• 대한한의진단학회지
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• 제10권2호
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• pp.104-120
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• 2006

Background : Pulse-respiration ratio has been used for estimating subject's status in oriental medicine. Pulse and respiration is strongly associated with autonomic nerve system. But there is no research about correlation between pulse-respiration ratio and autonomic nerve system. Objectives : We performed this study to know correlation between pulse-respiration ratio and HRV(heart rate variability) that shows autonomic nerve system status well and to clarify clinical meaning of pulse-respiration ratio. Methods : After subject's 10 minutes rest, we measured subject's ECG, respiration pattern and HRV. In this research, subject's number is 95(Male 50/Female 45). We calculated pulse-respiration ratio from ECG and respiration pattern. Then, we analyzed correlation between pulse-respiration ratio and HRV parameters in all subjects, 2 group divided by Wan-Maek(P-R ratio 4.28). We tried to compare HRV parameters among Wan-Maek, Sak-Maek and Ji-Maek group. Correlation analysis between pulse-respiration Ratio and Pulse rate, respiration rate is performed. Finally correlation analysis between Respiration and HRV parameters in all subjects, 2 group divided by Wan-Maek(4.28) is studied. Results : 1. Mean pulse-respiration is 4.10${\pm}$0.67, Mean pulse rate is 68.06${\pm}$7.82bpm, Mean respiration rate is 16.81${\pm}$2.72 times per minute in all subjects. 2. Correlation analysis between pulse-respiration ratio and HRV parameters of high pulse-respiration ratio group is not significant. But, in low pulse-respiration ratio group, HFnorm(correlation coefficient 0.306, p= 0.018), lnHF (0.308, p=0.002) is significantly correlated with pulse-respiration ratio. 3. Comparison of HRV parameters among Wan-Maek, Sak-Maek and Ji-Maek Group is not significant. 4. Pulse-respiration ratio is more affected by respiration rate(correlation coefficient-0.17, p=0.000) than pulse rate (correlation coefficient 0.396, p=0.000). 5. Correlation analysis between respiration rate and HRV parameters of high pulse-respiration ratio group is not significant. But, in low pulse-respiration ratio group, HFnorm (correlation coefficient -0.327, p=0.011), LF/HF(0.346, p=0.007), lnHF (-0.355, p=0.006) are significantly correlated with respiration rate. Conclusion : Pulse-respiration ratio and parasympathetic index has positive correlation. The closer Wan-Maek, The higher parasympathetic index in low pulse-respiration ratio group. Respiration rate is more related with pulse-respiration ratio than pulse rate. Respiration is negatively correlated with autonomic parameters. And the slower respiration, the higher parasympathetic index in low pulse-respiration ratio group.

• 대한한의학회지
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• 제18권1호
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• pp.143-156
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• 1997

This report was conducted to quantify the pulse/respiration ratio and set up the normal range of wan-maeck(緩脈). In order to objectify the pulse diagnosis and use as basic clinical index of Cold-Hot diagnosis, we developed the hardware and software for detection and interpretation of pulse/respiration ratio, pulse/expiration ratio, pulse/respiration ratio, inspiration time, expiration time, respiration frequency, respiration time, duration of one pulse and pulse and pulse rate per minute, The results were as follows; pulse/respiration ratio is $4.30{\pm}1.03$ times, pulse/respiration ratio is $1.60{\pm}0.32$ times, pulse/respiration ratio is $2.37{\pm}0.75$ times, inspiration time is $1.35{\pm}0.20$ sec, expiration time is $1.89{\pm}0.39$ sec, respiration frequency is $17.16{\pm}3.49$ times/min, total respiration time is $3.63{\pm}0.71$ sec, duration of a pulse is $0.86{\pm}0.15$ sec, pulse rate is $71.51{\pm}12.30$ times/min.

• 대한한의진단학회지
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• 제9권2호
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• pp.123-144
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• 2005

Objectives: Pulse-Respiration Ratio has been used for estimating subject's Han-Yeol [寒熱] status since it mentioned in suwen [素問]. In practicing Pulse-Respiration Ratio over 5 means the status of Yeol [熱], Pulse-Respiration Ratio below 3 means the status of Han [寒]. We performed this study to examine the Optimum Standard for Measuring Pulse-Respiration Ratio on the Basis of Repeatability and Reproducibility. Methods: After subject's 5 minutes rest we measured subject's ECG, respiration pattern, EEG, EMG simultaneously. In this research examiner's number is two, subject's number is four, and the number of repeat is two. We calculated Pulse-Respiration Ratio through dividing Respiration cycle average by Pulse cycle average according to each standard including time section, $EEG(relative-{\alpha}$ density, $relative-{\beta}$ density, ${\alpha}/{\beta}$ and EMG. We analyzed these data through Gage R&R study using MINITAB 13.20 program and considered the results of below 30 %R&R and over 4 Number of Distinct Categories to have a significance. Results: 1. In the applying of time standard, Pulse-Respiration Ratio from section 3, 4, 6, 8 had a significant meaning in the aspect of Repeatability and Reproducibility. 2. In the applying of $EEG({\alpha}$ I , ${\beta}$ I , ${\alpha}/{\beta})$, EMG(E I) standard, there was no significant results. 3. In the applying of time standard(section 5, 6, 7), $EEG({\alpha}$ I , ${\beta}$ I , ${\alpha}/{\beta})$ and EMG(E I) standard simultaneously, Pulse-Respiration Ratio from ${\alpha}/{\beta}$ in section 6, ${\beta}$ I in section 8 had a significant meaning in the aspect of Repeatability and Reproducibility. Conclusions: We can suggest the Optimum Standard for Measuring Pulse-Respiration Ratio on the basis of Repeatability and Reproducibility as followings; 1. Pulse-Respiration Ratio Measuring time should be at least 15 minutes. 2. Applying of time(section 6, 8) and $EEG({\beta}$ I, ${\alpha}/{\beta})$ standard simultaneously is recommended considering reliability and validity but more study is needed. 3. EMG(E I) may be helpful to detect the segment of physical rest and exclude artifacts but more study is needed.

• 동의생리병리학회지
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• 제28권6호
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• pp.668-673
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• 2014

Cold and Heat pattern identification(CHPI) in traditional East Asian medicine(TEAM) is one of the major indicator to distinguish characteristics of disease and to determine treatment method. Basic parameters to determine CHPI include the pulse rate, respiration rate, and pulse power. Studies to associate physiological responses of human body by cold stress(CS) with CHPI in TEAM were rarely done so far. This study aims to explore the effects of cold stress on pulse signal via a feasibility study for three subjects and investigate some indices which can reflect autonomic nerve reaction(ANR). We measured radial pulse signals and respiration signal of the investigated subjects before the CS, during the CS which continues for 5 minutes, and immediately after the CS, respectively. Finally, we analyzed the pulse rate (P), respiration rate (R), pulse power, pulse depth, and pulse rate per respiration (P/R ratio). As a result, the P/R ratio showed a consistently decreasing tendency through the CS stimulation process, while other parameters behaved more complex and in subject-specific ways. It implies that, among candidate parameters, the P/R ratio is a simple but the most probable parameter that can be used as the ANR indicator. This result is also consistent with the theory in TEAM scripts, in which the P/R ratio is predicted to be a direct indicator for the CHPI. This pilot study shows that P/R ratio can be more appropriately associated with the ANR than heart rate or respiration rate alone. Extensive studies will be necessary to verify or confirm the P/R ratio as an appropriate and well defined parameter for ANR.

• 대한한의진단학회지
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• 제10권2호
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• pp.132-150
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• 2006

Objectives : The objective is that we measure and analyze Pulse-Respiration Ratio and Body Composition Analysis to study the correlation between both. Methods : First, after subjects take a rest over 10 minutes, we measure their electrocardiogram and respiration pattern through which we take average peak interval to calculate an average pulse cycle and a respiration cycle. An average respiration cycle divided by an average Pulse Rate gives Pulse-Respiration Ratio. Next, we draw out 22 Body Composition Analysis indicators by using In-Body 720 model. Last, we analyze and take statistics on them by using SPSS 13.0 program. Results : Negative is the correlation between P/R Ratio and Body Composition Analysis indicator like fatness degree, body fat volume, body fat rate, abdominal fatness, BMI. Conclusions : 1. The higher P/R Ratio the more likely to be thin, the lower P/R Ratio the more likely to be fat. 2. We separately analyze P/R Ratio depending on each breathing frequency and pulse frequency to find out that breathing frequency has great influence and that breathing frequency decides the fatness degree. 3. In study on the correlation between P/R Ratio and Body Composition Analysis, fatness degree, in-body fat volume, in-body fat rate, BMI are the related indicators, which shows the connection with the fatness indicators. 4. In study on the correlation between Han-Yeol [寒熱] grade and Body Composition Analysis indicators, the result is that Han[寒] has no connection and that only Yeol[熱] grade has something to do with it, which means the higher heat symptom subjects have, the more basic metabolism volume and muscular build they have.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1998년도 춘계학술대회 논문집
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• pp.535-537
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• 1998

In this paper, we devised a pulse rate detection system to provide basic clinical index of cold-hot diagnosis of oriental medicine. The system consists of pulse signal detection, respiration signal detection, electrocardiograph detection, A/D conversion and computer system parts. We define a pulse rate by a pulse count to the respiration period inspiration pulse rate by a pulse count to the inspiration period, and expiration pulse rate by a pulse count to the expiration period. The clinical experiments for normal Person to evaluate the pulse rate detection system show the pulse/respiration ratio of 4.30${\pm}$1.03, the pulse/inspiration ratio of 1.60${\pm}$0.32, the pulse/expiration ratio of 2.37${\pm}$0.75.

• 대한한의진단학회지
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• 제12권1호
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• pp.103-130
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• 2008

Background: For standardizing of Han-Yeol [寒熱], which is a kind of diagnosis method in oriental medicine, it is necessary to investigate into relationship of symptoms and signs representative of Han-Yeol [寒熱] to the biofunctional medical signals; thermal temperature by Thermography, Pulse-Respiration Ratio and so on. By correlation analysis of these data items acquired from patients, it could be provides the fundamental data for standardizing of Han-Yeol [寒熱]. Objectives: We performed this study to check the characteristics of thermal temperature with Han-Yeol [寒熱] statue by pulse-respiration ratio. Methods: We selected nine regions around acupoints including Yin dang[印堂], Sugu[水溝, GV26], Ch'ondol[天突, CV22], Chonjung[CV17], Chung-wan[中脘, CV12], Chonchu[天樞 S25], No-gung[勞官, P8], and calculated based on the utility of R.O.I.(Region of Integer) by IR-2000 these points temperature from 68 subjects. In practicing pulse-respiration ratio over 4.0 means the statues of Yeol [熱], pulse-respiration ratio below 4.0 means the statues of Han [寒]. To optimum conditions thermal temperature, which are not effected by internal and external variables, we studied preceding research. The results shows that optimal time period is 20minutes after undressed and the optimal region is the region around acupoints including Sugu [水溝, GV26]. Based on a preceding research results, we analyzed these data by Paired T-test between GV26 Region and 8-Thermography Regions and two-way repeated ANOVA with thermography$({\Delta}T)$ and Han-Yeol [寒熱] statue by pulse-respiration ratio. Results: 1. In applying of two-way repeated ANOVA with thermography$({\Delta}T)$ and Han-Yeol [寒熱] statue by pulse-respiration ratio, Sugu [水溝穴, GV26] - [印堂穴, HN1], Sugu 水溝, GV26] - Chonjung[CV17], Sugu [水溝, GV26] -Chung-wan[中脘, CV12]had significant differences. 2. In applying of Paired T-test between Sugu [水溝穴, GV26] Region and 8- Thermography Regions, there were significant differences except of Sugu [水溝穴, GV26] -Chondolp[天突, CV22]. 3. In the difference of Sugu [水溝, GV26] -Chung-wan[中脘, CV12], thermal temperature increases a lot in the statues of Yeol [熱], thermal temperature decreases a lot in the statues of Han [寒]. Conclusions: On the analysis of Thermography, we obtain the measurement conditions were considered the individual variations. And it is different that the thermal temperature change on Thermography according to Han-Yeol [寒熱] statue by pulse-respiration ratio.

• 한국응급구조학회지
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• 제12권3호
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• pp.43-53
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• 2008

Purpose : This study was designed to examine physiological changes in the body of rescuers conduct CPR according to the 2005 new guideline from American Heart Association. The ratio of artificial respiration has changed from 15 : 2 into 30 : 2 in 2005. The researcher tried to know the correlation between the physiological changes and the accuracy of CPR. Method : The examinees of this study were 26 students (Dept. of Emergency Medical Service). After the training, participants conducted 10 minute CPR and soon after the CPR, their vital signs were checked, and lactic acid and concentration of ammonia were analysed from their blood samples. Questionnaires to ask their subjective fatigue level were filled out after blood samples and 10 minute - CPR was performed. Results : 1) After the CPR, concentrations of ammonia were $149.71{\mu}{\ell}/d{\ell}$ and $162.17{\mu}{\ell}/d{\ell}$ in 15 : 2 and 30 : 2, respectively. The number was higher in 30 : 2 but it wan not statistically meaningful (p = .493). Log value of lactic acid was a little higher in 30 : 2 with 42 log($mmol/{\ell}$) and 54 log($mmol/{\ell}$) in 15 : 2 and 30 : 2, respectively but it was not statistically meaningful (p = .113). 2) Blood pressure in 15 : 2 and 30 : 2 were 118.50 mmHg and 125.08 mmHg while pulse in two different cases were 96.14 and 97.25, showing no statistically significant differences (blood pressure : p = .155, pulse : p = .841). 3) Subjective fatigue was a bit high in 30 : 2 with 5.93 and 6.92 points in 15 : 2 and 30 : 2 respectively but it was not statistically meaningful (p = .142). 4) In the 10 minute CPR, respiration accuracy was 96.21% in 15 : 2 and 94.79% in 30 : 2. There was no statistical significances between the two(p = .225). In the meanwhile, chest compression accuracy was 92.57% in 15 : 2 and 91.83% in 30 : 2. From the beginning to the end of chest compression, there showed no difference(p = .425). the type of CPR did not influence upon the accuracy of chest compression(p = .756). Conclusion : In the CPR conducted by skilled rescuers for 10 minutes, there were no statistically meaningful differences between 15 : 2 and 30 : 2 in the concentration of fatigue element in a blood, subjective fatigue, vital signs and accuracy of CPR. Therefore, 30 : 2 CPR recommended by American Heart Association need to be recommended and performed in scene size up.

• 한국임상수의학회지
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• 제16권2호
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• pp.439-442
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• 1999

An animal health monitoring system in Gyeongnam area (near-Chinju) was studied to evaluate the environmental risk factors, physical examinations and 4 disease entities(abomasal displacement, traumatic reticulopericarditis ＆ -peritonitis, milk fever and lameness) in 40(34 in second year)dairy herds (total 1253 dairy cattle). In feeding environments, we examined housing system, forage percentage in ration, stall types, playground, cleanness of stall. In housing system, most of herds (60%) have tie-stall types and 36 herds are open-type housing. The forage ratio of ration was less than 50% in most of herds (67%). 39 herds had their own playgrounds and the frequency of playground cleanness was irregular, Physical examinations included the T(temperature), P (pulse), R (respiration), abnormalities of superficial lymph nodes, claw examination and total CBC with blood from tail veins. T, P, R are within normal limits (T : 38.1$\pm$0.6$^{\circ}C$, P : 84.6$\pm$12.9/min., R : 24.0$\pm$7.6/min. ,respectively), the swelling of lymph nodes were shown in 13 cattle and overall claw trimming was required in 3 herds. In blood examination, 23 cattle showed leuko-cytosis and 7 cattle showed low RBC and hemoglobin level, the other cattle were within normal limits (WBC : 8.90$\pm$2.06 10$^3$/ul., RBC : 6.36$\pm$1.02 10$^{6}$ ul, Hb : 9.83$\pm$ 1.20 g/dl PCV : 27.43$\pm$5.67 %, respectively). In 4 disease entities, we found some metallic foreign bodies in men of 13 cattle, which had predisposing factors of traumatic reticulopericarditis and reticuloperitonitis, 13 abomasal displacement, 51 milk fever and lameness in 39 cattle.

• 미생물학회지
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• 제41권1호
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• pp.67-73
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• 2005

본 연구에서는 남세균인 Synechocystis sp. PCC 6803 (Syn6803)을 대상으로 transposable element Tn5를 이용하여 획득된 1,200여 돌연변이주로부터 모균주에 비하여 PHB 축적량이 크게 증진된 균주를 선별하고, Tn5 삽입에 의해 결함을 나타낸 유전자를 확인함으로써 Syn6803에서의 PHB 생합성에 영향을 주는 세포내 생리학적 요인을 조사하고자 하였다. 모균주인 야생형 균주의 경우 질소원이 제한된 $BG11_0$ 배지에서의 PHB 생합성량이 건체량의 $4\%$ (w/w) 수준인데 반하여, $10-34\%$의 생합성량을 보이는 25개의 돌연변이 균주를 얻을 수 있었다. Inverse PCR을 이용하여, 선별된 돌연변이 균주내 돌연변이가 일어난 유전자를 조사한 결과, 아직까지 그 기능이 규명되지 않은 유전자가 대부분이었으나, NADH-ubiquinone oxidoreductase, O-succinylbenzoic-CoA ligase 또는 photosystem II PsbT protein과 같이 광합성과 호흡에 관여하는 유전자에 돌연변이가 일어난 4 균주와 histidine kinase가 결여된 1균주가 확인되었다. 이들 균주를 대상으로pulse-amplitude modulated fluorometer를 이용하여 세포내 $NAD(P)H/NAD(P)^+$비를 측정한 결과, 에너지 대사 흐름의 차단에 의해 세포내의 $NAD(P)HNAD(P)^+$비가 모균주에 비하여 현저하게 높은 것으로 나타났다. 이는 잉여의 전자로 포화된 세포, 즉 NAD(P)H에 의해 환원적 상태를 유지하고 있는 세포의 경우 PHB 축적 이 증진될 수 있음을 시사한다. 이러한 사실은 인위적으로 광합성과 호흡 관련 유전자가 제거되어 $NAD(P)H/NAD(P)^+$비가 높아진 것으로 알려진 다수의 Syn6803 돌연변이 균주들을 대상으로 PHB 생합성량을 조사한 결과로부터 재확인되었다.