• 제목/요약/키워드: Pseudopodia

검색결과 14건 처리시간 0.017초

이질(痢疾)아메바(Entamoeba histolytica)의 면역혈청(免疫血淸) 내(內)에서의 미세구조(微細構造) 변화(變化) (Ultrastructural Changes of Entamoeba histolytica in the Immune Serum)

  • 등영건;정평림;장재경;소진탁
    • Applied Microscopy
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    • 제7권1호
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    • pp.1-12
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    • 1977
  • Ultrastructural changes of Entamoeba histolytica, a concomitant strain YS-9, which was treated in the immune serum was examined. The amoebae in the serum became immobilized state from about 30 minutes of the treatment and recovered at about 60-90 minutes. In the cells of control group, helix structures were scattered throughout the cytoplasm. The particles comprising the helix structure averaged 20 nm in diameter. At the beginning stage of the immobilization, helical aggregates(chromatoid body) which associated with vacuoles appeared abundantly in the cytoplasm, but gradually tended to aggregate along peripheral region of the cell, specially in intactly immobilized state. Each parallel array of aggregates measured about 45 nm in width. When the cells became remobilize, pseudopodia appeared again, but helical aggregates disappeared and numerous helix structures were observed in the cell periphery. Distribution of glycogen particles showed no change, and acid phosphatase activities were seen in both the immobilized and the control group. The reaction was markedly noticed in the vacuoles.

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Synchrotron-based Transmission X-ray Microscopy (TXM) Observations of Fully Hydrated Blood Platelets and Their Activation Process

  • Yang, Nuri;Nho, Hyun Woo;Kalegowda, Yogesh;Kim, Jin Bae;Song, Jaewoo;Shin, Hyun-Joon;Yoon, Tae Hyun
    • Bulletin of the Korean Chemical Society
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    • 제35권9호
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    • pp.2625-2629
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    • 2014
  • Platelets are anuclear discoid-shaped blood cells with key roles in human body. To understand the mechanisms of their activation process, it is required to have analytical imaging techniques capable of acquiring platelet images under fully hydrated conditions. Herein, for the first time, we demonstrate the capability of synchrotron-based transmission X-ray microscopy (TXM) to study platelets (resting and ADP activated) under hydrated and air-dried conditions. To confirm the biological imaging capability of TXM, fixed platelets were imaged and compared with whole mount electron microscopy (EM) images. TXM provided morphological information with sufficient spatial resolution with simple and quick sample preparation procedure. We also observed temporal changes during the platelet activation, which initially had a discoid shape (0 s), formed pseudopodia (30 s) and generated a network of fibrin (5 min). Our results clearly demonstrate the potential of TXM technique to study fully hydrated biological samples under in situ conditions.

경골에 발생한 거대 골 섬 - 증례 보고 - (Giant Bone Island of the Tibia - A Case Report -)

  • 박호성;장규윤;이상용;김정렬
    • 대한골관절종양학회지
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    • 제10권1호
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    • pp.56-60
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    • 2004
  • 골 섬 (bone island)은 해면골 내에 성숙된 치밀골 병소가 존재하는 것이다. 골 섬은 특징적인 임상적 및 방사선학적 소견으로 진단될 수 있다. 병변은 무증상인 경우가 많으며 골반, 대퇴골, 그리고 다른 장골에서 호발한다. 방사선 검사상 병변은 해면골 내에 난원형, 원형, 또는 길게 늘어진 모양의 균질하고 진한 경화성 병변으로 관찰되며 "가시 방사" 또는 "위족"으로 알려진 방사상 뼈 줄무늬가 특징적인 소견이다. 대부분의 골 섬은 직경이 0.1 cm 부터 2.0 cm 로 크기가 작다. 직경이 2 cm 이상으로 정의된 거대 골 섬은 보고가 극히 드물다. 저자들은 31세 남자에서 발생한 10${\times}$1.7${\times}$1 cm 크기의 거대 골 섬 1예를 보고하고자 한다.

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Effects of Tiam 1 on Invasive Capacity of Gastric Cancer Cells in vitro and Underlying Mechanisms

  • Zhu, Jin-Ming;Yu, Pei-Wu
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권1호
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    • pp.201-208
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    • 2013
  • Objective: To investigate changes in the invasive capacity of gastric cancer cells in vitro after expression inhibition of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) and underlying mechanisms. Methods: Using adhesion selection, two subpopulations with high ($M_H$) or low ($M_L$) invasive capacity were separated from the human gastric cancer cell line MKN-45 ($M_0$). Tiam 1 antisense oligodeoxynucleotide (ASODN) was transfected into $M_H$ cells with liposomes, and expression of Tiam 1 mRNA and protein was determined by RT-PCR and quantitative cellular-ELISA. Changes in the cytoskeleton, invasive capacity in vitro and expression of ras-related $C_3$ botulinum toxin substrate 1 (Rac 1), integrin ${\beta}1$ and matrix metalloproteinase 2 (MMP 2) between Tiam 1 ASODN transfected $M_H$ cells and non-transfected cells were observed by HE staining, cytoskeletal protein staining, scanning electron microscopy, Boyden chamber tests and cyto-immunohistochemistry. Results: A positive correlation existed between the expression level of Tiam l mRNA or protein and the invasion capacity of gastric cancer cells. After ASODN treatment ($0.43{\mu}M$ for 48 h), Tiam 1 mRNA transcription and protein expression in $M_H$ cells were decreased by 80% and 24% respectively (P < 0.05), compared with untreated controls, while invasive capacity in vitro was suppressed by 60% (P < 0.05). Morphologic and ultrastructural observation also showed that ASODN-treated $M_H$ cells exhibited smooth surfaces with obviously reduced filopodia and microspikes, which resembled $M_0$ and $M_L$ cells. Additionally, cytoskeletal distribution dramatically altered from disorder to regularity with reduced long filament-like structure, projections, pseudopodia on cell surface, and with decreased acitn-bodies in cytoplasm. After Tiam 1 ASODN treatment, the expression of Rac 1 and Integrin ${\beta}1$ in $M_H$ cells was not affected (P > 0.05), but that of MMP 2 in $M_H$ cells was significantly inhibited compared with untreated cells (P < 0.05). Conclusion: Over-expression of Tiam-1 contributes to the invasive phenotype of gastric cancer cells. Inhibition of Tiam 1 expression could impair the invasive capacity of gastric cancer cells through modulating reconstruction of the cytoskeleton and regulating expression of MMP 2.