• Journal of Physiology & Pathology in Korean Medicine
• /
• v.18 no.5
• /
• pp.1317-1321
• /
• 2004

The differentiation of the sebaceous gland is remarkably species-specific and sebocytes may play crucial parts in the pathophysiologic processes and disorders of pilosebaceous unit SZ95 cell is an immortalized human sebaceous gland cell line that shows characteristics of normal human sebocytes, In this study, we investigated the effect of testosterone and the anti-androgenic effect of 13-cis-retinoic acid (13-cis-RA) on lipid synthesis in SZ95 cells. Cytoplasmic lipid droplets were shown by Oil-red staining. The majority of the SZ95 cells positively labeled with Oil-red dye, while HaCaT cells negatively labeled with Oil red dye. Total lipid level of SZ96 cells is higher 4 times than that of HaCat cells. Testosterone markedly increased 2 times lipid synthesis of SZ95 cells in compared with control. 13-cis-RA significantly inhibited lipid synthesis and cell proliferation in SZ95 cells. Combined treatment with testosterone and 13-cis-RA resulted in a lower total lipid levels than that with androgen alone. In conclusion, SZ95 cells well resembled the morphologic and functional characteritics of normal human sebocytes. This in vitro model could provide a valuable tool for the study of sebocytes with a key role in pathophysiology and differentiation of sebaceous glands.

• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.141.1-141.1
• /
• 2003

The inhibitory effect of 13(E)-Labd-13-ene-8$\alpha$ 15-diol(1), isolated from the ethanol extract of Brachyglottis monroi, on the proliferation of human breast cancer MDA-MB231 cells was examined. Compound (1) at concentration as high as 16$\mu$/$m\ell$ has inhibited the proliferation of MDA-MB231 and this cytotoxic effect was increased in a time and dose-dependent manners. (omitted)

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.6
• /
• pp.1270-1276
• /
• 2002

Compounds 1 - 12 were tested for their growth inhibitory effects against tumor cell lines using two different 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and sulforhodamine B protein (SRB) assays and antimicrobial activity. The cytotoxic activity of methyl-4-[{(2E)-3,7-dimethyl-2,6-octadienyl}oxy]-3-methoxy benzoate (1) exhibit more active than that of 5-fluorouracil (11) on human oral epithelioid carcinoma (KB, ATCC No. OCL 17) cell lines. But this compound (1) on human skin melanoma (SK-MEL-3, HBT 69) cell lines shows less active than that of adriamycin (12). However, compound 9 showed the antimicrobial activity against S. epidermidis (MIC, 15.625 ㎍/㎖), S. aureus, C. albicans (MIC, 31.25 ㎍/㎖), S. mutans, S. typhimurium, P. putida (MIC. 125 ㎍/㎖) and P. aeruginosa (MIC, 500 ㎍/㎖).

• The Journal of Asian Finance, Economics and Business
• /
• v.7 no.9
• /
• pp.583-591
• /
• 2020

In recent years, the quality of auditing human resources in domestic auditing firms in Hanoi has certain limitations in which professional skills of auditors have not been given adequate attention. The number of customers at local auditing firms is forecast to increase gradually. Therefore, the quantity and quality of auditors need to be improved to meet requirements of the workload. Data for this research were collected through surveying 120 customers of domestic auditing firms in Hanoi on the 5-point Likert scale. Based on the collected data, we employ both qualitative and quantitative methodology along with some tools such as descriptive statistics, Cronbach's Alpha, T-test and ANOVA to analysis, evaluate and measure the professional skills of auditors in local auditing firms in Hanoi. The results show that the professional skills of auditors in auditing firms include 17 attributes. The majority of respondents show appreciation for the professionalism and professional competence of auditors, while, they think that critical thinking skills and creative thinking skills are still limited and need to be improved. These findings, not only help auditors to improve themselves, but also provide solutions for managers of domestic auditing firms in Hanoi to enhance quality of audited human resources.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.20 no.4
• /
• pp.919-923
• /
• 2006

Sebum is an oily substance produced by sebaceous glands in human skin. The differentiation of the sebaceous gland is remarkably species-specific and sebocytes may play crucial parts in the pathophysiologic processes and disorders of pilosebaceous unit. In this study, we investigated the inhibitory effect of Ficis folium on the lipid production using the human sebocyte cell line 5195. Our results showed that Ficis folium significantly inhibits synthesis of sebaceous lipids such as cholesterol esters, triglycerides, and total lipid. And Ficis folium supressed cytoplasmic lipid droplets. On the other hand, Ficis folium didn't effect on cell proliferation. These results suggest treatment with Ficis folium resulted in a lower lipid synthesis in SZ95 cells without cytotoxicity.

• Journal of the Korean Professional Engineers Association
• /
• v.37 no.6
• /
• pp.54-57
• /
• 2004

This study attempts to review the theories and systems related to the policy for scientific and engineering human resources, to delve into the relevant documents and case studies, to carry out a survey, and to compare the Korean situation with those of advanced countries. This will verify the problems in current policies and help draw up the plans for future policies.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.22 no.2
• /
• pp.371-376
• /
• 2008

In this study, we have investigated the hypo-pigmentary mechanism of methanol extract of Forsythiae Fructus in human melanocyte cell line, HM3KO. Treatment of HM3KO cells with Forsythiae Fructus extract markedly inhibited melanin biosynthesis in a dose-dependent manner. Decreased melanin contents occurred through the decrease of tyrosinase protein and activity. The mRNA levels of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also reduced by Forsythiae Fructus extract. Moreover, the level of intracellular cyclic AMP (cAMP) was significantly decreased by treatment of Forsythiae Fructus extract. These results suggest that Forsythiae Fructus reduces melanin synthesis by down regulation of tyrosinase mRNA transcription, and this is closely related to the cAMP-dependent pathway.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.24 no.2
• /
• pp.182-192
• /
• 2014

Objectives: The aim of this study is to analyze the current status of possession of measurement and analytical instruments at work environment monitoring organizations and their relationships with human resources, including the number of professional engineers and evaluation scores resulting from evaluation programs in 2012. Materials: Data for measurement and analytical instruments were gathered by inspectors who had been assigned by the Korea Occupational Safety and Health Agency(KOSHA) and the Ministry of Employment and Labor(MoEL) during the evaluation program for 2012. Data for 11 monitoring instruments and nice pieces of analytical equipment were collected from 103 organizations. Additional data such as the type of service provides and the number of professional engineers employed were also recorded by the inspectors. Evaluation scores could be acquired from KOSHA. Results: Comprehensive Occupational Health Service Providers showed good operation quality, while University or Hospital Subsidiary and Work Environment Monitoring Organizations recorded relatively poor results. Evaluation scores correlated well with the possession of measurement instruments and human resources for each organization. High yields provided by professional engineers also showed statistically-associated contributions to evaluation scores and monitoring instrument possession. Compared with monitoring instruments, the amount of analytical equipment had little positive impact on organizations' competence. Conclusions: The evaluation results for domestic monitoring organizations revealed that human resources, possession of instruments, and the quality of employees were critical factors for operating the corporations. Each organization should give considerable effort to improving their ability to strengtheninternal quality, resulting in high-yield production for workers and employers by providing improved workplace monitoring services.

• Journal of Practical Engineering Education
• /
• v.15 no.3
• /
• pp.681-701
• /
• 2023

The purpose of this study is propose performance indicators analysis and development plans for K University professional graduate school for the training of human resource professionals. Specifically, the main research includes the derivation of performance indicators for K University's professional graduate school, PR plan, academic establishment plan, name change, vision proposal, and curriculum improvement plan for each major. To this end, a survey was conducted on a total of 132 current and former students of K University's professional graduate school. FGI was also conducted with 29 participants. The results of the research are as follows. In the case of key performance indicators, 15 indicators were proposed, taking into account previous research, the characteristics of professional graduate school, surveys, and FGI surveys. Promotion should be continuous, not one-time, and the advantages and benefits of the graduate school should be actively informed to target customers. The proposed name of the professional association is the Korean Association for Employment and Vocational Competency Development. It was found that operating as a convergence society and focusing on convergence research were appropriate. The names of K University's professional graduate school were HR (Human Resources), HRD, and employment and vocational competency development graduate school. As for the vision, it was suggested that a balance between identity and differentiation is needed to flexibly respond to new changes while maintaining existing strengths. As for the proposed improvement of the curriculum by major, it is proposed to reform in a stepwise and gradual manner while maintaining the existing framework to some extent rather than being radical.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.20 no.6
• /
• pp.1561-1566
• /
• 2006

Sebum is secreted due to the effect of androgen, which start to be secreted at puberty. Androgens have profound effects on the physiology of the sebaceous gland, Using the human sebocyte cell line SZ95, we investigated the inhibitory effect of Chelidonii Harba (CH) on the subum production. Our results showed that numerous cytoplasmic lipid droplets were examined by Oil red staining and lipid droplets were increased markedly by testosterone. Cell viability was dose-dependently decreased by CH as compared with untreated cells, while total lipid content and cholesterol slightly were increased by CH. Testosterone significantly stimulated the synthesis of total lipid and the synthesis of specific sebaceous lipids such as cholesterol and triglyceride. Combined treatment with CH and testosterone resulted in a lower lipid synthesis than with testosterone alone. Especially cholesteol content was reduced by combined treatment with CH and testosterone. These results indicate that CH inhibits the testosterone-induced lipid synthesis in SZ95 cells and acts antagonistically to androgen at the cellular level.