• Mycobiology
• /
• 제40권4호
• /
• pp.236-243
• /
• 2012

Pleurotus nebrodensis is an edible and commercially available mushroom in Korea. This study was conducted in order to evaluate the anticancer and immunopotentiating activities of crude polysaccharides, extracted in methanol, neutral saline, and hot water (hereafter referred to as Fr. MeOH, Fr. NaCl, and Fr. HW, respectively) from the fruiting bodies of P. nebrodensis. ${\beta}$-Glucan and protein contents in Fr. MeOH, Fr. NaCl, and Fr. HW extracts of P. nebrodensis ranged from 23.79~36.63 g/100 g and 4.45~6.12 g/100 g, respectively. Crude polysaccharides were not cytotoxic against sarcoma 180, HT-29, NIH3T3, and RAW 264.7 cell lines at a range of $10{\sim}2,000{\mu}g/mL$. Intraperitoneal injection with crude polysaccharides resulted in a life prolongation effect of 11.76~27.06% in mice previously inoculated with sarcoma 180. Treatment with Fr. NaCl resulted in an increase in the numbers of spleen cells by 1.49 fold at the concentration of $50{\mu}g/mL$, compared with control. Fr. HW improved the immuno-potentiating activity of B lymphocytes through an increase in alkaline phosphatase activity by 1.65 fold, compared with control at $200{\mu}g/mL$. Maximum production of nitric oxide ($14.3{\mu}M$) was recorded in the Fr. NaCl fraction at $200{\mu}g/mL$. Production of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), and interleukin-6 (IL-6) was significantly higher, compared to control, and IL-6 production was highest, in contrast to TNF-${\alpha}$, IL-$1{\beta}$, and positive control, concanavalin at the tested concentration of the various fractions. Results of the current study suggest that polysaccharides extracted from P. nebrodensis have a strong anticancer effect and may be useful as an ingredient of biopharmaceutical products for treatment of cancer.

• 동의생리병리학회지
• /
• 제23권3호
• /
• pp.715-722
• /
• 2009

The aim of this study was to investigate the safety of short-term use of Korean herbal medicine (KHM; prescribed herbal medicine by doctors of traditional Korean medicine) on liver function. Three hundred eighty four outpatients who took KHM for various conditions were enrolled for multi-center, prospective observational study. Of them 237 patients completed questionnaire and were checked liver function (aspartic aminotransferase;AST, alanine aminotransferase;ALT, alkaline phosphatase;ALP, total bilirubin;t-Bil, direct bilirubin;d-Bil, gamma glutamyltranspeptidase;GGT, protein and albumin) before and after taking KHM (duration; 21.4${\pm}$10.0 days), and data were analysed statistically. Of the 213 patients showing normal liver function test (LFT) at baseline, 209 (98.1%) remained within the normal range at the second test while 3 (1.4%) revealed slight increase of LFT. Only one subject had raised LFT regarding level of liver injury without perceived symptoms. Twenty-four of 237 patients were abnormal at baseline, and 16 at the second testing. Of the patients taking KHM, only 4 changed from normal to abnormal while 12 from abnormal to normal in their LFT. There were no significant increase in LFT level between the first and second test, except in the t-Bil level, however, the change of t-Bil was small and within normal range. The current study showed that the use of KHM did not increase the frequency of abnormal LFTs, at least in the short term.

• 치위생과학회지
• /
• 제13권3호
• /
• pp.296-303
• /
• 2013

LOX는 결합조직의 세포외기질에 존재하여 교원섬유와 탄성섬유의 교차결합을 촉진시키는 핵심적인 효소이다. 뼈세포에서 세포분화 및 세포이동과 관련된 LOX의 역할은 보고되었으나, 상아모세포 분화에서 LOX에 대한 직접적인 효과는 거의 알려지지 않았다. 이 연구에서는 인간치수세포에서 상아모세의 분화과정 동안 LOX의 역할에 대해 실험하였다. 치수세포에 저 혈청 분화유도 배지를 처리하여 0, 1, 3, 7, 14일 동안 배양하였다. 세포성장은 세포계수, 분화와 관련된 유전자들은 RT-PCR, 광물화는 alrizarin red S 염색으로 각각 실험하였다. LOX 유전자 발현은 RT-PCR로 측정하였고, LOX 효소활성은 고감도 형광분석을 시행하였다. 1. 세포성장은 저 혈청 분화유도 배지에서 시간 의존적으로 증가하였다. 2. 분화표지자인 ALP, OPN, OCN, DMP1, DSPP는 시간 의존적으로 발현되었으나 초기, 중기, 말기에 대한 차별화는 이루어지지 않았다. 3. 광물화는 3일부터 관찰되기 시작하여 14일까지 증가되었다. 4. LOX와 LOXL은 mRNA 발현이 7일까지 증가되었고, 14일에서 큰 감소를 보였다. 하지만, LOXL3와 LOXL4 mRNA는 낮은 발현을 보였고, LOXL2 mRNA는 발현되지않았다. 5. Collagen type I mRNA는 7일까지 증가하다가 14일에서 의미있게 감소를 보였고, collagen type IV는 낮은 mRNA 발현을 보였다. 6. LOX 효소 활성은 분화유도기간 중 7일에서 가장 많은 발현을 보였고, 교원질 1형 기질이 교원질 IV형 기질보다 두드러진 발현을 보였다. 이상의 결과를 종합해 볼 때, LOX isoform의 발현과 LOX 효소 활성은 인간치수세포에서 상아모세포 분화과정 동안 중요한 조절자로 사료된다.

• 대한치과보철학회지
• /
• 제43권6호
• /
• pp.751-763
• /
• 2005

Statement of problem. To improve a direct implant fixation to the bone, various strategies have been developed focusing on the surface of materials. The surface quality of the implant depends on the chemical, physical, mechanical and topographical properties of the surface. The different properties will interact with each other and a change in thickness of the oxide layer may also result in a change in surface energy, the surface topography and surface, chemical composition. However, there is limited the comprehensive study with regard to changed surface and biologic behavior of osteoblast by anodization. Purpose of study. The aim of this study was to analyze the characteristics of an oxide layer formed and to evaluate the cellular biologic behaviors on titanium by anodic oxidation (anodization) by cellular proliferation, differentiation, ECM formation and gene expression. And the phospholipase activity was measured on the anodized surface as preliminary study to understand how surface properties of Ti implant are transduced into downstream cellular events. Methods and Materials. The surface of a commercially pure titanium(Grade 2) was modified by anodic oxidation. The group 1 samples had a machined surface and other three experimental specimens were anodized under a constant voltage of 270 V(Group 2), 350 V(Group 3), and 450 V(Group 4). The specimen characteristics were inspected using the following five categories; the surface morphology, the surface roughness, the thickness of oxide layer, the crystallinity, and the chemical composition of the oxide layer. Cell numbers were taken as a marker for cell proliferation. While the expression of alkaline phosphatase and Runx2 (Cbfa1) was used as early differentiation marker for osteoblast. The type I collagen production was determined, which constitutes the main structural protein of the extracellular matrix. Phospholipase $A_2$ and D activity were detected. Results. (1) The anodized titanium had a porous oxide layer, and there was increase in both the size and number of pores with increasing anodizing voltage. (2) With increasing voltage, the surface roughness and thickness of the oxide film increased significantly (p<0.01), the $TiO_2$phase changed from anatase to rutile. During the anodic oxidization, Ca and P ions were more incorporated into the oxide layer. (3) The in vitro cell responses of the specimen were also dependant on the oxidation conditions. With increasing voltage, the ALP activity, type I collagen production, and Cbfa 1 gene expression increased significantly (p<0.01), while the cell proliferation decreased. (4) In preliminary study on the relation of surface property and phospholipase, PLD activity was increased but $PLA_2$ activity did not changed according to applied voltage. Conclusion. The anodized titanium shows improved surface characteristics than the machined titanium. The surface properties acquired by anodization appear to give rise more mature osteoblast characteristics and might result in increased bone growth, and contribute to the achievement of a tight fixation. The precise mechanism of surface property signaling is not known, may be related to phospholipase D.

• 동국한의학연구소논문집
• /
• 제1권
• /
• pp.237-249
• /
• 1992

In order to study tile effects of moxibustion on renal failure induced by $HgCl_2$ in rats aminal experiments were carry out. The moxibustion was treat into Sin-soo locus(VII23), We-soo locus(VII21) and Sin-soo + We-soo loci these loci were correspond to human body. Experimental group were divided into 4 groups : lontrol group, moxibustion groups treat on Sin-Soo and We-Soo, Sin-Soo + We-Soo group. The obtained results were as follows : 1. The concentration of serum sodium were inreased in all the groups, compared with those of the control group. Sin-Soo, We-Soo, Sin-Soo + We-Soo loci showed increased on the 1st and 3rd days. All three experimental groups showed a significant increased on 3rd day. 2. The concentration of serum potassium were decreased in all the group, compared with those of the control group. Sin-Soo, We-Soo showed a significant decrease on 3rd day. Sin-Soo + We-Soo showed a little decrease but do not significant decrease. 3. The levels of serum total protein were increased in all the group, compare with those of the control group. Sin-Soo, We-Soo show a significant increased or 3rd day. Sin-Soo + We-Soo do not showed a significant increased. 4. The levels of serum albumin were decreased in all the groups, compared with those of the control group. Sin-Soo, Sin-Soo + We-Soo showed a significant decrease on 3rd day. We-Soo does not showed a significant decrease. 5. The concentration of serum blood urea nitrogen were decreased in all the groups, compared with those of the control group. Sin-Soo, We-Soo, Sin-Soo + We-Soo showed a significant decrease on 3rd day. 6. The levels of serum alkaline phosphatase were decreased in all the group, compared with those of the control groups. Sin-Soo, We-Soo showed a significant decrease on 3rd day Sin-Soo + We-Soo not a significant decrease. In conclusion, the result of this study seemed to effect on the treatment for renal failure induced by $HgCl_2$.

• 한국융합학회논문지
• /
• 제8권5호
• /
• pp.79-85
• /
• 2017

본 연구는 만성 B형 바이러스성 감염 환자의 간 기능 혈액 검사 수치와 간 섬유화 스캔 검사(FibroScan(R))의 비교 분석을 통한 간 섬유화 스캔 검사의 임상적, 기기적 융합 유용성을 평가하고자 하였다. 2015년 7월 1일부터 2016년 2월 28일까지 대전시 B내과에 내원한 만성 B형 바이러스성 감염 환자 75명의 간 섬유화 스캔 검사 결과와 간 기능 혈액검사 수치를 분석 하고 ROC곡선을 작성하였다. 알라닌 아미노전이요소, 아스파르테이트 아미노전이요소 수치가 각각 0.572, 0.502로 섬유화 수치와 가장 높은 상관관계를 보였다(p<0.000). 감마지티피, 총 빌리루빈, 알칼리성 인산분해효소 수치도 비교적 유의한 상관관계를 보였으나 알파태아단백과 총 단백정량은 통계적으로 유의하지 않았다. 또한 알부민(-0.449)과 혈소판치(-0.373)도 섬유화 수치와 상관관계가 없었다(p<0.000). 간 섬유화 정도가 높은 등급일수록 ROC 곡선의 정확도가 증가하였으며 F4의 간 경변 단계에서 가장 큰 AUROC값을 나타냈다. 따라서 간 섬유화 스캔 검사는 만성 간질환 환자의 간 기능 수치인 알라닌 아미노전이요소, 아스파르테이트 아미노전이요소 수치와 유의한 상관관계를 보여 간의 염증 검사 및 만성 간질환 진단에 매우 유용한 것으로 판단된다.

• Toxicological Research
• /
• 제24권4호
• /
• pp.307-314
• /
• 2008

The present study was carried out to investigate the potential adverse effects of 1,3-dichloro-2-propanol on pregnant dams after maternal exposure during the gestational days (GD) 6 through 19 in Sprague-Dawley rats. The tested chemical was administered orally to pregnant rats at dose levels of 0, 10, 30, or 90 mg/kg/day. During the test period, clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights, and Caesarean section findings were examined. In the 90 mg/kg group, decreases in the body weight gain and food consumption, and increases in the weights of liver and adrenal glands were observed. Serum biochemical investigations revealed increases in aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol (CHO), triglyceride (TG), alkaline phosphatase (ALP), and bilirubin (BIL) and decreases in glucose (GLU), albumin (ALB) and total protein (TP). In the 30 mg/kg group, a decrease in the food consumption and an increase in the liver weight were observed. Serum biochemical investigation also showed increases in CHO and TG and a decrease in glucose. Since there were no signs of maternal toxicity in the 10 mg/kg group, it is considered to be the no-observed-adverse-effect level (NOAEL) of 1,3-dichloro-2-propanol. It is concluded that successive oral administration of 1,3-dichloro- 2-propanol to pregnant rats for 14 days may cause significant toxicities in body weight and liver at a dose rate ${\geq}$ 30 mg/kg/day.

• Journal of Periodontal and Implant Science
• /
• 제27권4호
• /
• pp.669-684
• /
• 1997

Polypeptide growth factors belong to a class of potent biologic mediators which regulate cell differentiation, proliferation, migration and metabolism. IGF-I is polypeptides secreted by skeletal cells and is considered as regulators of bone formation. The purpose of this study is to evaluate the effects of IGF-I on bone nodule formation and alkaline phosphatase activity of MC3T3-E1 cells. MC3T3-E1 cells were seeded at $1{\times}10^4$ cells/well, $1{\times}10^5$ cells/well in alpha-modified Eagle medium containing 10% fetal bovine serum, 10 mM ${\beta}-glycerophosphate$ and $5O{\mu}g/ml$ of ascorbic acid. Before 48 hours of indicated time, medium were changed with serum free medium. After 24 hours, 0.1, 1, 10 ng/ml IGF-I were added to the cells and cultured for 3, 7, 14, 21, 28 days. And histochemical analysis was done and ALP activity was measured and was expressed as nmol/min/mg of protein. The bone nodule formation in MC3T3-E1 cells of IGF-I was seen at 21, 28 days, but there were no difference between control group and experimental groups. The ALP activity decreased when it is compare to control 2 group except for 1 ng/ml, 10 ng/ml IGF-I of 21-day-groups and 1 ng/ml IGF-I of 28-day-groups. Dose response effects of IGF-I of ALP activity in MC3T3-E1 cells were seen the highest ALP activity at 1ng/ml until 21days and the highest ALP activity at 10 ng/ml of 28 daygroups. The peak times were seen at 7-day group, 14-day group on control group and experimental group respectively, and 1 ng/ml group was the highest ALP activity, From the above results, IGF-I was not seen notable effect on bone nodule formation and decreased ALP activity of MC3T3-E1 cells but the use of IGF-I to mediate biological stimulation of MC3T3-E1 cells shows promise for future therapeutic application.

• 한방재활의학과학회지
• /
• 제28권1호
• /
• pp.1-17
• /
• 2018

Objectives The purpose of this study was to evaluate the effect of Jeopgolsan (JGS) extract on anti-oxidant, anti-inflammatory activities in RAW 264.7 cells and on factors related with fracture healing in skull fractured rat. Methods Experimental animals were divided into four groups: normal group without any treatment (Normal), contral group were treated orally with distilled water (Control), Experimental group were treated orally with JGS at a concentration of 200 mg/kg/day (JGS 200) and Experimental group were treated orally with JGS at a concentration of 200 mg/kg/day (JGS 400). Rats in each group except the normal group were induced fractures in the skull. The 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity were measured to evaluate antioxidant activity. The production of nitric oxide (NO), $interleukin-1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) in the RAW 264.7 cells were measured to evaluate anti-inflammatory activity. The production of osteocalcin calcitonin, carboxy-terminal telepeptides of type II collagen (CTX II), transforming growth $factor-{\beta}$ ($TGF-{\beta}$), bone morphogenetic protein-2 (BMP-2), Insulin and alkaline phosphatase (ALP) in serum of rats were measured to evaluate the effects of fracture healing at 0, 2, 4, and 6th week. X-rays were taken every 3 week from 0 to 6th week to evaluate fracture healing effect. Results 1. No cytotoxicity was observed. 2. DPPH and ABTS radical scavenging activity were increased in a concentration dependent manner, indicating anti-oxidant effect. 3. NO, $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$ were not significantly changed, indicating no anti-inflammatory effect. 4. Osteocalcin, Calcitonin, $TGF-{\beta}$ and ALP were significantly increased in the experimental groups. 5. CTX II, insulin were significantly decreased in the expermental groups. 6. Radiologic examination showed that union of fracture was promoted. Conclusions From above results, JGS showed significant results in factors related with fracture healing and radiologic examination. Threfore, JGS is expected to be effective in the treatment of fracture.

• 한국가축번식학회지
• /
• 제3권2호
• /
• pp.32-41
• /
• 1979

A series of experiment was conducted to determine the concentrations of progesterone and estradiol and metabolite contents in the serum of 26 Korean native cows raised at Alpine Experiment Station during the period of late pregnancy. Blood samples were collected by jugular puncture from individual cow at 5 day intervals from 30 days prepartum to 5 days prepartum and daily collected from 5 days prepartum to the day of parturition. Progesterone and estradiol concentrations in the serum were analyzed by Radioimmunoassay (R.I.A) method and serum metabolite contents were analyzed by autoanalzer MT II system. The following are summary of the results obtained: 1. Progresterone concentrations during the late preganacy were maintained at high level (5.12-11.70ng/$m\ell$) from 30 days prepartum to 8 days prepartum and fell rapidly from 5.12$\pm$1.07ng/$m\ell$ at 2 dyas prepartum to 1.48$\pm$0.32ng/$m\ell$ at 24 hrs prepartum. 2. Estradiol levles during the late pregancy increased gradually from 33.76$\pm$13.64pg/$m\ell$ at 30 days prepartum to 92.15$\pm$11.91pg/$m\ell$ at 11-15 days prepartum and increased thereafter sharply to a ranges of 161.76-238.4pg/$m\ell$ and were maintained at this increased levle until 24 hrs prepartum and decreased to 91.40pg/$m\ell$ at the parturition. 3. The correlation coefficients were found to be 0.2440 for cholesterol-progesterone relationship and -0.2552 for cholesterol-estradiol relationship, but there were statistically insignificant. 4. The changes in total protein contents during the late pregnancy were similar patterns to those of globulin and were maintained at high level only from 15 days to 5 days prepartum. 5. Glutamic oxaloacetate transaminase (GOT) levels were increased from 59.80$\pm$3.56u/$\ell$ at 90 days prepartum to 93.32$\pm$7.27u/$\ell$ at the day of parturition, but alkaline phosphatase levels were remained steady. 6. The levels of blood urea nitrogen, glucose and calcium remained almost constant during the late pregnancy. However, glucose concentration increased around the time of parturition.