• Korean Journal of Poultry Science
• /
• v.22 no.4
• /
• pp.203-211
• /
• 1995

In order to investigate the effect of feeding live yeast on growth performance, nutrients utilization, tibia mineral deposit and intestinal microorganism changes, a growth assay was conducted with 360 broiler chicks. Treatments were four levels of yeast as 0, 0.025, 0.05 and 0.1% in 1.83% tricalcium phosphate and two levels of yeast as 0 and 0.05% in 1.15% tricalcium phosphate. The crude protein content of live yeast was 45%, and 97% of it was in the pure protein form, with 46.6% of essential amino acids and 53.4% of non-essential amino acids. Growth performance was tended to increase by feeding the yeast but there was no significant difference(P>.05). The protein digestibility was increased as the feeding level of yeast increased. However, digestibilities of fat, fiber, calcium and phosphorus were not affected by the yeast. Ash and calcium content of tibia were increased as the level of yeast increased. Total number of E. coli in small intestine was significantly decreased(P<.05) in chicks fed yeast. Total number of Lactobaci1lus was significantly increased by the yeast feeding. The changes of microorganism in cecum had the same trend with the changes of microorganism in small intestine.

• Journal of Nutrition and Health
• /
• v.22 no.4
• /
• pp.247-256
• /
• 1989

Growing male rats were allowed to have food from two dishes containing isocaloric, isoprotein 15% or 70% carbohydrate diet for 4, 8, 12 or 24 hours a day at the beginning of the dark period. Four hours of feeding were not enough to grow though the first 4 hours of intake were significantly higher than other periods. 4-hour and 8-hour feeding groups consumed significantly less carbohydrate calorie than 12-hour and ad libitum feeding groups, which hence, showed lower ratio of carbohydrate to protein in the total food consumed. The fasting brain tryptophan and serotonin levels among groups were not significantly different. It appears therefore that the manipulation of the feeding period could be valuable in suppression of carbohydrate appetitie, especially carbohydrate carving.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.5
• /
• pp.613-618
• /
• 2000

Twelve castrated male Black Bengal goats with an average live weight of 10.3 kg (8 months old) were used in a $2{\times}2$ factorial arrangement to study the effects of dietary crude protein concentration [20.3% (HP) and 16.9% (LP)] and feeding level [ad libitum and 85% of ad libitum (restricted)] on growth and carcass characteristics. Ad libitum feeding, on average, significantly (p<0.05 to p<0.01) increased daily live weight (6.27 vs -5.86 g), dry mater intake (409 vs 351 g/d), estimated gain in carcass (0.195 vs -0.200 kg) and empty body weight (0.385 vs -0.350 kg), chemically extracted fat in meat sample (6.89 vs 6.48%), depth of M. longissimus dorsi (22.1 vs 18.3 mm) and gut and caul fat (170 vs 130 g) compared with restricted feeding regime. The greater intake of dietary protein from the HP diet resulted in significantly (p<0.05 to p<0.01) greater values for depth (20.9 vs 19.5 mm) and width (32.4 vs 27.9 mm) of M. longissimus dorsi, gut and caul fat (190 vs 110 g) and also perirenal and retroperitoneal fat (85 vs 50 g) than those of the goats that received the LP diet. Similarly, the HP diet had significantly (p<0.05 to p<0.01) higher values for CP digestibility and DCP concentration than those of the LP diet. The results indicated that growth rate and carcass gain were highest in goats fed the HP diet ad libitum and therefore, diet containing 20.3% CP may be suggested for feeding growing goats.

• Journal of Life Science
• /
• v.5 no.2
• /
• pp.1-1
• /
• 1995

Casein or soybean protein was subjected to there action with caffeic acidtyrosinase system at 30-35$\circ$C, pH 6.8 with aeration for 5hr. The resulting brown proteins were washed with acetone until the washings were on longer colored. However, modified protein still retained a light brown. The effects of the modified proteins and brown compounds on male Wistar strain rats were studied by pair-feeding of a cholesterol-free diet for 14days. Significant decrease in protein digestibility for the rats fed with the modified proteins were observed. Weight gain and protein digestibility were not influenced by feeding brown compounds, but the feeding of brown compound from casein caused an enlargement of caecum. The concentrations of serum cholesterol and triglyceride in the rats fed with modified proteins and brown compounds were mostly unchanged against the rats fed with untreated proteins. These results suggest that the decrease in protein digestibility induced by enzymic browning-reaction did not cause the decrease in concentration of serum cholesterol.

• Journal of Nutrition and Health
• /
• v.11 no.3
• /
• pp.31-36
• /
• 1978

This experiment was designed to observe some influence on the serum components of growing rats maintained with basal diet supplemented with sausage. Forty male Albino rats were used as the experimental animals and the subjects were divided into four diet groups (control group; feeding of basal diet containing 10.8% and 10.4% of protein and lipid respectively, A group; feeding of sausage containing 12.5% and 27.6% of protein and lipid respectively supplemented with basal diet every day, B group; feeding of sausage supplemented with basal diet once a three-day and C group; feeding of sausage supplemented with basal diet once a five-day) and each group was fed on the corresponding diet for forty-days. The contents of protein, lipid and cholesterol in their serum were determined. The result obtained are summarized as follows: 1) The final body weight and liver weight of the A group were increased more than other groups. 2) The protein contents in the serum of the B group was increased more than other groups. 3) The total lipid contents in the serum of the A group and B group were decreased compared with control group. 4) The total cholesterol, ester form cholesterol and free form cholesterol in the serum of the A group were decreased, but the total cholesterol and ester form cholesterol in that of the C group were increased compared with control group.

• Nutrition Research and Practice
• /
• v.3 no.2
• /
• pp.95-101
• /
• 2009

It has been reported that dietary polyunsaturated fats (PUFA) increase liver injury in response to ethanol feeding. We tested the hypothesis that diets rich in linoleic acid (18:2n-6) would affect acute liver injury after acetaminophen injection and that protein restriction might exacerbate the liver injury. We examined effects of feeding diets with either 15% (wt/wt) corn oil or 14% beef tallow and 1% corn oil for six weeks with either 6 or 20 g/100 g protein on acute hepatotoxicity. After the feeding period, liver injury was induced by injecting either with 600 mg/kg body weight acetaminophen suspended in gum arabic-based vehicle, or with vehicle alone during fasting status. Samples of liver and plasma were taken for analyses of hepatic glutathione (GSH) levels and liver-specific enzymes [(Glutamate-pyruvate transaminase (GPT) and glutamate-oxaloacetate transaminase (GOT)], respectively. Whereas GSH level was significantly lower in only group fed 15% corn oil with 6 g/100 g protein among acetaminophen-treated groups, activities of GPT and GOT were significantly elevated in all groups except the one fed beef tallow with 20 g/100 g protein, suggesting low protein might exacerbate drug-induced hepatotoxicity. The feeding regimens changed the ratio of 18:2n-6 to oleic acid (18:1n-9) in total liver lipids approximately five-fold, and produced modest changes in arachidonic acid (20:4n-6). We conclude that diets with high 18:2n-6 promote acetaminophen-induced liver injury compared to diets with more saturated fatty acids (SFA). In addition, protein restriction appeared to exacerbate the liver injury.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.5
• /
• pp.665-670
• /
• 2002

The expediency of replacing cost prohibitive and often inaccessible traditional protein supplements prompted the monitoring of hematological parameters was carried out in female goats at 0, 30, 60 and 90 days post feeding. Rumen environment was (3), respectively fed supplements containing either a leaf meal mixture (LMTM) of Leucaena leucocephala-Morus alba-Tectona grandis (2:1:1) or traditional protein supplements groundnut cake (GNC) or soybean meal (SBM) and wheat straw as basal diet. The periodic monitoring of hematological parameters was carried out in female goats at 0, 30, 60 and 90 days post feeding. Rumen environment was studied in bucks in a $3{\times}3$ switch over design. Rumen liquor was collected at 0, 2, 4, 6 and 8 h post feeding after 4 weeks of feeding. The goats fed on LMTM or GNC had similar dry matter intake (g/kg $W^{0.75}$), which was significantly (p<0.05) higher than SBM. Except for packed cell volume (PCV), none of the blood biochemical constituents (Hemoglobin, serum glucose, total protein, serum albumin (A) and globulin(G), A:G ratio, alkaline phosphatase, transaminases) varied significantly due to replacement of 50% dietary protein by LMTM throughout the experiment. GNC group had significantly higher level of PCV than other treatments. However, the level of serum total protein (p<0.01) tended to increase from 60th day onwards irrespective of dietary treatments. The average rumen pH was significantly higher (p<0.001) on SBM followed by LMTM and GNC, respectively. Total volatile fatty acid (TVFA) production was comparable in goats given LMTM or GNC supplements, the corresponding values were significantly different (p<0.001) when compared with SBM. The ammonical-N, total-N and TCA-precipitable-N (mg/100 ml SRL) did not differ significantly among dietary treatments. It may be concluded that supplementing wheat straw with LMTM based concentrate had no adverse effect on voluntary intake, blood biochemical profile and rumen fermentation pattern of the goats.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.4
• /
• pp.538-546
• /
• 2008

Large-type goats eating dry forage secreted large volumes of saliva which resulted in the loss of $NaHCO_3$ from the blood and decreased plasma volume (hypovolemia). This research investigated whether or not the loss of $NaHCO_3$ from the blood and hypovolemia brought about by dry forage feeding actually depresses feed intake in large-type goats under free drinking conditions. The present experiment consisted of three treatments (NI, ASI, MI). All treatments in this experiment were carried out under free drinking conditions. In the NI control (NI), a solution was not infused. In the ASI treatment, i.v. infusion of artificial saliva was initiated 2 h before feeding and was continued for a total of 3 h concluding 1 h after the commencement of the feeding perod. In the MI treatment, mannitol solution was infused to replenish only water lost from the blood in the form of saliva. The hematocrit and plasma total protein concentrations during feeding in the NI control were observed to be higher than pre-feeding levels. This indicated that dry forage feeding-induced hypovolemia was caused by the accelerated secretion of saliva during the initial stages of feeding in freely drinking large-type goats. Increases in hematocrit and plasma total protein concentrations due to dry forage feeding were significantly suppressed by the ASI treatment. While hematocrit during feeding in the MI treatment was significantly lower than the NI control, plasma total protein concentrations were not different. From these results, it is clear that the MI treatment was less effective than the ASI treatment in mitigating the decreases in plasma volume brought about by dry forage feeding. This indicates that plasma volume increased during dry forage feeding in the ASI treatment which inhibited production of angiotensin II in the blood. The ASI treatment lessened the levels of suppression on dry forage feeding, but the MI treatment had no effect on it under free drinking conditions. The results indicate that despite the free drinking conditions, increases in saliva secretion during the initial stages of dry forage feeding in large-type goats caused $NaHCO_3$ to be lost from the blood into the rumen which in turn caused a decrease in circulating plasma volume and resulted in activation of the renin-angiotensin system and thus feeding was suppressed.

• Journal of Nutrition and Health
• /
• v.8 no.2
• /
• pp.7-13
• /
• 1975

In the classic point of view, it has well known that the tangle is the mineral source, especially iodide. However, there are few reports that the tangle can be used the high-protein source. With the consideration whether the tangle can be used as a protein source as well as one of the foodstuff or not, this studies is done by animal experiment. The Albino rats to be examined were fed on the basal diet (Table 1) and the others including the polished tangle by weight 2%, 3%, 4% and 5%, as experimental diets, for 4 weeks, respectively. The weight gain(Fig. 1), total amount of diet intake (Fig. 2), total water intake(Fig. 3), feed efficiency ratio (Fig. 4) and protein efficiency ratio (Fig. 5) was measured by each week, respectively. In addition, organ weight (Fig. 6) and blood study (Fig. 7, Fig. 8) was also analyzed at the end of the experiment. As the results of this study, growth rate, that is, weight gain, feed efficiency ratio and protein efficiency ratio in all experimental groups increased more than those of the control group. Moreover, it was also found that weight gain, feed efficiency ratio, protein efficiency ratio by feeding on the 2 percent tangle group was higher than the corresponding values of all experimental groups. However, feed intake and total water gain were the most by feeding on the 5% tangle group. This result may be interpreted to show that the total content of sodium includes more than enough in the feed intakes. To obtain further information concerning the effect of the tangle-dietary conditions on the growth rate of Albino rats, it was measured the organ weight. In the adrenal and spleen, the total weight by feeding on the all experimental groups were decreased more than that in the case of the control group. In the other hand, in the liver the weight by feeding on the high concentration groups (4%, 5% tangle diets) were not higher than the control group, while in the case of the low concentration groups (2%, 3% tangle diets) were higher than that of the control group. When it was observed the blood components, total protein, hemoglobin, sodium, potassium, white blood cell and red blood cell of rats, the content of white blood cell by feeding on all experimental groups were lower than that by feeding on the control group, but it was also clear that in the other components except white blood cell in blood the contents by feeding on all experimental groups were similar to that in the case of the control groups. However, in the 5% tangle group, the content of sodium was the lowest of all experimental groups, where as the content of potassium was the highest of all experimental groups.

• Fisheries and Aquatic Sciences
• /
• v.17 no.4
• /
• pp.449-454
• /
• 2014

The effects of food deprivation and feeding ratio on the growth, feed utilization and body composition of juvenile olive flounder Paralichthys olivaceus were investigated in 810 juvenile fish averaging 6.4 g in weight randomly distributed in 27 400-L flow-through tanks. A 3 [food deprivation: 8-week feeding without food deprivation (8W), 7-week feeding after 1-week food deprivation (7W) and 6-week feeding after 2-week food deprivation (6W)]${\time}3$ (feeding ratio: 100%, 95% and 90% of satiation, hereafter denoted by 100, 95 and 90, respectively) factorial design was applied. The weight gain of the fish was significantly affected by both food deprivation and feeding ratio. The weight gain of the fish under the 8W-100 treatment was higher than that of those under the 7W-95, 7W-90, 6W-100, 6W-95 and 6W-90 treatments, but did not differ from those of fish under the 8W-95, 8W-90 and 7W-100 treatments. The specific growth rate (SGR) and the feed intake of the fish were affected by both food deprivation and feeding ratio. However, the feed conversion ratio (FCR) was unaffected by both food deprivation and feeding ratio. The protein efficiency ratio (PER) was affected by the feeding ratio, but not by food deprivation. The protein retention (PR) was affected by food deprivation, but not by feeding ratio. Juvenile olive flounder subjected to 1-week food deprivation at 100% satiation (7W-100 treatment) achieved full compensatory growth.