• Title/Summary/Keyword: Protein digestion

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In vitro Digestibility and Sensory Properties of different Bap(Cooked Rice) (밥의 종류에 따른 in vitro 분해율 및 관능적 특성)

  • Kim, Yoon-Sun;Lee, Gui-Chu
    • Journal of the Korean Society of Food Culture
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    • v.22 no.6
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    • pp.820-826
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    • 2007
  • Different types of bap(cooked rice) was cooked using barley or/and SoRiTae with rice as the base. Total(TS), rapidly digestible(RDS), slowly digestible(SDS) and resistant(RS) starch fractions were determined. Other physicochemical properties such as moisture, protein, amylose contents, protein digestion in vitro and color values as well as sensory properties of different bap were also investigated. Cooked rice with SoRiTae(RiSo) showed the highest moisture content of 63.9%, whereas other bap showed simillar content ranging from 62.3-63.0%. Crude protein content of RiSo was the highest, while that of cooked rice(Ri) was the lowest(p<0.05). Amylose content of RiBa was the highest, while that of RiSo was the lowest(p<0.05). In in vitro protein digestibility(IVPD), cooked rice with barley and SoRiTae(RiBaSo) was the highest, while Ri was the lowest, showing no significant difference at p<0.05. In starch fractions, as barley or/and SoRiTae were added to rice, a decrease in RDS content and increases in SDS and RS contents were observed. In addition, starch digestion index(SDI), which derived as an indicator of their in vitro starch digestibility and rapidly available glucose(RAG) value, which determined as a predictor of potential glycemic response decreased. A decrease in L value from RiSo and RiBaSo, which comprised of SoRiTae and increases in a and b values in RiSo and RiBa were observed, respectively. All sensory parameters involving color, glossiness, sweet taste, wetness, roughness, hardness and stickiness were shown to be a significant difference except sweet taste among different bap(p<0.05). L value of instrumental characteristic was negatively correlated with color of sensory characteristic and a value was positively correlated. Significant negative correlation was found between RS content and glossiness, however, positive correlation with roughness and hardness, respectively. These results suggested that cooked rice mixed with barley and SoRiTae contain significant RS and SDS contents and may improve diabetes and hyperlipidemia, due to the lowering RDS and RAG, respectively.

Ruminal and Intestinal Digestibility of Some Tropical Legume Forages

  • Khamseekhiew, B.;Liang, J.B.;Wong, C.C.;Jalan, Z.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.3
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    • pp.321-325
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    • 2001
  • Two experiments were conducted to examine the degradation rates of 4 tropical legume forages in rumen and intestine of Kedah-Kelantan (KK) cattle. Three KK cattle, averaging $173{\pm}17.15kg$ each fitted with a permanent ruminal and a T-shaped duodenal cannulae were used. The cattle were fed a maintenance diet (1% DM of their body weight) composing of 60% oil palm frond (OPF) pellet and 40% of a legume mixture of Arachis pintoi (AP) and Leucaena throughout the study. The overall DM and CP degradabilities in the rumen for Gliricidia sepium (GS) and AP were significantly higher than those for Leucaena leucocephala-Bahru (LB) and Leucaena leucocephala-Rendang (LR). This implies that LB and LR would have higher dietary protein flows into the intestine for the more efficient enzymatic digestion. However, the results of the present study suggested only limited proportions of the ruminal undegraded protein in the Leucaenas were digested in the intestine.

Association of a Single Nucleotide Polymorphism in the 5'-Flanking Region of Porcine HSP70.2 with Backfat Thickness in Duroc Breed

  • Chen, Ming-Yu;Huang, San-Yuan;Lin, En-Chung;Hseu, Tzong-Hsiung;Lee, Wen-Chuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.1
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    • pp.100-103
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    • 2003
  • Higher environmental temperature affects the economic performance of pigs. Heat shock protein 70 has been shown to play an important role in thermoresistance. The purpose of this study was to assess the effect of a single nucleotide polymorphism in the 5'-flanking region of porcine HSP70.2 on growth performance in Taiwanese Duroc. The genotype of this nt 393 polymorphic site could be verified by digestion with Bsa WI restriction enzyme of a PCR product. Pigs with TT and TC genotypes have thinner backfats than those with CC type (p<0.05). The result suggested that the polymorphic Bsa WI site in the 5'flanking region of porcine HSP70.2 may be used as a marker for the early selection of ultrasonic backfat thickness in Duroc pigs.

Protoplast Reversion of Trichoderma koningii (Trichoderma koningii의 Protoplast Reversion에 대하여)

  • 조남진;박희문;이영하
    • Korean Journal of Microbiology
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    • v.19 no.4
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    • pp.192-198
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    • 1981
  • Protoplasts from Trichoderma koningii were fractioned at varing digestion periods. There were distinct differences between the fractionated protoplasts in CMCase activity and protein content ; namely, protein contents and CMCase activities in the early-protoplasts were higher than the late-produced protoplasts. Reversion frequencies of the early-produced protoplasts. Reversion frequencies of the early-produced protoplasts (0-1hr.) and the late-produced protoplasts (1-2.5 hr.)were 1.25% and 0.56%, respectively. From these results it was assumed that the early-produced protoplasts were more active in physiological metabolism. Meanwhile, in osmotically stabilized liquid medium two distinct reversion patterns of these protoplasts were found. In the first, normal germ tube was developed from the opposite side of the protoplast after the production of an aberrent tube. In the second, reversion occurred through germination of protoplast, but this patterns was uncommon. It is suggested that the the physiological and morphological heterogeneities in protoplast reversion are related to the heterogeneous origins of protoplasts from highly differentiated mycelium.

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Characteristics of Food Waste Leachate Derived from Feed Supplement- and Compost-Producing Facilities (사료화 및 퇴비화 공정 유래 음폐수의 성상 비교 연구)

  • Shin, Seung Gu;Han, Gyuseong;Bae, Young-shin;Hwang, Seokhwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.23 no.3
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    • pp.68-77
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    • 2015
  • This study was conducted to characterize food waste leachate (FWL) and to compare its characteristics according to generation source: feed supplement- and compost-producing facilities. FWL contained high levels of organic compounds such as carbohydrate, protein and lipid. The moisture content of FWL was among the range of conventional wet anaerobic digestion. FWL was acidic (pH of $4.0{\pm}0.3$) and showed high coefficients of variance for specific parameters: carbohydrate, protein, lipid, ethanol, acetic acid and propionic acid. FWL derived from feed supplement-producing facilities showed slightly lower concentrations of most parameters than FWL derived from compost-producers. However, the difference was not significant at 5% significance level according to analysis of variance.

Comparison of forage yield and growth characteristic of two forage rice cultivars (cv. Mogyang and cv. Mogwoo) in a reclaimed rice field

  • Choi, Bumsik;Jang, Yeongmi;Lee, Su-Hwan;Chung, Nam-Jin;Cho, Jin-Woong
    • Korean Journal of Agricultural Science
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    • v.46 no.4
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    • pp.791-798
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    • 2019
  • This experiment was conducted to find out the difference between roughage productivity and feed value by using two domestic forage rice cultivars in a mid-west reclaimed rice field in Korea. During the growing period, Na+ content in soil showed no significant difference between the two cultivars; however, the K+ content in the soil was significantly lower than that in which the Mogwo was grown. The tiller number was 45.5 to 58.8, which was higher than that of Mogyeong, and the leaf number was higher than that of Mogyeong. The dry matter yield per 10 a was 1,338 kg for Moggyang and 1,532 kg for Mogwoo. At the harvest time, the crude protein yield per 10 a produced 108 kg of Mogyang and 170 kg of Mogwoo, and the total digestion nutrient (TDN) yield per 10 a also produced about 617 kg of Mogyang and 880 kg of Mogwoo. To sum up the above results, Mogwo, which has many tiller numbers and leaf numbers, has a high dry matter production, a relatively low neutral detergent fiber (NDF) and acidic detergent fiber (ADF), and a high crude protein yield and TDN yield, and it was better when cultivating the forage rice cultivars in reclaimed land.

Influence of the Novel Urease Inhibitor Hydroquinone on Growing Lamb Nitrogen Utilization

  • Zhang, Y.G.;Shan, A.S.;Bao, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.7
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    • pp.992-997
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    • 2002
  • Two in vivo experiments were conducted to evaluate the effect of novel urease inhibitor hydroquinone (HQ) on ammonia release rate from urea hydrolysis, nitrogen balance, nutrient digestibility and efficiency of microbial protein synthesis. In Exp. 1, twelve crossbred cannulated lambs were randomly assigned within initial body weight block to one of four HQ treatments, which included 0 (control), 30, 60 or 80 mg HQ/kg DM intake. Ammonia concentration and pH of ruminal fluid were immediately measured at 0, 2, 4, 6 and 8 h after feeding. Increasing the dose of HQ tended (p<0.15) to linearly decrease NH3 formation. The ammonia peak concentration (2 h post-feeding) in animals receiving HQ was approximately one-half of that in animals not receiving HQ (p<0.01), and a relatively sustained ammonia release could be obtained at the dose of 30 or 60 mg HQ/kg DM. In Exp. 2, sixteen intact crossbred lambs (weight $40{\pm}0.8kg$) were used in a $2{\times}2$ factorial design experiment. The four rations consisting of soybean meal-based (SBM) or urea-based (Urea) nitrogen source with or without HQ (S1, S0, U1 and U0) were fed in digestion and N balance trials. Apparent digestibility of major nutrients except that of ADF was not affected by either nitrogen source or addition of HQ. Regardless of nitrogen source, supplementation of HQ significantly improved ADF digestibility (p<0.05). The various ration had no effects on N metabolism in the presence of HQ. There was significant difference between total purine derivatives (PD), estimated efficiency of microbial N synthesis (p<0.05) and urea-N excretion (p<0.01) in the urine for the SBM ration and for the Urea ration. However, HQ had little influence on efficiency of microbial N synthesis as proportion of daily intake of total tract digestible OM (p>0.05). No interactions between main nitrogen source and HQ were measured throughout the trial. Results of this study suggest that addition of HQ to ration may improve ADF digestion with having no negative effect on N metabolism and microbial protein production.

DIGESTION OF ALKALI-TREATED ALFALFA SILAGE BY GOATS

  • Nishino, N.;Ohshima, M.;Miyase, K.;Yokota, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.1
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    • pp.5-11
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    • 1993
  • First crop of alfalfa (Medicago sativa L.) was harvested, wilted and ensiled with or without NaOH or $NH_3$, and fed to three rumen fistulated goats in a $3{\times}3$ Latin-square design. Each alkali treatment (2.44% of alfalfa dry matter) was made by spraying its solution prior to ensiling. Silage pH, $NH_3-N$ and butyric acid concentration were increased with each alkali addition, and NaOH-treated silage showed the lowest chemical quality. Compared with untreated silage, digestibilities of organic matter, ADF and cellulose were depressed by both alkali treatments, and the reductions in NaOH-treated silage were significant. Crude protein digestibility was also significantly decreased in NaOH-treated silage, but the goats receiving the silage excreted less nitrogen in urine than those on the other two silages. Nitrogen retention of goats was not different among the treatments. Ruminal solubility and potential degradability of dry matter and nitrogen determined with the in situ bag technique were reduced, and rate of degradation of the two components were increased by the NaOH treatment. Addition of $NH_3$ provided ruminal soluble nitrogen to the silage, but the rate of degradation was similar to that of untreated silage. These results suggest that NaOH treatment would denature the protein and reduce the susceptibility to microbial degradation in the rumen, while no positive effect of alkali treatment on fiber digestion and nitrogen utilization was observed in this study.

Effect of feeding a by-product feed-based silage on nutrients intake, apparent digestibility, and nitrogen balance in sheep

  • Seok, J.S.;Kim, Y.I.;Lee, Y.H.;Choi, D.Y.;Kwak, W.S.
    • Journal of Animal Science and Technology
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    • v.58 no.2
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    • pp.9.1-9.5
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    • 2016
  • Background: Literature is lacking on the effects of feeding by-product feed (BF)-based silage on rumen fermentation parameters, nutrient digestion and nitrogen (N) retention in sheep. Therefore, this study was conducted to determine the effect of replacing rye straw with BF-based silage as a roughage source on ruminal parameters, total-tract apparent nutrient digestibility, and N balance in sheep. Methods: The by-product feed silage was composed of spent mushroom substrate (SMS) (45 %), recycled poultry bedding (RPB) (21 %), rye straw (11 %), rice bran (10.8 %), corn taffy residue (10 %), protected fat (1.0 %), bentonite (0.6 %), and mixed microbial additive (0.6 %). Six sheep were assigned randomly to either the control (concentrate mix + rye straw) or a treatment diet (concentrate mix + BF-based silage). Results: Compared with the control diet, feeding a BF-based silage diet resulted in similar ruminal characteristics (pH, acetate, propionate, and butyrate concentrations, and acetate: propionate ratio), higher (p < 0.05) ruminal NH3-N, higher (p < 0.05) ether extract digestibility, similar crude protein digestibility, lower (p < 0.05) dry matter, fiber, and crude ash digestibilities, and higher (p < 0.05) N retention (g/d) Conclusion: The BF-based silage showed similar energy value, higher protein metabolism and utilization, and lower fiber digestion in sheep compared to the control diet containing rye straw.

The Requirement of Ruminal Degradable Protein for Non-Structural Carbohydrate-Fermenting Microbes and Its Reaction with Dilution Rate in Continuous Culture

  • Meng, Q.X.;Xia, Z.G.;Kerley, M.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.10
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    • pp.1399-1406
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    • 2000
  • A continuous culture study was conducted to determine the impact of ruminal degradable soy protein (S-RDP) level and dilution rate (D) on growth of ruminal non-structural carbohydrate-fermenting microbes. Corn starch, urea and isolated soy protein (ISP) were used to formulate three diets with S-RDP levels of 0, 35 and 70% of total dietary CP. Two Ds were 0.03 and $0.06h^{-1}$ of the fermenter volume in a single-effluent continuous culture system. As S-RDP levels increased, digestibilities of dietary dry matter (DM), organic matter (OM) and crude protein (CP) linearly (p=0.001) decreased, whereas digestion of dietary starch linearly (p=0.001) increased. Increasing D from 0.03 to $0.06h^{-1}$ resulted in decreased digestibilities of dietary DM and OM, but had no effect on digestibilities of dietary starch (p=0.77) and CP (p=0.103). Fermenter pH, the concentration of volatile fatty acids (VFA) and daily VFA production were unaffected (p=0.159-0.517) by S-RDP levels. Molar percentages of acetate, propionate and butyrate were greatly affected by S-RDP levels (p=0.016-0.091), but unaffected by D (p=0.331-0.442). With increasing S-RDP levels and D, daily bacterial counts, daily microbial N production (DMNP) and microbial efficiency (MOEFF; grams of microbial N produced per kilogram of OM truly digested) were enhanced (p=0.001). The increased microbial efficiency with increasing S-RDP levels is probably the result of peptides or amino acids that served as a stimulus for optimal protein synthesis. The quantity of ruminal degradable protein from soy proteins required for optimum protein synthesis of non-structural carbohydrate-fermenting microbes appears to be equivalent to 9.5% of dietary fermented OM.