• Title/Summary/Keyword: Pure culture

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Highly Pure Culture of Photosynthetic Bacteria for Pilot-scale Production (광합성세균의 파이롯트 단위 생산을 위한 고순도 배양)

  • Cho, Kyoung Sook;Yim, Tae Bin;Jeong, Hae Yoon;Cho, Jeong Sub;Kim, Joong Kyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.4
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    • pp.292-297
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    • 2006
  • Lab-scale and pilot-scale productions of photosynthetic bacteria, that were able to efficiently treat wastewater from aquacultural farm, were experimented for their highly-pure culture. The results of experiments in a gas pack reactor, an anaerobic flask and a flask using $N_2$ gas as N-source showed that only photosynthetic bacteria formed red colonies on agar plate and their purity was over 90% in a colony, observed under a microscope. It was found that the basal medium could most promote the growth of photosynthetic bacteria, confirmed by experiments of serial cultures on various media. Under the culture conditions, the specific growth rate was found to be $0.18h^{-1}$ from the culture in 5L bioreactor and the same value could be obtained in pilot-scale production.

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Improvement of Plants by Biotechnology (세포공학을 이용한 식물개량)

  • 윤의수
    • Korean Journal of Plant Resources
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    • v.3 no.1
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    • pp.1-30
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    • 1990
  • The traditional plant imprownent methods consisted of pure line selection, cross breeding, heterosis breeding, polyploid breeding, mutati-onbreeding, ect.Biotechmoiogy is divided into gene spliclng , monocle-nal antibodies , protein engineering , agricultural research, and microbiological engineering. Of these , high plants deal with agricultural research, and the importent part of which is tissue culture and celLculture , Tissue .culture and cell culture are again divided into embryoculture, test tube fertilization, anther and pollen culture, somatichybridization , transformation, recombination, recombinant DNA moleculehybrid plasmid, ect For these haploid production, protoplast culture,protoplast fusion, selection and propagation, ect. , the technical sett-lement is needed.

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Effects of Combined Micronutrient(Fe, Mn, Cu, Zn, Mo and B) Application on Forage Traits in Pure and Mixed Swards of Orchardgrass and White Clover III. Changes in the contents and yields of N-compounds(crude/pure protein and soluble N-compounds) in forage plants (Orchardgrass 및 White Clover의 단파 및 혼파 재배에서 미량요소(Fe, Mn, Cu, Zn, Mo, B)의 조합시비가 목초의 특성에 미치는 영향 III. 목초 중 질소화합물(조/순단백질 및 수용성 질소화합물)의 함량 및 수량 변화)

  • 정연규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.1
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    • pp.1-10
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    • 2004
  • This pot experiment was conducted to investigate the effects of combined micronutrient application($T_1$;control, $T_2$; Fe, $T_3$; Fe+Mn, $T_4$; Fe+Mn+Cu, $T_5$ ; Fe+Mn+Cu+Zn, $T_6$ ; Fe+Mn+Cu+Zn+Mo, $T_7$ ; Fe+Mn+Cu+Zn+Mo+B) on forage performance of pure and mixed cultures of orchardgrass and white clover. The third part was concerned with the changes in the contents and yields of N-compounds (crude/pure protein and soluble N-compounds) in forages. The results obtained are summarized as follows: 1. The contents of N-compounds(crude/pure protein and soluble N-compounds) were generally different according to the forage species, whether it was a pure or mixed culture, and additional fertilization, especially N. In orchardgrass, these contents were relatively low at the $T_3$ and $T_6$ in both pure and mixed cultures. In white clover, these contents were relatively decreased by the $T_1$, $T_3$, and $T_6$ in mixed culture. 2. The treatments influenced relatively more on the yields of crude/pure protein than on the dry matter yields of forages, and this tendency was more significant in white clover than in orchardgrass. 3. In white clover, the great differences in the yields of crude protein by the treatments occurred especially in mixed culture and at 5th cut without no additional fertilization. In white clover, the positive effects of optimum treatments on the yields of crude protein seemed to be decreased by the additional fertilization, especially N. In mixed culture, the favorable growth of white clover by the optimum treatments tended to be positively related to the favorable contents and yields of N-compounds. The changes in the yields of pure protein were similar to the tendency of crude protein

Biocides Effect on the Microbiologically Influenced Corrosion of Pure Copper by Desulfovibrio sp.

  • Onan, Mert;Ilhan-Sungur, Esra;Gungor, Nihal Dogruoz;Cansever, Nurhan
    • Journal of Electrochemical Science and Technology
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    • v.9 no.1
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    • pp.44-50
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    • 2018
  • The aims of this study were to determine the corrosion behavior of pure copper in the presence of Desulfovibrio sp. and also to investigate the effects of glutaraldehyde (GD) and isothiazolinone (ISO) on the corrosion behavior of pure copper in the presence of this sulfate-reducing bacteria (SRB) strain by using electrochemical techniques. Electrochemical measurements of pure copper were carried out at specified time intervals (0, 8, 24, 48, and 96 hr) over a period of exposure. Corrosion rates of pure copper from anodic and cathodic Tafel slopes and corrosion potential ($E_{corr}$) were determined. Biofilm and corrosion products on the copper surfaces were observed by Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-Ray Spectrometry (EDS) analyses. The effects of solution types (PC (Postgate's C medium) and SRB (Desulfovibrio sp.)) and exposure times of copper and biocides (ISO or GD) on the corrosion rates of pure copper were evaluated by statistical analyses. As a result of the FESEM analysis, biofilm formation was observed on the surfaces of pure copper exposed to the Desulfovibrio sp. cultures both with and without the biocides. The results show that the pure copper was corroded by Desulfovibrio sp. However, the addition of GD or ISO to the Desulfovibrio sp. culture resulted in a decrease in the corrosion rate of the pure copper. It was also observed that both of the biocides showed a similar effect on pure copper's corrosion rate caused by Desulfovibrio sp.

Alcaligenes eutrophus 균주의 성장과 Poly-Beta-hydroxybutyrate 합성에 미치는 포도당과 암모늄농도의 영향

  • 이용우;유영제
    • Microbiology and Biotechnology Letters
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    • v.18 no.6
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    • pp.607-612
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    • 1990
  • The biodegradation of Aroclor 1242 was investigated by the mixed cultivation of the natural bacterial isolates and a genetically engineered microorganism (GEM). The natural strain of MS-1003 degraded the Aroclor 1242 through the ortho-cleavage pathway, while the other strains through the meta-cleavage pathway. When the MS-1003 strain was additionally inoculated into the 1 day culture of the DJ-26 strain and then cultivated for 2 days, the Aroclor was degraded up to 86% and resulted in increase of the meta-cleavage product. But in the MS-1003 culture inoculated with the DJ-26, degradation of the Aroclor was limited to the level of each pure culture. By the mixed cultivation of the DJ-26 strain together with the DJ-12 or its GEM strain of DF-10, which degrades the Aroclor through the meta-cleavage pathway, degradation of the Aroclor as well as production of the meta-cleavage compound were lower than those of each pure culture. The degradation of Aroclor 1242 by the GEM strain was not improved over the parental strain. Therefore, a form of cometaboiism of Aroclor 1242 was found in the mixed culture of the DJ-26 and MS-1003 strains which degrade the Aroclor through the different metabolic pathway, but in the mixed culture of the DJ-26 and DJ-12 strains degrading Aroclor 1242 through the same pathway, a kind of competetion for the substrate was observed.

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복식에 표현된 드래퍼리에 관한 연구

  • 이은영
    • The Research Journal of the Costume Culture
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    • v.7 no.1
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    • pp.52-67
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    • 1999
  • A Study on the Drapery Presented in Costume The purpose of this study is to find esthetic characteristic of a drapery costume throughout Greece costume and modern fashion. For this purpose, documentary studies were proceed. And photos of the drapery costume were analyzed. Drape is the hang or fall of fabric when made into a garment is an important quality to consider indesigning. Fabric falling in folds in the garment as seen on statues of ancient Greece ; most outstanding modern versions made by designer Gres and Vionnet. Greek costume was in drapery types mostly and a feature created the vest esthetic effert on simplicity with ornament of drapery. Grecian were expressed pure beauty and natural beauty throughout their drapery costume. Madame Gres is widely considered one of the most talented greece for inspiration of the couture, ranked by many with Vionnet. They shows in their mastery of Greek draping -el-egance and simplicity- And also, they always implicity emphasized the relationship between clothing and the female body. Therefore drapery costume admired beauty of pure human body Clothing is expressed which wanted to get the nature and human, search for the losed nature of modern men. In conclusion, the characteristic of drapery costume from ancient greece to modern, timeless concerned human\`s lives and recurring humanity.

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The Problem of Pure Language in Walter Benjamin's "The Task of the Translator" from the Perspectives of Paul De Man, Gilles Deleuze, and Jorge Luis Borges (벤야민의 「번역가의 과제」와 폴 드만, 들뢰즈, 보르헤스)

  • Kim, Jiyoung
    • Cross-Cultural Studies
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    • v.33
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    • pp.309-330
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    • 2013
  • This paper explores the concept of pure language introduced in Walter Benjamin's "The Task of the Translator" and looks at various perspectives on this concept represented in theories of Paul De Man and Gilles Deleuze and a short story of Jorge Luis Borges. According to "The Task of the Translator," pure language is defined as a vessel of which fragments are the original and the translation. Just as fragments are part of a vessel, so the original and the translation are fragments of a greater language, which is pure language. On the other hand, De Man, from a deconstructive criticism, says that pure language does not exist except as a permanent disjunction, which inhabits all languages as such, and that any work is totally fragmented in relation to this pure language and every translation is totally fragmented in relation to the original. While De Man consider pure language incorporeal, Deleuzian interpretation regards it as a virtual object or differenciator in relation to which the two series of the original and the translation coexist and resonate. Finally in Borges's "Pierre Menard, Author of the Quixote" Menard attempt to translate Cervantes's Don Quixote identically in every detail. By showing a case in which the original and the translation are the same, Borges raises a question what would take place in relation to pure language if the original and the translation were identical. In Deleuze, identity and resemblance are the result of a differenciator, but in Borges, identity is a differenciator which produces differences. If we apply this logic to the last paragraph of "The Task of the Translator," we can say the interlinear version of Scriptures, as the prototype or ideal of all translation, in the form of which the original and the translation must be one, is a differenciator, an endless difference-making machine.

Comparative Analysis of Bacterial Diversity in the Intestinal Tract of Earthworm (Eisenia fetida) using DGGE and Pyrosequencing (DGGE 방법과 Pyrosequencing 방법을 이용한 지렁이 장내미생물의 다양성 분석)

  • Kim, Eun-Sung;Hong, Sung-Wook;Chung, Kun-Sub
    • Microbiology and Biotechnology Letters
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    • v.39 no.4
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    • pp.374-381
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    • 2011
  • The beneficial effects of Eisenia fetida on soil properties have been attributed to their interaction with soil microorganisms. The bacterial diversity of the intestinal tract of E. fetida was investigated by culture-dependent and culture-independent methods including denaturing gradient gel electrophoresis (DGGE) and pyrosequencing analyses. In a pure culture, Lysinibacillus fusiformis (51%), Bacillus cereus (30%), Enterobacter aerogenes (21%), and L. sphaericus (15%) were identified as the dominant microorganisms. In the DGGE analyses, B. cereus (15.1%), Enterobacter sp. (13.6%), an uncultured bacterium (13.1%), and B. stearothermophilus (7.8%) were identified as the dominant microorganisms. In the pyrosequencing analyses, Microbacterium soli (26%), B. cereus (10%), M. esteraromaticum (6%), and Frigoribacterium sp. (6%) were identified as the dominant microorganisms. The other strains identified were Aeromonas sp., Pseudomonas sp., Borrelia sp., Cellulosimicrobium sp., Klebsiella sp., and Leifsonia sp. The results illustrate that culture independent methods are better able to detect unculturable microorganisms and a wider range of species, as opposed to isolation by culture dependent methods.

Oxidative Stability of Hydrated Soybean Oil during Heating at High Temperature (고온가열 과정 중 대두경화유의 산화안전성에 관한 연구)

  • Kim, Myong-Ae
    • Journal of the Korean Society of Food Culture
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    • v.23 no.1
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    • pp.26-32
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    • 2008
  • : In this experiment, three samples of oils were used. These oils were hydrated soybean oil, pure soybean oil and regular soybean oil. Oil was used after heating at $235-240^{\circ}C$ every four hours term and total heating hours was 16 hours. The physio-chemical analysis and sensory evaluation were performed on these oils. The hydrated soybean oil showed lower acid, peroxide and carbonyl value than the other two oils (p<0.05 or p<0.001). The other two oils were more affected in rancidity than the hydrated soybean oil. In color test, whereas L value lowed during the heating time, a and b value increased during the heating time. The tendency of high L value and low b value in a long-time heating was more apparant on the pure soybean oil and the regular soybean oil than on the hydrated soybean oil (p<0.05). In sensory evaluation, color and rancidity order increased during the heating time. The hydrated soybean oil showed color and rancidity order than the other two oils. In overall quality test, the pure and regular soybean oil that had been used for 12 and 16 hour were not preferable. The hydrated soybean oil that had been used for 16 hour were not preferable. In sensory evaluation, the hydrated soybean oil, the pure and regular soybean oil did not show a apparent difference, although the hydrated soybean oil had a little better scores on the overall quality.

Development of a Sound Art Programming Course for Non-Majors (비전공자를 위한 사운드 아트 프로그래밍 교과목 개발)

  • Kwon Hyunwoo
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.4
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    • pp.71-79
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    • 2024
  • This study developed a sound art programming course using pure data to foster computational thinking and convergence of art and technology in college students who are non-computer majors. This paper presents an example of operating a curriculum that designed and developed a sound art-centered music programming subject using Pure Data, derives educational outcomes and improvement measures for classes, and presents a creative convergence education program of technology and art. It has a purpose. For the study, we looked at examples of educational programs that combine art and technology, as well as pure data and sound art, and based on this, we designed and developed a sound art programming course for non-majors. The curriculum was operated based on the developed subjects, and the results showed increased interest in programming through art and technology convergence classes, active class participation through autonomous choice, creation of a new perspective on art, improvement of computational thinking skills, collaboration and communication. The educational effect of ability enhancement was confirmed. We expect that this study will be able to present a new perspective on the convergence education of art and technology, including artistic diversity and understanding of new media according to the development of media.