• Title/Summary/Keyword: Propagation methods

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Isolation and molecular characterizations of canine distemper virus from a naturally infected Korean dog using Vero cells expressing dog signaling lymphocyte activation molecule

  • Yang, Dong-Kun;Kim, Ha-Hyun;Lee, Siu;Yoon, Yoon-Seek;Park, Jungwon;Oh, Dongryul;Yoo, Jae Young;Ji, Miryeon;Han, Bokhee;Oh, Subin;Hyun, Bang-Hun
    • Journal of Veterinary Science
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    • v.21 no.5
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    • pp.64.1-64.14
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    • 2020
  • Background: Canine distemper virus (CDV) infection results in high morbidity and mortality in dogs. There has been no report about Isolation of Korean CDV since 1980 in Korea. Objectives: To investigate the biological properties and the genetic characterization of Korean CDV. Methods: Vero cells expressing dog signaling lymphocyte activation molecule (dSLAM) gene named as Vero/dSLAM were used to isolate CDV using 17 samples. Diagnostic methods such as cytopathic effects, immunofluorescence assay, peroxidase linked assay, electron microscopy, rapid immunodiagnostic assay, and reverse transcription polymerase chain reaction were used to confirm the Korean CDV isolate as a CDV. The genetic analysis was performed through cloning and sequencing of hemagglutinin gene of CDV isolate. Results: A virus propagated in Vero/dSLAM cell was confirmed as CDV (CD1901 strain) based on the above methods. The CD1901 strain showed the highest viral titer (105.5 50% tissue culture infectious dose [TCID50]/mL) in the Vero/dSLAM cells at 4 days post inoculation, but did not form a fork on chorioallantoic membrane of 7-day-old egg. Ribavirin, a nucleotide analogue anti-viral agent, inhibits moderately the Korean CDV propagation in the Vero/dSLAM cells. The nucleotide and amino acid sequences of the H gene of CD1901 strain were compared with those of other CDV strains. The CD1901 strain belonged to Asia 1 group and had the highest similarity (99.9%) with the BA134 strain, which was isolated in China in 2008. Conclusions: We constructed successfully Vero/dSLAM and isolated one Korean CDV isolate (CD1901 strain) from a naturally infected dog. The CD1901 strain belonged to Asia 1 genotype.

Design of Traffic Sequence for the Maritime Data Communications in HF band (HF대 해상 데이터통신을 위한 통신시퀜스 설계)

  • Go, Yun-Gyu;Lee, Yeung-Su;Choi, Jo-Cheon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2008.05a
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    • pp.81-85
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    • 2008
  • The INMARSAT is able to long range maritime communications that can not use for expensive charge in small ship. Additional an opinion of unuseful NBDP that is international discussion for replacement methods for the effective data communications by using HF band. A feature of HF band communication is ionospheric propagation that have not the distance question as A2, A3 and A4 sea areas. Therefore all navigation ship should has supplied service such as MSI, VMS, E-mail beside of distress and public communication that is demanded a design of communication sequence for using SSB transceiver. This paper has designed the new packet and communication sequence of truly and automatically radio link for maritime data communications by SSB in HF band.

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Bond Properties of CFRP Rebar in Fiber Reinforced High Strength Concrete with Surface Treatment Methods of Reinforcing Fibers (보강섬유의 표면처리에 따른 섬유보강 고강도콘크리트와 CFRP 보강근의 부착특성)

  • Park, Chan-Gi;Won, Jong-Pil;Cha, Sang-Sun
    • Journal of the Korea Concrete Institute
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    • v.21 no.3
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    • pp.275-282
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    • 2009
  • The effects of surface treatment method of reinforcing fiber on the bonding strength between carbon fiber reinforced polymer rebar (CFRP rebar) and high strength concrete have been evaluated in this study. The structural PVA fiber is coated with a proprietary hydrophobicoiling agent and crimped type polyolefin based structural synthetic fiber is deformed with a geometrical modification were used for the reinforcing fiber. The compressive tests have been performed to evaluate the strength property of high strength concrete depending on the surface treatment method of fiber. The bonding property between the high strength concrete and the CFRP rebar was evaluated by means of direct bonding test. The test results indicated that the surface treatment method of fiber effect on the bonding behavior of high strength concrete and CFRP rebar. Also, as the development and propagation of splitting cracks were controled by adding fibers into the high strength concrete, the bonding behavior, bond strength and relative bonding strength of CFRP rebar and high strength concrete were significantly improved.

A Study on Ray Tracing Method for Wave Propagation Prediction with Acceleration Methods (가속 방법을 이용하는 전파 광선 추적법에 관한 연구)

  • Kwon, Se-Woong;Moon, Hyun-Wook;Oh, Jae-Rim;Lim, Jae-Woo;Bae, Seok-Hee;Kim, Young-Gyu;Park, Joung-Soo;Yoon, Young-Joong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.5
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    • pp.471-479
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    • 2009
  • In this paper, we proposed an improved ray tracing method with an amelioration of visible tree structure, a visible face determination method, and non-uniform random test point method. In a proposed visible tree structure, it reduces tree nodes by means of merging similar nodes. In a visible face determination method, it shows that a ray hit test with a packet ray method can reduce a test time. A ray tracing method involving with a packet ray hit test method can improve a tree construction time up to 3.3 times than a ray tracing method with a single ray hit test method. Furthermore, by seeding a non-uniform and random test point on a face, tree construction time is improved up to 1.11 times. Received powers from the proposed ray tracing results and measured results have good agreement with 1.9 dB RMS error.

Estrus Detection in Sows Based on Texture Analysis of Pudendal Images and Neural Network Analysis

  • Seo, Kwang-Wook;Min, Byung-Ro;Kim, Dong-Woo;Fwa, Yoon-Il;Lee, Min-Young;Lee, Bong-Ki;Lee, Dae-Weon
    • Journal of Biosystems Engineering
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    • v.37 no.4
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    • pp.271-278
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    • 2012
  • Worldwide trends in animal welfare have resulted in an increased interest in individual management of sows housed in groups within hog barns. Estrus detection has been shown to be one of the greatest determinants of sow productivity. Purpose: We conducted this study to develop a method that can automatically detect the estrus state of a sow by selecting optimal texture parameters from images of a sow's pudendum and by optimizing the number of neurons in the hidden layer of an artificial neural network. Methods: Texture parameters were analyzed according to changes in a sow's pudendum in estrus such as mucus secretion and expansion. Of the texture parameters, eight gray level co-occurrence matrix (GLCM) parameters were used for image analysis. The image states were classified into ten grades for each GLCM parameter, and an artificial neural network was formed using the values for each grade as inputs to discriminate the estrus state of sows. The number of hidden layer neurons in the artificial neural network is an important parameter in neural network design. Therefore, we determined the optimal number of hidden layer units using a trial and error method while increasing the number of neurons. Results: Fifteen hidden layers were determined to be optimal for use in the artificial neural network designed in this study. Thirty images of 10 sows were used for learning, and then 30 different images of 10 sows were used for verification. Conclusions: For learning, the back propagation neural network (BPN) algorithm was used to successful estimate six texture parameters (homogeneity, angular second moment, energy, maximum probability, entropy, and GLCM correlation). Based on the verification results, homogeneity was determined to be the most important texture parameter, and resulted in an estrus detection rate of 70%.

고효율 LED 제작을 위한 비,반극성 GaN의 성장 및 결함 분석

  • Gong, Bo-Hyeon;Kim, Dong-Chan;Kim, Yeong-Lee;An, Cheol-Hyeon;Bae, Yeong-Suk;U, Chang-Ho;Seo, Dong-Gyu;Nam, Ok-Hyeon;Yu, Geun-Ho;Jang, Jong-Jin
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.172-172
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    • 2009
  • In this study, we presented comparative discrimination methods to identify various line and planar defects observed in nonpolar a-GaN epilayers on r-sapphire substrates. Unlike the case of conventional c-GaN, which is dominated by perfect threading dislocations, systematic identification of undistinguishable defects using transmission electron microscopy (TEM) is necessary to suppress the propagation of defects in nonpolar GaN epilayers. Cross-sectional TEM images near the [0001] zone axis revealed that perfect mixed and pure screw type dislocations are visible, while pure edge, partial dislocations, and basal stacking faults (BSFs) are not discernible. In tilted cross-sectional TEM images along the [$1\bar{2}10$] zone axis, the dominant defects were BSFs and partial dislocations for the $g=10\bar{1}0$ and 0002 two-beam images, respectively. From plan view TEM images taken along the [$11\bar{2}0$] axis, it was found that the dominantpartial and perfect dislocations were Frank-Shockley with b=${\pm}1/6$<$20\bar{2}3$> and mixed type without an 1 component including b=${\pm}1/3$<$1\bar{2}10$> and ${\pm}1/3$<$\bar{2}110$>, respectively. Prismatic stacking faults were observed as inclined line contrast near the [0001] zone axis and were visible as band contrast in the two-beam images along the [$1\bar{2}10$] and [$11\bar{2}0$] zone axes.

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Delay Spread Measurement and Analysis in 3 GHz and 6 GHz Indoor Environments (3 GHz, 6 GHz 실내 환경의 지연 확산 측정 및 분석)

  • Lee, Seong-Hun;Lee, Hwa-Choon;Cho, Byung-Lok
    • The Journal of the Korea institute of electronic communication sciences
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    • v.15 no.1
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    • pp.15-20
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    • 2020
  • In this paper, delay diffusion for exhibition hall and conference room was measured and analyzed in the indoor environment of the building. Scenarios for the indoor environment of the two buildings were written. also, the system configuration and measurement methods were conducted under the same conditions. The measurement conditions were set to 3 GHz and 6 GHz of center frequencies and analysis band selected 2 GHz. The measurement system consisted of vector network analyzer, power amplifier, omni-directional transmit and receive antenna, and transmission line. According to the indoor environment scenario of the two buildings, the location of the receiving antenna was divided into three zones based on the location of the transmitting antenna and this was measured at 1 m intervals according to 18 locations. The power delay profiles, RMS delay spread, and K-factor results of two buildings were compared and analyzed.

RF Collimator Design having Multi-Dielectric Structure using the Phase Field Design Method (페이즈필드 설계법을 이용한 다중 유전체 구조의 RF 콜리메이터 설계)

  • Go, Joohyun;Seong, Hong Kyoung;Kim, Hanmin;Park, Jinwoo;Yoo, Jeonghoon
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.31 no.1
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    • pp.47-52
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    • 2018
  • In this study, a collimator composed of multi-dielectric structures is designed using the phase field design method, a kind of topology optimization methods. It is also purposed to improve the mechanical-structural performance of a collimator by replacing previously used air regions with another dielectric material. Polypropylene and paraffin are selected as the dielectric materials for the design process taking manufacturability into account. The design objective is formulated by integrating the intensity of the electromagnetic field in the pre-determined target area to realize the collimating performance. The model for accurate numerical analysis was derived from the final result obtained from the design process through the simple cut-off method and it shows the improved performance of 105% compared with the free space wave propagation. For the designed model, the possibility of reverse transformation, the mechanical durability evaluation under the compression load, and the electromagnetic performance in the X-band range were also evaluated.

Acoustic parabolic equation model with a directional source (방향성 있는 음원이 적용된 음향 포물선 방정식 모델)

  • Lee, Keunhwa;Na, Youngnam;Son, Su-Uk
    • The Journal of the Acoustical Society of Korea
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    • v.39 no.1
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    • pp.1-7
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    • 2020
  • The acoustic parabolic equation method in the ocean is an efficient technique to calculate the acoustic field in the range-dependent environment, emanating from a point source. However, we often need to use the directional source with a main beam in the practical problem. In this paper, we present two methods to implement the directional source in the acoustic parabolic equation code easily. One is simply to filter the Delta function idealized as an omni-directional point source. Another method is based on the rational filtering of the self-starter solution. It has a limitation not to separate the up-going and the down-going wave for the depth, but would be useful in implementing the mode propagation. Numerical examples for validation are given in the Pekeris environment and the deep sea environment.

Hole-Filling Method for Depth-Image-Based Rendering for which Modified-Patch Matching is Used (개선된 패치 매칭을 이용한 깊이 영상 기반 렌더링의 홀 채움 방법)

  • Cho, Jea-Hyung;Song, Wonseok;Choi, Hyuk
    • Journal of KIISE
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    • v.44 no.2
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    • pp.186-194
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    • 2017
  • Depth-image-based rendering is a technique that can be applied in a variety of 3D-display systems. It generates the images that have been captured from virtual viewpoints by using a depth map. However, disoccluded hole-filling problems remain a challenging issue, as a newly exposed area appears in the virtual view. Image inpainting is a popular approach for the filling of the hole region. This paper presents a robust hole-filling method that reduces the error and generates a high quality-virtual view. First, the adaptive-patch size is decided using the color and depth information. Also, a partial filling method for which the patch similarity is used is proposed. These efforts reduce the error occurrence and the propagation. The experiment results show that the proposed method synthesizes the virtual view with a higher visual comfort compared with the existing methods.