• The Korean Journal of Pain
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• 제34권1호
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• pp.19-26
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• 2021

Background: Prolotherapy is a proliferation therapy as an alternative medicine. A combination of dextrose solution and lidocaine is usually used in prolotherapy. The concentrations of dextrose and lidocaine used in the clinical field are very high (dextrose 10%-25%, lidocaine 0.075%-1%). Several studies show about 1% dextrose and more than 0.2% lidocaine induced cell death in various cell types. We investigated the effects of low concentrations of dextrose and lidocaine in fibroblasts and suggest the optimal range of concentrations of dextrose and lidocaine in prolotherapy. Methods: Various concentrations of dextrose and lidocaine were treated in NIH-3T3. Viability was examined with trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Migration assay was performed for measuring the motile activity. Extracellular signal-regulated kinase (Erk) activation and protein expression of collagen I and α-smooth muscle actin (α-SMA) were determined with western blot analysis. Results: The cell viability was decreased in concentrations of more than 5% dextrose and 0.1% lidocaine. However, in the concentrations 1% dextrose (D1) and 0.01% lidocaine (L0.01), fibroblasts proliferated mildly. The ability of migration in fibroblast was increased in the D1, L0.01, and D1 + L0.01 groups sequentially. D1 and L0.01 increased Erk activation and the expression of collagen I and α-SMA and D1 + L0.01 further increased. The inhibition of Erk activation suppressed fibroblast proliferation and the synthesis of collagen I. Conclusions: D1, L0.01, and the combination of D1 and L0.01 induced fibroblast proliferation and increased collagen I synthesis via Erk activation.

• The Korean Journal of Pain
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• 제19권1호
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• pp.77-80
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• 2006

Background: Painful experiences during procedures such as prolotherapy and intramuscular stimulation are stressful to patients and can affect the treatment outcome. We present a method for relieving pain and increasing the level of patient comfort during the procedure. Methods: Twenty six patients who requested sedation anesthesia during the procedure were examined. All patients were injected with 500 ml of 0.9% normal saline and were monitored by electrocardiography, blood pressure and pulse oximetry. The patients were supplied with oxygen (3 L/min) through a nasal cannula. Midazolam (0.02 mg/kg) and alfentanil ($8{\mu}g/kg$) was injected before the procedure and a bolus injection was administered during the procedure if patients felt any pain. The duration of the procedure, the total amount of drugs, the changes in the systolic blood pressure, heart rate, pulse oxygen saturation, sedation and pain level during procedure, satisfaction scale after the procedure, complications and the incidence of amnesia were evaluated. Results: Twenty one patients had a moderate level of sedation, 15 patients did not feel any pain during the procedure, 17 patients had high level of satisfaction (8-10). No patient experienced complications after the procedure, or unstable vital signs, and 6 patients could not remember the procedure. Conclusions: Sedation anesthesia is a safe method for relieving pain during the procedure, and most patients had a high level of satisfaction.

• 대한정형외과스포츠의학회지
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• 제9권2호
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• pp.109-113
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• 2010

목적: 견관절 유착성 관절낭염 환자에서 초음파 관찰 하에 오구 상완 인대 부위에 시행한 증식제제와 스테로이드제 병합 주사요법의 효과에 대해 알아보고자 한다. 대상 및 방법: 견관절 유착성 관절낭염으로 진단받은 환자들 중 초음파 관찰 하에 오구 상완 인대부위에 시행한 증식제제와 스테로이드제 병합주사요법을 시행 받은 환자들로 최소 1년 이상 추시 가능하였던 53예를 대상으로 견관절의 상태를 운동범위와 VAS 점수를 증식치료 전과 치료 8주 후 그리고 치료 1년 후의 최종 추시 상태를 측정 비교하여 평가하였다. 결과: 견관절 유착성 관절낭염 환자의 평균 추시 기간은 13.8개월이었으며, 증세 발현부터 병원 내원시까지 걸린 기간은 평균 22.5개월이었다. 주입 횟수는 평균 1.82회, 관절운동 범위는 치료 전 굴곡 93.4도, 외전 79.2도 였으며 VAS 점수는 평균 6.7로 측정되었다. 치료 8주 후 굴곡 142도, 외전 125.4도, VAS 점수는 3.5로 호전되었고, 치료 1년 후 최종 추시 상 굴곡은 153도, 외전 152.6도로 유지되었으며, VAS 점수는 3.7로 유지되었다. 결론: 견관절 유착성 관절낭염 환자에서 초음파 관찰 하에 오구 상완 인대 부위에 시행하는 증식제제와 스테로이드제 병합치료는 통증 호전과 관절운동의 회복시킬 수 있는 방법 중 하나로 사료된다.

• 척추신경추나의학회지
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• 제11권2호
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• pp.57-64
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• 2016

This is a case report about left leg radiating pain treated with complex traditional Korean medical intervention, while the case did not responded to prolotherapy and extracorporeal shock wave treatment for lumbar herniated intervertebral disc (HIVD). The patient was diagnosed with a piriformis problem after a physical examination and was treated with lumbar and piriformis muscle treatment. After 9 sessions of treatment for 33 days, Numeric Rating Scale (NRS) for pain has been decreased from 8 to 3. Further studies with more cases and longer observation period should be conducted to provide evidence for optimum acupuncture procedure considering its effectiveness, safety and patients' compliance altogether.

• 대한견주관절학회:학술대회논문집
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• 대한견주관절학회 2008년도 제16차 학술대회
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• pp.21-22
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• 2008

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제49권2호
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• pp.59-60
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• 2023

• 한국임상수의학회지
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• 제40권2호
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• pp.93-103
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• 2023

The tendon is a dense connective tissue that connects muscle to bone and plays an essential role in joint motion. The injured tendon heals slowly owing to its low cellularity and vascularity. This study aimed to evaluate and compare the effects of regenerative injection therapy (RIT), 20 % dextrose prolotherapy (DP), and platelet-rich plasma (PRP) injections that can promote tendon healing. Twenty-one New Zealand white rabbits were divided into the control, DP, and PRP treatment groups. The superficial digital flexor tendon (SDFT) of the right hindlimb of each rabbit was used. A round defect of 2 mm was induced. Approximately 0.2 mL of 20% dextrose and autologous PRP were injected into the proximal and distal ends of the SDFT mass. Radiographic and ultrasonographic examination and cross-sectional area (CSA) calculations were performed pre-operatively and at 2, 4, and 8 weeks. The SDFT of both limbs was transected for biomechanical and histomorphometric evaluations. The SDFT of the left limb was transected for intact control. Semi-quantitative analysis was performed to evaluate the histomorphometric properties. Additional analysis was performed using H&E, Masson's trichrome, and immunohistochemical staining. The biomechanical evaluation showed that the treatment groups had higher tensile strength compared to the defect control group, while the PRP group had higher tensile strength than the DP group. On histological examination, the treatment groups appeared to be relatively closer to the remodeling phase of the healing process than the defect control group; the characteristics of the PRP group were closer to the remodeling phase than those of the DP group. The ultrasonographic examination showed different tendencies. Increased values in the CSA were observed during the early period in the treatment groups. This study suggests that PRP and DP can promote the healing of tendon injury, and these effects were superior with PRP than that with DP.

• 한국임상수의학회지
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• 제40권3호
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• pp.242-242
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• 2023

• 대한추나의학회지
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• 제3권1호
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• pp.167-174
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• 2002

흉요추 이행부의 안정성 압박골절은 통증의 소실과 후만각 증가 억제에 의한 척추의 안정화이다. 이의 원인을 후방인대의 손상에 의한 손상으로 보고 프로로테라피(Prolotherapy)로 이의 치료를 시행 할 수 있다고 생각되며 한방적인 방법으로 화침을 적용할 수 있었다. 최근 저자는 흉요추 이행부의 안정성 압박골절에 의한 요배통 환자를 발견하고 화침치료를 통해 증세 회복에 도움이 되었기에 치료내용과 경과를 문헌고찰과 함께 보고하는 바이다.

• 대한정형외과 초음파학회지
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• 제6권1호
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• pp.15-19
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• 2013

소아에서 발생하는 슬와 낭종은 증상이 없는 경우가 대부분이며 합병증을 유발하는 경우도 찾아보기 어렵다. 저자는 관절 내 병변이 동반되지 않은 슬와 낭종을 가진 한 명의 소아에서 경골 신경과 슬와 동맥 압박이 관찰되어 조기 감압술과 증식치료를 이용하여 치료하였고, 이를 문헌 고찰과 함께 보고하고자 한다.