• Mycobiology
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• v.48 no.2
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• pp.122-132
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• 2020

Citric acid is a commercially valuable organic acid widely used in food, pharmaceutical, and beverage industries. In this study, 260 yeast strains were isolated from soil, bread, juices, and fruits wastes and preliminarily screened using bromocresol green agar plates for their ability to produce organic acids. Overall, 251 yeast isolates showed positive results, with yellow halos surrounding the colonies. Citric acid production by 20 promising isolates was evaluated using both free and immobilized cell techniques. Results showed that citric acid production by immobilized cells (30-40 g/L) was greater than that of freely suspended cells (8-19 g/L). Of the 20 isolates, two (KKU-L42 and KKU-L53) were selected for further analysis based on their citric acid production levels. Immobilized KKU-L42 cells had a higher citric acid production rate (62.5%), while immobilized KKU-L53 cells showed an ~52.2% increase in citric acid production compared with free cells. The two isolates were accurately identified by amplification and sequence analysis of the 26S rRNA gene D1/D2 domain, with GenBank-based sequence comparison confirming that isolates KKU-L42 and KKU-L53 were Candida tropicalis and Pichia kluyveri, respectively. Several factors, including fermentation period, pH, temperature, and carbon and nitrogen source, were optimized for enhanced production of citric acid by both isolates. Maximum production was achieved at fermentation period of 5 days at pH 5.0 with glucose as a carbon source by both isolates. The optimum incubation temperature for citric acid production by C. tropicalis was 32 ℃, with NH₄Cl the best nitrogen source, while maximum citric acid by P. kluyveri was observed at 27 ℃ with (NH₄)₂ SO₄ as the nitrogen source. Citric acid production was maintained for about four repeated batches over a period of 20 days. Our results suggest that apple and banana wastes are potential sources of novel yeast strains; C. tropicalis and P. kluyveri which could be used for commercial citric acid production.

• The International Journal of Costume Culture
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• v.2 no.2
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• pp.71-79
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• 1999

The purposes of this study were to 1) identify types and usage levels of production 2) classify apparel manufacturers based on production systems and 3) investigate relationship between characteristics of apparel manufacturers and production system. Apparel manufacturer's characteristics included product line and the number of employees. For this study, the questionnaires were administered to 215 apparel manufacturers in metropolitan area from Feb. to Mar. 1998. Employing a sample of 201, data were analyzed by using factor analysis, descriptive statistics, cluster analysis, discriminant analysis, and multivariate analysis of variance(MANOVA). The following are the results or this study : 1. The production system was identified as three types of production system such as the management centered system, the product centered system and the worker centered system. 2. Based on the three types of the production system, apparel manufacturer were classified into manager centered and product centered groups. 3. With respect to product line, men's wear manufactures were operated the most frequently by manager centered and product centered groups. 3. With respect to product line, men's wear manufacturers were operated the most frequently by management centered system and women's wear manufacturers were operated the most frequently by worker centered system. With respect to the number of employees, apparel manufacturers comprising 5∼19 employees were performed the least frequently worker centered system, while those comprising 50∼99 and 100∼299 employees the least frequently worker centered system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.12
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• pp.8719-8727
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• 2015

The purpose of this study is to examine the production efficiency of Rose farm and to explain the factors of the inefficiency. To analysis the production efficiency, SFA(Stochastic Frontier Analysis) and DEA(Data Envelopment Analysis) methods are measured, and then, Tobit regression model is used to analysis the influential factors on the production efficiency. As a result, first, the production efficiency by SFA is 88.4%, and by DEA, results are 78.5% and 85.2% in the CRS and VRS model, respectively. In particular, the production efficiency of the measurement results of the two methods are complementary, it is described in the same order of efficiency of each management body. Second, the results of tobit model shows that 6 input-factors are significant, and seed/nursery and material costs, which have the largest regression coefficient value and positive effect on production efficiency, are the most influential factors. Therefore, the results of this study indicates Rose farm can enhance their management efficiency by increasing amount of the seed/nursery and material costs.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.1
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• pp.1-7
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• 2017

Objective: Three genome-wide association studies (GWAS) and a meta-analysis of GWAS were conducted to explore the genetic mechanisms underlying variation in pig teat number. Methods: We performed three GWAS and a meta-analysis for teat number on three pig populations, including a White Duroc${\times}$Erhualian $F_2$ resource population (n = 1,743), a Chinese Erhualian pig population (n = 320) and a Chinese Sutai pig population (n = 383). Results: We detected 24 single nucleotide polymorphisms (SNPs) that surpassed the genome-wide significant level on Sus Scrofa chromosomes (SSC) 1, 7, and 12 in the $F_2$ resource population, corresponding to four loci for pig teat number. We highlighted vertnin (VRTN) and lysine demethylase 6B (KDM6B) as two interesting candidate genes at the loci on SSC7 and SSC12. No significant associated SNPs were identified in the meta-analysis of GWAS. Conclusion: The results verified the complex genetic architecture of pig teat number. The causative variants for teat number may be different in the three populations

• 제어로봇시스템학회:학술대회논문집
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• 1995.10a
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• pp.259-262
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• 1995

In this paper, a model of an asymptotically reliable serial production line with quailty control devices is introduced and analyzed. By an asymptotic technique and Taylor series expansion, its average production rate is approximated in a closed form. The results are applied to a case study of a surface mount system.

• Korean Journal of Computational Design and Engineering
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• v.16 no.6
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• pp.449-457
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• 2011

The productivity improvement is indispensible to shipbuilding industry for maintaining the world's No. 1. Simulation based production recently has been an issue as prat of efforts to high efficiency production and Korean shipyards requests simulation system tools specialized in a shipbuilding industry. IT convergence project between conventional shipbuilding industry and IT simulation technology has been carried out and integrated simulation framework was proposed as a way to overcome sporadic developments. The framework would provide reusability of kernels and modules and also ensure for expansibilities to other production simulations. The fact that production simulation system should reflect shipyard requirement would be most important. We suggest an analysis process of customer requirements and functional requirements for production simulations. It is partially based on concepts of software engineering and axiomatic design. The process is applied to a design of configuration for simulation framework.

• Journal of Fisheries and Marine Sciences Education
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• v.25 no.6
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• pp.1273-1284
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• 2013

The purpose of this study is to develop vocational English textbooks for vocational high school, especially marine production high school. Vocational English is intended to increase English proficiency in the area of specific industry or jobs. Based on the review of the literature, the study established developed vocational English textbooks for marine production high school based on the modular system. The developing process includes analysis for marine production job, marine production high school and current English textbook for marine production. Based on the analysis, this study developed English contents which marine production students utilize in not only school but also workplace. Also, this study suggests future research directions for effective development and use of the textbooks in the vocational high schools.

• 제어로봇시스템학회:학술대회논문집
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• 1996.10b
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• pp.1024-1027
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• 1996

The cyclic production lines have the first machines starved and the last machine blocked due to the lack or the excess of empty pallets in the feedback buffer. A workpiece in the cyclic production lines is transported on a pallet, and the total sum of pallets in the system does not changed during the system operation time. Therefore, the production rate of the cyclic production lines are dependent on the total number of pallets in the system. In this paper, we suggest the performance analysis method for the cyclic production lines with inspection machines and the optimal total number of pallets in the system that maximizes the production rate of the system. Finally, we validate the suggested methods by simulations.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.389-392
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• 2002

Local Mechanical work is researched which spending unnecessary labors and time due to unnecessary material accumulation and fabricated on site. Accordingly, this study analyzes the work process of mechanical equipments by Lean Production principles. Value Stream Analysis(VSA) uncovers waste factor of the existing method and manufactory production method using Value Stream Mapping(VSM). In the results, this study presents improvement of the existing method problems and negative factors of manufactory production.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.450-459
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• 2017

This study evaluated the effects of culture conditions, including carbon and nitrogen sources, L-monosodium glutamate (MSG), and initial pH, on gamma-aminobutyric acid (GABA) production by Lactobacillus brevis HYE1 isolated from kimchi, a Korean traditional fermented food. L. brevis HYE1 was screened by the production analysis of GABA and genetic analysis of the glutamate decarboxylase gene, resulting in 14.64 mM GABA after 48 h of cultivation in MRS medium containing 1% (w/v) MSG. In order to increase GABA production by L. brevis HYE1, the effects of carbon and nitrogen sources on GABA production were preliminarily investigated via one-factor-at-a-time optimization strategy. As the results, 2% maltose and 3% tryptone were determined to produce 17.93 mM GABA in modified MRS medium with 1% (w/v) MSG. In addition, the optimal MSG concentration and initial pH were determined to be 1% and 5.0, respectively, resulting in production of 18.97 mM GABA. Thereafter, response surface methodology (RSM) was applied to determine the optimal conditions of the above four factors. The results indicate that pH was the most significant factor for GABA production. The optimal culture conditions for maximum GABA production were also determined to be 2.14% (w/v) maltose, 4.01% (w/v) tryptone, 2.38% (w/v) MSG, and an initial pH of 4.74. In these conditions, GABA production by L. brevis HYE1 was predicted to be 21.44 mM using the RSM model. The experiment was performed under these optimized conditions, resulting in GABA production of 18.76 mM. These results show that the predicted and experimental values of GABA production are in good agreement.