• 제목/요약/키워드: Protogynous

검색결과 11건 처리시간 0.03초

Early Gonadal Differentiation of the Protogynous Red Spotted Grouper, Epinephelus akaara

  • Kim, Hyun Kyu;Kim, Jung-Hyun;Kim, Woo Sik;Baek, Hea Ja;Kwon, Joon Yeong
    • 한국발생생물학회지:발생과생식
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    • 제19권4호
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    • pp.209-215
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    • 2015
  • Red spotted grouper, Epinephelus akaara is a popular aquaculture species in many Asian countries. This species is a protogynous hermaphrodite that first differentiates into female and changes to male later. Due to this reproductive characteristic, stable supply of male and female gametes is a key to the success of seed production in this species. Thus, understanding early gonadal differentiation is required to develop effective sex control techniques. Red spotted grouper were reared in indoor tanks and sampled every 5 days from 40 days post-hatch (DPH) to 130 DPH. Changes of gonadal tissues were examined and analyzed by means of histology. A pair of gonadal primordium has already existed underneath the kidney in the posterior part of the body cavity at 38 DPH when this study began. Gonadal primordia of 38, 40 DPH consisted of germ cells surrounded by a few somatic cells. The blood vessel was observed in the gonadal primordium at 45 DPH. The number of somatic cells and size of gonadal primordium increased age-dependently up to 60 DPH. Formation of ovarian cavity was obvious by two protuberant aggregations of somatic cells at 65 DPH. Completed ovarian cavity and oogonia were first observed in the gonad of one fish sample at 105 DPH. Based on these histological observations, it can be suggested that induction of primary male differentiation could be more successfully applied at around 60 DPH in this species.

제주 연안에 서식하는 놀래기류의 성 특성 (Sex Characterization of Wrasses Inhabiting in the Coastal Waters of Jeju, Korea)

  • 이치훈;김윤석;이영돈
    • 한국발생생물학회지:발생과생식
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    • 제10권2호
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    • pp.79-84
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    • 2006
  • 제주 연안에 서식하는 용치놀래기, 어렝놀래기, 황놀래기, 놀래기를 대상으로 체장에 따른 성 분포와 생식소의 성 특성을 조직학적으로 탐색하여 성 전환 양상을 조사 비교하였다. 용치놀래기, 어렝놀래가, 황놀래기, 놀래기는 자성선숙형 자웅동체어로 조직학적 관찰 결과 정소조직(정자형성부위)이 퇴화기 난소의 전역에 출현하는 혼재형 2에 속하는 어종이었다. 용치놀래기, 황놀래기 그리고 놀래기는 1차 수컷과 2차 수컷이 존재하는 복웅성 어류이고, 조사된 어렝놀래기는 암컷이 수컷으로 성 전환된 2차 수컷만이 출현하는 단웅성 어류로 사료된다.

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Gene Expression of Aromatases, Steroid Receptor, GnRH and GTHs in the Brain during the Formation of Ovarian Cavity in Red Spotted Grouper, Epinephelus akaara

  • Kim, Hyun Kyu;Kim, Jung-Hyun;Baek, Hea Ja;Kwon, Joon Yeong
    • 한국발생생물학회지:발생과생식
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    • 제20권4호
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    • pp.367-377
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    • 2016
  • Red spotted grouper, Epinephelus akaara, is a popular aquaculture species and a protogynous hermaphrodite. Induction of artificial sex change at the time of primary sex differentiation is of interest but has not been successful due to the lack of necessary basic information. To find out the potential neuroendocrine influence on the primary sex differentiation, the expression of key genes in the brain was investigated during the formation of ovarian cavity. Expression of cyp19a1b, esr1, gnrhr1, fsh, lh and cga in the brain was positively associated with the formation of ovarian cavity, showing gradual increase as the formation proceeds. However, the expression of gnrh1 was suppressed during the early part of the ovarian cavity formation, signifying potential hypothalamic influence on the primary sex differentiation in this species.

Molecular Cloning and Expression of DMRT Gene in Protogynous Wrasse, Halichoeres tenuispinis

  • Jeong, Hyung-Bok;Park, Ji-Gweon;Park, Jin-Young;Jin, Young-Jun;Yang, Myung-Cheon;Hyun, Kyung-Man;Kim, Gi-Ok;Kim, Se-Jae
    • 한국발생생물학회:학술대회논문집
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    • 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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    • pp.64-64
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    • 2003
  • The sex differentiation of fishes occurs under the control of genetic and various environmental factors. DM-domain containing genes are novel zinc finger transcription factors and play key roles in sex determination. In order to isolate the wrasse DMRT (wDMRT) cDNA from the protogynous wrasse (Halichoeres tenuispinnis), the wrasse testis cDNA library was screened using the $^{32}$ P-labeled PCR products, which were amplified with the degenerate primers from conserved DM-domain regions of several DMRT genes. Among a few positives obtained through screening, the full length wDMRT cDNA of 2.9kb size encoding a predicted 300 amino acid residues was isolated. The sequence analysis exhibited 60%, 43% sequence identity with rainbow trout and tilapia DMRT1, respectively. RT-PCR assay showed that wDMRT was expressed specifically in male testis. Also, wDMRT gene was strongly expressed in May during reproductive season, when the reproductivity of wrasse is most active. This results suggested that wDMRT gene function in testis differentiation The conserved DM-domain regions were amplified using PCR from DMRT genes of several species among Labridae, and their sequences were determined. The sequence of DM-domain region of Halichoeres. tenuispinis was identical to those of Pseudolabrus japonicus, Pteragogus flagellifera, and showed 94% identity with that of Halichoeres poecioptrerus.

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Micronesia, Chuuk Lagoon에 서식하는 파랑돔(Pomacentrus coelestis)의 생식년주기 (Annual Reproductive Cycle of the Neon Damselfish Pomacentrus coelestis, in Chuuk Lagoon, Micronesia)

  • 김한준;한정훈;허성표;이균우;최영웅
    • 한국수산과학회지
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    • 제54권2호
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    • pp.180-187
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    • 2021
  • This study was conducted to investigate the reproductive characteristics of the Neon damselfish Pomacentrus coelestis in Chuuk lagoon (7°27' N, 151°53' E), Micronesia, Equatorial Pacific Ocean. The specimens used in this study were collected from August 2009 to September 2010 from around the coral reef area at a depth of 3-10 m in the northwest coast of Weno Island. Overall, 228 individuals were collected over 14 months from this area, of which 115 were females and 108 were males. Among 108 males, 30 were found to be hermaphrodites and the sex of 5 individuals could not be determined. The overall sex ratio was approximately 1.06:1 (female:male), and the ratio of sexually active individuals was 0.79:1. The gonadosomatic index (GSI) fluctuated and showed the highest value of 8.43±1.21 in December 2009 and the lowest value of 3.36±0.71 in September 2009 in sexually active females. Males showed the highest GSI value of 2.64±0.93 in June 2010 and the lowest value of 0.35±0.06 in July 2010. Based on the fluctuation of GSI, the trends in the appearance of monthly sexually active individuals, and biopsy of the gonads, P. coelestis was found to be a hermaphrodite species of protogynous type and is considered to be reproductively active throughout the year.

단풍나무의 성 형태, 결실특성 및 종자활력 (Sex Morph, Fruiting Habit, and Seed Viability of Acer palmatum)

  • 김갑태;김회진
    • 한국산림과학회지
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    • 제101권1호
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    • pp.91-95
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    • 2012
  • 단풍나무의 이형자웅이숙, 성형태의 다양성 및 결실특성을 파악하고자, 원주지역에 생육 중인 단풍나무(Acer palmatum) 63주를 대상으로 화기구조, 수분시스템, 결실특성 시과활력을 2010년 4월부터 2011년 10월까지 조사하였다. 단풍나무는 주로 꿀벌과 꽃등에 등의 화분매개충에 의해 수분되나, 소수(3.57%)는 바람에 의해 수분되었다고 추정된다. 조사대상목 중에서 2010년 24개체(42.9%), 2011년 20개체(31.7%)는 웅예선숙개체(Protandrous, PA)였으며, 2010년 32개체(50.8%), 2011년 30개체(47.6%)는 자예선숙개체(Protogynous, PG)로 단풍나무는 이형자웅이숙(異型雌雄異熟: heterodichogamy)하는 것이 밝혀졌다. 5개체(7.9%)에서 성전환-4개체는 PA에서 PG로, 1개체는 PG에서 PA로-이 확인되었다. 건전종자와 부후종자의 비율에서는 성 형태 간의 통계적 유의성이 인정되었다. 건전종자의 비율은 PA에서 2010년 25.8/50, 2011년 32.0/50로 상대적으로 높게 나타났고, PG에서는 2010년 15.2/50, 2011년 20.2/50로 상대적으로 낮게 나타났다. 부후종자의 비율은 PA에서 2010년 20.0/50, 2011년 15.6/50로 상대적으로 낮게 나타났고, PG에서는 2010년 32.8/50, 2011년 28.4/50로 상대적으로 높게 나타났다. 성형태간의 부후종자율의 차이는 단풍나무 종실가해 해충들의 활동시기와 관련이 있을 것이라 사료된다.

뜰단풍의 성 형태, 결실특성 및 종자활력 (Sex Morph, Fruiting Characteristics, and Seed Viability of Acer palmatum var. matsumurae (Koidz.) Makino)

  • 김갑태
    • 한국산림과학회지
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    • 제100권2호
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    • pp.131-135
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    • 2011
  • 단풍나무속에 흔하다고 알려진 이형자웅이숙현상을 파악해 보고자, 원주지역에 생육 중인 뜰단풍(Acer palmatum var. matsumurae) 101주를 대상으로 성 형태, 결실특성 및 종자활력에 대하여, 2010년 4월 초순부터 10월 하순까지 조사하였다. 101주의 뜰단풍 중에서 74개체(73.3%)에서는 수꽃이 먼저 개화하고, 암술은 흔적기관인 웅예선숙(雄蕊先熟)개체 (Protandrous, PA)였으며, 27개체(26.7%)는 암꽃이 먼저 개화하고, 수술이 소실되는 웅예선숙(雄蕊先熟) 개체(Protgynous, PG)로 뜰단풍은 이형자웅이숙(異型雌雄異熟: heterodichogamy)하는 것이 밝혀졌다. 성형태에 따른 화서당 꽃 수와 종자셑 수는 통계적 유의차가 인정되었으며, 웅예선숙 개체가 자예선숙 개체보다 많았다. 건전종자와 부후종자의 비율에서는 성 형태 간의 통계적 유의성이 인정되었다. 건전종자의 비율이 웅예선숙 개체에서 46.4%였으며, 자예선숙 개체에서는 65.6%로 나타났다. 부후종자의 비율은 웅예선숙 개체에서는 49.6%였으며, 자예선숙 개체에서는 23.6%로 나타났다. 성형태간의 부후종자율의 차이는 암꽃의 개화시기와 종실가해 해충들의 활동시기와 관련이 있을 것이라 사료된다.