• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1355-1359
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• 2003

This study examined the replacement of the fish meal (FM) with commercial poultry by-product meal (PBM) in practical diets for rainbow trout (Onchorynchus mykiss, Walbaum). Five isocaloric and isonitrogenous diets containing levels 0 (control), 10, 20, 30 and 40% of PBM as a replacement for FM were fed to three replicate groups of rainbow trout with a initial weight $34.50{\pm}0.43g$ (mean${\pm}$SE). Protein efficiency ratio (PER) of diets containing PBM up to 20% were similar to the control while significantly lower values were obtained from the groups receiving higher levels of PBM (p<0.05). Apparent protein digestibility coefficients (ADCs) were significantly lower than that of the control group when PBM was included at level of 20% or more. Similarly, significantly lower values were observed with diets containing 30 and 40% PBM in terms of dry matter, organic matter, ether extract, ash and energy digestibility (p<0.05). Dietary treatments did not significantly affect the body composition of the fish in terms of dry matter, crude protein, ether extract and ash. In conclusion, PBM in a proportion of 20% may replace about 40 % of FM in rainbow trout diet without significant impairment grow.

• Journal of Microbiology and Biotechnology
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• 제33권4호
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• pp.511-518
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• 2023

The use of dietary protein products has increased with interests in health promotion, and demand for sports supplements. Among various protein sources, milk protein is one of the most widely employed, given its economic and nutritional advantages. However, recent studies have revealed that milk protein undergoes fecal excretion without complete hydrolysis in the intestines. To increase protein digestibility, heating and drying were implemented; however, these methods reduce protein quality by causing denaturation, aggregation, and chemical modification of amino acids. In the present study, we observed that Lacticaseibacillus rhamnosus IDCC 3201 actively secretes proteases that hydrolyze milk proteins. Furthermore, we showed that co-administration of milk proteins and L. rhamnosus IDCC 3201 increased the digestibility and plasma concentrations of amino acids in a high-protein diet mouse model. Thus, food supplementation of L. rhamnosus IDCC 3201 can be an alternative strategy to increase the digestibility of proteins.

• Asian-Australasian Journal of Animal Sciences
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• 제30권1호
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• pp.57-63
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• 2017

Objective: Two experiments were conducted to determine the effects of adding exogenous phytase and xylanase, individually or in combination, as well as pelleting on nutrient digestibility, available energy content of wheat and the performance of growing pigs fed wheat-based diets. Methods: In Experiment 1, forty-eight barrows with an initial body weight of $35.9{\pm}0.6kg$ were randomly assigned to a $2{\times}4$ factorial experiment with the main effects being feed form (pellet vs meal) and enzyme supplementation (none, 10,000 U/kg phytase, 4,000 U/kg xylanase or 10,000 U/kg phytase plus 4,000 U/kg xylanase). The basal diet contained 97.8% wheat. Pigs were placed in metabolic cages for a 7-d adaptation period followed by a 5-d total collection of feces and urine. Nutrient digestibility and available energy content were determined. Experiment 2 was conducted to evaluate the effects of pelleting and enzymes on performance of wheat for growing pigs. In this experiment, 180 growing pigs ($35.2{\pm}9.0kg\;BW$) were allocated to 1 of 6 treatments according to a $2{\times}3$ factorial treatment arrangement with the main effects being feed form (meal vs pellet) and enzyme supplementation (0, 2,500 or 5,000 U/kg xylanase). Results: In Experiment 1, there were no interactions between feed form and enzyme supplementation. Pelleting reduced the digestibility of acid detergent fiber (ADF) by 6.4 percentage units (p<0.01), increased the digestibility of energy by 0.6 percentage units (p<0.05), and tended to improve the digestibility of crude protein by 0.5 percentage units (p = 0.07) compared with diets in mash form. The addition of phytase improved the digestibility of phosphorus (p<0.01) and calcium (p<0.01) by 6.9 and 7.6 percentage units respectively compared with control group. Adding xylanase tended to increase the digestibility of crude protein by 1.0 percentage units (p = 0.09) and increased the digestibility of neutral detergent fiber (NDF) (p<0.01) compared with control group. Supplementation of the xylanase-phytase combination improved the digestibility of phosphorus (p<0.01) but impaired NDF digestibility (p<0.05) compared with adding xylanase alone. In Experiment 2, adding xylanase increased average daily gain (p<0.01) and linearly improved the feed:gain ratio (p<0.01) compared with control group. Conclusion: Pelleting improved energy digestibility but decreased ADF digestibility. Adding xylanase increased crude protein digestibility and pig performance. Phytase increased the apparent total tract digestibility of phosphorus and calcium. The combination of phytase-xylanase supplementation impaired the effects of xylanase on NDF digestibility.

• Asian-Australasian Journal of Animal Sciences
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• 제21권2호
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• pp.232-236
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• 2008

This experiment was performed to investigate the effects of two energy levels and four lysine:digestible energy (DE) ratios on the apparent digestibility of nutrients in finishing pigs. The experiment was conducted using a $2{\times}4$ randomized complete block (RCB) design with three replicates. Twenty-four cross-bred finishing barrows ((Landrace${\times}$Yorkshire)${\times}$Duroc) with an average body weight of $64.2{\pm}0.69kg$ were assigned to one of eight treatments. Each barrow was placed in an individual metabolism crate and dietary treatment and water was provided ad libitum. Diets were designed to contain lysine:ME ratios of 1.5, 1.8, 2.1 and 2.4 g/Mcal at 3.35 and 3.6 Mcal/kg of diet in a $4{\times}2$ factorial arrangement. Dry matter (DM), ash, Ca and P digestibility were not affected by energy density or lysine:DE ratios. Crude fat digestibility increased as the energy density increased from 3.35 to 3.6 Mcal of DE/kg. Increasing the lysine:DE ratio also increased crude protein digestibility. There were no interactions between energy density and lysine:DE ratio in terms of nutrient digestibility. Nitrogen excretion via feces was not affected by energy density and lysine:DE ratio, while nitrogen excretion via urine was significantly affected by energy density and lysine:DE ratio. The apparent digestibility of all amino acids except for isoluecine, arginine and aspartic acid as well as average values of essential amino (EAA), non-essential amino acids (NEAA) and total amino acid digestibility (p>0.05) were not affected by energy density. The apparent digestibility of all amino acids except for leucine, proline, alanine and tyrosine, NEAA and total amino acid digestibility were significantly affected by lysine: DE ratio (p<0.05). Interactive effects of energy and lysine:DE ratio also significantly affected amino acid digestibility except for isoleucine, alanine, cystine, leucine, phenylalanine, glutamine and proline (p<0.05). In conclusion, these results suggest that maintaining the appropriate lysine:DE ratio becomes more important as the energy density of the diet increases. Consequently, increasing the lysine:DE ratio can result in increased crude protein digestibility and urinary nitrogen excretion, although apparent protein digestibility and nitrogen excretion were not affected by energy density Furthermore, increasing the lysine:DE ratio also increased the apparent digestibility of essential amino acids, except for leucine, regardless of energy density. The optimum lysine:DE ratio for maximum essential amino acid digestibility of the $64.2{\pm}0.69kg$ pig is approximately 2.4 g of lysine/Mcal of DE.

• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1167-1173
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• 2009

This study was conducted to evaluate the effects of providing fermented soy protein to weaned pigs on nitrogen balance and apparent fecal and apparent ileal digestibility (AID) of AA. Four weaned ((Yorkshire${\times}$Landrace)${\times}$Duroc) barrows (BW = 6.58${\pm}$0.98 kg), surgically fitted with a simple T-cannula approximately 15 cm prior to the ileo-cecal junction, were fed four diets according to 4${\times}$4 Latin square design. Diets were a basal diet supplemented with one of the following: 3% SDPP (spray dried plasma protein), 5% RBP (soy protein fermented by Lactobacillus spp.), 5% PSP (soy protein fermented by Aspergillus oryzae and Bacillus subtilis), and 2.5% RPP (2.5% RBP+2.5% PSP). No differences were observed in DM and N intakes among treatments. However, the level of urine excretion was greater in the RPP group than in the PSP group. Additionally, fecal DM excretion, fecal N concentration and fecal N excretion were increased in the RBP, PSP and RPP groups when compared with the SDPP group (p<0.05). Furthermore, total excretion was increased in the RPP group when compared with the PSP group (p<0.05). In addition, N absorption and the N absorption ratio were higher in the SDPP group than in the RPP group (p<0.05). Moreover, the DM and N digestibilities were lower in the RBP, PSP and RPP groups than in the SDPP group (p<0.05), and the ash and energy digestibilities were higher in the SDPP and RBP groups than in the PSP and RPP groups (p<0.05). However, no significant differences were observed in the DM, N, Ash, Ca, P or ileal digestibilities among treatments, although the energy digestibility was higher in the SDPP group than the RBP group (p<0.05). In addition, the apparent ileal digestibilities of essential amino acids (Arg, His, Iso, Leu, Lys, Phe, Thr, and Val) were significantly higher in the SDPP group than in the other groups (p<0.05), and the levels of Ala, Cys, Glu and Try were greater in the SDPP treatment group than the RBP, PSP and RPP groups (p<0.05). Additionally, the levels of Asp, Gly and Ser were higher in the SDPP group than the PSP and RPP groups, and the level of Pro was higher in the SDPP group than the RPP group (p<0.05). Finally, total non-essential amino acid and total amino acid digestibility were higher in the SDPP group than in the other treatments (p<0.05). Taken together, the results of this study indicate that animal protein is more bioavailable than plant protein. However, the N absorption ratio and ileal digestibility were found to be similar in the SDPP and RBP groups.

• Asian-Australasian Journal of Animal Sciences
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• 제20권12호
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• pp.1874-1879
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• 2007

A total of one hundred and forty four weaned pigs with an average BW of $8.09{\pm}0.05$ kg were used in a 28 day study to investigate the effects of fermented soy protein on growth performance, blood urea nitrogen and total protein concentrations in serum and nutrient digestibility in weaner pigs. Pigs were blocked by initial body weight and randomly allocated to one of four dietary treatments in a randomized complete block design. There were six replications per treatment. Dietary treatments included: SBM (corn-soybean meal basal diet), F 5, 10 and 15 (fermented soy product was used at 5, 10 and 15% to replace soybean meal in basal diet, respectively). ADG (average daily gain) and ADFI (average daily feed intake) were not affected (p>0.05) by dietary treatments during the entire 4-wk study period. There were linear increments in feed efficiency (p<0.01) as the dietary FSP level increased during the entire feeding period. No significant differences were observed for dry matter and nitrogen digestibility during the experimental period (p>0.05). Digestibilities of histidine, lysine and methionine were increased as the FSP level increased (linear effect, p<0.05, p<0.01). Among non-essential amino acids, alanine, glutamic acid, serine, tyrosine and total non essential amino acid digestibilities were increased linearly (p<0.05, p<0.01). There were quadratic effects in protein digestibility (p<0.05). Total amino acid digestibility of the F15 diet was improved compared with the F5 diet (p<0.05). There were no significant differences in fecal consistency score among the treatments (p>0.05). At the end of experiment, BUN (blood urea nitrogen) concentration was increased as the FSP level increased (linear effect, p<0.01) and total protein concentration was lowest (p<0.05) for pigs fed the SBM diet among treatments. In conclusion, the feeding of 10 or 15% FSP to nursery pigs improved feed efficiency, amino acid digestibility and blood urea nitrogen and total protein concentrations in blood.

• Journal of Nutrition and Health
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• 제35권8호
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• pp.848-853
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• 2002

For the Exp. 1, twelve Duroc $\times$ Yorkshire $\times$ Landrace pigs (6.12 $\pm$ 0.24 kg average initial body weight and 21 d average age) were used in an 8-d metabolic assay to determine the effects of replacing spray-dried plasma protein (SDPP) with spray-dried egg Protein containing egg yolk antibody (SDCEP) on nutrient and amino acids digestibility in weaning Pigs. Experimental animals were fed diets containing SDPP, SDCEP (spray-dried commercial egg protein) and SDAEP. Protein content in the SDPP, SDCEP and SDAEP were 58.20, 45.83 and 41.85%, respectively. Pigs fed the SDPP diet tended to increase the apparent digestibility of dry matter and nitrogen compared to pigs fed the SDAEP diets without significant differences. The ileal digestibility of lysine and methionine for the SDAEP were greater than those for the SDPP, however, there are no significant differences between groups. For Exp.2, 36 Duroc $\times$ Yorkshire $\times$ Landrace pigs (4.11 $\pm$ 0.05 kg average initial body weight and 14 d average age) were used in a 10-day growth assay to determine the effects on growth performance and nutrient digestibility of replacing SDPP with SDAEP in early-weaning pigs. Experimental animals were fed diets containing CON (corn-dried whey-SBM based diet), SDAEP3 (corn-dried whey-SBM based diet + 3% SDAEP), SDAEP6 (corn-dried whey-SBM based diet + 6% SDAEP). The average daily weight gain of the pigs fed the SDAEP3 diet was higher than that for the pigs fed the CON and SDAEP6 diets (p < 0.05). SDAEP3 significantly increased the digestibility of dry matter and nitrogen compared to the CON and SDAEP6 diets (p < 0.05).

• 한국식품과학회지
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• 제24권3호
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• pp.279-283
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• 1992

식물성 식품중의 영양 저해 인자로 알려진 phytate를 제거한 분리 유채단백(low-phytate rapeseed protein isolate)을 제조하여 pH 및 phytate 첨가량이 이들 분리 유채단백의 용해성과 소화율에 미치는 영향을 살펴보았다. 분리 유채단백의 phytate 함량은 1.5%로서 탈지 유채분말보다 66%가 감소되었으며 protein : phytate ratio는 58 : 1이었다. 분리 유채단백의 용해도는 pH 2.0과 pH 11.5에서는 매우 높았으나 pH 5.0에서는 매우 낮았다. 그 용해도에 미치는 phytate 첨가량의 영향을 보면 pH 5.0과 pH 11.5에서는 별 차이가 없었으나 pH 2.0에서는 phytate 첨가량이 많을수록 그 용해도가 감소하였다. 분리 유채단백의 pepsin 소화율에 미치는 phytate의 저해적 영향은 phytate 함량이 증가할수록 더 커졌다. 소화기간 초기에는 그 영향이 매우 컸으나 소화시간이 길어짐에 따라 그 영향은 점점 감소되었으며 평균소화율은 24%가 감소하였다. 따라서 유채단백을 식품소재로 이용하기 위해서는 phytate 함량이 낮은 제품을 제조함으로써 그의 기능성과 영양가를 향상시킬 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.234-238
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• 2003

This experiment was conducted to measure nutrient digestibility and performance in broiler chicks fed diets based on normal and low viscosity rye or barley fed with and without enzyme (pentosanase and $\beta$-glucanase) during a 17 day growth trial. A total of 150 one-day old, male broiler chicks (5 birds per pen and 5 pens per treatment) were randomly assigned to one of six dietary treatments in a $3{\times}3$ factorial design experiment (3 cereals${\times}$2 enzyme levels). Digestibility coefficients were determined using chromic oxide. Digestibility coefficients for dry matter and crude protein were significantly (p=0.0001) higher for the barley-based diets than for any of the rye-based diets. Digestibility coefficients for gross energy did not differ (p>0.05) due to cereal grain. There were no differences in the digestibility coefficients for dry matter and gross energy between chicks fed normal and low viscosity rye. However, the digestibility coefficient for crude protein was higher (p=0.01) for the low viscosity rye compared with the normal viscosity rye. Addition of enzyme to the diet significantly (p=0.0001) increased digestibility coefficients for dry matter, crude protein and energy. There were no significant differences in weight gain, feed intake or feed conversion between birds fed barley or rye or between birds fed normal or low viscosity rye. Enzyme supplementation significantly improved (p=0.0001) weight gain, intake and feed conversion. The overall results of this experiment indicate that unsupplemented barley and rye do not support adequate growth rates in poultry. Enzyme supplementation dramatically improved broiler performance. In addition, genetic selection to reduce the viscosity of rye had only a modest effect on the nutritive value of rye for broilers.

• 한국수산과학회지
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• 제15권4호
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• pp.263-270
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• 1982

해중의 영양학적인 기초자료를 얻기 위하여 multi-enzyme system을 이용한 체외소화율과 아미노산분석을 기초로 한 단백질수를 측정하였다. 김 (P. tenera)의 체외소화율은 $78.5\sim82.2$로서 산지와 건조조건에 따라 약간의 차이를 보였으며, 잎파래(E. linza)나 다른 갈조류 (미역 U. pinnatifida, 톳 H. fusiforme, 모자반 S. fuuellum)에 비하여 높았고 효소활성 저해물질 (trypsin inhibitor)의 함량은 갈조류에서 높았다. 잎과래는 채외소화율이 김보다 낮음에도 불구하고 효소활성저해물질이 가장 낮은 특이한 결과론 보였다. 전체적으로 해조류의 체의소화을이 다른 연구자들의 생체실험에 의한 소화율 (in vivo digestility)보다 높은 결과를 보인 것은multi-enzyme system을 이용한 체외소화율 측정 방법을 해조류의 정확한 소화율 측정에 적용하기에는 문제성이 있는 것으로 밝혀졌다. 일륜 김에 대한 microwave cooking의 영향은 가열시간이 15분 경과하여도 현저한 소화율 증가는 볼 수 없었으며, 효소활성 저해물질함량은 서서히 감소하는 경향을 보였다. 또한 한국식으로 구운김의 체외소화율은 microwave로 15분 가열한 시료와 비슷하였다. 아미노산 분석 결과를 이용한 단백계수(Factor method)는 김의 경우 6.52로 계산되었고, 잎파래는 6.00, 미역 6.11, 모자반 5.85, 톳은 5.83이었으며, Kjeldahl전소분석결과를 이용한 단백계수(Kjeldahl Method)는 김 6.29, 잎파래 5.83, 미역 5.40, 모자반 5.45, 톳 5.49로 나타나, 종래의 조단백계수(6.25)보다 낮은 결과를 보였다. 해조추의 비단백태질소의 정확한 규명이 없는 상태에서, 해조의 단백질함량 측정에는 아미노산 분석결과를 이용한 새로운 단백계수(Factor Method)를 사용함이 바람직한 것으로 생각되었다.