• 제목/요약/키워드: Progeny test

검색결과 89건 처리시간 0.021초

Agrobacterium 운반체를 이용한 유채의 형질전환: 식물체재분화와 후대검정 (Transformation of Brassica napus via Agrobacterium Vector : Plant Regeneration and Progeny Analysis)

  • KIM, Kyung Min;SOHN, Jae Keun;CHUNG, Jae Dong
    • 식물조직배양학회지
    • /
    • 제24권5호
    • /
    • pp.269-272
    • /
    • 1997
  • 유채의 자엽조직을 rolC 유전자를 가진 Agrobacterium 운반체와 공동배양하여 형질전환체를 얻고, 후대에 대한 유전분석을 실시하였다. Arobacterium 운반체와 공동배양된 유채의 자엽조직을 0.5mg/L NAA, 2.0mg/L BA, 30mg/L kanamycin, 100mg/L cefotaxime, 30mg/L sucrose, 3mg/L $\textrm{AgNO}_{3}$ 및 2 g/L Gelrite가 첨가된 MS 배지에 배양하였을 때 형질전환된 식물체의 분화율이 17.5%로 나타났다. 형질전환체로 확인된 식물체를 자가수정시켜 얻어진 후대에 대한 유전분석을 실시한 바 rolC 유전자가 후대로 유전됨을 확인 할 수 있었다.

  • PDF

Optimization of Swine Breeding Programs Using Genomic Selection with ZPLAN+

  • Lopez, B.M.;Kang, H.S.;Kim, T.H.;Viterbo, V.S.;Kim, H.S.;Na, C.S.;Seo, K.S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제29권5호
    • /
    • pp.640-645
    • /
    • 2016
  • The objective of this study was to evaluate the present conventional selection program of a swine nucleus farm and compare it with a new selection strategy employing genomic enhanced breeding value (GEBV) as the selection criteria. The ZPLAN+ software was employed to calculate and compare the genetic gain, total cost, return and profit of each selection strategy. The first strategy reflected the current conventional breeding program, which was a progeny test system (CS). The second strategy was a selection scheme based strictly on genomic information (GS1). The third scenario was the same as GS1, but the selection by GEBV was further supplemented by the performance test (GS2). The last scenario was a mixture of genomic information and progeny tests (GS3). The results showed that the accuracy of the selection index of young boars of GS1 was 26% higher than that of CS. On the other hand, both GS2 and GS3 gave 31% higher accuracy than CS for young boars. The annual monetary genetic gain of GS1, GS2 and GS3 was 10%, 12%, and 11% higher, respectively, than that of CS. As expected, the discounted costs of genomic selection strategies were higher than those of CS. The costs of GS1, GS2 and GS3 were 35%, 73%, and 89% higher than those of CS, respectively, assuming a genotyping cost of $120. As a result, the discounted profit per animal of GS1 and GS2 was 8% and 2% higher, respectively, than that of CS while GS3 was 6% lower. Comparison among genomic breeding scenarios revealed that GS1 was more profitable than GS2 and GS3. The genomic selection schemes, especially GS1 and GS2, were clearly superior to the conventional scheme in terms of monetary genetic gain and profit.

유색미(有色米) 수집종(蒐集種)에 대한 특성(特性) 연구(硏究) I. 유색미(有色米) 수집종(蒐集種)에 대한 식물학적(植物學的) 특성(特性) (Studies on the Collected Color Rice I. Botanical Characteristics for Color Rice Collected)

  • 최현구;정재영;이희봉;정종태;김보경
    • 농업과학연구
    • /
    • 제27권2호
    • /
    • pp.71-80
    • /
    • 2000
  • 유색미 27개의 국내외 수집종에 대한 식물학적 및 이삭 특성을 비교 분석한 결과는 다음과 같다. 간장은 대조구인 흑진주벼보다 대부분이 크게 나타났는데 외래종인 Denorado가 113.1cm로 가장 컸다. 분얼수, 유효분얼수에서는 대조구인 흑진주벼와 큰 차이가 없었으나 Iksan #440의 유효분얼수가 18.0으로 가장 높았다. 수장에서는 Denorado가 27.3cm로 높게 나타났고 1차지경수에서는 15.27개로 Milyang #153가 가장 높은 반면에 2차지경수에서는 Iksan #428가 39.87"로 가장 많았다. 립장은 Sinucninul이 10.1mm로 가장 길었고 립폭은 흑진주벼가 2.53mm로 가장 두꺼웠다. 천립중은 Denorado가 31.44g으로 가장 높았고 Ou #3497가 17.15g으로 가장 낮게 나타났다. 공시계통에 대해 Suwon#432 ${\times}$ Heukmi#940이 8의 $F_1$이 주당 분얼수, 수장, 1000 립중에서 높은 편이었다.

  • PDF

명충알벌(Trichogramma. chilonis)의 사육 기주에 따른 왕담배나방과 담배나방에 대한 발육반응 및 기생률 (Developmental Performance and Parasitism of Trichogramma chilonis Ishii on Eggs of the Oriental Tobacco Budworm, Helicoverpa assulta(Guenee), and the American Bollworm, Helicoverpa armigera (Hubner) Depending on Previous Hosts)

  • 최만영;김정환;변영웅;김황용;김용헌
    • 한국응용곤충학회지
    • /
    • 제49권4호
    • /
    • pp.393-400
    • /
    • 2010
  • 담배나방 알(HAs)을 기주로 사육된 명충알벌(TC)을 담배나방(HAs-HAs)과 왕담배나방(HAs-HAr) 알에, 줄알락명나방(CC) 알을 기주로 사육된 TC를 왕담배나방(CC-HAr) 알과 담배나방(CC-HAs)에, 왕담배나방(HAr) 알을 기주로 사육된 TC를 왕담배나방(HAr-HAr)과 담배나방(HAr-HAs)에 대한 기생률, 발육기간, 우회율, 번식량을 실험실 조건(온도 $25{\pm}2^{\circ}C$; 습도 50~70%; 광기간 16L-8D)에서 조사하였다. TC는 직전에 어떤 기주에 사육했는가에 따라 시험 기주에서 나타나는 발육반응이 다르다는 것을 알 수 있었으며, 흔히 직전기주와 시험기주의 크기에 의해 발육기간 우회율, 기생률, 번식량이 차이를 보였으며, 가장 큰 차이를 보인 것은 기생률과 번식량인 것으로 나타났다. 기생률은 가장 낮은 CC-HAr의 $10.1{\pm}2.05%$에서 가장 높은 HAr-HAs의 $47.0{\pm}2.09%$로 나타나 처리 간에 유의한 차이를 보였으며, 우회율에서도 유사한 경향을 보였다. 발육기간은 직전기주의 크기가 가장 작고 시험기주의 크기가 가장 큰 CC-HAr처리에서 가장 짧았다. 처리에 따른 TC의 번식량은 HAr-HAs에서 가장 높았으며($93.9{\pm}6.87$마리), 가장 낮은 처리는 CC-HAs($18.4{\pm}6.36$마리)였다.

주거용 건축물의 실내 라돈농도 경감방안에 관한 연구(I) -Test Cell Study (A Study on Mitigation Methods of Indoor Radon Concentration in Residential Buildings(I) - Test Cell Study)

  • 차동원
    • KIEAE Journal
    • /
    • 제1권2호
    • /
    • pp.21-28
    • /
    • 2001
  • Naturally-ocurring short-lived decay products of radon gas in indoor air are the dominant source of ionizing radiation exposure to the general public. It is written in BEIR VI Report(l999l the radon progeny were identified as the second cause of lung cancer next to cigarette or 10 % to 14 %(15,400 to 21,800 persons p.a.) of all lung cancer deaths in USA. Indoor radon concentrations in houses typically result from radon gaining access to houses mainly from the underlying soil. In the States, they have "Indoor Radon Abatement Act" which was converted from "Toxic Substance Control Act" in 1988 to establish the national long-term goal that indoor air should be as free of radon as the ambient air outside of buildings. To review and study techniques for controlling radon, two test cells were constructed for a series of tests and are under measuring indoor and soil gas (underneath of floor slab)radon concentrations according to EPA's measurement protocol. In this paper, important theoretical studies are previewed and the following paper will explain the test results and confirm the theories reviewed to find out suitable coefficients. On the basis of test analysis, it will be described and evaluated various techniques that can be used to mitigate elevated indoor concentration of radon including the control of radon and its decay products.

  • PDF

Chemical Properties and Fiber Dimension of Eucalyptus pellita from The 2nd Generation of Progeny Tests in Pelaihari, South Borneo, Indonesia

  • Lukmandaru, Ganis;Zumaini, Umi Farah;Soeprijadi, Djoko;Nugroho, Widyanto Dwi;Susanto, Mudji
    • Journal of the Korean Wood Science and Technology
    • /
    • 제44권4호
    • /
    • pp.571-588
    • /
    • 2016
  • Eucalyptus pellita F. Muell is one of pulp woods that is being developed through breeding plantation programs in Indonesia. The research aimed at exploring the chemical and morphological characteristics of fiber, and to determine the rank of plus trees from 4 provenances based on the suitability for pulps. The materials included the plus trees of E. pellita (9 years) from the 2nd generation of progeny tests in Pelaihari, South Borneo. Wood properties under investigation included the chemical properties and morphological fiber characteristics (fiber dimensions and its derived properties). In the present study, data were analyzed using descriptive statistic, Analytic Hierarchy Process (AHP) and Pearson's correlation. Results showed that the chemical properties of E. pellita, i.e. the contents of ethanol-toluene extractives, hot water soluble extractives, holocellulose, alphacelullose, and lignin were $3.08{\pm}1.00%$, $1.41{\pm}0.38%$, $75.26{\pm}2.58%$, $49.02{\pm}2.88%$, and $29.49{\pm}1.86%$, respectively. The average values of wood fiber morphology were $1.02{\pm}0.08$ mm (fiber length), $13.25{\pm}1.64{\mu}m$ (fiber diameter), of $6.94{\pm}1.70{\mu}m$ (lumen diameter), $3.15{\pm}0.52{\mu}m$ (fiber wall thickness), $0.97{\pm}0.30$ (Runkel ratio), $0.57{\pm}0.10$ (Luce's shape factor), $78.21{\pm}10.34$ (slenderness ratio) and $130.91{\pm}33.77{\times}10^3{\mu}m^3$ (solids factor). The AHP scoring rank indicated that the best individuals were 28.4.3.28 (Kiriwo Utara), 12.1.5.28 (North Kiriwo), 19.11.5.45 (Serisa Village), 3.8.4.9 (South Kiriwo), and 6.6.3.15 (South Kiriwo). Pearson correlation analysis showed significant correlations between the levels of fiber length with alpha-cellulose content (r = 0.39) as well as the fiber length with ethanol-toluene extractive contents (r = -0.41).

가평지역(加平地域) 잣나무 풍매차대검정림(風媒差代儉定林)의 가계별(家系別) 생장(生長)과 생리적(生理的) 특성(特性)에 관한 연구(硏究) (A Study on Growth and Physiological Characteristic for Families in Open-pollinated Progeny Test Forest of Pinus koraiensis in Kapyung Site)

  • 정동준;신만용;장용석;전상근
    • 한국산림과학회지
    • /
    • 제90권1호
    • /
    • pp.124-132
    • /
    • 2001
  • 본 연구는 경기도 가평지역에 조성한 잣나무 차대검정림을 대상으로 가계별 영양생장에 미치는 생리적 특성을 구명하고자 수행하였다. 연구대상지의 18년생 잣나무 차대검정림의 가계별 생장은 평균수고 5.9m, 평균흉고직경은 8.9cm, 평균흉고단면적은 $12.0m^2$, ha당 평균재적은 $46.5m^3$였다. 전체 25개 가계의 생장을 상 중 하로 구분한 결과 가장 우수한 생장을 보인 가계는 20번 가계였으며, 평균적인 생장을 보인 가계는 10번 가계, 그리고 가장 저조한 생장을 보인 가계는 3번 가계로 판명되었다. 이러한 가계별 생장특성은 광도 증가에 따fms 잣나무의 제철별 광합성속도의 변화와 일치하는 경향을 보였으며, 이는 4계절(겨울 봄 여름 가을) 모두 동일한 결과를 보였다. 계절별 광합성 분석결과에 의하면 겨울에서 봄을 지나 여름으로 갈수록 광합성속도가 증가함을 알 수 있었다. 또한 엽록소 함량도 계절별로 같은 경향을 보였다. 특히 생장이 가장 우수한 20번 가계가 다른 가계들보다도 높은 엽록소 함량을 나타냈으며 엽장(葉長), 침엽의 두께, 동화 기관(同化器官)의 중량 및 기공 수에서도 더 높은 값을 나타내었다. 반면에 수분이용 효율은 모든 계절에서 가장 생장이 저조한 3번 가계가 다른 가계보다 높게 나타나 광합성과는 반대의 경향을 보였으며, 계절별로는 겨울철에서 생장기간인 봄철 및 여름철을 경과하면서 증가하는 것으로 나타났다.

  • PDF

Genetic correlation between live body measurements and beef cutability traits in Hanwoo steers

  • Choy, Yun Ho;Lee, Jae Goo;Mahboob, Alam;Choi, Tae Jeong;Rho, Seung Hee
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제30권8호
    • /
    • pp.1074-1080
    • /
    • 2017
  • Objective: The growth, carcass and retail cut yield records on 1,428 Hanwoo steers obtained through progeny testing were analyzed in this study, and their heritability and genetic relationships among the traits were estimated using animal models. Methods: Two different models were compared in this study. Each model was fitted for different fixed class effects, date of slaughter for carcass traits and batch of progeny test live measurement traits, and a choice of covariates (carcass weight in Model 1 or backfat thickness in Model 2) for carcass traits. Results: The differences in body composition among individuals were deemed being unaffected by their age at slaughter, except for carcass weight and backfat thickness. Heritability estimates of body size measurements were 0.21 to 0.36. Heritability estimates of retail cut percentage were high (0.56 from Model 1 and 0.47 from Model 2). And the heritability estimates for loin muscle percentage were 0.36 from Model 1 and 0.42 from Model 2, which were high enough to consider direct selection on carcass cutability traits as effective. The genetic correlations between body size measurements and retail cut ratio (RCR) were close to zero. But, some negative genetic correlations were found with chest girths measured at yearling (Model 1) or at 24 months of age or with chest widths. Loin muscle ratio (LMR) was genetically negatively correlated with body weights or body size measurements, in general in Model 1. These relationships were low close to zero but positive in Model 2. Phenotypic correlation between cutability traits (RCR, LMR) and live body size measurements were moderate and negative in Model 1 while those in Model 2 were all close to zero. Conclusion: Therefore, the body weights or linear body measurements at an earlier age may not be the most desirable selection traits for exploitation of correlated responses to improve loin muscle or lean meat yield.

수정란 배양 기술을 이용한 형질전환 닭 생산 (Production of Transgenic Chicken by Using Embryo Culture Techniques)

  • I. S. Jeon;D. H. Yoon;E. W. Park;W. G. Nho;C. H. Choi;J. H. Lee;H. H. Lee;I. C. Cheong;J. Y. Han
    • 한국가금학회지
    • /
    • 제28권2호
    • /
    • pp.125-133
    • /
    • 2001
  • The goal of this paper was to examine the qualify zygote-acquiring method for in-vitro culture and the in-vitro culture method of the acquired zygote from a technological perspective. We have reported the results on the introduction of foreign DNAs using the described culturing method. After performing in-vitro and surrogate eggshell culture on a zygote acquired from the abdomen of a hen, 25.8% hatchability was acquired. After microinjecting foreign DNAs into the acquired zygote and performing in-vitro and surrogate eggshell culture using the same method, 13.1∼11.7% hatchability was acquired. Having compared the developments of the control subjects and the experimental subjects, the viability of the experimental subjects on the 4∼5th day of culturing was much lower compared to that of the control subjects. This is a result that shows that the microinjection process of foreign DNAs might have a negative effect on the existence of the embryo; therefore, various technical attempts should be made to minimize such negative effects. Having microinjected foreign DNAs into the zygote of a hen to produce transgenic chickens, 3 transgenic founders were Produced and 70 G1 progeny were produced as a result of the progeny test that had been performed to the present.

  • PDF

형질전환 상추에서 배추 Glutathione Reductase 유전자의 발현 (Expression of Chinese Cabbage Glutathione Reductase Gene in Lettuce (Lactuca sativa L.))

  • 정재동;김창길;조진기
    • 식물조직배양학회지
    • /
    • 제25권4호
    • /
    • pp.267-271
    • /
    • 1998
  • 상추의 자엽 조직을 GR 유전자가 도입된 A. tumefaciens LBA 4404와 2일간 공동배양후, carbenicillin 500mg/L, kanamycin 50mg/L NAA 0.1mg/L와 2iP 1.0 mg/L가 함유된 MS 재분화배지에 옳겨 약 4주후에 kanamycin 저항성 개체를 얻었다. 형질전환된 것으로 추정되는 식물체는 kanamycin 100mg/L가 함유된 MS선발배지에서 생존하였다. PCR 분석결과, GR 유전자가 형질전환체의 게놈상에 삽입되어 있음을 확인하였다. 형질전환체의 Southern blot 분석을 통하여 ECL-labelling된 GR 유전자와 동일한 것으로 판단되는 약 1.8 kb 위치에서 밴드를 확인할 수 있었다. RT-PCR 분석으로 GR 유전자가 전사됨을 확인할 수 있었다. 개화후 이들 개체의 종자를 받아 NPTII 유전자의 발현여부를 조사한 결과 R$_1$ 세대에서도 NPTII 유전자가 발현됨을 확인하였다.

  • PDF