• 대한가정학회지
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• 제29권1호
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• pp.51-58
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• 1991

This study was conducted to investigate the dietary and nutritional status supplied by group feeding for blue color and white color in Seoul and Kyunggi area. The results are summarized as follows : 1. Blue color and white color of the average food supply per person per day was 1370.0g. Blue color of the total fool supply was consisted of 35.1% grain, 44.1% vegetables. White color of the total fool supply was consisted of 31.8% grain, 44.0% vegetable. The amount of milk product, fruit and fat supply were very small. 2. Blue color and white color of the ratio of main dish and side dish supplied was 69 : 31, 60.0 : 39.7. 3. Main dish all is rice. Side dish ard kinds of soupes and Jigeai Johngol in the two groups. In the kind of side dish white color much more than blue color. Dessert are scarcely supplied in the two groups. 4. The mean daily supplies of energy and 9 nutrients were above the level of the R.D.A. Blue color and white color of the mean daily amount of energy supply was 2653.0㎉ 3022.6㎉. Blue color and white color of the ratio of carbohydrate, protein, and fat supplied was 68.4%, 18.6%, 13.1%, and 62.1%, 20.9%, 17.0%. 5. Blue color and white color of the average feeding cost amounts to 460won, 812won per meal. 6. Blue color; There were high correlations in supply amoung energy, protein, fat. Carbohydrate, calcium, iron, riboflavin, niacin. White color; There were high correlation in supply amoung energy, protein, carbohydrate, calcium, iron, thiamin, riboflavin, niacin, vit-C.

• 대한지역사회영양학회지
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• 제3권2호
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• pp.210-217
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• 1998

The purpose of this study was to examine changes in energy and macronutrient intakes during the premenstrual(days -10-1) and postmenstrual(days 5-14) phases. Thirty-two college female students recorded their food consumption and dietary patterns over 5 weeks from September 8 to October 12, 1997. Mean daily energy and macronutrient intakes and the percentage of energy intake from protein and carbohydrate were relatively consistent over the two menstrual cycle phases, but the energy intake from fat increased 1%(P<0.05)during the postmenstrual phase. Two different dietary patterns were identified by the shifts in energy intake between postmenstrual and premenstrual phases. Seventy five percent of subjects revealed a consistent pattern with the results of previous studies investigating food intake over the menstrual cycle, in which the premenstrual phase is associated with an increment in energy, carbohydrate, and protein intake. In contrast, energy, protein, and fat intakes increased during the postmenstrual phase in 25% of the subjects. We conclude that cyclical fluctuations in energy and macronutrient intakes occur in both postmenstrual and premenstrual dietary pattern groups across the two menstrual phased with the opposite directions.

• Journal of Nutrition and Health
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• 제30권2호
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• pp.147-154
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• 1997

This study was conducted to evaluate dietary fiber intake, iron status, and their correlations in 50 female college students living in Seoul. The nutritional status was analyzed using 3-day dietary record, duplicated diet collection, and venous blood sampling. The mean values for age, height, weight, BMI, and blood pressure of the subjects were 23.2 years, 160.2cm, 53.9kg, 21.0kg/$m^2$, and 110.1/68.4mmHg, respectively. Daily intakes of enery, protein, fiber, crude fiber, iron, and heme iron were 1635.5㎉, 54.3g, 22.5g, 6.8g, 16.2mg, and 0.2mg, respectively. Fiver intake was positively correlate with energy, protein, carbohydrate, vitamin C, iron, and crude fiber intakes. Also, iron intake was positively correlated with energy, protein, lipid, carbohydrate, and vitamin C intakes. There was a significant correlation between heme iron and MFP(meat, fish, poultry) intakes. To exame the iron balance, iron intake and excretion were measured. Iron intake and excretions through urine and feces were 19.5mg, 8.5mg, and 1.6mg, respectively. Based on these iron retention and iron apparent absorbability were calculated as 9.4mg and 52.4%, respectively. There was no significant relationship between dietary fiber intake and iron status. However, there were significant positive correlation between fiber intake and urinary iron excretion.

• Journal of Nutrition and Health
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• 제25권1호
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• pp.12-21
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• 1992

This study was aimed to investigate the relations between self-selected intake of 3 macronutrie-nts and body weight gain and body fat accumulation in male rats given three isocaloric diets differing carbohydrate protein and fat contents concurrently. Also the effect of dietary restriction was observed. Forty two male rats of Sprague-Dawley strain weighing 68.7$\pm$6.1g were randomly divided into 2 groups and were allowed to have foods from 3 different cups for 8-hours a day or ad libitum, After 12 weeksthey were decapitated and their brains were quickly removed and frozen until they were assayed for serotonin and its metabolite 5-HIAA The carcass was dried at 105$\pm$2$^{\circ}C$ and measured the contents of body water and body fat. The animals chose a moderately high and constant carbohydrate level and showed the increase of percent protein intake with age and great individual variations. Protein in the diet seemed to trigger appetite and increase food intake which resulted in higher weight gains and in more fat deposition in the body. The concentration of brain serotonin did not show any correlations with the intake of nutrients. the accumulation of body fat and the gain of body weight.

• Asian-Australasian Journal of Animal Sciences
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• 제13권3호
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• pp.376-380
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• 2000

This study was conducted to investigate protein and carbohydrate utilization of Micrococcus varians, Staphylococcus carnosus and Staphylococcus xylosus. Sensitivity to pH, sodium chloride, potassium sorbate and sodium nitrite of these strains was also determined. In Chinese-style beaker sausage manufacturing, the growth rate of these strains during the curing period ($20^{\circ}C$ and $30^{\circ}C$) was evaluated. The results indicated that no strains could hydrolyze azo-casein and sarcoplasmic protein and only S. xylosus could hydrolyze gelatin at $30^{\circ}C$. All of these strains could oxidize and ferment fructose and mannitol. S. carnosus and S. xylosus could slightly oxidize lactose and utilize citrate. Arabinose was oxidized by S. xylosus and sorbitol was oxidized by S. carnosus. Growth of M. varians was restricted at pH 5.0 and S. carnosus and S. xylosus were restricted at pH 4.5. S. xylosus and S. carnosus were able to grow with 0.1~0.5% potassium sorbate, 50~200 ppm sodium nitrite or 1~15% sodium chloride. S. xylosus had a higher growth rate than the other strains. Staphylococcus species grew well during curing period of Chinese-style beaker sausage then followed by Micrococcaceae.

• Asian Pacific Journal of Cancer Prevention
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• 제16권8호
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• pp.3425-3428
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• 2015

Objective: To explore the association of serum tumor abnormal protein (TAP) with other serological biomarkers e.g. carcinoembryonic antigen (CEA), carbohydrate antigen 125 (CA125), carbohydrate antigen 19-9 (CA19-9) and its clinical application in colorectal cancer (CRC) patients. Methods: Patients (N=98) were enrolled into this study with histologically or cytologically confirmed CRC. Using a test kit, the level of TAP was determined, while chemiluminescence was used to measure the levels of some other common serological biomarkers e.g. CEA, CA125 and CA19-9. Results: The area of TAP condensed particulate matter decreased after chemotherapy compared with before chemotherapy when CT or MRI scans showed disease control. In contrast, it increased with disease progression (P<0.05). Furthermore, a statistically significant difference was confirmed in monitoring of TAP and common serological biomarkers e.g. CEA and CA19-9 (p<0.05). Conclusions: Detecting TAP in CRC patients has high sensitivity and specificity and can be used as a new independent indicator for clinically monitoring CRC patients in the course of chemotherapy.

• 대한수의학회지
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• 제37권3호
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• pp.547-554
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• 1997

Galectin-3 is known as an animal ${\beta}$-galactoside-binding lectin charicterized with S-type carbohydrate recognition domain. It plays a role in growth, adherence and movement of cells. It is, also, related to the cell transformation and metastasis of tumor cells. In this study, we have expressed and purified recombinant human galectin-3 (rHgalectin-3) using E coli system and asialofetuin affinity chromatography for the future development of monoclonal antibody to Hgalectin-3, which is suggested as the tumor marker for the gastric and thyroid gland cancers. Expressed protein was confirmed as the Hgalectin-3 by immunoblot with cross-reactive murine monoclonal antibody. Lectin activity and specificity of purified protein were, also, confirmed by the competitive inhibition with galectin-3 specific carbohydrate, lactose. Like physiological galectin-3, lectin activity of the molecule was not changed in nonreduced condition. Dimer formation, furthermore, was observed at high concentration of the protein even in the reduced condition, which is well known in physiological galectin-3. These results showed purified rHgalectin-3 has the same activity and molecular nature compared to the physiological galectin-3.

• 한국동물학회지
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• 제33권2호
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• pp.166-174
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• 1990

꿀벌부채명나방이 무늬수종다리좀벌에 의해 기생 되었을때 이 기주의 혈림프에서 대사물질의 변화가 생겼다. 이 기새벌에 의해 기생된 기주와 기생되지않은 기주의 혈림프에서 총단백질과 총탄수화물의 농도를 비교하여보면, 기생된 기주에서 전체적으로 뚜렷이 감소하는 경향을 나타내었다.그러나, 총유리아미노산은 기생된 후 첫날을 제외하고 기생된 기주에서 전체적으로 뚜렷이 증가하는 경향을 나타내었다. 그리고 유리아미노산 중에서 glycine, proline, arginine, isoleucine, phenylalanie, leucine,valine, glutamk acid,histidine, serine, tyrosine은 뚜렷이 증가하는 경향을 나타내었고,threonine, aspartic acid, methionine, cysteine, alanine,lysine는 약간의 감소하는 경향을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.733-738
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• 2007

An extracellular exoinulinase($2,1-\beta-D$ fructan fructanohydrolase, EC 3.2.1.7), which catalyzes the hydrolysis of inulin into fructose and glucose, was purified 23.5-fold by ethanol precipitation, followed by Sephadex G-100 gel permeation from a cell-free extract of Kluyveromyces marxianus YS-1. The partially purified enzyme exhibited considerable activity between pH 5 to 6, with an optimum pH of 5.5, while it remained stable(100%) for 3 h at the optimum temperature of $50^{\circ}C$. $Mn^{2+}\;and\;Ca^{2+}$ produced a 2A-fold and 1.2-fold enhancement in enzyme activity, whereas $Hg^{2+}\;and\;Ag^{2+}$ completely inhibited the inulinase. A preparation of the partially purified enzyme effectively hydrolyzed inulin, sucrose, and raffinose, yet no activity was found with starch, lactose, and maltose. The enzyme preparation was then successfully used to hydrolyze pure inulin and raw inulin from Asparagus racemosus for the preparation of a high-fructose syrup. In a batch system, the exoinulinase hydrolyzed 84.8% of the pure inulin and 86.7% of the raw Asparagus racemosus inulin, where fructose represented 43.6mg/ml and 41.3mg/ml, respectively.

• 한국식품과학회지
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• 제22권4호
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• pp.439-444
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• 1990

본 연구에서는 우리나라에서 재배되는 14품종의 콩으로 제조한 두부의 화학적조성, 수율과 관능적인 특성을 비교하였다. 두부의 제조는 콩을 침지하여 마쇄한 후 가열 및 여과하여 $CaSO_4$로 응고 시킨 다음 압착, 성형하였다. 그 결과 제조된 두부의 수분은 $75.0{\sim}82.0%$이었으며, 건물량으로서 단백질함량은 $48.6{\sim}56.1%$, 지방은 $14.8{\sim}40.4%$, 탄수화물은 $6.4{\sim}26.8%$였다. 두부의 부피수율 및 고형분수율은 콩 100g당 각각 $182.2{\sim}227.5cm^3,\;42.65{\sim}55.60%$, 단백질수율은 $57.90{\sim}76.50%$였다. 14품종 중 수원-141로 제조한 두부가 부피수율, 수분함량 및 탄수화물함량이 제일 높았으며 지방함량은 가장 낮았다. 백운콩과 장엽콩으로 만든 두부는 수분, 단백질 및 지방함량이 매우 낮았고 부피수율도 가장 낮았음을 볼 때 두부의 수분, 단백질, 탄수화물이 부피수율과 밀접한 관계가 있음을 알 수 있다. 두부의 냄새와 맛은 품종간에 많은 차이가 있었으나 관능적 특성과 화학적 조성간의 상관관계는 높지 않았다.