• Asian-Australasian Journal of Animal Sciences
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• v.19 no.10
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• pp.1422-1428
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• 2006

In order to investigate the effect of different levels of niacin supplementation on growth, nutrient utilization, their balance and blood biochemical profile, 15 male buffalo calves (9-10 months of age, $88.4{\pm}4.37kg$ average body weight) were divided into 3 equal groups each of 5 calves, following a completely randomized design, and fed individually for 120 days with wheat straw and concentrate mixture to meet their nutrient requirements. In addition calves were supplemented with 0 ppm (control, group I), 100 ppm (group II) and 200 ppm (group III) niacin. After 90 days of experimental feeding a metabolism trial was conducted to estimate the digestibility of nutrients and their balance. Fortnightly body weights were recorded to assess their growth rate and blood was collected from the jugular vein at day 0 and subsequently at 30-day intervals from all the experimental buffalo calves to study blood biochemical parameters. Results showed that intake and digestibility of dry matter, organic matter, crude protein, ether extract, total carbohydrates, neutral detergent fibre, acid detergent fibre, cellulose and hemicelluloses were statistically similar in the 3 groups. Buffalo calves in all three groups were in positive nitrogen, calcium and phosphorus balance, without showing any significant effect of the treatments. Dry matter, crude protein, digestible crude protein and total digestible nutrient intake ($g/d/kgW^{0.75}$) were similar in the control and niacin supplemented groups. Digestible crude protein (%) and total digestible nutrients (%) in the ration of the 3 groups were 8.07, 7.99, 7.92 and 56.70, 56.63, 56.74, respectively, and were comparable among the groups. The average daily gain (g) in-group II (567.50) was not significantly (p>0.05) higher than group I (500.0) and group III (510.0). Blood biochemical constituents (glucose, total protein, albumin, globulin, urea-N, insulin) showed no significant effect of niacin supplementation. However, serum cholesterol (mg/100 ml) was significantly (p<0.01) lower in the 200 ppm niacin-supplemented group than in the control and 100 ppm niacin-supplemented groups. It can be concluded that supplementation of niacin at 100 and 200 ppm in the diet of buffalo calves had no significant beneficial effect on their growth and nutrient utilization.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.11
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• pp.1553-1561
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• 2004

Two feeding trials were conducted with 128 pullets from 4 to 20 weeks of age and 96 laying hens from 23 to 63 weeks of age to evaluate the effects of scavenging and type of protein supplement on the feed intake and performance of improved pullets and laying hens. The experiments had a completely randomized design with four dietary treatments and four replicates. Treatments were: Control (Cont), scavenging but with access to a balanced concentrate at night; confinement (CF) and given the control feed ad libitum; scavenging and supplemented at night with the control feed, but with soybean meal replaced by cassava leaf meal (CLM); scavenging and supplemented at night with the control feed, but with fishmeal replaced y soybean meal (SBM). The mean daily dry matter (DMI), metabolizable energy (MEI) and crude protein intakes (CPI) of the pullets and laying hens, respectively, were 28%, and 18% higher for the confinement treatment (CF) compared to the scavenging treatments (p<0.001). The DMI, MEI and CPI of the pullets were not significantly different among scavenging treatments (p>0.05), but for the layers DMI, MEI and CPI were significantly higher for the CLM and SBM treatments compared to the Cont treatment (p<0.001). In the growing period, the average daily weight gain (ADG), supplement feed conversion ratio (FCR) and supplement feed cost/kg eggs (FCS) were not significantly different for CF compared to Cont, and among scavenging treatments (p>0.05). In the laying period, the hen-day production was significantly lower, and supplement FCR and FCS significantly higher for the CF compared to the scavenging treatments (p<0.001). Egg weight, and yolk, albumen and shell percentage and shape index were not significantly different among the scavenging treatments (p>0.05). However, shell and yolk percentages were significantly lower for the CF compared to the Cont treatment (p<0.01). Mortality was significantly higher for the CF compared to the scavenging treatments for pullets, and was significantly lower for the CF compared to scavenging treatments for laying hens (p<0.001). It was concluded that scavenging pullets and layers were getting around 28% and 18%, respectively, of their nutrient requirements from scavenging activities, resulting in correspondingly lower supplement feed conversion ratios and feed costs. Daily gains of the pullets were not affected by scavenging or protein supplement, but egg production and mortality were lower for the confined hens.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.8
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• pp.1118-1124
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• 2001

Fifteen lactating crossbred cows were randomly allotted to three groups of 5 each, and fed three isoproteinous concentrate mixture varying in RDP and UDP ratios, viz. 71: 29 (T1) and 58: 42 (T2) and 44: 56 (T3), along with green maize and wheat straw given as 2/3 and 1/3 of total roughage respectively, for a period of 120 days. The DM intake (kg/d) differed significantly (p<0.01) among the treatments as well as among the fortnights over a period of 120 days. DMI (kg/d) progressively increased from first to eighth fortnight in all the treatments. The daily DMI (% BW) was significantly (p<0.01) lower in T1 (2.37) than those of T2 (2.82) and T3 (2.67). The body weights of cows decreased up to 4th fortnight in T1 and up to 3rd fortnight in T2 and T3, then it showed an increasing trend till the end of the experiment in all the treatments. Cows in T1 lost 10 kg body weight but cows in T2 and T3 gained 23 and 12 kg the body weight, respectively. Both the milk and FCM yield differed significantly (p<0.01) among the fortnights. The FCM yield increased up to 2nd fortnight in all the treatments and thereafter, the FCM yield declined gradually as the lactation advanced. The FCM yield (kg/d) was significantly (p<0.05) higher in T3 (10.47) than in T2 (9.81) and T1 (9.68), however, milk yield, SCM yield and milk energy yield did not differ among the treatments as well as among the fortnights. Fat and protein % in milk increased as the lactation advanced. However, fortnightly SNF % in milk showed an irregular trend. The % fat, protein, SNF and total solids in milk differed significantly (p<0.01) among the fortnights. The % fat and protein in milk varied significantly (p<0.01) among the treatments, being lowest in T1 and highest in T3. The feed efficiency for milk production showed a non-significant variation among the treatments as well as among the fortnights, but increased with the increase in UDP level. It is concluded that by increasing the UDP level from 29 to 56 per cent of CP in the diet of medium producing cows, the milk production increases and cost of milk production reduces.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.3
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• pp.212-218
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• 1983

The effect of dietary calcium and magnesium on the 3-Hydroxy-3-methyl-glutaryl coenzyme A reductase (E.C. 1.1.1.34) in rabbit's liver microsomal protein was studied for a period of 4 weeks using isocalories and isonitro-genous as a basal diet. The experimental rabbits fed the following basal diets, such as crude protein 68.45%, carbohydrates 13.38%, fats 16.17% and added some sorts of calcium and magnesium, according to experimental plan making. The subject rabbits were divided into 9 feeding groups. The results are summarized as follows. Body weight gains per week of the groups fed magnesium and basal diet showed a little bit increase, but the groups fed calcium and basal diet showed a little bit decrease compare with control group. In case of serum magnesium, control group was 9.5mg% groups fed basal diet and magnesium were 8.27mg% in average, groups fed basal diet and calcium were 4.45mg% in average. In case of serum calcium, control group was 15.3mg%, groups fed basal diet and magnesium were 14.6mg% in average, groups fed basal diet and calcium were 14.1mg% in average. There was no great difference between magnesium fed groups in serum calcium. In serum triglyceride, control group was 82.8mg%, groups fed magnesium and basal diet were 60.3mg% in average, groups fed calcium and basal diet were 69.5mg% in average. The calcium fed groups were higher than the magnesium fed groups in serum triglyceride. In serum cholesterol, control group was 80mg%, groups fed magnesium and basal diet were 64.3mg% in average, groups fed calcium and basal diet were 56.3mg% in average. The calcium fed groups were lower than the magnesium fed groups in serum cholesterol. In case of the 3-Hydroxy-3-methylglutaryl coenzyme A reductase activity, control group was 0.998nmol/min/mg protein, groups fed magnesium and basal diet of HMG-CoA were 0.849nmol/min/mg in average.

• Korean Journal of Food Science and Technology
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• v.19 no.1
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• pp.61-65
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• 1987

The milk protein portion contains sialic acid which may be used as an index for k-casein. In comparison with non fat dry milk (NFDM) and calcium caseinate, the solubility of NFDM protein isolates at the various pH was inferior. Another consideration for the low solubility relates to the possibility that k-casein may have been extracted or possibly modified by the action of alcohol. The k-casein is the principal stabilizing fraction for casein micelle and any changes which result in its destruction or removal would be expected to have potent results. During solvent treatment, 16.5% of sialic acid was extracted in the first extraction solvent, whereas the second treatment caused only a 4.0% loss, based on sialic acid analysis. A study was conducted on the effect of concentration of methanol on loss of sialic acid. The loss of sialic acid decreased as the concentration of methanol increased to above 62% in both the first and second extracted solvent. It appears that loss of sialic acid is attributed to the water fraction rather than the methanol fraction. The effect of addition of the dried extracted solids on solubility was investigated. Protein solubility of NFDM protein isolates was sharply increased by 10% addition of the extracted solids and, thereafter, decreased. Efforts to restore solubility by feeding-back the extracted material supported the concept that removal of surface k-casein may have been a possible factor.

• Korean Journal of Food Science and Technology
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• v.7 no.3
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• pp.120-127
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• 1975

Despite the extensive study of protein effect to man and domestic animals, protein effect of sunflower seed oil cake to the growth and value of erythrocyte in rats is still remained to be investigated. This study is to investigate the effect of protein of sunflower seed oil cake to the rat growth and value of erythrocyte as well as the liver weight, heart, kidneys and spleen of rats. Sixty to seventy-days-old, male Sprague-Dawley rats weighing 50-60g, were used in this experiment and 36 rats allotted to 4 groups: standard, 30% cake, 20% cake and low protein. The standard group was fed for 7 weeks on the ration supplemented with 17% casein, 30% and 20% cake groups with sunflower seed oil cake by 30% and 20%, respectively, and low protein group without any supplementation. To observe value of erythrocyte, blood was taken from heart. Value of erythrocyte, hemoglobin and hematocrit value were determined by usual method and oxygen consumption by the method of Watts and Gourley. The result of this experiment suggests that it is recommendable to supplement deficient amino acids in animal.

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.818-837
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• 2023

Understanding adipocyte development in fetus during bovine pregnancy is important for strengthening fattening technology. Additionally, nutritional level of dams during pregnancy has the potential to improve offspring growth and fat development. The purpose of this study is to evaluate the intramuscular adipocyte development and expression level of related genes in bovine fetus, and the effect of increased crude protein (CP) intake during pregnancy on the growth performance and carcass characteristics of male offspring. Eighty six pregnant Hanwoo cows (average body weight, 551.5 ± 51.3 kg, age 5.29 ± 0.61 y) were used. Fetuses were collected at 90, 180 and 270 d of gestation from 18 pregnant Hanwoo cows. The remaining 68 pregnant cows were randomly assigned to 2 feeding groups. The control (CON) group was provided the standard protein diet (n = 34), and treatment (TRT) group was provided a diet with a 5% increase in CP intake (n = 34). Male offspring were divided into two groups according to protein treatment of the pregnant cows: CON male offspring (CON-O) and TRT male offspring (TRT-O). Intramuscular adipocytes were found in the fetal skeletal muscle after 180 days of gestation. Male calf's birth weight increased in the TRT group compared to that in the CON group (p < 0.002). The final body weight (p < 0.003) and average daily gain (p < 0.019) of male offspring were significantly higher in TRT-O than in CON-O. The feed conversion ratio was also improved by 10.5% in TRT-O compared to that in CON-O (p < 0.026). Carcass weight was significantly higher in the TRT-O group than that in the CON-O group (p < 0.003), and back fat was thicker in the TRT-O group (p = 0.07). The gross receipts and net income were higher in TRT-O than in CON-O (p < 0.04). Thus, fetal intramuscular fat can be formed from the mid-gestation period, and increased CP intake during pregnancy can increase net income by improving the growth and carcass weight of male offspring rather than intramuscular fat.

• Nutrition Research and Practice
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• v.14 no.6
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• pp.580-592
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• 2020

BACKGROUND/OBJECTIVES: The present study aimed to further investigate the potential health beneficial effects of long-term seaweed supplementation on lipid metabolism and hepatic functions in DIO mice. MATERIALS/METHODS: Four brown seaweeds (Undaria pinnatifida [UP], Laminaria japonica [LJ], Sargassum fulvellum [SF], or Hizikia fusiforme [HF]) were added to a high fat diet (HFD) at a 5% ratio and supplemented to C57BL/6N mice for 16 weeks. Triglycerides (TGs) and total cholesterol (TC) in the liver, feces, and plasma were measured. Fecal bile acid (BA) levels in feces were monitored. Hepatic insulin signaling- and lipogenesis-related proteins were evaluated by Western blot analysis. RESULTS: Fasting blood glucose levels were significantly reduced in the LJ, SF, and HF groups compared to the HFD group by the end of 16-week feeding period. Plasma TG levels and hepatic lipid accumulation were significantly reduced in all 4 seaweed supplemented groups, whereas plasma TC levels were only suppressed in the UP and HF groups compared to the HFD group. Fecal BA levels were significantly elevated by UP, LJ, and SF supplementation compared to HFD feeding only. Lastly, regarding hepatic insulin signaling-related proteins, phosphorylation of 5'-AMP-activated protein kinase was significantly up-regulated by all 4 types of seaweed, whereas phosphorylation of protein kinase B was up-regulated only in the SF and HF groups. Lipogenesis-related proteins in the liver were effectively down-regulated by HF supplementation in DIO mice. CONCLUSIONS: Brown seaweed consumption showed hypotriglyceridemic effects in the prolonged DIO mouse model. Specifically, combinatory regulation of BA excretion and lipogenesis-related proteins in the liver by seaweed supplementation contributed to the reduction of plasma and hepatic TG levels, which inhibited hyperglycemia in DIO mice. Thus, the discrepant and species-specific functions of brown seaweeds provide novel insights for the selection of future targets for therapeutic agents.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1429-1437
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• 2003

Browse tree legume leaves from Acacia spp (A. nilotica, A. tortilis, A. polyacantha), Dichrostachys sp, Flagea villosa, Piliostigma thonningii, Harrisonia sp were evaluated for nutritive potential (chemical compositions and degradability characteristics) compared to Gliricidia sepium. Effect of tannins anti-nutritive activity on digestibility was also assessed by polyethylene glycol (PEG) tannin bioassay. Crude protein (CP), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) differed (p<0.05) between legume foliages. Mean CP, ash, NDF, ADF and ADL for fodder species tested were 158, 92, 385, 145, and 100 g/kg DM, respectively. CP ranged from 115 (P. thonningii) to 205 g/kg DM (G. sepium). Acacia spp had moderate CP values (g/kg DM) of 144 (A. nilotica), to high CP in A. tortilis (188) and A. polyacantha (194) comparable to G. sepium. The forages had relatively lower fiber compositions. A. nilotica had (p<0.05) lowest NDF, ADF and ADL (182, 68 and 44) compared to P. thonningii (619, 196 and 130) g/kg DM, respectively. Except G. sepium, all fodder species had detectable high phenolic and tannin contents greater than 5% DM, an upper beneficial level in animal feeding and nutrition. Mean total phenolics (TP), total tannins (TT) and condensed tannins (CT) (or proanthocyanidins) for fodder species tested were 139, 113 and 43 mg/g DM, respectively. F. villosa had (p<0.05) lowest TP and TT of 65 and 56 mg/g DM, respectively, compared to A. nilotica (237 and 236 mg/g DM, respectively). The CT varied (p<0.05) from 6 (F. villosa) to 74 mg/g DM (Dichrostachys sp). In vitro organic matter (OM) degradability (OMD) differed (p<0.05) between fodder species. G. sepium had (p<0.05) high degradability potential compared to A. polyacantha that had (p<0.05) the lowest OMD values. Forage degradability ranked: G. sepium>A. nilotica>P. thonningi>F. villosa>Dichrostachys sp>A. tortilis>A. polyacantha. Addition of PEG resulted to (p<0.05) improvement in in vitro OM digestibility (IVD). Increase in IVD was mainly due to binding action of PEG on tannins; and represents potential nutritive values previously depressed by tannins anti-nutritive activity. Browse fodder has potential as sources of ruminal nitrogen especially for ruminants consuming low quality roughages due to high protein, lower fiber compositions and high potential digestibility. However, utilization of browse supplements in ruminants is hampered by high phenolic and tannin contents. Deactivation of tannin anti-nutritive activity, possibly by feeding tanniniferous browse with other readily available nitrogen sources to dilute tannin anti-nutritive activity could improve utilization of browse fodder supplements. Further studies are needed to assess browse fodder palatability and intake, and their effect on growth performance in ruminants.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.487-494
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• 1992

This experiment was conducted to study the effect of addition of Lucerne juice (LJ) obtained by mechanical extraction of freshly harvested crop on the nutritive value of rice straw silage. Rice straw (RS) was ensiled with intact, NaOH or $NH_3$ treated LJ at 3:7 ratio on fresh weight basis (LJ RS, LJ NaOH RS and LJ $NH_3$ RS, respectively). Each alkali was mixed with fresh juice at a level of 4% of rice straw dry matter just before ensiling. Rice straw ensiled with water was prepared as the control (W RS). In the digestion trial, goats were allocated in a $4{\times}4$ Latin-square design and fed the diet containing three parts of RS silage and one part of wheat bran (DM basis). For the goats receiving the control silage, urea was supplemented at feeding time so as to adjust the nitrogen intake except for goats on LJ $NH_3$ RS silage. Crude protein content of RS silage was increased from 5.2 to 9.1% (DM basis) by the addition of intact LJ and to about 24% by $NH_3$ treated LJ. The control W RS silage contained only trace amount of lactic acid and was dominated by acetic and butyric acid. The addition of intact LJ reduced butyric acid content and $NH_3-N/TN$ of the silage whereas the addition of alkalized LJ increased those values and shifted to a butyrate type fermentation. Nutrient digestibilities and nitrogen balance of goats were almost the same when they were fed W RS and LJ RS silage indicating the addition of intact LJ did not improve the nutritive value. The addition of alkalized LJ significantly increased the fiber digestibilities of RS silage and $NH_3$ treatment was more effective than NaOH treatment. Postprandial ruminal $NH_3-N$ and blood urea nitrogen (BUN) concentrations were decreased by feeding LJ NaOH RS silage suggesting ruminal protein synthesis was enhanced along with the increase of energy supply for supply for rumen microbes by the alkali treatment. The advantageous fiber digestibilities of LJ $NH_3$ RS silage compared with those of LJ NaOH RS silage might be attributable to a sufficient nitrogen supply for microbial fiber digestion in the rumen.