperatures. The site of this study was focused on Jung-gu in Seoul. Tree density and cover were 1.1 trees/100 $m^2$ and 12.5% respectively for the study area except forest lands. Atmospheric purification by greenspace was associated with changes in tree cover per unit area of each land use type. The mean $CO_2$ storage by woody plants was 19.4t/ha, and annual uptake averaged 2.2t/ha/yr for $CO_2$, 1.9kg/ha/yr for SO$_2$ and 5.0kg/ha/yr for NO$_2$. Entire tree plantings in the study area played a significant role by annually offsetting $CO_2$ emissions of about 1,830t from fossil fuel consumption by 330 persons, SO$_2$ emissions of 1,620kg by 1,080 persons, and NO$_2$ emissions of 4,230kg by 450 persons. The summer air temperature was 3.6$^{\circ}C$ cooler at a location with 54% cover of woody plants and 4.5$^{\circ}C$ cooler at a forest site with 100% cover, compared to a place with no planting. A 10% increase of woody plant cover was estimated to decrease summer air temperature by approximately 0.6$^{\circ}C$ until a certain level of canopy cover. Analyzing data from the Automatic Weather Stations in Seoul revealed that increasing tree cover decreased mean air temperature for the summer season (Jun~Aug) in a nonlinear function. Woody plant cover was the best predictive variable of summer temperature reduction. The results from this study are expected to be useful in emphasizing the environmental benefits and importance of urban greenspace enlargement, and in urging the necessity for planting and management budgets.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2016년도 추계학술대회
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• pp.274-276
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• 2016

현재 기호 식품으로 여겨지는 담배 사용자의 증가로 인해 많은 문제점으로 화두가 되고 있다. 공공장소에서의 흡연뿐만 아니라 실내 흡연 등으로 비흡연자들에게 피해를 주고 있는 실정이다. 흡연부스가 설치되어 있다고 하지만 그 수량이 현저히 적고, 흡연부스 내 정화장치가 제대로 작동하지 않은 경우가 많아 흡연자들이 흡연부스 밖에서 흡연을 하여 주변 사람들에게 피해를 주고 있다. 본 논문에서는 이러한 흡연자들에게 금연경고 이미지와 건강경고 메시지를 효과적으로 인식할 수 있도록 하게 하기 위해, Kinect Camera Sensor와 증강현실(Augmented Reality: AR) 기능을 이용하여 사람의 모션을 좌표값으로 인식하고 해당 좌표값이 가까워지면, 금연 광고판이 실행되는 '증강현실 금연 광고판'을 소개한다.

• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1310-1315
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• 2019

Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and assess its function. Therefore, in order to find the most efficient active hyaluronidase, we produced several shortened hyaluronidases with partial removal of the N- or C-terminal regions. Moreover, we created an enzyme that connected six histidines onto the end of the hyaluronidase C-terminus. This simplified subsequent purification using $Ni^{2+}$ affinity chromatography, making it feasible to industrialize this highly active recombinant hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme. Therefore, this simple and effective isolation method could increase the availability of recombinant hyaluronidase for research and clinical purposes.

• 농업과학연구
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• 제48권4호
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• pp.935-944
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• 2021

It has been suggested that bisphenol A (BPA), a known endocrine disruptor, interferes with the endocrine system, causing reproductive dysfunction. Recently, BPA has been found in waste water due to incomplete sewage purification, possibly threatening health through its ingestion via tap water. In this study, young male mice (6 - 7 weeks old) were administered water containing BPA (50 mg·kg^-1) for four weeks, while control mice consumed water without BPA. Serum, epididymal spermatozoa and testicular sections were assessed after sacrificing the mice on day 28. No significant differences were obtained between the groups in the body, testis and seminal vesicle weights. However, the epididymal sperm motility and count levels were significantly reduced in BPA-fed mice. Significantly higher hepatotoxicity levels were also observed in mice ingesting BPA as compared to the control mice. The level of serum testosterone was reduced, and testicular sections revealed incomplete and irregular spermatogenesis in BPA-ingested mice. The sperm proteasomal-proteolytic activity level has been implicated in sperm function and is measured in motile spermatozoa using fluorometric substrates. High ubiquitin C-terminal hydrolase activity levels were observed in the control mice without BPA. During a mating trial, a low pregnancy rate (71.4%) was observed in females mated with males who had consumed BPA (100% in the control mice). Overall, BPA adversely affected spermatogenesis and quality, as indicated by decreased sperm motility, concentration and serum testosterone levels, resulting in reduced fertility competence.

• 인간식물환경학회지
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• 제22권5호
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• pp.411-424
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• 2019

This study analyzed the factors affecting urban residents' expectations and participation in community gardens to present basic data of guidelines for community garden management. Urban citizens valued the necessity of community gardens overall, especially residents with outdoor gardens. The experience of private gardens was important in perceiving the need for community gardens, which are public spaces. The creation of community gardens had high expectations for social aspects such as improving quality of life, health promotion, and community revitalization. In particular, residents living in individual housing have high expectations for revitalization of the community, and community gardens can be a mediator for forming and maintaining local communities. Through this, the citizens' perception on the role and function of the garden as a community space could be examined. Expectations for quality of life, health promotion, and community revitalization are factors affecting the necessity of community garden. Participation was high in the creation and management of the community garden, and the necessity of community gardens, expectation for community revitalization, and expectation for environmental purification are factors that affect participation in the management of community gardens. The amount of donations that the residents are willing to pay for community garden management was KRW 75,222 /year on average. Overall, residents with higher need for community gardens and higher social expectation showed higher participation. Urban citizens have high expectations for community gardens, and they perceive the importance of social aspects more than personal aspects such as community revitalization and quality of life. As a strategy for increasing community involvement, it is necessary to set up the processes, determine participants' composition and roles, and establish a system to promote participation. Considering welfare aspects such as community revitalization and improvement of residents' quality of life, motivation and administrative support for participation in the creation and management of community gardens will serve as important factors.