• The Korean Journal of Food And Nutrition
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• v.17 no.3
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• pp.322-327
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• 2004

This study was carried out to evaluate the effect of flour's protein contents on the quality of Danish pastries made with frozen doughs. Danish pastries for frozen dough were made by straight dough method. Frozen doughs were stored for 12 weeks at -20$^{\circ}C$ and baked after thawing and fermentation a month. The volume, the moisture content, the texture and the quality evaluation of Danish pastries were investigated. The volume of Danish pastry decreased with the increase of frozen storage time. Danish pastry made by flour of 13.2% protein content showed larger volume than that made by flour of 9.2% protein content. The moisture content of Danish pastry revealed that there were no significant decrease with the increase of frozen storage time. In terms of hardness, Danish pastry made by flour of 9.2% protein content accomplished the best texture in the resulting pastry. On the quality evaluation, Danish pastry made by flour of 13.2% protein content had the highest score.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.100-100
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• 2020

Wheat is mainly composed of carbohydrate but it contains a moderate amount of protein, which gives a very useful characteristics to flour food such as the unique elasticity and stickiness of the dough. We developed a calibration equation for analyzing crude protein content using Near Infrared Spectroscopy to quick analyze the crude protein content of wheat germplasm stored in the National Agrobiodiversity Center, RDA, Korea. The 1,798 wheat germplasms were used to draw up the calibration formula. The crude protein's interval distribution of 1,798 wheat germplasms used for the calibration was 7.04-20.84%, the average content was 13.2%, and standard deviation was 2.6%. The germplasms distribution was composed of a suitable group for the preparation of the calibration formula because the content distribution was a normal, excluding the 13.0-15.5% content section. In order to verify the applicability of the NIRS prediction model, we measured the crude protein content of the 300 wheat germplasms that were not used for the calibration using both Kjeldahl analysis and NIR spectrum. The analysis value calculated using each method were statistically processed, and the test results and statistical indicators of the predictive model were compared. As a result, The R² value of the optimized NIRS prediction model was 0.997, and the Standard error of Calibration value(SEC) was 0.132, and slope value was 1.000. With prediction model selection, compared to Kjeldahl method, R² values were 0.994(Kjeldahl), 0.998(NIRS), and the SEC value were 0.191 and 0.132, respectively, comparing the statistical indices of the forecast model. And slope value were 1.013, 1.000, respectively. The analysis of crude protein content by the NIRS predictive model developed by each statistical index showing similar figures is judged to show a high degree of correlation with the Kjeldahl analysis. The proven calibration equation will be used to measure the crude protein content of wheat germplasms held by the National Agrobiodiversity Center, and by dividing the wheat germplasms by their use according to the crude protein content, it will provide useful information to relevant researchers.

• YAKHAK HOEJI
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• v.32 no.5
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• pp.313-318
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• 1988

Radioprotective ginseng protein fraction was isolated from Korean white ginseng and its cytotoxic effect on CHO-K1 cells was studied by the method of measuring the relative cell survival and total cellular protein content (FRAME method). When ginseng protein at the dose of 300, 600, 900, $1200{\mu}g/ml$ was treated to cells for 24 hrs, the relative survival was significantly decreased at the concentration of above $600{\mu}g/ml$, indicating the presence of cytotoxic effect of the protein at certain concentration. When cellular protein content was measured after ginseng protein at the dose of 300, 600, 900, $1200\;{\mu}g/ml$ was treated, the amount of cellular protein was significantly reduced at the concentration above $600{\mu}g/ml$ in the case of 24 hr treatment and at all concentrations including $300{\mu}g/ml$ in the case of 72 hr treatment. The data suggest that the protein may inhibit cell growth, resulting in the reduction of live cells in culture. $ID_{50}$ value which is the concentration of ginseng protein that reduces the total cellular protein content to 50% of the control was calculated as 2276.86 and $1323.32\;{\mu}g/ml$ in groups treated for 24 and 72 hr, respectively. Since $ID_{50}$ value of above $1000{\mu}g/ml$ indicates very weak cytotoxicity, the ginseng protein seems to exert very weak cytotoxic effect on CHO-K1 cells.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.19
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• pp.14-20
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• 1975

In order to obtain the fundamental informations on the variation of protein and amylose content in rice grain, 4 varieties were subjected to the 5 different harvesting with 5 different plantings. Amylose content in the rice grain were increased by later planting. Protein content of thermo-sensitive rice varieties were decreased while those of photo-sensitive varieties were increased by delayed planting. Interactions among planting date, variety and harvesting date were highly significant for amylose content, but not significant for protein content.

• Applied Biological Chemistry
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• v.29 no.2
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• pp.116-121
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• 1986

869 wheat lines were selected and analyzed for protein and lysine composition. Also, high protein-high lysine wheat were evaluated by stability parameter to determine varietal response to environments. Protein content had highly significant positive correlation coefficient of $0.902^{**}$ with lysine content but negatively correlated with lysine per protein content. Bezostaya and Lancota with low regression coefficient provided relatively low response to environment in protein content, while CI 13449 and Centurk with high regression coefficients (1.0) had a relatively high response. High heritability has been formed for protein $(h^2=0.809)$ and lysine content $(h^2=0.647)$.

• Applied Microscopy
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• v.27 no.4
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• pp.403-416
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• 1997

In order to observe the localization of the specific antigenic protein in the tissue of Paragonimus westermani metacercaria, immunogoldlabeling method was applied using IgG of the dog which were infected with Paragonimus westermani metacercaria and IgG of rabbits which were immunized with purified 23 kDa protein from metacercaria of the Paragenimus westermani. The metacercaria worm tissues obtained from Cambaroides similis were embedded in Lowicryl HM20 medium, treated with infected and immunized IgG and protein A gold complex (particle size; 12 nm) and observed by electron microscope. In the tissue antigen of Paragonimus westermani metacercaria, the content of excretory bladder which was highly dense electron density was constituted in the excretory bladder of the parenchymal tissue. In the metacercaria tissues antigen reacted with IgG of infected dog. Labeled gold particles distributed on the interstitial matrix of parenchymal cells, fibrous granules of parenchymal tissue and the content of excretory bladder. High antigenicity was observed on content of excretory bladder. It was found to be specifically distributed at the tissue of Paragonimus westermani metacercaria. In the tissues antigen reacted with IgG of immunized rabbit. Labeled gold particles randomly distributed on the interstitial matrix and fibrous granules of parenchymal tissue but in the content of excretory bladder of Paragonimus westermani metacercaria, gold particles were richly labeled. Therefore, the 23 kDa protein contained with Paragonimus westermani metacercaria was found protein which was specifically constituted at the content of excretory bladder of Paragonimum westermani metacercaria. The 23 kDa protein was commonly contained from of Paragonimus westermani metacercaria to adult and showed strong antigenicity against the immunized and infected IgG.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.2
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• pp.95-100
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• 1998

Seedling establishment of soybean [Glycine mar (L.) Mer-rill] is an important factor for soybean production in the field. The objective of this study was to determine the distribution of chemical composition in the emerging organs during seedling development in soybeans. Three soybean cultivars (Hill, Paldalkong, and Jangyeobkong) were planted at the Research Farm of College of Natural Resources, Korea University, on May 26, June 5, and June 14. Protein, oil, sugar, and starch contents were measured in each organ at each developing stage. Mean dry weight of three soybean cultivars decreased until VE stage and increased after this stage. Protein content of whole seedling did not change significantly during the seedling growth stage, but the amount in cotyledons markedly decreased with each growth stage increment. About 88% of the cotyledon protein was translocated to the other parts of the seedling at the V2 stage. Oil content of cotyledons sharply decreased until the V1 stage. Sugar content of the seedling was not detected at VE stage and starch content of seedlings increased slightly at VE and VC stages. For the changes of each metabolic component, the amount for whole plants decreased until the V1 stage and started to increase after this stage. The results of this study provide evidence for the breakdown of carbohydrates and oil at the initial stage of seedling growth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.15
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• pp.129-133
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• 1974

In order to obtain the fundamental informations on the protein and amylose content in rice grain, 4 varieties were subjected to the 4 different shortday treatments in 2 kinds temperature. Results can be summarized as follows; 1). By warmer temperature amylose content in the rice dropped by 2 to 3%, but no significant changes in protein content. 2). Short-day treatment caused decreases in amylose content by 2 to 5% and increases in protein content by 2 to 6%. 3). Interactions among varieties, temperature and shortday was significant for both the amylose content and protein content.

• Korean Journal of Food Science and Technology
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• v.23 no.6
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• pp.656-660
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• 1991

Heats of moisture adsorption of the sunflower nutmeat products (ground nutmeat, meal, protein concentrate and protein isolate) were determined from their isosteres at temperatures 10, 20 and $30^{\circ}C$. The changes in the heat of adsorption with moisture level were analyzed by Hunter equation. The Hunter equation was valid for representing the relationship between the heat of adsorption and the moisture content for the sunflower nutmeat products, and the accuracy-of-fit increased as protein content of the materials increased. The heat of adsorption decreased as moisture content increased, but increased as protein content increased. The heats of adsorption were 11.8-10.6 kcal/mole for the ground nutmeat at 4-12% moisture (d.b.) and 12.4-11.0 kcal/mole for the protein isolate at 6-20% moisture (d.b.).

• Food Science and Biotechnology
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• v.18 no.1
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• pp.60-65
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• 2009

Near infrared reflectance spectroscopy (NIRS) was used as a rapid and non-destructive method to determine the protein content in intact and ground seeds of pea (Pisum sativum L.) germplasms grown in Korea. A total of 115 samples were scanned in the reflectance mode of a scanning monochromator at intact seed and flour condition, and the reference values for the protein content was measured by auto-Kjeldahl system. In the developed ground and intact NIRS equations for analysis of protein, the most accurate equation were obtained at 2, 8, 6, 1 math treatment conditions with standard normal variate and detrend scatter correction method and entire spectrum (400-2,500 nm) by using modified partial least squares regression (n=78). External validation (n=34) of these NIRS equations showed significant correlation between reference values and NIRS estimated values based on the standard error of prediction (SEP), $R^2$, and the ratio of standard deviation of reference data to SEP. Therefore, these ground and intact NIRS equations can be applicable and reliable for determination of protein content in pea seeds, and non-destructive NIRS method could be used as a mass analysis technique for selection of high protein pea in breeding program and for quality control in food industry.