• 제목/요약/키워드: Pseudomonas sp. RP-222

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Pseudomonas sp. RP-222와 변이주 MR-3966 의 생육 및 Protease 생산에 Cysteine이 미치는 저해효과 (Inhibitory Effects of Cysteine on Growth and Protease Production of Preudomonas sp. RP-222 and its Mutant MR-3966)

  • 이광수;강신권;손봉수;노종수;김경숙;전성식;성낙계
    • 한국미생물·생명공학회지
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    • 제22권6호
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    • pp.621-626
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    • 1994
  • Cysteine showed strong inhibitory effect on growth and protease production of Pseudo- monas sp. RP-222 and its mutant, MR-3966. Mid- to late-log phase cells were most sensitive to the presence of 10 mM cysteine. The inhibition caused by cysteine was almost completely overcome by addition of isoleucine, leucine and valine mixture to the medium, and inclusion of iso#leucine alone could greatly reduce the inhibitory effects of cysteine. Homocysteine and #cysteine, sulfur compounds having similar structure as cysteine, inhibited to varying degrees the growth of both strains. Cysteine and homocysteine were strong inhibitors of threonine deaminase but not transa#- minase B. These results suggest a relationship between the growth-inhibitory effects of cysteine and other sulfur compounds and the inhibition of isoleucine synthesis at the level of threonine deaminase.

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저온.알칼리성 Protease를 생산하는 Pseudomonas sp. RP-222의 분리 및 조효소의 특성 (Isolation of Alkalopsychrotrophic Protease-Producing Pseudomonas sp. RP-222 and Properties of Its Crude Enzyme)

  • 노종수;정영철;성낙계;박석규
    • 한국미생물·생명공학회지
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    • 제19권4호
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    • pp.383-389
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    • 1991
  • 저온에서 높은 활성을 나타내는 알칼리성 protease를 생산하기 위하여 여러가지 시료로부터 집적배양에 의해 저온성 세균을 분리하였다. 분리된 세균은 저온.알칼리성 Pseudomonas sp.인 것으로 판명되었으며, 효소생산을 위한 균생육의 최적 pH는 10.0, 온도 $20^{\circ}C$에서 4일간 배양하였을 때였다. 이 효소 활성의 최적 pH 및 온도는 각각 pH 10.5 및 $40^{\circ}C$였으며, pH 및 열안정성은 각각 pH 7.0~13.0, 온도 $50^{\circ}C$이하의 범위에서 비교적 안정하였다.

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