• 한국식품영양학회지
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• 제17권3호
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• pp.322-327
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• 2004

단백질 함량이 다른 밀가루로 데니쉬 페이스트리를 직날법으로 제조 후 급속 냉동시켜 냉동고에 12주간 저장하면서 4주 간격으로 해동ㆍ발효ㆍ굽기 한 제품의 부피, 수분함량, 조직감 및 품질평가의 결과는 다음과 같다. 1. 단백질 함량이 높은 밀가루로 만든 냉동 생지 제품의 부피가 가장 컸다. 2. 단백질 함량이 높은 밀가루로 만든 냉동 생지 제품의 수분함량이 높게 나타났으나 그 차이는 크지 않았다. 3. 단백질 함량이 높은 밀가루로 만든 냉동 생지 제품의 경도가 높게 나타났다. 4. 종합적인 품질평가는 단백질 함량이 높은 밀가루로 만든 냉동 생지 제품에서 높은 점수를 얻었다. 이상의 결과로 데니쉬 페이스트리 냉동 생지 제조시 단백질 함량이 높은 밀가루를 사용할 때 제품의 부피, 수분 함량, 품질 평가 등에서 우수한 효과를 나타냈으나 경도에는 그렇지 않았다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.100-100
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• 2020

Wheat is mainly composed of carbohydrate but it contains a moderate amount of protein, which gives a very useful characteristics to flour food such as the unique elasticity and stickiness of the dough. We developed a calibration equation for analyzing crude protein content using Near Infrared Spectroscopy to quick analyze the crude protein content of wheat germplasm stored in the National Agrobiodiversity Center, RDA, Korea. The 1,798 wheat germplasms were used to draw up the calibration formula. The crude protein's interval distribution of 1,798 wheat germplasms used for the calibration was 7.04-20.84%, the average content was 13.2%, and standard deviation was 2.6%. The germplasms distribution was composed of a suitable group for the preparation of the calibration formula because the content distribution was a normal, excluding the 13.0-15.5% content section. In order to verify the applicability of the NIRS prediction model, we measured the crude protein content of the 300 wheat germplasms that were not used for the calibration using both Kjeldahl analysis and NIR spectrum. The analysis value calculated using each method were statistically processed, and the test results and statistical indicators of the predictive model were compared. As a result, The R² value of the optimized NIRS prediction model was 0.997, and the Standard error of Calibration value(SEC) was 0.132, and slope value was 1.000. With prediction model selection, compared to Kjeldahl method, R² values were 0.994(Kjeldahl), 0.998(NIRS), and the SEC value were 0.191 and 0.132, respectively, comparing the statistical indices of the forecast model. And slope value were 1.013, 1.000, respectively. The analysis of crude protein content by the NIRS predictive model developed by each statistical index showing similar figures is judged to show a high degree of correlation with the Kjeldahl analysis. The proven calibration equation will be used to measure the crude protein content of wheat germplasms held by the National Agrobiodiversity Center, and by dividing the wheat germplasms by their use according to the crude protein content, it will provide useful information to relevant researchers.

• 약학회지
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• 제32권5호
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• pp.313-318
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• 1988

Radioprotective ginseng protein fraction was isolated from Korean white ginseng and its cytotoxic effect on CHO-K1 cells was studied by the method of measuring the relative cell survival and total cellular protein content (FRAME method). When ginseng protein at the dose of 300, 600, 900, $1200{\mu}g/ml$ was treated to cells for 24 hrs, the relative survival was significantly decreased at the concentration of above $600{\mu}g/ml$, indicating the presence of cytotoxic effect of the protein at certain concentration. When cellular protein content was measured after ginseng protein at the dose of 300, 600, 900, $1200\;{\mu}g/ml$ was treated, the amount of cellular protein was significantly reduced at the concentration above $600{\mu}g/ml$ in the case of 24 hr treatment and at all concentrations including $300{\mu}g/ml$ in the case of 72 hr treatment. The data suggest that the protein may inhibit cell growth, resulting in the reduction of live cells in culture. $ID_{50}$ value which is the concentration of ginseng protein that reduces the total cellular protein content to 50% of the control was calculated as 2276.86 and $1323.32\;{\mu}g/ml$ in groups treated for 24 and 72 hr, respectively. Since $ID_{50}$ value of above $1000{\mu}g/ml$ indicates very weak cytotoxicity, the ginseng protein seems to exert very weak cytotoxic effect on CHO-K1 cells.

• 한국작물학회지
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• 제19권
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• pp.14-20
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• 1975

파종시기 및 수확시기가 쌀의 Amylose 및 단백질함량에 미치는 영향을 알기 위하여 온도 및 일장감응성정도와 단백질함량이 다른 4개품종을 사용하여 4월10일부터 15일간격으로 5회파종하고, 출수후 30일부터 5일간격으로 6회 수확하여 미립내의 Amylose 및 단백질 함량이 품종에 따라 어떤 반응을 보이는가를 검토하였다. 그 결과를 요약하면 다음과 같다. 1. 파종시기가 늦어짐에 따라 Amylose함량은 증가하였고 단배질함량은 감소되었다. 2. 단백질함량의 파종기에 대한 반응은 품종에 따라 달라서 파종기가 지연될수록 조생종은 감소되었고 중만생종은 증가하였다. 3. 파종시기에 따른 단백질함량의 환경변이는 저단백품종보다 고단백품종에서 크게 나타났다. 4. 벼의 Amylose 및 단백질함량에 미치는 수확시기의 영향은 뚜렷하지 않았다. 5. 이상의 결과로 미루어 저Amylose 고단백계통육성을 위해서는 성숙에 지장이 없는한 파종시기를 늦추는 것이 효율이 높고 수확은 출수후 30일부터 가능할 것으로 추론하였다.

• Applied Biological Chemistry
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• 제29권2호
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• pp.116-121
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• 1986

공시소맥품종계통(供試小麥品種系統) 869에 대하여 단백질(蛋白質) 및 lysine함량(含量)을 분석(分析)하였으며, 고단백(高蛋白), 고(高) lysine품종(品種)의 환경(環境)에 대한 반응성(反應性)이 stability parameter에 의하여 검토한 결과 단백질(蛋白質)과 lysine함량(含量)은 $r=0.902^{**}$의 고도(高度)의 정(正)의 상관관계(相關關係)가 있었으나, lysine/protein과는 부(負)의 상관관계(相關關係)가 있었다. Bezostaya와 Lancota는 단백질(蛋白質)의 환경반응(環境反應)이 비교적 적은 반면 회귀계수(回歸係數)가 1.0 이상인 CI 13449와 Centurk는 환경반응(環境反應)이 컸다. 이와 같은 현상은 lysine의 환경반응(環境反應)에서도 동일한 경향을 보였다. 공시(供試)된 4개 품종(品種)의 단백질함량(蛋白質含量)의 유전력(遺傳力)은 $h^2=0.809$, lysine은 $h^2=0.647$로서 높은 유전력(遺傳力)을 보였다.

• Applied Microscopy
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• 제27권4호
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• pp.403-416
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• 1997

In order to observe the localization of the specific antigenic protein in the tissue of Paragonimus westermani metacercaria, immunogoldlabeling method was applied using IgG of the dog which were infected with Paragonimus westermani metacercaria and IgG of rabbits which were immunized with purified 23 kDa protein from metacercaria of the Paragenimus westermani. The metacercaria worm tissues obtained from Cambaroides similis were embedded in Lowicryl HM20 medium, treated with infected and immunized IgG and protein A gold complex (particle size; 12 nm) and observed by electron microscope. In the tissue antigen of Paragonimus westermani metacercaria, the content of excretory bladder which was highly dense electron density was constituted in the excretory bladder of the parenchymal tissue. In the metacercaria tissues antigen reacted with IgG of infected dog. Labeled gold particles distributed on the interstitial matrix of parenchymal cells, fibrous granules of parenchymal tissue and the content of excretory bladder. High antigenicity was observed on content of excretory bladder. It was found to be specifically distributed at the tissue of Paragonimus westermani metacercaria. In the tissues antigen reacted with IgG of immunized rabbit. Labeled gold particles randomly distributed on the interstitial matrix and fibrous granules of parenchymal tissue but in the content of excretory bladder of Paragonimus westermani metacercaria, gold particles were richly labeled. Therefore, the 23 kDa protein contained with Paragonimus westermani metacercaria was found protein which was specifically constituted at the content of excretory bladder of Paragonimum westermani metacercaria. The 23 kDa protein was commonly contained from of Paragonimus westermani metacercaria to adult and showed strong antigenicity against the immunized and infected IgG.

• 한국작물학회지
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• 제43권2호
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• pp.95-100
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• 1998

Seedling establishment of soybean [Glycine mar (L.) Mer-rill] is an important factor for soybean production in the field. The objective of this study was to determine the distribution of chemical composition in the emerging organs during seedling development in soybeans. Three soybean cultivars (Hill, Paldalkong, and Jangyeobkong) were planted at the Research Farm of College of Natural Resources, Korea University, on May 26, June 5, and June 14. Protein, oil, sugar, and starch contents were measured in each organ at each developing stage. Mean dry weight of three soybean cultivars decreased until VE stage and increased after this stage. Protein content of whole seedling did not change significantly during the seedling growth stage, but the amount in cotyledons markedly decreased with each growth stage increment. About 88% of the cotyledon protein was translocated to the other parts of the seedling at the V2 stage. Oil content of cotyledons sharply decreased until the V1 stage. Sugar content of the seedling was not detected at VE stage and starch content of seedlings increased slightly at VE and VC stages. For the changes of each metabolic component, the amount for whole plants decreased until the V1 stage and started to increase after this stage. The results of this study provide evidence for the breakdown of carbohydrates and oil at the initial stage of seedling growth.

• 한국작물학회지
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• 제15권
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• pp.129-133
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• 1974

고온과 단일이 쌀의 Amylose 및 단백질함량에 미치는 영향을 알기 위하여 일장감응성정도와 단백질 함량이 다른 4개품종을 고온(온실내)과 상온(실외)에서 전생육기간중 계속단일처리, 출수기까지만 단일처리, 출수기이후만 단일처리하고 무처리자연구와 대조하여 쌀의 Amylose 및 단백질함량을 조사비교하였다. 그 결과는 다음과 같이 요약된다. 1. 고온에 의하여 Amylose함량은 2-3% 낮아졌으나 단백질함량은 큰 변화가 없었다. 2. 단일처리로 Amylose함량을 2-5% 저하시켰고 단백질함량을 2-6%증가시켰다. 전생육기간단일에서 가장 크게 영향됐으며 출수전단일이 출수후단일보다 영향이 컸다. 3. 출수후단일조건은 조생종에서 영향이 컸고 출수전 및 전생육기간단일하에서는 고단백품종보다 저단백품종에서 영향이 컸으며 특히 감광성품종인 진흥은 출수전영향이 컸다. 4. 이상의 결과로 미루어 저Amylose 고단백계통육성을 위해서는 장일저온하의 선발이 효율이 높을 것으로 추론하였다.

• 한국식품과학회지
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• 제23권6호
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• pp.656-660
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• 1991

해바라기 종실제품(종실, 착유박, 농축단백, 분리단백)의 수분흡착열을 $10,\;20,\;30^{\circ}C$에서 측정한 등습곡선(isostere)으로부터 구하였고, 수분함량에 따른 흡착열의 변화를 Hunter 방정식에 의거하여 해석하였다. Hunter 방정식에 의한 예측치는 실측결과와 잘 일치하였으며 단백질함량이 증가할수록 정확도가 높았다. 흡착열은 수분함량이 높을수록 감소하였으며, 단백질함량이 높을수록 증가하였다. 해바라기종실의 흡착열은 건물기준 수분함량 4-12%에서 11.8-10.6 kcal/mole이었으며 분리 단백의 경우 수분함량 6-20%에서 12.4-11.0kcal/mole 이었다.

• Food Science and Biotechnology
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• 제18권1호
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• pp.60-65
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• 2009

Near infrared reflectance spectroscopy (NIRS) was used as a rapid and non-destructive method to determine the protein content in intact and ground seeds of pea (Pisum sativum L.) germplasms grown in Korea. A total of 115 samples were scanned in the reflectance mode of a scanning monochromator at intact seed and flour condition, and the reference values for the protein content was measured by auto-Kjeldahl system. In the developed ground and intact NIRS equations for analysis of protein, the most accurate equation were obtained at 2, 8, 6, 1 math treatment conditions with standard normal variate and detrend scatter correction method and entire spectrum (400-2,500 nm) by using modified partial least squares regression (n=78). External validation (n=34) of these NIRS equations showed significant correlation between reference values and NIRS estimated values based on the standard error of prediction (SEP), $R^2$, and the ratio of standard deviation of reference data to SEP. Therefore, these ground and intact NIRS equations can be applicable and reliable for determination of protein content in pea seeds, and non-destructive NIRS method could be used as a mass analysis technique for selection of high protein pea in breeding program and for quality control in food industry.