• Title/Summary/Keyword: Protein quality control

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THE UTILIZATION OF FULL FAT SOYBEAN FOR EGG PRODUCTION AND EGG QUALITY IN THE LAYING HENS

  • Han, I.K.;Choi, Y.J.;Chu, K.S.;Park, H.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.1 no.3
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    • pp.173-178
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    • 1988
  • To study the effects of utilizing extruded full fat soybean (FFS) in layer diets, 300 White Leghorn pullets were put in trial divided into 5 treatments; control (commercial soybean meal as protein source), FFS 50% (replacing 50% of soybean meal with FFS), FFS 100% (replacing all amount of soybean meal with FFS), soybean oil and tallow added treatment. Each treatment consisted of 5 replicates of 12 birds. Egg production and feed efficiency from hens fed control diet were significantly lower than from hens fed the other diets. Significant differences in egg production and feed efficiency were found between the control and the other treatments. Cholesterol concentration of serum and egg yolk were not affected by any dietary treatment. Feeding extruded full fat soybean did not cause pancreatic hypertrophy nor change in mortality. There was an indication that linoleic acid (C18:2), linolenic acid (C18:3) and iodine contents increased in the thigh and egg yolk lipid of the groups fed FFS or soybean soybean can serve as effective protein source for layer diets if economically justified.

Dynamic changes and characterization of the protein and carbohydrate fractions of native grass grown in Inner Mongolia during ensiling and the aerobic stage

  • Du, Zhumei;Risu, Na;Gentu, Ge;Jia, Yushan;Cai, Yimin
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.4
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    • pp.556-567
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    • 2020
  • Objective: To improve the utility of native grass resources as feed in China, we investigated the dynamics of protein and carbohydrate fractions among Inner Mongolian native grasses, during ensiling and the aerobic stage, using the Cornell Net Carbohydrate and Protein System. Methods: Silages were prepared without or with lactic acid bacteria (LAB) inoculant. We analyzed the protein and carbohydrate fractions and fermentation quality of silages at 0, 5, 15, 20, 30, and 60 d of ensiling, and the stability at 0.5, 2, 5, and 10 d during the aerobic stage. Results: Inner Mongolian native grass contained 10.8% crude protein (CP) and 3.6% water-soluble carbohydrates (WSC) on a dry matter basis. During ensiling, pH and CP and WSC content decreased (p<0.05), whereas lactic acid and ammonia nitrogen (N) content increased (p<0.05). Non-protein N (PA) content increased significantly, whereas rapidly degraded true protein (PB1), intermediately degraded true protein (PB2), total carbohydrate (CHO), sugars (CA), starch (CB1), and degradable cell wall carbohydrate (CB2) content decreased during ensiling (p<0.05). At 30 d of ensiling, control and LAB-treated silages were well preserved and had lower pH (<4.2) and ammonia-N content (<0.4 g/kg of fresh matter [FM]) and higher lactic acid content (>1.0% of FM). During the aerobic stage, CP, extract ether, WSC, lactic acid, acetic acid, PB1, PB2, true protein degraded slowly (PB3), CHO, CA, CB1, and CB2 content decreased significantly in all silages, whereas pH, ammonia-N, PA, and bound true protein (PC) content increased significantly. Conclusion: Control and LAB-treated silages produced similar results in terms of fermentation quality, aerobic stability, and protein and carbohydrate fractions. Inner Mongolian native grass produced good silage, nutrients were preserved during ensiling and protein and carbohydrate losses largely occurred during the aerobic stage.

Biosynthesis of Organic Germanium Using Cordyceps militaris (동충하초를 이용한 유기게르마늄의 생산)

  • Kim, Seung;Kim, Jae-Sung;Sapkota, Kumar;Choi, Bong-Suk;Park, Se-Eun;Park, Yeal;Chun, Hong-Sung;Yoo, Jin-Cheol;Choi, Han-Seok;Kim, Myung-Kon;Kim, Sung-Jun
    • The Korean Journal of Mycology
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    • v.34 no.2
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    • pp.84-87
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    • 2006
  • In the present study, an attempt has been made to produce a high quality medicinal mushroom Cordycops militaris supplemented with organic Ge. Cordycops militaris was cultivated in SDAY liquid medium containing yeast extract 10 g, peptone 10 g, glucose 40 g per liter and chrysalis media supplemented with inorganic Ge at 100, 500, 1000, and 5000 ppm concentrations. The greatest organic Ge production of 442.4 ppm/g and 284 ppm/g were observed in SDAY liquid and Chrysalis media cultures supplemented with 100 ppm inorganic Ge respectively. Similarly, 4,509.7 ppm/g and 1,058 ppm/g of organic Ge were obtained from liquid and chrysalis media cultures supplemented with 5,000 ppm and 1,000 ppm inorganic Ge, respectively. In addition, higher concentration of organic Ge was obtained in mycelia than fruiting bodies. These results indicate that the concentration of organic Ge increase with decreasing inorganic Ge concentration in the medium. This is the first report on production of high valuable Cordyceps militaris contained with organic Ge.

Effects of Wheat Fiber on the Quality of Meat Batter (Wheat Fiber 첨가가 Meat Batter의 품질에 미치는 영향)

  • Choi, Yun-Sang;Lee, Mi-Ai;Jeong, Jong-Youn;Choi, Ji-Hun;Han, Doo-Jeong;Kim, Hack-Youn;Lee, Eui-Soo;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.27 no.1
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    • pp.22-28
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    • 2007
  • The purpose of this study was to investigate the effect of wheat fiber as a replacement for isolated soy protein (ISP) or concentrated soy protein (CSP) on the quality characteristics of meat batter. Meat batter formulations were prepared with 2% levels of ISP, CSP, or wheat fiber. The pH values of each formulation were not significantly different (p>0.05). The L-values of meat batter containing wheat fiber, ISP, or CSP were higher than the control, however the a- and b-values were lower than the control (p<0.05). Relative to the control meat batter, the water holding capacity, viscosity, hardness, gumminess, and chewiness of meat batter containing ISP, CSP, or wheat fiber was significantly higher than the control (p<0.05). All supplemented meat batters had significantly lower cooking loss and emulsification stability than the control (p<0.05), with no significant difference among the supplemented meat batters. The results of this study show that the use of wheat fiber as an ISP or CSP replacer does not affect the overall quality of meat batter.

Effects of Resistance Training on Serum Inflammatory Markers and CatSper 1-4 Protein Expression in Testis of OLETF Rats (저항성 운동이 OLETF 쥐의 혈청 염증반응지표 및 고환조직의 CatSper 1-4 단백질 발현에 미치는 영향)

  • Lee, Min-Ki;Park, Se-Hwan;Yoon, Jin-Hwan
    • Exercise Science
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    • v.26 no.3
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    • pp.204-211
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    • 2017
  • PURPOSE: This study was to investigate the effects of resistance exercise on serum inflammatory markers and CatSper 1-4 expression in testis of OLETF rats. METHODS: Male OLETF rats were divided into two groups; control group (n=12), resistance exercise group (n=12). The exercise group performed a total of 8 weeks of moderate intensity resistance exercise on a 1.35 m vertical ladder with weights secured to their tails. RESULTS: The results of this study was following; The exercise group showed a significant decreased in the inflammatory ($IL-1{\beta}$, IL-6, $TNF-{\alpha}$) levels as compared to the control group. But CRP was no significant difference between control and exercise groups. Also, CatSper 1 and 2 were significantly increased in the exercise group compared to the control group, whereas CatSper 3, 4 were no significant difference between both groups. CONCLUSIONS: This study suggests that resistance exercise training can contribute to reduce pro-inflammatory responses in whole body and it affects male reproductive function by improve sperm quality and CatSper protein expression.

Evaluation of Nutritional Quality of Packaged MealsProduced by Packaged Meal Manufactures in Seoul and Kyungki-do (시판 도시락의 영양평가 및 품질관리 방안에 관한 연구)

  • 계승희
    • Journal of Nutrition and Health
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    • v.22 no.3
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    • pp.149-158
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    • 1989
  • To investigate the nutritional quality of packaged meals produced by packaged meal manufactures, a nutritional evaluation was conduced. Samples examined in this study were 95 Products which composed of 86 Doraks, 7 Kimplabs and 2 Yubuchopabs Produced in packaged meal manufactures in Seoul City and Kungki-do province The distribution of food groups showed that amounts of animal foods were abound, but amounts of green vegetables, limited in Dosirak comparing to one meal RDA levels. Amounts of meats, fishes and shells are increased more than amounts of egged and legumina with increase of price of Dosirak. The total kinds of food and the amounts of certain food in Kimpab and Yubuchopab were lower than Dosirak. The energy and protein contents of Dosirak were found to be higher than one meal RDA levels, with exception of protein content in Dosirak of the price 1000 won. The contents of energy and protein in Kimpab and Yubuchopab are less than one meal RDA levels. Calcium and iron contents in Dosirak, and calcium content in Yubuchopab were present in excess compared to the one meal RDA. However, In Kimpab, calcium and iron contents were lower than the one meal RDA. On the whole, vitamin contents of Dosirak were sufficient whereas vitamin contents of Kimpab and Yubuchopab were lower than the one meal RDA. Number of side dishes used in Dosirak were 6-23 items depending on prices. Guidelines were suggested for effective nutritional quality control of packaged meals.

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Study on the rumen fermentation, growth performance and carcass characteristics according to the supplementation of lupin flake in Hanwoo steers

  • Kyung-Hwan, Um;Byung-Ki, Park
    • Journal of Animal Science and Technology
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    • v.64 no.6
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    • pp.1077-1091
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    • 2022
  • This study was conducted to determine the rumen fermentation dynamics of lupin flakes and elucidate the effects of lupin flake supplementation on the growth performance, blood metabolites, and carcass characteristics of Hanwoo steers. In vitro and in situ trials of lupin grains and lupin flakes were conducted using three Hanwoo cows with rumen fistulas. The feeding trial included 40 early-fattening Hanwoo steers randomly divided into four groups: control, T1, T2, and T3. Their formula feed contained 0%, 3%, 6%, and 9% lupin flakes, respectively. In vitro rumen pH and ammonia concentrations were lower in the lupin flake group than in the lupin grain group after 6 and 24 h of incubation, respectively (p < 0.05). Concentrations of propionate, butyrate, and total volatile fatty acids were higher in the lupin flake group than in the lupin grain group after 12 h of incubation (p < 0.05), as was the crude protein disappearance rate at 9 and 12 h of rumen fermentation (p < 0.05). Supplementation with lupin flakes did not affect the average daily gain. Compared to that in the control group, dry matter intake was lower in the lupin flake-supplemented groups (p < 0.05); the feed conversion ratio was lower in T2 and T3 (p < 0.05); and plasma total protein concentration in 29-month-old steers was lower in T1 and T3 (p < 0.05). Plasma triglyceride concentration was lower in the lupin flake-supplemented groups than in the control group (p < 0.05). The incidence rate of yield grade A was higher in T1 and T2 than in the control group; the incidence rate of meat quality 1+ grade or higher was highest in T2. The carcass auction price was higher in T2 than in the other groups. Overall, compared to whole lupin grains, lupin flakes seem to more substantially affect rumen ammonia concentrations and crude protein disappearance rate. Additionally, we suggest that supplementation with 6% lupin flake formula feed exerts positive effects on the feed conversion ratio, yield grade, and quality grade of Hanwoo steers.

Effects of Supplementing Organic Citrus in Making Rice Straw Silage (볏짚사일리지 제조를 위한 유기감귤 첨가효과)

  • Ahn, Jong-Ho
    • Korean Journal of Organic Agriculture
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    • v.15 no.3
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    • pp.309-318
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    • 2007
  • The effects of supplementation of organic citrus on the quality of rice straw silage was investigated in this study. The aim of the study was to improve the quality of rice straw silage using agricultural by-products and therefore to secure the good quality forage sources. Firstly, looking at chemical composition of citrus added rice straw silage, crude protein content appeared improved with the addition of citrus to rice straw silage compared to control while NDF and ADF contents decreased. pH of the silages were different between control(4.39) and treatments$(3.47\sim3.53)$. Lactic acid content was higher in citrus added silage(4.87mM) than in control (3.65mM). External quality of the silages was also better in citrus added silage when citrus added at the equal ratio with rice straw(1 : 1). This trend was the same as with pH and lactic acid content. Propionate was significantly higher in citrus addd silage but butyrate was significantly lower. DM and NDF disappearance rates in the rumen were highest when citrus added at the equal ratio with rice straw(1:1). In conclusion, supplementation of citrus to rice straw silage with equal ratio contributed the best to the improvement of the quality of silage and it may have been due mainly to abundant content of non-structural carbohydrate in citrus.

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Roles of Heat Shock Protein gp96 in the ER Quality Control: Redundant or Unique Function?

  • Yang, Yi;Li, Zihai
    • Molecules and Cells
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    • v.20 no.2
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    • pp.173-182
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    • 2005
  • Heat shock protein gp96 is an endoplasmic reticulum chaperone, belonging to the HSP90 family. The function of gp96 as a molecular chaperone was discovered more than 10 years ago, but its importance has been overshadowed by the brilliance of its role in immune responses. It is now clear that gp96 is instrumental in the initiation of both the innate and adaptive immunity. Recently, the roles of gp96 in protein homeostasis, as well as in cell differentiation and development, are beginning to draw more attention due to rapid development in the structural study of HSP90 and some surprising new discoveries from genetic studies of gp96. In this review, we focus on the aspect of gp96 as an ER molecular chaperone in protein maturation, peptide binding and the regulation of its activity.

EFFECTS OF CIMATEROL (CL 263,780) ON GROWTH PERFORMANCE AND CARCASS QUALITY OF BROILERS FED ON DIFFERENT LEVELS OF DIETARY PROTEIN AND ENERGY

  • Kim, Y.Y.;Han, I.K.;Ha, J.K.;Choi, Y.J.;Lee, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.4 no.4
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    • pp.383-393
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    • 1991
  • The present study was carried out to investigate the effect of cimaterol on growth performance, carcass quality and cellular functional activity of broilers as affected by the various protein and energy levels. In starter period (0-21 days) all chicks were fed the basal diet which contained approximately 23 % crude protein and 3200 kcal of metabolizable energy per kg of diet. The cimaterol was added during 22-49 days and during the period of 8th week the cimaterol was withdrawn. In finisher period (22-49 days), a $2{\times}2{\times}3$ factorial arrangement consisting of 2 levels of cimaterol (0 mg/kg, 0.25 mg/kg), 2 levels of protein (19%, 17%) and 3 levels of energy (3200, 2900, 2600 kcal/kg) was used. In the finisher period, the body weight gain and feed efficiency was improved by the supplementation of cimaterol. The high protein and high energy level with supplementation of cimaterol had showed the highest body weight gain and feed efficiency, without significant difference. The administration of cimaterol had no effects on percentage of abdominal fat content, giblet and neck. Eventhough the difference was not significant (p>0.05), carcass yield was improved slightly by the administration of cimaterol. The effect of cimaterol on carcass composition was clearly demonstrated that protein content of broilers was not increased (p>0.05) but fat content decreased significantly (p<0.05). The ultilization of nutrients in experimental diets was not significantly affected by feeding cimaterol compared to control group. The results of in vitro studies with liver and adipose tissue showed that cimaterol increased the lipolytic activities at 19% protein level whereas at 17% protein level this effect was variable. Lipogenic activities in liver and adipose tissue were not affected with the administration of cimaterol but the activities increased as energy decreased, particularly in liver tissue. In cell studies with acinar culture of liver tissues, cimaterol had no effect on protein synthetic activity but the parameter was increased at higher level of dietary protein and energy. Protein secretion in liver was increased by the supplementation of cimaterol. In addition, at high protein level the protein secretion was increased and has shown the highest values at medium energy level.